Pacific flying fox faeces associated gemycircularvirus-10
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTP6|A0A140CTP6_9VIRU RepA OS=Pacific flying fox faeces associated gemycircularvirus-10 OX=1795989 PE=3 SV=1
MM1 pKa = 7.67 PLAVNAQHH9 pKa = 7.09 FLLTYY14 pKa = 10.77 AHH16 pKa = 6.8 VEE18 pKa = 4.23 GNDD21 pKa = 4.12 DD22 pKa = 4.83 KK23 pKa = 11.84 PEE25 pKa = 4.66 LDD27 pKa = 3.51 PFRR30 pKa = 11.84 IVDD33 pKa = 3.47 VLGAKK38 pKa = 9.68 GAEE41 pKa = 4.05 CIVARR46 pKa = 11.84 EE47 pKa = 4.39 FYY49 pKa = 9.97 PNTGGFHH56 pKa = 5.74 FHH58 pKa = 6.18 VFCSFEE64 pKa = 4.49 RR65 pKa = 11.84 NFRR68 pKa = 11.84 SRR70 pKa = 11.84 KK71 pKa = 9.65 ADD73 pKa = 3.32 VFDD76 pKa = 3.53 VDD78 pKa = 5.1 GYY80 pKa = 10.96 HH81 pKa = 7.21 PNIEE85 pKa = 4.12 PSRR88 pKa = 11.84 KK89 pKa = 8.63 NAAGGFDD96 pKa = 4.07 YY97 pKa = 9.3 ATKK100 pKa = 10.78 DD101 pKa = 3.18 GDD103 pKa = 3.7 IVAGGLEE110 pKa = 4.28 RR111 pKa = 11.84 PSRR114 pKa = 11.84 TRR116 pKa = 11.84 GRR118 pKa = 11.84 AGTTGAQATWGEE130 pKa = 3.89 ITAAEE135 pKa = 4.18 SAEE138 pKa = 4.28 EE139 pKa = 4.01 FWRR142 pKa = 11.84 LCEE145 pKa = 3.96 EE146 pKa = 4.93 LDD148 pKa = 3.94 PKK150 pKa = 11.64 SMVCNFPALSKK161 pKa = 9.48 FAEE164 pKa = 4.1 WRR166 pKa = 11.84 FRR168 pKa = 11.84 PIPVPYY174 pKa = 10.04 ASPDD178 pKa = 3.48 GVFDD182 pKa = 3.56 TSGYY186 pKa = 9.74 PIIEE190 pKa = 4.28 EE191 pKa = 4.04 WRR193 pKa = 11.84 SSVFDD198 pKa = 4.0 EE199 pKa = 4.2 YY200 pKa = 11.35 SGRR203 pKa = 11.84 GEE205 pKa = 4.34 SYY207 pKa = 10.05 TYY209 pKa = 9.51 LTSLVLFGPTRR220 pKa = 11.84 LGKK223 pKa = 6.32 TTWARR228 pKa = 11.84 SLGSHH233 pKa = 6.88 IYY235 pKa = 10.5 FGGLFSAGEE244 pKa = 3.96 AMRR247 pKa = 11.84 CPEE250 pKa = 4.14 AEE252 pKa = 4.08 YY253 pKa = 10.59 AIMDD257 pKa = 5.62 DD258 pKa = 3.07 IAGGIKK264 pKa = 10.03 FFPRR268 pKa = 11.84 FKK270 pKa = 10.86 DD271 pKa = 3.16 WLGCQAQFQLKK282 pKa = 9.74 VLYY285 pKa = 10.02 KK286 pKa = 10.74 EE287 pKa = 3.79 PALYY291 pKa = 10.33 DD292 pKa = 3.25 WGKK295 pKa = 9.31 PCIWCYY301 pKa = 10.84 NVDD304 pKa = 3.46 PRR306 pKa = 11.84 AGMSVEE312 pKa = 4.32 DD313 pKa = 4.27 IEE315 pKa = 4.34 WLEE318 pKa = 4.17 GNCVFVEE325 pKa = 3.98 ITSPIFHH332 pKa = 7.38 ANTEE336 pKa = 4.14
Molecular weight: 37.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.877
IPC2_protein 4.978
IPC_protein 4.914
Toseland 4.825
ProMoST 4.978
Dawson 4.889
Bjellqvist 5.041
Wikipedia 4.736
Rodwell 4.8
Grimsley 4.736
Solomon 4.876
Lehninger 4.838
Nozaki 5.003
DTASelect 5.13
Thurlkill 4.813
EMBOSS 4.774
Sillero 5.067
Patrickios 3.668
IPC_peptide 4.889
IPC2_peptide 5.067
IPC2.peptide.svr19 5.004
Protein with the highest isoelectric point:
>tr|A0A140CTP5|A0A140CTP5_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated gemycircularvirus-10 OX=1795989 PE=3 SV=1
MM1 pKa = 7.87 AYY3 pKa = 10.08 ARR5 pKa = 11.84 SSTRR9 pKa = 11.84 RR10 pKa = 11.84 SYY12 pKa = 10.85 RR13 pKa = 11.84 RR14 pKa = 11.84 SSRR17 pKa = 11.84 YY18 pKa = 8.8 ARR20 pKa = 11.84 RR21 pKa = 11.84 KK22 pKa = 8.87 IPHH25 pKa = 4.55 TRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 YY30 pKa = 8.87 VKK32 pKa = 8.37 RR33 pKa = 11.84 TSRR36 pKa = 11.84 TPYY39 pKa = 8.78 RR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 8.27 MTQKK46 pKa = 10.65 KK47 pKa = 9.43 ILNLTSIKK55 pKa = 10.5 KK56 pKa = 9.71 RR57 pKa = 11.84 DD58 pKa = 3.61 TMLSLTNVIADD69 pKa = 4.14 RR70 pKa = 11.84 SRR72 pKa = 11.84 PTSYY76 pKa = 10.5 TSNDD80 pKa = 3.02 AVLSGGAQPYY90 pKa = 9.23 IIPWVATARR99 pKa = 11.84 TTTVNGTKK107 pKa = 9.95 QGTIATSATRR117 pKa = 11.84 SSTTCYY123 pKa = 10.16 IRR125 pKa = 11.84 GLRR128 pKa = 11.84 EE129 pKa = 4.3 DD130 pKa = 3.39 IEE132 pKa = 4.78 INVFTGCPWQWRR144 pKa = 11.84 RR145 pKa = 11.84 IVFFSKK151 pKa = 10.79 GLTNAVGTPGSNFYY165 pKa = 10.43 YY166 pKa = 10.78 FNVTAEE172 pKa = 4.26 DD173 pKa = 3.57 YY174 pKa = 10.72 RR175 pKa = 11.84 RR176 pKa = 11.84 TVNEE180 pKa = 3.7 IYY182 pKa = 10.51 GAYY185 pKa = 10.44 ANGLNAFLFKK195 pKa = 10.25 GTEE198 pKa = 4.03 GSDD201 pKa = 3.11 WNDD204 pKa = 3.1 LMIAPVDD211 pKa = 3.67 TTRR214 pKa = 11.84 VSVLYY219 pKa = 10.78 DD220 pKa = 3.16 KK221 pKa = 10.97 TRR223 pKa = 11.84 TIASGNEE230 pKa = 3.37 EE231 pKa = 4.02 GVIRR235 pKa = 11.84 KK236 pKa = 9.39 YY237 pKa = 11.38 KK238 pKa = 8.64 MWHH241 pKa = 5.6 GVNKK245 pKa = 10.27 NLTYY249 pKa = 11.09 DD250 pKa = 3.81 DD251 pKa = 5.41 DD252 pKa = 4.42 EE253 pKa = 5.82 FGGDD257 pKa = 3.33 EE258 pKa = 4.33 TTSNLSTLSKK268 pKa = 10.5 VGCGDD273 pKa = 3.41 MMVVDD278 pKa = 3.81 IFKK281 pKa = 10.46 PRR283 pKa = 11.84 GGSAGEE289 pKa = 3.88 DD290 pKa = 2.51 RR291 pKa = 11.84 MAFKK295 pKa = 10.8 PNATLYY301 pKa = 8.81 WHH303 pKa = 6.99 EE304 pKa = 4.27 KK305 pKa = 9.3
Molecular weight: 34.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.272
IPC2_protein 9.575
IPC_protein 9.94
Toseland 10.058
ProMoST 9.867
Dawson 10.306
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.57
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.058
DTASelect 10.014
Thurlkill 10.131
EMBOSS 10.482
Sillero 10.218
Patrickios 9.823
IPC_peptide 10.321
IPC2_peptide 8.843
IPC2.peptide.svr19 8.604
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
879
238
336
293.0
32.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.191 ± 0.665
1.934 ± 0.403
5.688 ± 0.18
6.371 ± 1.125
6.143 ± 1.166
8.532 ± 0.41
1.82 ± 0.351
4.551 ± 0.404
4.323 ± 0.668
5.575 ± 0.266
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.706 ± 0.395
3.868 ± 0.691
5.575 ± 0.969
1.365 ± 0.052
7.85 ± 0.995
6.598 ± 0.532
7.395 ± 1.67
5.802 ± 0.062
2.162 ± 0.221
4.551 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here