Mucilaginibacter pedocola

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S9PAT1|A0A1S9PAT1_9SPHI Uncharacterized protein OS=Mucilaginibacter pedocola OX=1792845 GN=BC343_10515 PE=4 SV=1
MM1 pKa = 7.4PQFSINIPCPFDD13 pKa = 3.01KK14 pKa = 10.87AYY16 pKa = 10.51ALKK19 pKa = 10.38IEE21 pKa = 4.61DD22 pKa = 4.55DD23 pKa = 3.73GRR25 pKa = 11.84VAYY28 pKa = 9.88AYY30 pKa = 8.56LTFYY34 pKa = 11.16DD35 pKa = 4.87DD36 pKa = 3.54VVSDD40 pKa = 2.99VWLYY44 pKa = 11.38NRR46 pKa = 11.84LPAPTVSFLNTDD58 pKa = 4.21DD59 pKa = 5.35GPALNPAEE67 pKa = 4.09YY68 pKa = 9.94LKK70 pKa = 10.84SDD72 pKa = 3.64TAVTPMSTEE81 pKa = 3.79ADD83 pKa = 3.49ITCEE87 pKa = 3.61WDD89 pKa = 3.0EE90 pKa = 4.42SKK92 pKa = 11.2EE93 pKa = 3.9GDD95 pKa = 3.44IEE97 pKa = 4.57VAVLLHH103 pKa = 6.21GKK105 pKa = 9.7FIAEE109 pKa = 4.7LLPGAMPGSSVLVAKK124 pKa = 10.31DD125 pKa = 3.53GPLALVYY132 pKa = 10.74

Molecular weight: 14.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S9PM62|A0A1S9PM62_9SPHI Amino acid permease OS=Mucilaginibacter pedocola OX=1792845 GN=BC343_03045 PE=4 SV=1
MM1 pKa = 7.03KK2 pKa = 9.98TIILSLLIAFSAVVAQAQTRR22 pKa = 11.84VVVRR26 pKa = 11.84HH27 pKa = 5.7RR28 pKa = 11.84PHH30 pKa = 5.71RR31 pKa = 11.84TVAVVRR37 pKa = 11.84PAVIAPVVVARR48 pKa = 11.84PIAVVRR54 pKa = 11.84PVVVARR60 pKa = 11.84PVVVARR66 pKa = 11.84PVRR69 pKa = 11.84RR70 pKa = 11.84VVVVHH75 pKa = 6.69

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5893

0

5893

2023964

48

6132

343.5

38.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.458 ± 0.049

0.768 ± 0.011

5.341 ± 0.021

5.359 ± 0.043

4.784 ± 0.029

7.094 ± 0.042

1.845 ± 0.016

6.723 ± 0.027

6.728 ± 0.038

9.386 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.017

5.482 ± 0.028

4.001 ± 0.02

3.726 ± 0.02

3.997 ± 0.021

6.02 ± 0.03

6.058 ± 0.054

6.531 ± 0.029

1.196 ± 0.013

4.215 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski