Pacific flying fox faeces associated gemycircularvirus-2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus ptero2; Pteropus associated gemycircularvirus 2

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTL3|A0A140CTL3_9VIRU RepA OS=Pacific flying fox faeces associated gemycircularvirus-2 OX=1795994 PE=3 SV=1
MM1 pKa = 7.52SFYY4 pKa = 10.7FCARR8 pKa = 11.84YY9 pKa = 9.94GLFTYY14 pKa = 7.73SQCADD19 pKa = 3.59LNHH22 pKa = 6.75WSVLDD27 pKa = 3.89LFSGLGAEE35 pKa = 4.62CIIGRR40 pKa = 11.84EE41 pKa = 4.06LHH43 pKa = 6.69EE44 pKa = 5.62DD45 pKa = 3.63GGTHH49 pKa = 6.0LHH51 pKa = 5.94VFADD55 pKa = 4.51FGRR58 pKa = 11.84RR59 pKa = 11.84FRR61 pKa = 11.84SRR63 pKa = 11.84SSKK66 pKa = 10.5IFDD69 pKa = 3.52CEE71 pKa = 3.87GRR73 pKa = 11.84HH74 pKa = 5.88PNVSASRR81 pKa = 11.84GKK83 pKa = 9.64PDD85 pKa = 3.84EE86 pKa = 4.41GWDD89 pKa = 3.65YY90 pKa = 11.6AVKK93 pKa = 10.85DD94 pKa = 3.75GDD96 pKa = 4.04VVAGGLEE103 pKa = 4.06RR104 pKa = 11.84PDD106 pKa = 3.18GVTRR110 pKa = 11.84RR111 pKa = 11.84SGDD114 pKa = 3.41STSDD118 pKa = 3.5QKK120 pKa = 10.77WGEE123 pKa = 3.71IAGAEE128 pKa = 4.23DD129 pKa = 4.33RR130 pKa = 11.84DD131 pKa = 4.16EE132 pKa = 4.4FWRR135 pKa = 11.84LVHH138 pKa = 6.6EE139 pKa = 5.37LDD141 pKa = 3.76PKK143 pKa = 10.92SLVIHH148 pKa = 6.44FPAISKK154 pKa = 9.21YY155 pKa = 10.09CDD157 pKa = 2.66WKK159 pKa = 10.33FSPRR163 pKa = 11.84QVEE166 pKa = 4.27YY167 pKa = 10.58VHH169 pKa = 7.13PINIDD174 pKa = 3.59FVGGEE179 pKa = 4.07VDD181 pKa = 6.26GRR183 pKa = 11.84DD184 pKa = 3.02HH185 pKa = 7.12WLAQAGIGLGGTGKK199 pKa = 10.08SPQMNTWGPRR209 pKa = 11.84FHH211 pKa = 7.18PVGLRR216 pKa = 11.84GMKK219 pKa = 9.75SLCVYY224 pKa = 10.15GKK226 pKa = 10.4SRR228 pKa = 11.84TGKK231 pKa = 7.21TLWARR236 pKa = 11.84SLGRR240 pKa = 11.84HH241 pKa = 6.26IYY243 pKa = 10.61CIGLVSGAEE252 pKa = 4.07CARR255 pKa = 11.84AEE257 pKa = 4.24EE258 pKa = 3.7VDD260 pKa = 3.82YY261 pKa = 11.52AVFDD265 pKa = 5.17DD266 pKa = 3.64IRR268 pKa = 11.84GGIKK272 pKa = 10.04FFHH275 pKa = 7.18AYY277 pKa = 8.84KK278 pKa = 10.42EE279 pKa = 4.32WMGAQAMVSVKK290 pKa = 10.42LLYY293 pKa = 10.06RR294 pKa = 11.84DD295 pKa = 3.67PKK297 pKa = 9.71LVRR300 pKa = 11.84WGKK303 pKa = 9.42PSIWLSNKK311 pKa = 9.94DD312 pKa = 3.65PRR314 pKa = 11.84CDD316 pKa = 3.25MSQEE320 pKa = 3.98DD321 pKa = 4.7VEE323 pKa = 4.31WLEE326 pKa = 4.29DD327 pKa = 3.16NCIFVEE333 pKa = 4.32CNEE336 pKa = 4.45TIFRR340 pKa = 11.84ANTEE344 pKa = 3.92

Molecular weight: 38.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTL2|A0A140CTL2_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated gemycircularvirus-2 OX=1795994 PE=3 SV=1
MM1 pKa = 7.86AYY3 pKa = 9.52PRR5 pKa = 11.84RR6 pKa = 11.84SYY8 pKa = 10.78RR9 pKa = 11.84RR10 pKa = 11.84ASGRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84VSRR19 pKa = 11.84RR20 pKa = 11.84APRR23 pKa = 11.84RR24 pKa = 11.84TKK26 pKa = 8.32RR27 pKa = 11.84TYY29 pKa = 9.12RR30 pKa = 11.84RR31 pKa = 11.84TYY33 pKa = 8.42RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84VGGRR41 pKa = 11.84GGRR44 pKa = 11.84RR45 pKa = 11.84SLLNATSRR53 pKa = 11.84KK54 pKa = 9.16KK55 pKa = 10.63RR56 pKa = 11.84NTMLQFVNTNSTSGLPVTPGPGPYY80 pKa = 9.61VVAGNQGSAFSVFTATAMDD99 pKa = 5.05LYY101 pKa = 11.49ANGNANTIANQAQRR115 pKa = 11.84TSTTCYY121 pKa = 9.27VLGYY125 pKa = 10.01AEE127 pKa = 5.45KK128 pKa = 10.47IRR130 pKa = 11.84IQTSSGLPWFWRR142 pKa = 11.84RR143 pKa = 11.84IVVRR147 pKa = 11.84VRR149 pKa = 11.84DD150 pKa = 4.03PLFVTYY156 pKa = 8.58ATNDD160 pKa = 3.38TPTYY164 pKa = 8.14SANMSYY170 pKa = 11.39VDD172 pKa = 3.42TTTNGMQRR180 pKa = 11.84AYY182 pKa = 9.98INQTINAANNTITGVQGVLFRR203 pKa = 11.84GTVGRR208 pKa = 11.84DD209 pKa = 2.66WTDD212 pKa = 2.53IQTAIVDD219 pKa = 3.85TTRR222 pKa = 11.84VDD224 pKa = 3.72LVSDD228 pKa = 3.17TRR230 pKa = 11.84MTIRR234 pKa = 11.84SGNANGTVKK243 pKa = 10.38DD244 pKa = 3.68VNMWHH249 pKa = 7.53PYY251 pKa = 9.03RR252 pKa = 11.84RR253 pKa = 11.84NLVYY257 pKa = 10.77DD258 pKa = 4.09DD259 pKa = 5.63DD260 pKa = 4.35EE261 pKa = 6.47LGEE264 pKa = 4.25QEE266 pKa = 4.07TANYY270 pKa = 10.15VSTEE274 pKa = 4.03SKK276 pKa = 10.5QGGGDD281 pKa = 3.18MHH283 pKa = 8.32VIDD286 pKa = 5.07IFTPGTGAGASDD298 pKa = 4.5LLQLTSTSTLYY309 pKa = 9.69WHH311 pKa = 7.1EE312 pKa = 4.2KK313 pKa = 9.32

Molecular weight: 35.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

896

239

344

298.7

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 0.559

2.009 ± 0.79

6.92 ± 0.829

4.464 ± 1.208

4.353 ± 0.98

10.491 ± 0.946

2.79 ± 0.854

4.353 ± 0.238

3.571 ± 0.71

6.027 ± 0.43

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.786 ± 0.408

3.683 ± 1.394

3.906 ± 0.258

2.679 ± 0.53

9.152 ± 1.227

6.92 ± 0.101

6.696 ± 2.803

7.478 ± 0.39

2.79 ± 0.548

3.683 ± 0.673
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski