Streptococcus phage CHPC926
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L2JXR3|A0A1L2JXR3_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC926 OX=1913082 GN=CHPC926_0037 PE=4 SV=1
MM1 pKa = 7.13 VNWVDD6 pKa = 4.56 ANGNDD11 pKa = 4.18 LPDD14 pKa = 4.84 GADD17 pKa = 3.18 QDD19 pKa = 4.63 FKK21 pKa = 11.75 AGMFFSFAGDD31 pKa = 3.45 EE32 pKa = 4.32 VNITDD37 pKa = 3.77 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.824
IPC2_protein 3.77
IPC_protein 3.706
Toseland 3.49
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.719
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.834
Patrickios 0.223
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A1L2JXQ8|A0A1L2JXQ8_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC926 OX=1913082 GN=CHPC926_0014 PE=4 SV=1
MM1 pKa = 7.56 SKK3 pKa = 9.22 TKK5 pKa = 10.5 RR6 pKa = 11.84 GVCANCHH13 pKa = 4.61 TVFEE17 pKa = 4.53 VSKK20 pKa = 10.01 KK21 pKa = 6.75 QRR23 pKa = 11.84 RR24 pKa = 11.84 KK25 pKa = 8.8 IKK27 pKa = 8.88 EE28 pKa = 4.09 GKK30 pKa = 9.71 SVFCSEE36 pKa = 4.16 TCSLEE41 pKa = 4.41 KK42 pKa = 10.69 YY43 pKa = 9.93 GKK45 pKa = 8.13 TKK47 pKa = 9.25 ITISEE52 pKa = 4.09 IPP54 pKa = 3.22
Molecular weight: 6.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.942
IPC2_protein 8.916
IPC_protein 8.843
Toseland 9.94
ProMoST 9.443
Dawson 10.043
Bjellqvist 9.692
Wikipedia 10.116
Rodwell 10.804
Grimsley 10.043
Solomon 10.087
Lehninger 10.087
Nozaki 10.058
DTASelect 9.633
Thurlkill 9.94
EMBOSS 10.306
Sillero 9.999
Patrickios 10.643
IPC_peptide 10.101
IPC2_peptide 8.478
IPC2.peptide.svr19 7.857
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
8861
46
910
201.4
22.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.489 ± 0.42
0.756 ± 0.12
6.083 ± 0.257
7.46 ± 0.645
4.435 ± 0.227
6.196 ± 0.565
1.174 ± 0.127
6.85 ± 0.476
8.961 ± 0.521
7.877 ± 0.407
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.168
6.489 ± 0.568
2.562 ± 0.162
4.334 ± 0.261
3.814 ± 0.332
6.376 ± 0.337
6.5 ± 0.256
6.004 ± 0.27
1.219 ± 0.113
3.95 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here