Staphylococcus phage SA12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; unclassified Dubowvirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4V7J5|S4V7J5_9CAUD RecT family recombinase OS=Staphylococcus phage SA12 OX=1347760 GN=SA12_007 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 3.17TYY5 pKa = 11.19KK6 pKa = 10.88SYY8 pKa = 11.44LLAVLCFTVLAIVLMPFLYY27 pKa = 8.54FTTAWSIAGFASIGTFIFYY46 pKa = 10.44KK47 pKa = 10.13EE48 pKa = 3.93YY49 pKa = 10.48FYY51 pKa = 11.51GVDD54 pKa = 3.63DD55 pKa = 4.14

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4V978|S4V978_9CAUD dUTP diphosphatase OS=Staphylococcus phage SA12 OX=1347760 GN=SA12_018 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 8.57TARR6 pKa = 11.84IVRR9 pKa = 11.84IHH11 pKa = 7.01DD12 pKa = 3.16KK13 pKa = 10.25PYY15 pKa = 9.91RR16 pKa = 11.84FSKK19 pKa = 11.02FEE21 pKa = 3.76MEE23 pKa = 5.0LIEE26 pKa = 4.34SHH28 pKa = 6.88GITAGMVSKK37 pKa = 10.43RR38 pKa = 11.84VKK40 pKa = 10.67DD41 pKa = 3.3GWEE44 pKa = 3.56LHH46 pKa = 5.97EE47 pKa = 6.15AMDD50 pKa = 4.24APEE53 pKa = 4.22GTRR56 pKa = 11.84LSEE59 pKa = 3.86YY60 pKa = 10.23RR61 pKa = 11.84EE62 pKa = 3.95KK63 pKa = 10.39KK64 pKa = 8.51TIEE67 pKa = 3.99RR68 pKa = 11.84LEE70 pKa = 3.92QARR73 pKa = 11.84LEE75 pKa = 4.26RR76 pKa = 11.84KK77 pKa = 9.75LEE79 pKa = 4.03RR80 pKa = 11.84KK81 pKa = 9.41RR82 pKa = 11.84KK83 pKa = 9.67RR84 pKa = 11.84EE85 pKa = 3.71AEE87 pKa = 3.97LRR89 pKa = 11.84RR90 pKa = 11.84KK91 pKa = 9.76KK92 pKa = 10.2PHH94 pKa = 6.56LFNVPQKK101 pKa = 10.45HH102 pKa = 4.8PRR104 pKa = 11.84GRR106 pKa = 11.84YY107 pKa = 8.74ACWFDD112 pKa = 3.2TTYY115 pKa = 11.61NQMFKK120 pKa = 10.07KK121 pKa = 9.13WQEE124 pKa = 3.59AA125 pKa = 3.35

Molecular weight: 15.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13211

48

1154

227.8

26.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.775 ± 0.473

0.507 ± 0.08

6.245 ± 0.364

7.804 ± 0.575

4.156 ± 0.173

5.359 ± 0.287

1.605 ± 0.146

7.312 ± 0.348

9.235 ± 0.356

7.607 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.153

7.093 ± 0.295

2.763 ± 0.238

4.103 ± 0.239

4.11 ± 0.239

5.753 ± 0.214

6.472 ± 0.244

6.336 ± 0.299

1.166 ± 0.196

4.186 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski