Escherichia phage Penshu1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NAG1|A0A5B9NAG1_9CAUD Internal virion protein gp15 OS=Escherichia phage Penshu1 OX=2591101 GN=CPT_Penshu1_044 PE=3 SV=1
MM1 pKa = 6.87SQSVYY6 pKa = 11.38AEE8 pKa = 4.16FGVSSNAVTGSVEE21 pKa = 5.06DD22 pKa = 4.16LNEE25 pKa = 4.16HH26 pKa = 5.47QKK28 pKa = 11.61SMLEE32 pKa = 3.55QDD34 pKa = 3.61VAVRR38 pKa = 11.84DD39 pKa = 3.75GDD41 pKa = 3.85DD42 pKa = 4.58AITFKK47 pKa = 10.85QLEE50 pKa = 4.43AEE52 pKa = 4.22SEE54 pKa = 4.16EE55 pKa = 4.02ATEE58 pKa = 3.71EE59 pKa = 4.17DD60 pKa = 4.03EE61 pKa = 5.06NVEE64 pKa = 4.05EE65 pKa = 4.5TEE67 pKa = 4.0GEE69 pKa = 4.0EE70 pKa = 4.22DD71 pKa = 4.13HH72 pKa = 7.29EE73 pKa = 5.84SDD75 pKa = 5.18DD76 pKa = 4.98EE77 pKa = 4.22EE78 pKa = 5.98SEE80 pKa = 4.31TDD82 pKa = 3.5GEE84 pKa = 4.22QPEE87 pKa = 4.71FIEE90 pKa = 5.95LGDD93 pKa = 3.86APKK96 pKa = 10.67EE97 pKa = 3.96LTEE100 pKa = 4.52SVTALDD106 pKa = 3.81EE107 pKa = 4.32NEE109 pKa = 4.15AAFDD113 pKa = 4.13DD114 pKa = 4.55MVSSAVEE121 pKa = 3.96AGKK124 pKa = 8.43VTADD128 pKa = 3.95EE129 pKa = 4.11ITSIKK134 pKa = 10.63AEE136 pKa = 3.97YY137 pKa = 10.49AKK139 pKa = 10.65DD140 pKa = 3.61GKK142 pKa = 11.09LSDD145 pKa = 3.33ASYY148 pKa = 11.61AKK150 pKa = 10.33LQEE153 pKa = 3.93AGYY156 pKa = 7.75TKK158 pKa = 10.52RR159 pKa = 11.84FVDD162 pKa = 3.55SFVRR166 pKa = 11.84GQEE169 pKa = 3.91ALAEE173 pKa = 4.09QYY175 pKa = 10.62AAGVVRR181 pKa = 11.84YY182 pKa = 9.78AGGAEE187 pKa = 3.89QFNRR191 pKa = 11.84ILSHH195 pKa = 6.95LEE197 pKa = 3.78SNDD200 pKa = 2.97PSTRR204 pKa = 11.84EE205 pKa = 3.83ALEE208 pKa = 3.76AAIVRR213 pKa = 11.84KK214 pKa = 10.32DD215 pKa = 3.1IATTKK220 pKa = 10.96ALLNLAGKK228 pKa = 7.93TLGKK232 pKa = 10.41AVGVKK237 pKa = 8.57PQRR240 pKa = 11.84TITAQAKK247 pKa = 9.26PVVAPKK253 pKa = 10.58APQTEE258 pKa = 4.38AFSSKK263 pKa = 10.93ADD265 pKa = 3.78MIKK268 pKa = 10.77AMSDD272 pKa = 2.96PRR274 pKa = 11.84YY275 pKa = 10.34LRR277 pKa = 11.84DD278 pKa = 3.04AKK280 pKa = 10.36YY281 pKa = 9.52TMEE284 pKa = 3.95VRR286 pKa = 11.84AKK288 pKa = 10.22VAASSLL294 pKa = 3.78

Molecular weight: 31.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N6C4|A0A5B9N6C4_9CAUD SsDNA binding protein OS=Escherichia phage Penshu1 OX=2591101 GN=CPT_Penshu1_016 PE=4 SV=1
MM1 pKa = 7.73VDD3 pKa = 3.64EE4 pKa = 5.21RR5 pKa = 11.84SSTDD9 pKa = 2.49RR10 pKa = 11.84WPQRR14 pKa = 11.84LPLTLIGVNYY24 pKa = 7.29EE25 pKa = 3.8HH26 pKa = 7.63RR27 pKa = 11.84LLGMLISGNLSDD39 pKa = 5.06SLGASGRR46 pKa = 11.84NQARR50 pKa = 11.84VILMSWILPVIFGYY64 pKa = 10.85GLIAYY69 pKa = 9.38VMGRR73 pKa = 11.84DD74 pKa = 3.14INKK77 pKa = 9.24ARR79 pKa = 11.84KK80 pKa = 8.78VYY82 pKa = 9.84KK83 pKa = 10.44LNYY86 pKa = 8.5VRR88 pKa = 11.84LGRR91 pKa = 11.84WTVRR95 pKa = 11.84QPNGRR100 pKa = 11.84FMRR103 pKa = 11.84NLANVWDD110 pKa = 4.02IATLGSKK117 pKa = 10.07LL118 pKa = 3.58

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12641

30

1296

234.1

26.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.583 ± 0.437

1.068 ± 0.235

6.408 ± 0.264

6.503 ± 0.34

3.52 ± 0.183

8.006 ± 0.385

1.827 ± 0.198

4.778 ± 0.152

6.685 ± 0.345

8.196 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.864 ± 0.158

4.383 ± 0.224

3.789 ± 0.165

3.742 ± 0.367

5.506 ± 0.163

6.329 ± 0.325

5.807 ± 0.244

7.017 ± 0.261

1.456 ± 0.171

3.536 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski