Sporomusa malonica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Sporomusa

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W2EJK7|A0A1W2EJK7_9FIRM Flagellin OS=Sporomusa malonica OX=112901 GN=SAMN04488500_12513 PE=3 SV=1
MM1 pKa = 7.74ASTFAGLSITSRR13 pKa = 11.84GLNAAQVGLTVTTNNMSNIDD33 pKa = 3.34TDD35 pKa = 4.26GYY37 pKa = 10.12SRR39 pKa = 11.84QVVSQTSIGPAAVYY53 pKa = 10.45SSSLVGAGVEE63 pKa = 4.29VTSVDD68 pKa = 3.14RR69 pKa = 11.84VRR71 pKa = 11.84SFRR74 pKa = 11.84LDD76 pKa = 3.19QKK78 pKa = 10.67YY79 pKa = 8.1WQTNSAASEE88 pKa = 4.41LEE90 pKa = 3.8AQSYY94 pKa = 8.43YY95 pKa = 10.8LEE97 pKa = 4.77SIEE100 pKa = 4.48EE101 pKa = 4.39VFGSTSTSDD110 pKa = 2.45ITTDD114 pKa = 3.43LNTFATDD121 pKa = 4.14LEE123 pKa = 4.59TLATDD128 pKa = 3.83PTNEE132 pKa = 4.09STRR135 pKa = 11.84ATVLADD141 pKa = 3.38AEE143 pKa = 4.93TFCEE147 pKa = 4.38TLNSAADD154 pKa = 3.84EE155 pKa = 4.24LSQIRR160 pKa = 11.84SDD162 pKa = 3.58INSDD166 pKa = 3.37VYY168 pKa = 9.11TTVEE172 pKa = 4.09QINSYY177 pKa = 10.81AEE179 pKa = 3.74QIAALNKK186 pKa = 9.54QISVATASDD195 pKa = 3.35ASTNDD200 pKa = 4.64LEE202 pKa = 4.62DD203 pKa = 3.65QQDD206 pKa = 4.01LLVDD210 pKa = 3.99EE211 pKa = 5.06LSGLIGIGVTEE222 pKa = 4.2TDD224 pKa = 3.03KK225 pKa = 11.76GEE227 pKa = 3.94YY228 pKa = 9.34TITVDD233 pKa = 4.5GVALVSGDD241 pKa = 3.67DD242 pKa = 3.8ANEE245 pKa = 4.1LEE247 pKa = 5.33CYY249 pKa = 8.99TVTDD253 pKa = 3.96ATSDD257 pKa = 3.31QYY259 pKa = 12.12GMYY262 pKa = 10.07GIRR265 pKa = 11.84WAEE268 pKa = 3.93SGEE271 pKa = 4.22DD272 pKa = 3.93FDD274 pKa = 5.29TGDD277 pKa = 3.45SGALNGYY284 pKa = 9.22LAVRR288 pKa = 11.84DD289 pKa = 4.19GNSSEE294 pKa = 4.22NKK296 pKa = 9.36GVVYY300 pKa = 9.68YY301 pKa = 10.96SNQLDD306 pKa = 3.52EE307 pKa = 4.69FARR310 pKa = 11.84TFAEE314 pKa = 4.09AFNEE318 pKa = 4.47GVTSGTTTYY327 pKa = 10.59NGHH330 pKa = 6.84ADD332 pKa = 3.39GVGIDD337 pKa = 3.95DD338 pKa = 3.8TTDD341 pKa = 2.65IRR343 pKa = 11.84FFTYY347 pKa = 10.65DD348 pKa = 3.6DD349 pKa = 4.31LSSEE353 pKa = 4.37EE354 pKa = 4.35LMASGTDD361 pKa = 3.09MDD363 pKa = 5.21EE364 pKa = 4.22IYY366 pKa = 11.47SNITAANISVSKK378 pKa = 10.44DD379 pKa = 3.05IQEE382 pKa = 4.51DD383 pKa = 3.62TDD385 pKa = 4.45KK386 pKa = 11.13IATASSDD393 pKa = 3.72GEE395 pKa = 4.22EE396 pKa = 4.36GNSEE400 pKa = 4.36NIDD403 pKa = 4.38DD404 pKa = 5.19IISICSDD411 pKa = 3.34VNVSGNATITDD422 pKa = 4.78LYY424 pKa = 10.21NVIVATVAGDD434 pKa = 3.37SGSAKK439 pKa = 9.91NAYY442 pKa = 7.53EE443 pKa = 4.01RR444 pKa = 11.84KK445 pKa = 9.47SANATYY451 pKa = 10.29IDD453 pKa = 3.76TSRR456 pKa = 11.84SSVSGVSSDD465 pKa = 3.68EE466 pKa = 3.92EE467 pKa = 4.73TVNLTVYY474 pKa = 10.16QSAYY478 pKa = 9.75AASASMASAWNEE490 pKa = 3.91VYY492 pKa = 9.45DD493 pKa = 4.61TILAMVDD500 pKa = 3.48DD501 pKa = 4.67

Molecular weight: 53.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W2D3D1|A0A1W2D3D1_9FIRM Cysteine desulfurase family protein OS=Sporomusa malonica OX=112901 GN=SAMN04488500_11341 PE=3 SV=1
MM1 pKa = 6.67QRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84SKK7 pKa = 11.0HH8 pKa = 4.05FVKK11 pKa = 10.77LSGKK15 pKa = 8.57RR16 pKa = 11.84TVTGLYY22 pKa = 9.75SGEE25 pKa = 4.32HH26 pKa = 5.48IRR28 pKa = 11.84LFADD32 pKa = 3.4VTDD35 pKa = 4.74GEE37 pKa = 4.39PCRR40 pKa = 11.84GKK42 pKa = 9.78SLRR45 pKa = 11.84YY46 pKa = 8.52NGQRR50 pKa = 11.84QRR52 pKa = 11.84EE53 pKa = 4.37LFRR56 pKa = 11.84FASVFYY62 pKa = 9.76FVHH65 pKa = 6.95FLACMLAAGG74 pKa = 4.35

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4926

0

4926

1505841

39

4427

305.7

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.19 ± 0.046

1.237 ± 0.014

4.956 ± 0.027

6.158 ± 0.037

3.8 ± 0.026

7.617 ± 0.043

1.844 ± 0.015

7.473 ± 0.028

5.798 ± 0.033

9.73 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.017

4.122 ± 0.035

3.902 ± 0.024

3.794 ± 0.024

4.487 ± 0.033

5.723 ± 0.028

5.634 ± 0.038

7.565 ± 0.024

0.963 ± 0.014

3.23 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski