Amino acid dipepetide frequency for Pectobacterium bacteriophage PM2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.252AlaAla: 4.252 ± 0.327
0.336AlaCys: 0.336 ± 0.08
3.32AlaAsp: 3.32 ± 0.208
4.178AlaGlu: 4.178 ± 0.322
2.518AlaPhe: 2.518 ± 0.207
4.029AlaGly: 4.029 ± 0.365
1.082AlaHis: 1.082 ± 0.148
4.439AlaIle: 4.439 ± 0.356
4.961AlaLys: 4.961 ± 0.33
5.316AlaLeu: 5.316 ± 0.4
1.455AlaMet: 1.455 ± 0.146
3.003AlaAsn: 3.003 ± 0.251
2.462AlaPro: 2.462 ± 0.225
2.089AlaGln: 2.089 ± 0.218
2.704AlaArg: 2.704 ± 0.24
3.954AlaSer: 3.954 ± 0.255
3.618AlaThr: 3.618 ± 0.412
4.047AlaVal: 4.047 ± 0.329
0.69AlaTrp: 0.69 ± 0.128
2.313AlaTyr: 2.313 ± 0.185
0.0AlaXaa: 0.0 ± 0.0
Cys
0.69CysAla: 0.69 ± 0.131
0.187CysCys: 0.187 ± 0.063
0.914CysAsp: 0.914 ± 0.126
0.877CysGlu: 0.877 ± 0.126
0.727CysPhe: 0.727 ± 0.14
0.56CysGly: 0.56 ± 0.106
0.336CysHis: 0.336 ± 0.086
0.485CysIle: 0.485 ± 0.094
0.877CysLys: 0.877 ± 0.131
0.634CysLeu: 0.634 ± 0.1
0.317CysMet: 0.317 ± 0.078
0.597CysAsn: 0.597 ± 0.114
0.448CysPro: 0.448 ± 0.102
0.354CysGln: 0.354 ± 0.087
0.541CysArg: 0.541 ± 0.088
0.989CysSer: 0.989 ± 0.144
0.653CysThr: 0.653 ± 0.111
0.597CysVal: 0.597 ± 0.104
0.112CysTrp: 0.112 ± 0.041
0.336CysTyr: 0.336 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
3.469AspAla: 3.469 ± 0.294
0.653AspCys: 0.653 ± 0.108
3.973AspAsp: 3.973 ± 0.339
4.159AspGlu: 4.159 ± 0.231
3.581AspPhe: 3.581 ± 0.295
4.103AspGly: 4.103 ± 0.253
0.914AspHis: 0.914 ± 0.143
5.427AspIle: 5.427 ± 0.326
4.271AspLys: 4.271 ± 0.26
4.607AspLeu: 4.607 ± 0.324
1.66AspMet: 1.66 ± 0.192
3.283AspAsn: 3.283 ± 0.229
2.108AspPro: 2.108 ± 0.223
1.846AspGln: 1.846 ± 0.156
2.294AspArg: 2.294 ± 0.197
4.327AspSer: 4.327 ± 0.273
3.152AspThr: 3.152 ± 0.257
3.488AspVal: 3.488 ± 0.283
1.156AspTrp: 1.156 ± 0.164
2.928AspTyr: 2.928 ± 0.281
0.0AspXaa: 0.0 ± 0.0
Glu
4.439GluAla: 4.439 ± 0.299
0.895GluCys: 0.895 ± 0.17
4.047GluAsp: 4.047 ± 0.308
4.943GluGlu: 4.943 ± 0.405
3.6GluPhe: 3.6 ± 0.252
3.581GluGly: 3.581 ± 0.257
0.989GluHis: 0.989 ± 0.133
6.099GluIle: 6.099 ± 0.324
4.439GluLys: 4.439 ± 0.32
6.752GluLeu: 6.752 ± 0.36
2.126GluMet: 2.126 ± 0.176
4.197GluAsn: 4.197 ± 0.232
1.884GluPro: 1.884 ± 0.179
2.387GluGln: 2.387 ± 0.222
2.76GluArg: 2.76 ± 0.223
4.625GluSer: 4.625 ± 0.341
4.327GluThr: 4.327 ± 0.31
4.066GluVal: 4.066 ± 0.263
0.951GluTrp: 0.951 ± 0.131
3.637GluTyr: 3.637 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
2.723PheAla: 2.723 ± 0.204
0.429PheCys: 0.429 ± 0.09
2.816PheAsp: 2.816 ± 0.223
3.618PheGlu: 3.618 ± 0.269
1.735PhePhe: 1.735 ± 0.214
2.835PheGly: 2.835 ± 0.224
0.56PheHis: 0.56 ± 0.111
4.066PheIle: 4.066 ± 0.249
4.178PheLys: 4.178 ± 0.338
2.891PheLeu: 2.891 ± 0.278
1.324PheMet: 1.324 ± 0.166
3.469PheAsn: 3.469 ± 0.255
1.156PhePro: 1.156 ± 0.138
1.175PheGln: 1.175 ± 0.169
1.791PheArg: 1.791 ± 0.165
3.245PheSer: 3.245 ± 0.241
2.63PheThr: 2.63 ± 0.242
2.779PheVal: 2.779 ± 0.232
0.765PheTrp: 0.765 ± 0.107
1.902PheTyr: 1.902 ± 0.194
0.0PheXaa: 0.0 ± 0.0
Gly
2.63GlyAla: 2.63 ± 0.247
0.541GlyCys: 0.541 ± 0.103
3.749GlyAsp: 3.749 ± 0.373
3.768GlyGlu: 3.768 ± 0.288
2.798GlyPhe: 2.798 ± 0.247
2.984GlyGly: 2.984 ± 0.306
0.97GlyHis: 0.97 ± 0.141
4.327GlyIle: 4.327 ± 0.239
3.879GlyLys: 3.879 ± 0.302
4.887GlyLeu: 4.887 ± 0.321
1.473GlyMet: 1.473 ± 0.165
2.947GlyAsn: 2.947 ± 0.306
1.884GlyPro: 1.884 ± 0.185
2.014GlyGln: 2.014 ± 0.193
2.779GlyArg: 2.779 ± 0.229
4.234GlySer: 4.234 ± 0.275
3.973GlyThr: 3.973 ± 0.32
3.991GlyVal: 3.991 ± 0.294
1.063GlyTrp: 1.063 ± 0.157
3.021GlyTyr: 3.021 ± 0.206
0.0GlyXaa: 0.0 ± 0.0
His
0.802HisAla: 0.802 ± 0.14
0.28HisCys: 0.28 ± 0.071
0.877HisAsp: 0.877 ± 0.129
0.951HisGlu: 0.951 ± 0.108
0.802HisPhe: 0.802 ± 0.142
1.026HisGly: 1.026 ± 0.123
0.298HisHis: 0.298 ± 0.07
1.623HisIle: 1.623 ± 0.2
1.399HisLys: 1.399 ± 0.161
1.38HisLeu: 1.38 ± 0.151
0.336HisMet: 0.336 ± 0.077
0.783HisAsn: 0.783 ± 0.119
0.989HisPro: 0.989 ± 0.125
0.597HisGln: 0.597 ± 0.098
0.709HisArg: 0.709 ± 0.114
1.287HisSer: 1.287 ± 0.159
0.69HisThr: 0.69 ± 0.111
1.063HisVal: 1.063 ± 0.127
0.242HisTrp: 0.242 ± 0.059
0.709HisTyr: 0.709 ± 0.116
0.0HisXaa: 0.0 ± 0.0
Ile
4.756IleAla: 4.756 ± 0.353
0.895IleCys: 0.895 ± 0.136
5.633IleAsp: 5.633 ± 0.333
5.558IleGlu: 5.558 ± 0.303
3.04IlePhe: 3.04 ± 0.243
3.768IleGly: 3.768 ± 0.257
1.38IleHis: 1.38 ± 0.158
6.024IleIle: 6.024 ± 0.364
6.789IleLys: 6.789 ± 0.439
5.334IleLeu: 5.334 ± 0.284
1.846IleMet: 1.846 ± 0.169
5.148IleAsn: 5.148 ± 0.274
2.928IlePro: 2.928 ± 0.245
2.723IleGln: 2.723 ± 0.247
3.32IleArg: 3.32 ± 0.259
5.968IleSer: 5.968 ± 0.351
4.42IleThr: 4.42 ± 0.34
4.7IleVal: 4.7 ± 0.278
0.541IleTrp: 0.541 ± 0.117
2.891IleTyr: 2.891 ± 0.225
0.0IleXaa: 0.0 ± 0.0
Lys
5.372LysAla: 5.372 ± 0.383
0.933LysCys: 0.933 ± 0.128
5.017LysAsp: 5.017 ± 0.318
6.491LysGlu: 6.491 ± 0.446
3.6LysPhe: 3.6 ± 0.252
3.973LysGly: 3.973 ± 0.228
1.324LysHis: 1.324 ± 0.168
5.856LysIle: 5.856 ± 0.396
5.595LysLys: 5.595 ± 0.396
5.95LysLeu: 5.95 ± 0.338
2.555LysMet: 2.555 ± 0.197
5.148LysAsn: 5.148 ± 0.321
2.033LysPro: 2.033 ± 0.186
2.499LysGln: 2.499 ± 0.226
3.096LysArg: 3.096 ± 0.269
5.036LysSer: 5.036 ± 0.347
4.029LysThr: 4.029 ± 0.248
4.663LysVal: 4.663 ± 0.354
0.989LysTrp: 0.989 ± 0.139
3.301LysTyr: 3.301 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
4.905LeuAla: 4.905 ± 0.378
0.914LeuCys: 0.914 ± 0.142
4.756LeuAsp: 4.756 ± 0.302
5.707LeuGlu: 5.707 ± 0.347
3.506LeuPhe: 3.506 ± 0.263
4.551LeuGly: 4.551 ± 0.326
1.324LeuHis: 1.324 ± 0.135
4.737LeuIle: 4.737 ± 0.269
7.069LeuLys: 7.069 ± 0.384
5.017LeuLeu: 5.017 ± 0.33
2.35LeuMet: 2.35 ± 0.206
4.756LeuAsn: 4.756 ± 0.267
3.077LeuPro: 3.077 ± 0.219
2.704LeuGln: 2.704 ± 0.227
3.115LeuArg: 3.115 ± 0.198
5.633LeuSer: 5.633 ± 0.355
4.476LeuThr: 4.476 ± 0.322
4.887LeuVal: 4.887 ± 0.322
0.877LeuTrp: 0.877 ± 0.12
3.04LeuTyr: 3.04 ± 0.26
0.0LeuXaa: 0.0 ± 0.0
Met
2.164MetAla: 2.164 ± 0.194
0.336MetCys: 0.336 ± 0.076
1.567MetAsp: 1.567 ± 0.174
1.455MetGlu: 1.455 ± 0.179
1.343MetPhe: 1.343 ± 0.155
1.38MetGly: 1.38 ± 0.165
0.392MetHis: 0.392 ± 0.081
1.94MetIle: 1.94 ± 0.181
2.443MetLys: 2.443 ± 0.219
2.108MetLeu: 2.108 ± 0.212
0.615MetMet: 0.615 ± 0.115
1.772MetAsn: 1.772 ± 0.186
0.802MetPro: 0.802 ± 0.108
1.044MetGln: 1.044 ± 0.134
0.97MetArg: 0.97 ± 0.133
2.052MetSer: 2.052 ± 0.221
1.511MetThr: 1.511 ± 0.174
1.417MetVal: 1.417 ± 0.151
0.298MetTrp: 0.298 ± 0.068
1.026MetTyr: 1.026 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
3.301AsnAla: 3.301 ± 0.28
0.56AsnCys: 0.56 ± 0.12
3.264AsnAsp: 3.264 ± 0.291
4.588AsnGlu: 4.588 ± 0.278
2.816AsnPhe: 2.816 ± 0.228
3.786AsnGly: 3.786 ± 0.281
0.951AsnHis: 0.951 ± 0.124
4.943AsnIle: 4.943 ± 0.326
4.085AsnLys: 4.085 ± 0.295
4.681AsnLeu: 4.681 ± 0.344
1.66AsnMet: 1.66 ± 0.192
3.488AsnAsn: 3.488 ± 0.281
2.35AsnPro: 2.35 ± 0.259
2.052AsnGln: 2.052 ± 0.216
2.481AsnArg: 2.481 ± 0.188
4.215AsnSer: 4.215 ± 0.263
3.32AsnThr: 3.32 ± 0.278
3.712AsnVal: 3.712 ± 0.256
0.727AsnTrp: 0.727 ± 0.135
2.555AsnTyr: 2.555 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
1.865ProAla: 1.865 ± 0.22
0.373ProCys: 0.373 ± 0.097
2.219ProAsp: 2.219 ± 0.168
3.245ProGlu: 3.245 ± 0.227
1.492ProPhe: 1.492 ± 0.18
2.201ProGly: 2.201 ± 0.209
0.709ProHis: 0.709 ± 0.109
2.537ProIle: 2.537 ± 0.238
2.518ProLys: 2.518 ± 0.233
2.145ProLeu: 2.145 ± 0.219
0.783ProMet: 0.783 ± 0.123
2.052ProAsn: 2.052 ± 0.223
0.989ProPro: 0.989 ± 0.147
0.821ProGln: 0.821 ± 0.132
1.25ProArg: 1.25 ± 0.147
2.406ProSer: 2.406 ± 0.169
2.219ProThr: 2.219 ± 0.2
2.219ProVal: 2.219 ± 0.205
0.634ProTrp: 0.634 ± 0.129
1.585ProTyr: 1.585 ± 0.191
0.0ProXaa: 0.0 ± 0.0
Gln
2.406GlnAla: 2.406 ± 0.257
0.298GlnCys: 0.298 ± 0.076
1.809GlnAsp: 1.809 ± 0.203
2.387GlnGlu: 2.387 ± 0.191
1.884GlnPhe: 1.884 ± 0.158
1.753GlnGly: 1.753 ± 0.157
0.541GlnHis: 0.541 ± 0.112
2.798GlnIle: 2.798 ± 0.232
2.425GlnLys: 2.425 ± 0.217
2.872GlnLeu: 2.872 ± 0.251
0.951GlnMet: 0.951 ± 0.152
1.417GlnAsn: 1.417 ± 0.174
1.007GlnPro: 1.007 ± 0.133
0.933GlnGln: 0.933 ± 0.137
1.548GlnArg: 1.548 ± 0.147
1.977GlnSer: 1.977 ± 0.174
1.902GlnThr: 1.902 ± 0.176
2.182GlnVal: 2.182 ± 0.219
0.709GlnTrp: 0.709 ± 0.112
1.66GlnTyr: 1.66 ± 0.149
0.0GlnXaa: 0.0 ± 0.0
Arg
2.537ArgAla: 2.537 ± 0.257
0.448ArgCys: 0.448 ± 0.094
2.555ArgAsp: 2.555 ± 0.242
2.704ArgGlu: 2.704 ± 0.246
1.567ArgPhe: 1.567 ± 0.16
2.164ArgGly: 2.164 ± 0.225
0.802ArgHis: 0.802 ± 0.134
3.395ArgIle: 3.395 ± 0.257
3.059ArgLys: 3.059 ± 0.278
3.395ArgLeu: 3.395 ± 0.229
1.026ArgMet: 1.026 ± 0.139
2.425ArgAsn: 2.425 ± 0.193
1.455ArgPro: 1.455 ± 0.154
1.529ArgGln: 1.529 ± 0.142
1.996ArgArg: 1.996 ± 0.218
2.947ArgSer: 2.947 ± 0.193
2.313ArgThr: 2.313 ± 0.193
2.686ArgVal: 2.686 ± 0.23
0.634ArgTrp: 0.634 ± 0.107
1.716ArgTyr: 1.716 ± 0.147
0.0ArgXaa: 0.0 ± 0.0
Ser
3.432SerAla: 3.432 ± 0.226
0.839SerCys: 0.839 ± 0.153
4.103SerAsp: 4.103 ± 0.273
4.551SerGlu: 4.551 ± 0.328
3.152SerPhe: 3.152 ± 0.265
4.775SerGly: 4.775 ± 0.308
1.1SerHis: 1.1 ± 0.132
5.614SerIle: 5.614 ± 0.351
5.801SerLys: 5.801 ± 0.408
6.118SerLeu: 6.118 ± 0.302
1.921SerMet: 1.921 ± 0.166
4.047SerAsn: 4.047 ± 0.309
2.07SerPro: 2.07 ± 0.206
2.108SerGln: 2.108 ± 0.165
2.779SerArg: 2.779 ± 0.225
5.483SerSer: 5.483 ± 0.442
4.234SerThr: 4.234 ± 0.33
4.271SerVal: 4.271 ± 0.317
0.951SerTrp: 0.951 ± 0.139
3.077SerTyr: 3.077 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
3.488ThrAla: 3.488 ± 0.279
0.597ThrCys: 0.597 ± 0.115
3.227ThrAsp: 3.227 ± 0.272
3.656ThrGlu: 3.656 ± 0.213
2.518ThrPhe: 2.518 ± 0.254
3.861ThrGly: 3.861 ± 0.289
0.97ThrHis: 0.97 ± 0.14
4.402ThrIle: 4.402 ± 0.305
4.42ThrLys: 4.42 ± 0.294
4.775ThrLeu: 4.775 ± 0.368
1.082ThrMet: 1.082 ± 0.138
3.581ThrAsn: 3.581 ± 0.255
2.164ThrPro: 2.164 ± 0.257
2.201ThrGln: 2.201 ± 0.269
2.518ThrArg: 2.518 ± 0.213
4.066ThrSer: 4.066 ± 0.303
3.077ThrThr: 3.077 ± 0.255
3.786ThrVal: 3.786 ± 0.26
0.877ThrTrp: 0.877 ± 0.138
2.089ThrTyr: 2.089 ± 0.179
0.0ThrXaa: 0.0 ± 0.0
Val
3.581ValAla: 3.581 ± 0.289
0.933ValCys: 0.933 ± 0.116
3.805ValAsp: 3.805 ± 0.267
4.737ValGlu: 4.737 ± 0.29
2.704ValPhe: 2.704 ± 0.273
3.264ValGly: 3.264 ± 0.25
1.082ValHis: 1.082 ± 0.131
4.961ValIle: 4.961 ± 0.28
4.849ValLys: 4.849 ± 0.314
4.383ValLeu: 4.383 ± 0.242
1.548ValMet: 1.548 ± 0.15
3.749ValAsn: 3.749 ± 0.266
2.238ValPro: 2.238 ± 0.199
2.219ValGln: 2.219 ± 0.216
2.462ValArg: 2.462 ± 0.214
4.141ValSer: 4.141 ± 0.282
3.432ValThr: 3.432 ± 0.286
4.402ValVal: 4.402 ± 0.257
0.97ValTrp: 0.97 ± 0.134
2.928ValTyr: 2.928 ± 0.233
0.0ValXaa: 0.0 ± 0.0
Trp
1.138TrpAla: 1.138 ± 0.152
0.224TrpCys: 0.224 ± 0.058
0.951TrpAsp: 0.951 ± 0.12
0.858TrpGlu: 0.858 ± 0.123
0.671TrpPhe: 0.671 ± 0.101
0.578TrpGly: 0.578 ± 0.139
0.261TrpHis: 0.261 ± 0.06
0.895TrpIle: 0.895 ± 0.117
1.082TrpLys: 1.082 ± 0.144
0.895TrpLeu: 0.895 ± 0.144
0.485TrpMet: 0.485 ± 0.098
0.839TrpAsn: 0.839 ± 0.123
0.485TrpPro: 0.485 ± 0.094
0.578TrpGln: 0.578 ± 0.103
0.653TrpArg: 0.653 ± 0.099
0.858TrpSer: 0.858 ± 0.149
0.765TrpThr: 0.765 ± 0.133
0.933TrpVal: 0.933 ± 0.138
0.242TrpTrp: 0.242 ± 0.061
0.597TrpTyr: 0.597 ± 0.098
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.816TyrAla: 2.816 ± 0.249
0.56TyrCys: 0.56 ± 0.094
2.798TyrAsp: 2.798 ± 0.251
2.313TyrGlu: 2.313 ± 0.253
2.052TyrPhe: 2.052 ± 0.214
2.742TyrGly: 2.742 ± 0.215
0.858TyrHis: 0.858 ± 0.118
3.096TyrIle: 3.096 ± 0.254
3.32TyrLys: 3.32 ± 0.227
3.264TyrLeu: 3.264 ± 0.243
1.1TyrMet: 1.1 ± 0.149
2.835TyrAsn: 2.835 ± 0.208
1.735TyrPro: 1.735 ± 0.177
1.585TyrGln: 1.585 ± 0.18
1.585TyrArg: 1.585 ± 0.18
2.984TyrSer: 2.984 ± 0.227
2.611TyrThr: 2.611 ± 0.247
2.499TyrVal: 2.499 ± 0.2
0.56TyrTrp: 0.56 ± 0.09
1.791TyrTyr: 1.791 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 291 proteins (53617 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski