Serratia symbiotica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Serratia

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068Z5C4|A0A068Z5C4_9GAMM Two-component system response regulator GlrR OS=Serratia symbiotica OX=138074 GN=glrR PE=4 SV=1
MM1 pKa = 7.69SLEE4 pKa = 4.15NASPTLQLAVDD15 pKa = 5.13LIYY18 pKa = 10.95LLEE21 pKa = 4.65CNEE24 pKa = 4.16IDD26 pKa = 3.92PATVLAALDD35 pKa = 3.84IVKK38 pKa = 10.0QDD40 pKa = 3.66YY41 pKa = 9.31QEE43 pKa = 4.62KK44 pKa = 9.78LQHH47 pKa = 6.29TNTNSPYY54 pKa = 10.41LAYY57 pKa = 10.12EE58 pKa = 4.35LDD60 pKa = 4.17DD61 pKa = 5.1DD62 pKa = 4.87RR63 pKa = 11.84LPP65 pKa = 4.14

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068Z6B7|A0A068Z6B7_9GAMM Pseudouridine synthase OS=Serratia symbiotica OX=138074 GN=rluF PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MANKK25 pKa = 9.98NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2840

20

2860

861842

14

5398

301.3

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.519 ± 0.062

1.13 ± 0.017

5.207 ± 0.033

5.522 ± 0.047

3.605 ± 0.031

7.235 ± 0.041

2.368 ± 0.023

5.727 ± 0.047

4.348 ± 0.047

11.066 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.023

3.832 ± 0.037

4.402 ± 0.035

4.881 ± 0.048

5.965 ± 0.041

6.106 ± 0.039

5.254 ± 0.038

6.926 ± 0.042

1.391 ± 0.022

2.871 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski