Arachis hypogaea (Peanut)
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 97596 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A445E6T8|A0A445E6T8_ARAHY Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A02g005435 PE=4 SV=1
MM1 pKa = 7.77 DD2 pKa = 3.54 VKK4 pKa = 11.08 GNNVTPDD11 pKa = 3.76 FSSYY15 pKa = 11.15 ILFEE19 pKa = 4.3 ASGDD23 pKa = 3.89 SEE25 pKa = 4.12 EE26 pKa = 6.15 ADD28 pKa = 3.75 FNHH31 pKa = 6.24 NKK33 pKa = 8.43 MVCEE37 pKa = 4.36 ISRR40 pKa = 11.84 VGSYY44 pKa = 7.33 VHH46 pKa = 6.96 HH47 pKa = 6.22 GHH49 pKa = 6.84 EE50 pKa = 5.63 DD51 pKa = 3.52 YY52 pKa = 11.55 DD53 pKa = 5.52 DD54 pKa = 6.14 DD55 pKa = 7.24 DD56 pKa = 5.42 DD57 pKa = 6.0 ALSCSYY63 pKa = 10.96 DD64 pKa = 3.59 RR65 pKa = 11.84 SDD67 pKa = 5.13 YY68 pKa = 11.59 DD69 pKa = 5.26 DD70 pKa = 4.85 DD71 pKa = 4.18 ACNVAEE77 pKa = 4.81 VNWDD81 pKa = 4.18 DD82 pKa = 6.48 DD83 pKa = 5.97 DD84 pKa = 7.68 DD85 pKa = 7.44 DD86 pKa = 7.49 DD87 pKa = 7.49 DD88 pKa = 7.06 DD89 pKa = 5.62 GEE91 pKa = 4.48 KK92 pKa = 10.44 KK93 pKa = 10.58 DD94 pKa = 4.64 DD95 pKa = 3.73 VFGTSYY101 pKa = 10.93 CDD103 pKa = 3.56 EE104 pKa = 4.45 EE105 pKa = 6.15 DD106 pKa = 3.67 EE107 pKa = 4.75 VLQEE111 pKa = 3.83 QHH113 pKa = 5.93 PTRR116 pKa = 11.84 SFVSFASNQEE126 pKa = 4.17 SLDD129 pKa = 3.6 EE130 pKa = 4.11 MEE132 pKa = 4.68 KK133 pKa = 10.72 NRR135 pKa = 11.84 LFWEE139 pKa = 4.55 ACLASS144 pKa = 3.65
Molecular weight: 16.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.376
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.075
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.821
Patrickios 1.163
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A445CE00|A0A445CE00_ARAHY Isoform of A0A445CDQ1 Protein FAR1-RELATED SEQUENCE OS=Arachis hypogaea OX=3818 GN=Ahy_A07g035374 PE=3 SV=1
MM1 pKa = 7.53 LVTLQRR7 pKa = 11.84 RR8 pKa = 11.84 QGALQRR14 pKa = 11.84 MLGSLQRR21 pKa = 11.84 KK22 pKa = 9.15 LGALQRR28 pKa = 11.84 WLGALQRR35 pKa = 11.84 RR36 pKa = 11.84 QVALQRR42 pKa = 11.84 MLGSLQRR49 pKa = 11.84 MLRR52 pKa = 11.84 ALQRR56 pKa = 11.84 LLGALQRR63 pKa = 11.84 RR64 pKa = 11.84 QVALQRR70 pKa = 11.84 RR71 pKa = 11.84 QGASQRR77 pKa = 11.84 WLGVLQRR84 pKa = 11.84 WLGALQRR91 pKa = 11.84 RR92 pKa = 11.84 QVALQRR98 pKa = 11.84 WLRR101 pKa = 11.84 AVQRR105 pKa = 11.84 MLGALQRR112 pKa = 11.84 WLGALQRR119 pKa = 11.84 WLEE122 pKa = 3.99 ALQRR126 pKa = 11.84 RR127 pKa = 11.84 QGALQRR133 pKa = 11.84 RR134 pKa = 11.84 QGALQRR140 pKa = 11.84 WPVTLQRR147 pKa = 11.84 KK148 pKa = 8.14 QVDD151 pKa = 4.12 LQRR154 pKa = 11.84 MLGSLQRR161 pKa = 11.84 KK162 pKa = 9.15 LGALQRR168 pKa = 11.84 WLGALQRR175 pKa = 11.84 MLSALQRR182 pKa = 11.84 WLVTLQRR189 pKa = 11.84 RR190 pKa = 11.84 QGALEE195 pKa = 4.03 RR196 pKa = 11.84 MLVTT200 pKa = 4.93
Molecular weight: 23.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.155
IPC_protein 12.764
Toseland 12.925
ProMoST 13.437
Dawson 12.925
Bjellqvist 12.925
Wikipedia 13.408
Rodwell 12.471
Grimsley 12.969
Solomon 13.437
Lehninger 13.334
Nozaki 12.925
DTASelect 12.925
Thurlkill 12.925
EMBOSS 13.437
Sillero 12.925
Patrickios 12.179
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.189
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71122
26474
97596
37456558
8
5625
383.8
43.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.486 ± 0.008
1.951 ± 0.004
5.283 ± 0.005
6.489 ± 0.008
4.304 ± 0.005
6.174 ± 0.007
2.615 ± 0.003
5.451 ± 0.006
6.154 ± 0.008
9.549 ± 0.01
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.49 ± 0.003
4.681 ± 0.006
4.853 ± 0.008
3.718 ± 0.005
5.433 ± 0.007
8.709 ± 0.009
4.939 ± 0.005
6.476 ± 0.007
1.352 ± 0.003
2.892 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here