Actinobacillus seminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Actinobacillu

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A263HE26|A0A263HE26_9PAST Exodeoxyribonuclease 7 large subunit OS=Actinobacillus seminis OX=722 GN=xseA PE=3 SV=1
MM1 pKa = 7.28YY2 pKa = 8.59PQLYY6 pKa = 9.27IDD8 pKa = 4.93GVSEE12 pKa = 5.38DD13 pKa = 3.46LAGWEE18 pKa = 4.21EE19 pKa = 4.09ILFHH23 pKa = 6.47FNVSVEE29 pKa = 4.26DD30 pKa = 3.81NEE32 pKa = 4.09VWAVARR38 pKa = 11.84CCEE41 pKa = 4.4EE42 pKa = 3.77IPHH45 pKa = 7.04LGNIYY50 pKa = 10.22QSLVLDD56 pKa = 3.82RR57 pKa = 11.84LEE59 pKa = 4.36SLFFEE64 pKa = 4.84QLDD67 pKa = 3.76LDD69 pKa = 4.48EE70 pKa = 7.01DD71 pKa = 4.33DD72 pKa = 3.92EE73 pKa = 6.72HH74 pKa = 8.84IDD76 pKa = 3.33VSTFVNDD83 pKa = 3.97LDD85 pKa = 4.05SHH87 pKa = 5.88FCIDD91 pKa = 3.62GDD93 pKa = 5.06AITTLDD99 pKa = 3.22EE100 pKa = 4.49FMAKK104 pKa = 9.78VEE106 pKa = 4.39EE107 pKa = 4.43IKK109 pKa = 10.98SILHH113 pKa = 5.92

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A263HDC9|A0A263HDC9_9PAST RNA polymerase sigma factor OS=Actinobacillus seminis OX=722 GN=rpoE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2028

0

2028

634438

21

5396

312.8

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.586 ± 0.061

1.027 ± 0.023

5.078 ± 0.054

6.067 ± 0.056

4.261 ± 0.054

6.892 ± 0.084

2.083 ± 0.03

6.942 ± 0.05

6.173 ± 0.078

10.387 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.036

4.816 ± 0.068

3.68 ± 0.04

4.915 ± 0.067

4.524 ± 0.054

5.814 ± 0.049

5.297 ± 0.067

6.749 ± 0.044

1.149 ± 0.026

3.187 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski