Vibrio phage pYD21-A
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4QC66|M4QC66_9CAUD Uncharacterized protein OS=Vibrio phage pYD21-A OX=754049 GN=VPKG_00072 PE=4 SV=1
MM1 pKa = 7.58 DD2 pKa = 4.64 WSWIDD7 pKa = 3.24 MTATTEE13 pKa = 3.76 PKK15 pKa = 10.12 YY16 pKa = 10.83 FVIGPVLEE24 pKa = 4.79 TINNAMLDD32 pKa = 3.81 EE33 pKa = 4.62 MEE35 pKa = 4.67 PPYY38 pKa = 10.33 KK39 pKa = 9.6 IGMSSSVKK47 pKa = 9.83 EE48 pKa = 4.29 LYY50 pKa = 10.45 DD51 pKa = 4.32 SEE53 pKa = 4.63 VEE55 pKa = 4.41 QMKK58 pKa = 10.25 HH59 pKa = 6.19 DD60 pKa = 3.97 KK61 pKa = 11.44 AFMQCLGYY69 pKa = 10.7 VDD71 pKa = 5.4 IIVDD75 pKa = 4.87 DD76 pKa = 4.55 EE77 pKa = 4.54 LDD79 pKa = 3.5 SDD81 pKa = 4.75 GVYY84 pKa = 11.09 LLAA87 pKa = 6.46
Molecular weight: 9.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.656
EMBOSS 3.732
Sillero 3.91
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|M4QC60|M4QC60_9CAUD Uncharacterized protein OS=Vibrio phage pYD21-A OX=754049 GN=VPKG_00067 PE=4 SV=1
MM1 pKa = 6.55 QTQNNVRR8 pKa = 11.84 TTNIEE13 pKa = 3.72 VATIKK18 pKa = 10.86 DD19 pKa = 3.34 AMAFNYY25 pKa = 9.95 GNQRR29 pKa = 11.84 GMAKK33 pKa = 10.16 HH34 pKa = 6.33 LGINRR39 pKa = 11.84 GTLAKK44 pKa = 10.24 KK45 pKa = 9.78 LASNRR50 pKa = 11.84 DD51 pKa = 3.39 YY52 pKa = 10.79 YY53 pKa = 11.51 VRR55 pKa = 11.84 VHH57 pKa = 6.92 RR58 pKa = 11.84 DD59 pKa = 2.36 GWNNITHH66 pKa = 6.91 LEE68 pKa = 4.11 WLNKK72 pKa = 9.64
Molecular weight: 8.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.253
IPC2_protein 9.677
IPC_protein 10.072
Toseland 10.379
ProMoST 10.131
Dawson 10.54
Bjellqvist 10.204
Wikipedia 10.716
Rodwell 10.935
Grimsley 10.599
Solomon 10.584
Lehninger 10.555
Nozaki 10.335
DTASelect 10.204
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.452
Patrickios 10.716
IPC_peptide 10.584
IPC2_peptide 8.843
IPC2.peptide.svr19 8.726
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
14180
49
1077
191.6
21.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.173 ± 0.446
1.403 ± 0.173
6.312 ± 0.234
7.073 ± 0.316
3.568 ± 0.137
7.32 ± 0.286
2.052 ± 0.242
5.994 ± 0.212
6.354 ± 0.403
7.292 ± 0.195
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.856 ± 0.231
5.176 ± 0.26
3.66 ± 0.181
3.505 ± 0.162
4.669 ± 0.21
6.608 ± 0.232
6.058 ± 0.319
6.7 ± 0.237
1.382 ± 0.1
3.843 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here