Streptococcus satellite phage Javan303
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKZ8|A0A4D5ZKZ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan303 OX=2558623 GN=JavanS303_0010 PE=4 SV=1
MM1 pKa = 7.34 TLDD4 pKa = 4.35 LDD6 pKa = 3.64 NMTRR10 pKa = 11.84 SEE12 pKa = 4.17 FDD14 pKa = 3.12 KK15 pKa = 11.38 LMTKK19 pKa = 10.08 IKK21 pKa = 10.49 DD22 pKa = 3.47 RR23 pKa = 11.84 NPNLFQFIIDD33 pKa = 3.88 FLDD36 pKa = 4.06 DD37 pKa = 3.54 KK38 pKa = 10.4 VTPEE42 pKa = 3.89 EE43 pKa = 4.34 VYY45 pKa = 11.05 DD46 pKa = 3.84 FLKK49 pKa = 10.18 MEE51 pKa = 4.85 RR52 pKa = 11.84 SYY54 pKa = 11.18 QVNYY58 pKa = 9.3 IKK60 pKa = 10.67 NYY62 pKa = 6.38 QARR65 pKa = 11.84 AA66 pKa = 3.37
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.94
IPC2_protein 5.054
IPC_protein 4.8
Toseland 4.622
ProMoST 4.94
Dawson 4.762
Bjellqvist 4.914
Wikipedia 4.685
Rodwell 4.635
Grimsley 4.533
Solomon 4.762
Lehninger 4.711
Nozaki 4.876
DTASelect 5.105
Thurlkill 4.647
EMBOSS 4.698
Sillero 4.914
Patrickios 4.266
IPC_peptide 4.762
IPC2_peptide 4.902
IPC2.peptide.svr19 4.967
Protein with the highest isoelectric point:
>tr|A0A4D5ZP68|A0A4D5ZP68_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan303 OX=2558623 GN=JavanS303_0011 PE=4 SV=1
MM1 pKa = 7.79 KK2 pKa = 10.19 IIQHH6 pKa = 5.09 TKK8 pKa = 8.83 KK9 pKa = 10.54 DD10 pKa = 3.26 GSAVYY15 pKa = 10.05 RR16 pKa = 11.84 SSIYY20 pKa = 10.71 LGIDD24 pKa = 2.85 SVTGKK29 pKa = 10.14 KK30 pKa = 10.39 VKK32 pKa = 8.21 TTISARR38 pKa = 11.84 TKK40 pKa = 10.75 KK41 pKa = 9.71 EE42 pKa = 4.04 LKK44 pKa = 10.64 NKK46 pKa = 7.95 ATQAKK51 pKa = 10.08 VEE53 pKa = 4.17 FEE55 pKa = 4.59 KK56 pKa = 11.24 NGSTRR61 pKa = 11.84 KK62 pKa = 9.17 QRR64 pKa = 11.84 SHH66 pKa = 5.33 ITTYY70 pKa = 11.06 SEE72 pKa = 4.48 LVDD75 pKa = 5.66 LFWQTYY81 pKa = 5.79 QHH83 pKa = 6.36 TIKK86 pKa = 10.07 TNTQIKK92 pKa = 10.31 IKK94 pKa = 10.56 GCLNNYY100 pKa = 8.27 LLPSFGTYY108 pKa = 10.67 KK109 pKa = 10.38 LDD111 pKa = 3.22 KK112 pKa = 8.85 LTPVIIQTQVNKK124 pKa = 9.76 WADD127 pKa = 3.8 EE128 pKa = 4.09 YY129 pKa = 11.47 NQDD132 pKa = 2.92 GTGYY136 pKa = 10.59 KK137 pKa = 10.0 EE138 pKa = 4.01 YY139 pKa = 11.38 NNLHH143 pKa = 6.45 ALNKK147 pKa = 10.23 RR148 pKa = 11.84 ILQYY152 pKa = 10.81 GVSIQALDD160 pKa = 3.67 NNPARR165 pKa = 11.84 DD166 pKa = 3.57 VVIPRR171 pKa = 11.84 KK172 pKa = 9.27 IARR175 pKa = 11.84 DD176 pKa = 3.47 KK177 pKa = 11.61 QEE179 pKa = 3.31 IKK181 pKa = 10.81 YY182 pKa = 9.97 FQDD185 pKa = 3.26 QEE187 pKa = 4.42 LKK189 pKa = 11.12 NFLSYY194 pKa = 11.28 LDD196 pKa = 3.81 NLEE199 pKa = 3.86 NTFINFYY206 pKa = 10.11 DD207 pKa = 3.61 TVLYY211 pKa = 8.15 KK212 pKa = 10.45 TLLATGLRR220 pKa = 11.84 IRR222 pKa = 11.84 EE223 pKa = 4.14 CLALEE228 pKa = 4.16 WSDD231 pKa = 4.72 IDD233 pKa = 4.27 LQNGTIDD240 pKa = 3.28 INKK243 pKa = 8.44 TLNILNQVNSPKK255 pKa = 9.85 TKK257 pKa = 9.48 SSYY260 pKa = 9.99 RR261 pKa = 11.84 VLDD264 pKa = 3.58 IDD266 pKa = 3.85 HH267 pKa = 6.69 KK268 pKa = 8.91 TVLMLRR274 pKa = 11.84 LYY276 pKa = 10.46 RR277 pKa = 11.84 ARR279 pKa = 11.84 QAEE282 pKa = 4.01 NGRR285 pKa = 11.84 NIGLTYY291 pKa = 10.56 EE292 pKa = 4.48 KK293 pKa = 10.64 VFSDD297 pKa = 4.51 SFDD300 pKa = 3.64 NYY302 pKa = 10.22 VNTRR306 pKa = 11.84 KK307 pKa = 9.81 VDD309 pKa = 3.68 YY310 pKa = 10.13 RR311 pKa = 11.84 LHH313 pKa = 6.14 KK314 pKa = 9.91 HH315 pKa = 6.17 LKK317 pKa = 9.07 NANCTDD323 pKa = 3.72 LGFHH327 pKa = 7.4 AFRR330 pKa = 11.84 HH331 pKa = 4.54 THH333 pKa = 6.71 ASILLNAGLPYY344 pKa = 10.43 KK345 pKa = 10.2 EE346 pKa = 3.68 IQTRR350 pKa = 11.84 LGHH353 pKa = 6.33 AKK355 pKa = 10.05 ISVTMDD361 pKa = 3.68 TYY363 pKa = 11.66 SHH365 pKa = 7.23 LSKK368 pKa = 10.56 EE369 pKa = 4.22 NQKK372 pKa = 10.33 RR373 pKa = 11.84 AVSFFEE379 pKa = 4.02 TALEE383 pKa = 4.41 KK384 pKa = 10.6 IKK386 pKa = 11.08 SSS388 pKa = 3.4
Molecular weight: 44.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.882
IPC2_protein 9.063
IPC_protein 8.946
Toseland 9.677
ProMoST 9.414
Dawson 9.926
Bjellqvist 9.604
Wikipedia 10.101
Rodwell 10.321
Grimsley 10.014
Solomon 9.94
Lehninger 9.897
Nozaki 9.692
DTASelect 9.589
Thurlkill 9.765
EMBOSS 10.101
Sillero 9.838
Patrickios 5.296
IPC_peptide 9.94
IPC2_peptide 8.112
IPC2.peptide.svr19 8.114
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
3352
53
414
186.2
21.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.117 ± 0.407
0.537 ± 0.128
6.175 ± 0.453
8.592 ± 0.564
5.221 ± 0.569
4.266 ± 0.523
1.7 ± 0.256
7.339 ± 0.601
10.322 ± 0.474
10.024 ± 0.324
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.058 ± 0.264
5.967 ± 0.329
1.969 ± 0.286
4.475 ± 0.291
4.415 ± 0.29
6.623 ± 0.37
6.205 ± 0.526
4.893 ± 0.41
0.626 ± 0.126
4.475 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here