Paenibacillus phage Halcyone

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Halcyonevirus; Paenibacillus virus Halcyone

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AW19|A0A345AW19_9CAUD Uncharacterized protein OS=Paenibacillus phage Halcyone OX=1636256 GN=HALCYONE_58 PE=4 SV=1
MM1 pKa = 7.95PEE3 pKa = 4.26LDD5 pKa = 4.59RR6 pKa = 11.84FNPDD10 pKa = 2.95DD11 pKa = 3.98TDD13 pKa = 5.19PIVASCASCDD23 pKa = 3.55GEE25 pKa = 4.35IYY27 pKa = 10.73EE28 pKa = 4.75GDD30 pKa = 3.87SVVLTTEE37 pKa = 3.87GDD39 pKa = 3.98FVHH42 pKa = 7.34DD43 pKa = 3.45EE44 pKa = 4.22CFAAFARR51 pKa = 11.84EE52 pKa = 4.36TYY54 pKa = 10.1RR55 pKa = 11.84SASGTIDD62 pKa = 3.22ANGRR66 pKa = 11.84III68 pKa = 4.67

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AVZ1|A0A345AVZ1_9CAUD Uncharacterized protein OS=Paenibacillus phage Halcyone OX=1636256 GN=HALCYONE_28 PE=4 SV=1
MM1 pKa = 7.62ALLTRR6 pKa = 11.84TCRR9 pKa = 11.84QCQASFQGGPRR20 pKa = 11.84AYY22 pKa = 10.46YY23 pKa = 10.59CPTCRR28 pKa = 11.84AEE30 pKa = 4.3RR31 pKa = 11.84TRR33 pKa = 11.84KK34 pKa = 8.1TYY36 pKa = 11.11AEE38 pKa = 3.85YY39 pKa = 10.6KK40 pKa = 8.75RR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 8.56RR44 pKa = 11.84QGKK47 pKa = 9.03ARR49 pKa = 11.84ALGSTDD55 pKa = 2.76TCEE58 pKa = 4.22RR59 pKa = 11.84CGKK62 pKa = 10.24SYY64 pKa = 11.01VVNAGLQRR72 pKa = 11.84FCPDD76 pKa = 3.62CQPIHH81 pKa = 6.4AAEE84 pKa = 4.18HH85 pKa = 6.35DD86 pKa = 3.65RR87 pKa = 11.84RR88 pKa = 11.84TSLSYY93 pKa = 11.53YY94 pKa = 9.77NANKK98 pKa = 9.04DD99 pKa = 3.83TINPARR105 pKa = 11.84NQKK108 pKa = 9.03RR109 pKa = 11.84RR110 pKa = 11.84EE111 pKa = 3.35WRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84KK116 pKa = 9.24KK117 pKa = 10.65AKK119 pKa = 9.9NAPQQQ124 pKa = 3.26

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

17127

31

964

190.3

21.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.057 ± 0.314

1.039 ± 0.108

6.878 ± 0.195

8.151 ± 0.294

3.556 ± 0.185

6.545 ± 0.29

1.699 ± 0.106

6.224 ± 0.166

7.082 ± 0.316

8.262 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.522 ± 0.146

3.772 ± 0.148

3.509 ± 0.184

2.89 ± 0.194

6.633 ± 0.291

5.267 ± 0.161

5.494 ± 0.178

6.755 ± 0.231

1.466 ± 0.127

4.192 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski