Microbacterium chocolatum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M8MMV9|A0A0M8MMV9_9MICO Abasic site processing protein OS=Microbacterium chocolatum OX=84292 GN=XI38_12245 PE=3 SV=1
MM1 pKa = 7.58LAPAATLALGALVLAGCAGGAPASSSAPGEE31 pKa = 4.09ADD33 pKa = 3.16PQAEE37 pKa = 4.48VIVGSQNEE45 pKa = 4.43PTNLDD50 pKa = 3.56QIFGGSSGVTEE61 pKa = 4.12VFTGNVYY68 pKa = 10.71EE69 pKa = 4.49GLFKK73 pKa = 10.27ITDD76 pKa = 3.77DD77 pKa = 4.04AAVEE81 pKa = 4.06PLLATDD87 pKa = 4.22TEE89 pKa = 4.42VSEE92 pKa = 5.21DD93 pKa = 3.39GLVYY97 pKa = 10.54TFTLQPDD104 pKa = 3.75ATFHH108 pKa = 6.98SGDD111 pKa = 3.78PVDD114 pKa = 5.03AEE116 pKa = 4.05AVKK119 pKa = 10.87YY120 pKa = 10.22SLEE123 pKa = 4.11RR124 pKa = 11.84FVSEE128 pKa = 3.75EE129 pKa = 3.76SIAARR134 pKa = 11.84KK135 pKa = 8.78RR136 pKa = 11.84QLSVIDD142 pKa = 3.85SVEE145 pKa = 4.23VIDD148 pKa = 5.0DD149 pKa = 3.49RR150 pKa = 11.84TVAVTLSQPSISFTYY165 pKa = 9.69NLGYY169 pKa = 10.53VWIVNPAAGDD179 pKa = 3.65LTSAADD185 pKa = 3.78GSGPYY190 pKa = 10.6ALADD194 pKa = 3.61YY195 pKa = 10.76RR196 pKa = 11.84KK197 pKa = 9.24GDD199 pKa = 4.03SIALEE204 pKa = 4.19VNEE207 pKa = 4.98EE208 pKa = 4.27YY209 pKa = 10.02WGEE212 pKa = 3.85APANGGVVYY221 pKa = 10.11QYY223 pKa = 11.22YY224 pKa = 11.08ADD226 pKa = 3.7PTALNNALLTNAVDD240 pKa = 4.68LVTSQSNPDD249 pKa = 3.24SLAEE253 pKa = 3.81FEE255 pKa = 4.35AAGFEE260 pKa = 4.45IIEE263 pKa = 4.47GTSTTKK269 pKa = 10.62EE270 pKa = 3.52LLAFNDD276 pKa = 4.74RR277 pKa = 11.84IAPFDD282 pKa = 4.97DD283 pKa = 3.21IQVRR287 pKa = 11.84KK288 pKa = 9.98AIYY291 pKa = 9.5SAINRR296 pKa = 11.84EE297 pKa = 3.98QLLDD301 pKa = 4.87AIWDD305 pKa = 3.71GRR307 pKa = 11.84GQLIGSMVPPSEE319 pKa = 3.77PWYY322 pKa = 11.0LDD324 pKa = 3.57LADD327 pKa = 4.63NNPYY331 pKa = 10.79DD332 pKa = 3.84PALSEE337 pKa = 4.5EE338 pKa = 4.37LLADD342 pKa = 4.02AGYY345 pKa = 11.47ADD347 pKa = 4.53GFSFTLDD354 pKa = 3.47TPDD357 pKa = 3.65SGVHH361 pKa = 4.14STVAEE366 pKa = 4.24FVKK369 pKa = 10.42TEE371 pKa = 3.95LAKK374 pKa = 11.02VGITVDD380 pKa = 3.24INIITDD386 pKa = 3.59DD387 pKa = 2.98EE388 pKa = 4.96WYY390 pKa = 10.5QKK392 pKa = 11.05VYY394 pKa = 10.79TDD396 pKa = 3.59TDD398 pKa = 3.99FEE400 pKa = 4.38ATLQGHH406 pKa = 5.63VNDD409 pKa = 4.96RR410 pKa = 11.84DD411 pKa = 3.37INFYY415 pKa = 11.17GNPDD419 pKa = 4.21FYY421 pKa = 10.66WGYY424 pKa = 11.37DD425 pKa = 3.45NADD428 pKa = 3.48VQTWLADD435 pKa = 3.46SEE437 pKa = 4.4AASTVEE443 pKa = 4.08EE444 pKa = 3.95QTEE447 pKa = 4.27LVKK450 pKa = 10.75LANQQISDD458 pKa = 3.92DD459 pKa = 3.97AASVWLYY466 pKa = 10.7LNPQLRR472 pKa = 11.84VAAPGIGGVPEE483 pKa = 4.34NGLNSLFYY491 pKa = 11.06VYY493 pKa = 10.11DD494 pKa = 3.47IEE496 pKa = 4.87KK497 pKa = 10.46AASS500 pKa = 3.16

Molecular weight: 53.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M8MQM2|A0A0M8MQM2_9MICO LacI family transcriptional regulator OS=Microbacterium chocolatum OX=84292 GN=XI38_00950 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.97GRR40 pKa = 11.84TEE42 pKa = 4.38LSAA45 pKa = 4.86

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2721

0

2721

868408

37

2114

319.2

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.912 ± 0.062

0.484 ± 0.01

6.588 ± 0.039

5.743 ± 0.039

3.089 ± 0.025

8.871 ± 0.039

1.972 ± 0.023

4.507 ± 0.029

1.73 ± 0.031

9.992 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.742 ± 0.019

1.758 ± 0.021

5.521 ± 0.039

2.584 ± 0.026

7.825 ± 0.055

5.253 ± 0.028

5.941 ± 0.035

9.078 ± 0.04

1.506 ± 0.021

1.903 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski