Amino acid dipepetide frequency for Mycobacterium phage Hawkeye

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.217AlaAla: 10.217 ± 0.833
0.736AlaCys: 0.736 ± 0.191
5.477AlaAsp: 5.477 ± 0.458
7.962AlaGlu: 7.962 ± 0.559
2.715AlaPhe: 2.715 ± 0.448
7.225AlaGly: 7.225 ± 0.655
1.703AlaHis: 1.703 ± 0.327
4.464AlaIle: 4.464 ± 0.489
5.937AlaLys: 5.937 ± 0.773
6.397AlaLeu: 6.397 ± 0.623
2.393AlaMet: 2.393 ± 0.352
3.682AlaAsn: 3.682 ± 0.391
3.682AlaPro: 3.682 ± 0.496
3.544AlaGln: 3.544 ± 0.406
5.062AlaArg: 5.062 ± 0.63
5.753AlaSer: 5.753 ± 0.494
4.694AlaThr: 4.694 ± 0.51
5.707AlaVal: 5.707 ± 0.525
1.335AlaTrp: 1.335 ± 0.231
3.083AlaTyr: 3.083 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.598CysAla: 0.598 ± 0.192
0.276CysCys: 0.276 ± 0.152
0.598CysAsp: 0.598 ± 0.154
0.644CysGlu: 0.644 ± 0.19
0.276CysPhe: 0.276 ± 0.146
0.828CysGly: 0.828 ± 0.218
0.23CysHis: 0.23 ± 0.095
0.506CysIle: 0.506 ± 0.16
0.506CysLys: 0.506 ± 0.144
0.414CysLeu: 0.414 ± 0.158
0.184CysMet: 0.184 ± 0.083
0.598CysAsn: 0.598 ± 0.171
0.828CysPro: 0.828 ± 0.319
0.138CysGln: 0.138 ± 0.082
0.598CysArg: 0.598 ± 0.14
0.598CysSer: 0.598 ± 0.169
0.506CysThr: 0.506 ± 0.189
0.69CysVal: 0.69 ± 0.17
0.138CysTrp: 0.138 ± 0.086
0.276CysTyr: 0.276 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
5.707AspAla: 5.707 ± 0.461
0.414AspCys: 0.414 ± 0.133
6.397AspAsp: 6.397 ± 1.043
4.97AspGlu: 4.97 ± 0.765
2.347AspPhe: 2.347 ± 0.333
4.556AspGly: 4.556 ± 0.475
1.473AspHis: 1.473 ± 0.264
3.682AspIle: 3.682 ± 0.445
2.991AspLys: 2.991 ± 0.439
5.523AspLeu: 5.523 ± 0.49
1.335AspMet: 1.335 ± 0.229
2.025AspAsn: 2.025 ± 0.326
4.418AspPro: 4.418 ± 0.457
1.565AspGln: 1.565 ± 0.212
3.544AspArg: 3.544 ± 0.458
3.222AspSer: 3.222 ± 0.468
3.268AspThr: 3.268 ± 0.469
3.958AspVal: 3.958 ± 0.453
1.335AspTrp: 1.335 ± 0.241
2.485AspTyr: 2.485 ± 0.326
0.0AspXaa: 0.0 ± 0.0
Glu
7.041GluAla: 7.041 ± 0.612
0.276GluCys: 0.276 ± 0.113
5.016GluAsp: 5.016 ± 0.975
6.167GluGlu: 6.167 ± 0.962
3.129GluPhe: 3.129 ± 0.38
4.464GluGly: 4.464 ± 0.363
1.151GluHis: 1.151 ± 0.238
4.188GluIle: 4.188 ± 0.451
2.991GluLys: 2.991 ± 0.426
6.075GluLeu: 6.075 ± 0.466
1.795GluMet: 1.795 ± 0.313
2.531GluAsn: 2.531 ± 0.276
2.255GluPro: 2.255 ± 0.374
2.255GluGln: 2.255 ± 0.339
4.648GluArg: 4.648 ± 0.688
3.728GluSer: 3.728 ± 0.383
3.452GluThr: 3.452 ± 0.365
4.188GluVal: 4.188 ± 0.493
1.151GluTrp: 1.151 ± 0.267
2.117GluTyr: 2.117 ± 0.394
0.0GluXaa: 0.0 ± 0.0
Phe
2.347PheAla: 2.347 ± 0.418
0.138PheCys: 0.138 ± 0.074
2.439PheAsp: 2.439 ± 0.342
2.255PheGlu: 2.255 ± 0.276
0.874PhePhe: 0.874 ± 0.265
3.912PheGly: 3.912 ± 0.467
0.69PheHis: 0.69 ± 0.157
1.427PheIle: 1.427 ± 0.305
1.565PheLys: 1.565 ± 0.328
2.715PheLeu: 2.715 ± 0.399
0.782PheMet: 0.782 ± 0.185
1.565PheAsn: 1.565 ± 0.266
1.979PhePro: 1.979 ± 0.34
1.519PheGln: 1.519 ± 0.226
2.301PheArg: 2.301 ± 0.399
1.703PheSer: 1.703 ± 0.22
1.887PheThr: 1.887 ± 0.254
1.795PheVal: 1.795 ± 0.244
0.506PheTrp: 0.506 ± 0.157
1.289PheTyr: 1.289 ± 0.245
0.0PheXaa: 0.0 ± 0.0
Gly
4.602GlyAla: 4.602 ± 0.777
0.828GlyCys: 0.828 ± 0.233
5.246GlyAsp: 5.246 ± 0.574
4.924GlyGlu: 4.924 ± 0.443
3.037GlyPhe: 3.037 ± 0.381
6.535GlyGly: 6.535 ± 0.987
1.289GlyHis: 1.289 ± 0.26
5.338GlyIle: 5.338 ± 0.683
4.924GlyLys: 4.924 ± 0.434
6.765GlyLeu: 6.765 ± 0.68
1.979GlyMet: 1.979 ± 0.393
3.268GlyAsn: 3.268 ± 0.349
4.326GlyPro: 4.326 ± 0.578
2.209GlyGln: 2.209 ± 0.312
4.004GlyArg: 4.004 ± 0.397
5.845GlySer: 5.845 ± 0.68
5.661GlyThr: 5.661 ± 0.704
5.569GlyVal: 5.569 ± 0.486
1.749GlyTrp: 1.749 ± 0.24
2.485GlyTyr: 2.485 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.749HisAla: 1.749 ± 0.335
0.184HisCys: 0.184 ± 0.091
1.012HisAsp: 1.012 ± 0.222
0.966HisGlu: 0.966 ± 0.241
0.92HisPhe: 0.92 ± 0.174
1.933HisGly: 1.933 ± 0.295
0.322HisHis: 0.322 ± 0.182
0.736HisIle: 0.736 ± 0.175
0.782HisLys: 0.782 ± 0.19
1.749HisLeu: 1.749 ± 0.268
0.322HisMet: 0.322 ± 0.106
0.782HisAsn: 0.782 ± 0.225
1.381HisPro: 1.381 ± 0.241
0.874HisGln: 0.874 ± 0.227
1.335HisArg: 1.335 ± 0.243
1.012HisSer: 1.012 ± 0.258
0.828HisThr: 0.828 ± 0.203
1.243HisVal: 1.243 ± 0.238
0.598HisTrp: 0.598 ± 0.2
1.058HisTyr: 1.058 ± 0.227
0.0HisXaa: 0.0 ± 0.0
Ile
5.2IleAla: 5.2 ± 0.495
0.46IleCys: 0.46 ± 0.143
4.188IleAsp: 4.188 ± 0.497
3.083IleGlu: 3.083 ± 0.352
2.025IlePhe: 2.025 ± 0.313
4.004IleGly: 4.004 ± 0.618
0.828IleHis: 0.828 ± 0.171
3.222IleIle: 3.222 ± 0.457
2.761IleLys: 2.761 ± 0.386
3.268IleLeu: 3.268 ± 0.38
1.427IleMet: 1.427 ± 0.277
2.623IleAsn: 2.623 ± 0.325
3.083IlePro: 3.083 ± 0.398
1.933IleGln: 1.933 ± 0.315
3.222IleArg: 3.222 ± 0.342
2.761IleSer: 2.761 ± 0.355
3.36IleThr: 3.36 ± 0.462
4.142IleVal: 4.142 ± 0.429
1.105IleTrp: 1.105 ± 0.222
1.381IleTyr: 1.381 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
5.615LysAla: 5.615 ± 0.654
0.506LysCys: 0.506 ± 0.165
3.498LysAsp: 3.498 ± 0.521
3.866LysGlu: 3.866 ± 0.406
1.933LysPhe: 1.933 ± 0.29
3.083LysGly: 3.083 ± 0.418
1.058LysHis: 1.058 ± 0.25
3.452LysIle: 3.452 ± 0.431
3.59LysLys: 3.59 ± 0.588
4.004LysLeu: 4.004 ± 0.304
1.151LysMet: 1.151 ± 0.261
1.979LysAsn: 1.979 ± 0.361
2.669LysPro: 2.669 ± 0.364
2.117LysGln: 2.117 ± 0.314
4.832LysArg: 4.832 ± 0.813
3.314LysSer: 3.314 ± 0.282
3.268LysThr: 3.268 ± 0.43
4.004LysVal: 4.004 ± 0.359
0.92LysTrp: 0.92 ± 0.186
1.795LysTyr: 1.795 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
6.535LeuAla: 6.535 ± 0.649
0.782LeuCys: 0.782 ± 0.225
4.372LeuAsp: 4.372 ± 0.457
5.154LeuGlu: 5.154 ± 0.654
2.025LeuPhe: 2.025 ± 0.302
6.673LeuGly: 6.673 ± 0.81
1.565LeuHis: 1.565 ± 0.305
3.037LeuIle: 3.037 ± 0.334
4.832LeuLys: 4.832 ± 0.549
5.799LeuLeu: 5.799 ± 0.479
1.611LeuMet: 1.611 ± 0.315
2.991LeuAsn: 2.991 ± 0.339
3.912LeuPro: 3.912 ± 0.447
2.439LeuGln: 2.439 ± 0.321
4.878LeuArg: 4.878 ± 0.399
5.292LeuSer: 5.292 ± 0.49
4.418LeuThr: 4.418 ± 0.367
4.188LeuVal: 4.188 ± 0.346
0.92LeuTrp: 0.92 ± 0.297
1.565LeuTyr: 1.565 ± 0.284
0.0LeuXaa: 0.0 ± 0.0
Met
3.037MetAla: 3.037 ± 0.393
0.414MetCys: 0.414 ± 0.142
1.289MetAsp: 1.289 ± 0.279
0.92MetGlu: 0.92 ± 0.214
0.828MetPhe: 0.828 ± 0.174
1.289MetGly: 1.289 ± 0.23
0.506MetHis: 0.506 ± 0.167
1.197MetIle: 1.197 ± 0.227
1.012MetLys: 1.012 ± 0.251
1.335MetLeu: 1.335 ± 0.255
0.552MetMet: 0.552 ± 0.186
0.966MetAsn: 0.966 ± 0.212
1.473MetPro: 1.473 ± 0.243
1.105MetGln: 1.105 ± 0.23
1.565MetArg: 1.565 ± 0.223
2.209MetSer: 2.209 ± 0.328
1.841MetThr: 1.841 ± 0.261
0.828MetVal: 0.828 ± 0.266
0.46MetTrp: 0.46 ± 0.127
0.69MetTyr: 0.69 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
4.004AsnAla: 4.004 ± 0.411
0.23AsnCys: 0.23 ± 0.107
1.933AsnAsp: 1.933 ± 0.338
2.485AsnGlu: 2.485 ± 0.344
1.197AsnPhe: 1.197 ± 0.264
3.866AsnGly: 3.866 ± 0.52
0.874AsnHis: 0.874 ± 0.182
2.163AsnIle: 2.163 ± 0.344
1.887AsnLys: 1.887 ± 0.278
2.807AsnLeu: 2.807 ± 0.352
1.151AsnMet: 1.151 ± 0.173
1.427AsnAsn: 1.427 ± 0.255
2.623AsnPro: 2.623 ± 0.406
2.071AsnGln: 2.071 ± 0.392
2.899AsnArg: 2.899 ± 0.385
2.393AsnSer: 2.393 ± 0.316
2.071AsnThr: 2.071 ± 0.336
2.853AsnVal: 2.853 ± 0.361
1.058AsnTrp: 1.058 ± 0.225
1.519AsnTyr: 1.519 ± 0.3
0.0AsnXaa: 0.0 ± 0.0
Pro
4.786ProAla: 4.786 ± 0.456
0.368ProCys: 0.368 ± 0.149
3.222ProAsp: 3.222 ± 0.398
4.142ProGlu: 4.142 ± 0.365
1.933ProPhe: 1.933 ± 0.261
4.832ProGly: 4.832 ± 0.568
1.105ProHis: 1.105 ± 0.219
2.439ProIle: 2.439 ± 0.332
3.037ProLys: 3.037 ± 0.435
3.636ProLeu: 3.636 ± 0.457
0.736ProMet: 0.736 ± 0.196
2.393ProAsn: 2.393 ± 0.458
2.209ProPro: 2.209 ± 0.325
1.243ProGln: 1.243 ± 0.24
2.209ProArg: 2.209 ± 0.322
3.682ProSer: 3.682 ± 0.455
2.945ProThr: 2.945 ± 0.384
3.36ProVal: 3.36 ± 0.359
1.151ProTrp: 1.151 ± 0.227
1.473ProTyr: 1.473 ± 0.27
0.0ProXaa: 0.0 ± 0.0
Gln
3.129GlnAla: 3.129 ± 0.549
0.23GlnCys: 0.23 ± 0.099
2.025GlnAsp: 2.025 ± 0.285
1.887GlnGlu: 1.887 ± 0.259
1.611GlnPhe: 1.611 ± 0.295
2.163GlnGly: 2.163 ± 0.389
0.874GlnHis: 0.874 ± 0.205
2.393GlnIle: 2.393 ± 0.337
2.577GlnLys: 2.577 ± 0.328
2.899GlnLeu: 2.899 ± 0.328
1.058GlnMet: 1.058 ± 0.196
1.565GlnAsn: 1.565 ± 0.317
1.427GlnPro: 1.427 ± 0.276
1.381GlnGln: 1.381 ± 0.194
2.715GlnArg: 2.715 ± 0.466
2.163GlnSer: 2.163 ± 0.362
1.979GlnThr: 1.979 ± 0.283
2.163GlnVal: 2.163 ± 0.296
0.828GlnTrp: 0.828 ± 0.22
1.058GlnTyr: 1.058 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
5.937ArgAla: 5.937 ± 0.661
0.644ArgCys: 0.644 ± 0.211
3.083ArgAsp: 3.083 ± 0.334
3.774ArgGlu: 3.774 ± 0.517
1.611ArgPhe: 1.611 ± 0.265
4.234ArgGly: 4.234 ± 0.395
1.243ArgHis: 1.243 ± 0.264
4.004ArgIle: 4.004 ± 0.499
4.694ArgLys: 4.694 ± 0.651
4.05ArgLeu: 4.05 ± 0.43
2.071ArgMet: 2.071 ± 0.314
2.991ArgAsn: 2.991 ± 0.505
2.301ArgPro: 2.301 ± 0.381
2.669ArgGln: 2.669 ± 0.371
4.096ArgArg: 4.096 ± 0.613
3.406ArgSer: 3.406 ± 0.47
2.899ArgThr: 2.899 ± 0.355
4.556ArgVal: 4.556 ± 0.483
1.243ArgTrp: 1.243 ± 0.284
2.301ArgTyr: 2.301 ± 0.385
0.0ArgXaa: 0.0 ± 0.0
Ser
6.535SerAla: 6.535 ± 0.578
0.69SerCys: 0.69 ± 0.246
4.372SerAsp: 4.372 ± 0.554
3.958SerGlu: 3.958 ± 0.382
2.255SerPhe: 2.255 ± 0.252
6.397SerGly: 6.397 ± 0.664
1.105SerHis: 1.105 ± 0.259
3.268SerIle: 3.268 ± 0.522
3.36SerLys: 3.36 ± 0.397
3.083SerLeu: 3.083 ± 0.351
1.979SerMet: 1.979 ± 0.286
2.715SerAsn: 2.715 ± 0.35
2.761SerPro: 2.761 ± 0.397
2.347SerGln: 2.347 ± 0.33
2.899SerArg: 2.899 ± 0.357
3.912SerSer: 3.912 ± 0.46
3.544SerThr: 3.544 ± 0.326
3.498SerVal: 3.498 ± 0.431
1.381SerTrp: 1.381 ± 0.293
1.657SerTyr: 1.657 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
5.385ThrAla: 5.385 ± 0.533
0.69ThrCys: 0.69 ± 0.231
3.728ThrAsp: 3.728 ± 0.516
3.82ThrGlu: 3.82 ± 0.389
1.611ThrPhe: 1.611 ± 0.308
5.937ThrGly: 5.937 ± 0.626
1.151ThrHis: 1.151 ± 0.221
2.761ThrIle: 2.761 ± 0.407
3.175ThrLys: 3.175 ± 0.383
3.866ThrLeu: 3.866 ± 0.438
0.874ThrMet: 0.874 ± 0.204
1.979ThrAsn: 1.979 ± 0.287
3.36ThrPro: 3.36 ± 0.502
1.887ThrGln: 1.887 ± 0.262
3.129ThrArg: 3.129 ± 0.363
3.912ThrSer: 3.912 ± 0.556
3.268ThrThr: 3.268 ± 0.448
3.544ThrVal: 3.544 ± 0.469
1.197ThrTrp: 1.197 ± 0.309
1.703ThrTyr: 1.703 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
5.292ValAla: 5.292 ± 0.427
0.828ValCys: 0.828 ± 0.231
4.004ValAsp: 4.004 ± 0.477
4.556ValGlu: 4.556 ± 0.52
1.887ValPhe: 1.887 ± 0.32
4.878ValGly: 4.878 ± 0.641
1.381ValHis: 1.381 ± 0.256
2.899ValIle: 2.899 ± 0.372
3.636ValLys: 3.636 ± 0.482
4.786ValLeu: 4.786 ± 0.603
1.151ValMet: 1.151 ± 0.194
2.301ValAsn: 2.301 ± 0.271
3.774ValPro: 3.774 ± 0.417
2.761ValGln: 2.761 ± 0.373
3.82ValArg: 3.82 ± 0.469
4.372ValSer: 4.372 ± 0.502
4.05ValThr: 4.05 ± 0.463
5.338ValVal: 5.338 ± 0.706
1.012ValTrp: 1.012 ± 0.219
2.025ValTyr: 2.025 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
1.703TrpAla: 1.703 ± 0.321
0.276TrpCys: 0.276 ± 0.116
1.058TrpAsp: 1.058 ± 0.276
0.966TrpGlu: 0.966 ± 0.205
0.598TrpPhe: 0.598 ± 0.209
0.92TrpGly: 0.92 ± 0.195
0.506TrpHis: 0.506 ± 0.119
1.151TrpIle: 1.151 ± 0.234
1.151TrpLys: 1.151 ± 0.242
1.887TrpLeu: 1.887 ± 0.277
0.322TrpMet: 0.322 ± 0.122
1.289TrpAsn: 1.289 ± 0.275
0.874TrpPro: 0.874 ± 0.222
0.736TrpGln: 0.736 ± 0.163
1.473TrpArg: 1.473 ± 0.273
0.828TrpSer: 0.828 ± 0.184
1.243TrpThr: 1.243 ± 0.244
1.151TrpVal: 1.151 ± 0.273
0.23TrpTrp: 0.23 ± 0.132
0.414TrpTyr: 0.414 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.347TyrAla: 2.347 ± 0.44
0.46TyrCys: 0.46 ± 0.149
2.255TyrAsp: 2.255 ± 0.427
2.117TyrGlu: 2.117 ± 0.378
0.874TyrPhe: 0.874 ± 0.198
2.853TyrGly: 2.853 ± 0.365
0.736TyrHis: 0.736 ± 0.162
1.749TyrIle: 1.749 ± 0.32
1.243TyrLys: 1.243 ± 0.22
1.979TyrLeu: 1.979 ± 0.251
0.598TyrMet: 0.598 ± 0.163
1.841TyrAsn: 1.841 ± 0.416
1.565TyrPro: 1.565 ± 0.268
1.335TyrGln: 1.335 ± 0.286
2.577TyrArg: 2.577 ± 0.332
1.611TyrSer: 1.611 ± 0.244
1.795TyrThr: 1.795 ± 0.306
1.979TyrVal: 1.979 ± 0.322
0.46TyrTrp: 0.46 ± 0.119
0.736TyrTyr: 0.736 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (21730 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski