Staphylococcus phage phiJB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; Staphylococcus virus phiJB

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9BAZ6|A0A0N9BAZ6_9CAUD Uncharacterized protein OS=Staphylococcus phage phiJB OX=1698421 GN=phiJB_ORF35 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.23KK3 pKa = 10.48FNVQITYY10 pKa = 9.56TGMIEE15 pKa = 3.91EE16 pKa = 4.54TIEE19 pKa = 4.21AEE21 pKa = 4.2SLEE24 pKa = 4.25EE25 pKa = 4.61AEE27 pKa = 5.29FEE29 pKa = 4.14ADD31 pKa = 3.22VIARR35 pKa = 11.84MEE37 pKa = 4.19VPFDD41 pKa = 3.66CDD43 pKa = 2.81EE44 pKa = 4.26YY45 pKa = 11.01EE46 pKa = 4.04IYY48 pKa = 11.11VDD50 pKa = 4.7VEE52 pKa = 4.21QEE54 pKa = 3.46NDD56 pKa = 2.98

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9BAW8|A0A0N9BAW8_9CAUD Uncharacterized protein OS=Staphylococcus phage phiJB OX=1698421 GN=phiJB_ORF23 PE=4 SV=1
MM1 pKa = 7.57SIVKK5 pKa = 10.22INGKK9 pKa = 8.42PYY11 pKa = 10.87KK12 pKa = 9.54FTEE15 pKa = 4.42HH16 pKa = 6.09EE17 pKa = 4.0NEE19 pKa = 4.33LIKK22 pKa = 11.15KK23 pKa = 9.98NGLTPGMVAKK33 pKa = 10.32RR34 pKa = 11.84VRR36 pKa = 11.84GGWALLEE43 pKa = 4.48ALHH46 pKa = 6.68APYY49 pKa = 11.09GMRR52 pKa = 11.84LAEE55 pKa = 4.07YY56 pKa = 10.46KK57 pKa = 10.56EE58 pKa = 4.03IVLARR63 pKa = 11.84IMQRR67 pKa = 11.84EE68 pKa = 3.79ARR70 pKa = 11.84EE71 pKa = 3.84RR72 pKa = 11.84EE73 pKa = 3.86IARR76 pKa = 11.84QRR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 9.29EE81 pKa = 3.76AEE83 pKa = 3.86LRR85 pKa = 11.84KK86 pKa = 10.04KK87 pKa = 10.35KK88 pKa = 9.92PHH90 pKa = 6.47LFNVPQKK97 pKa = 10.45HH98 pKa = 4.8PRR100 pKa = 11.84GRR102 pKa = 11.84YY103 pKa = 8.83ACYY106 pKa = 10.52LMEE109 pKa = 5.36NDD111 pKa = 4.09IFVKK115 pKa = 10.41VKK117 pKa = 10.3KK118 pKa = 10.51

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13475

20

1047

192.5

22.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.677 ± 0.438

0.475 ± 0.08

6.36 ± 0.287

7.547 ± 0.539

4.453 ± 0.206

5.9 ± 0.351

1.803 ± 0.158

7.51 ± 0.206

9.321 ± 0.397

7.473 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.18

6.479 ± 0.277

2.783 ± 0.2

3.8 ± 0.216

4.045 ± 0.231

5.811 ± 0.334

5.974 ± 0.297

6.16 ± 0.341

1.143 ± 0.162

4.586 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski