Mulberry mosaic dwarf associated virus
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5BTW9|A0A0D5BTW9_9GEMI V2 protein OS=Mulberry mosaic dwarf associated virus OX=1631303 PE=4 SV=1
MM1 pKa = 7.59 ASSSNFRR8 pKa = 11.84 LAAKK12 pKa = 10.11 NVFLTFPQCPEE23 pKa = 3.95 SITWVMSHH31 pKa = 7.42 LLQILNSYY39 pKa = 6.28 TVKK42 pKa = 9.67 YY43 pKa = 10.74 ACVAEE48 pKa = 4.75 EE49 pKa = 3.91 KK50 pKa = 10.78 HH51 pKa = 6.71 KK52 pKa = 11.14 DD53 pKa = 3.82 DD54 pKa = 4.22 NSHH57 pKa = 5.57 FHH59 pKa = 7.5 AIVQLDD65 pKa = 3.72 KK66 pKa = 11.18 KK67 pKa = 11.2 LEE69 pKa = 4.05 TRR71 pKa = 11.84 NPNFFDD77 pKa = 4.15 LPQEE81 pKa = 4.42 HH82 pKa = 7.05 SSGTYY87 pKa = 9.12 HH88 pKa = 7.52 PNVQKK93 pKa = 10.63 CSSPAAVRR101 pKa = 11.84 KK102 pKa = 9.1 YY103 pKa = 9.62 IQKK106 pKa = 8.64 EE107 pKa = 3.98 GHH109 pKa = 6.37 FIEE112 pKa = 6.01 HH113 pKa = 6.84 GEE115 pKa = 3.96 FNTRR119 pKa = 11.84 GRR121 pKa = 11.84 SPVASAEE128 pKa = 4.26 KK129 pKa = 10.25 IFGEE133 pKa = 4.16 ILTLATDD140 pKa = 3.81 EE141 pKa = 4.59 EE142 pKa = 5.12 SFLALVRR149 pKa = 11.84 EE150 pKa = 4.55 RR151 pKa = 11.84 RR152 pKa = 11.84 PQDD155 pKa = 3.35 YY156 pKa = 10.58 VLRR159 pKa = 11.84 WPSITGFARR168 pKa = 11.84 DD169 pKa = 3.33 HH170 pKa = 5.48 YY171 pKa = 10.1 RR172 pKa = 11.84 RR173 pKa = 11.84 RR174 pKa = 11.84 SIPYY178 pKa = 7.95 VPRR181 pKa = 11.84 WTDD184 pKa = 3.76 FPGLPEE190 pKa = 6.34 PIQQWAKK197 pKa = 11.29 DD198 pKa = 3.78 NILFEE203 pKa = 4.94 PEE205 pKa = 4.33 SKK207 pKa = 9.99 PDD209 pKa = 3.61 RR210 pKa = 11.84 PISLYY215 pKa = 9.8 ICGPTRR221 pKa = 11.84 SGKK224 pKa = 6.56 TQWARR229 pKa = 11.84 SLGRR233 pKa = 11.84 HH234 pKa = 5.29 NYY236 pKa = 7.43 FTGSLSFLDD245 pKa = 3.88 YY246 pKa = 11.47 DD247 pKa = 5.47 DD248 pKa = 4.8 FALYY252 pKa = 10.64 NVIDD256 pKa = 4.96 DD257 pKa = 4.16 IEE259 pKa = 4.33 YY260 pKa = 10.95 EE261 pKa = 4.34 KK262 pKa = 10.76 ISTSWFKK269 pKa = 11.37 SLLGAQKK276 pKa = 10.67 DD277 pKa = 3.7 IILKK281 pKa = 9.29 GKK283 pKa = 8.39 YY284 pKa = 8.83 MKK286 pKa = 10.46 DD287 pKa = 3.32 FRR289 pKa = 11.84 ISGGIPCIVLVNEE302 pKa = 4.06 DD303 pKa = 3.69 MDD305 pKa = 3.95 WVVRR309 pKa = 11.84 MASTLKK315 pKa = 10.26 PWFDD319 pKa = 4.71 DD320 pKa = 3.66 NVLVYY325 pKa = 10.71 YY326 pKa = 9.85 MSASDD331 pKa = 4.0 KK332 pKa = 10.91 FYY334 pKa = 11.37
Molecular weight: 38.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.559
IPC2_protein 6.59
IPC_protein 6.766
Toseland 6.839
ProMoST 7.307
Dawson 7.263
Bjellqvist 7.146
Wikipedia 7.234
Rodwell 7.249
Grimsley 6.956
Solomon 7.307
Lehninger 7.307
Nozaki 7.468
DTASelect 7.541
Thurlkill 7.571
EMBOSS 7.614
Sillero 7.658
Patrickios 4.202
IPC_peptide 7.307
IPC2_peptide 7.088
IPC2.peptide.svr19 7.003
Protein with the highest isoelectric point:
>tr|A0A0D5BV09|A0A0D5BV09_9GEMI Capsid protein OS=Mulberry mosaic dwarf associated virus OX=1631303 PE=3 SV=1
MM1 pKa = 7.46 VITRR5 pKa = 11.84 SSALRR10 pKa = 11.84 SRR12 pKa = 11.84 RR13 pKa = 11.84 GWNPGTIPRR22 pKa = 11.84 RR23 pKa = 11.84 PRR25 pKa = 11.84 TVRR28 pKa = 11.84 VTRR31 pKa = 11.84 VASAMRR37 pKa = 11.84 TPWRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 GPRR46 pKa = 11.84 SSLARR51 pKa = 11.84 RR52 pKa = 11.84 KK53 pKa = 9.49 RR54 pKa = 11.84 RR55 pKa = 11.84 PINALGPVKK64 pKa = 10.48 RR65 pKa = 11.84 GTFGIKK71 pKa = 9.51 LASINHH77 pKa = 5.99 TGGNGYY83 pKa = 9.56 HH84 pKa = 6.14 LTHH87 pKa = 7.21 FDD89 pKa = 4.44 HH90 pKa = 7.7 GDD92 pKa = 3.38 DD93 pKa = 3.61 TNQRR97 pKa = 11.84 TGRR100 pKa = 11.84 KK101 pKa = 8.85 IKK103 pKa = 10.6 INGINIRR110 pKa = 11.84 GKK112 pKa = 10.76 LYY114 pKa = 10.84 LNNPRR119 pKa = 11.84 TNSYY123 pKa = 10.5 HH124 pKa = 7.26 IVRR127 pKa = 11.84 LWIIRR132 pKa = 11.84 DD133 pKa = 3.63 TRR135 pKa = 11.84 PGSEE139 pKa = 3.84 PVSFSSFMDD148 pKa = 3.23 MHH150 pKa = 7.47 DD151 pKa = 4.42 NEE153 pKa = 5.02 PMTAMVKK160 pKa = 10.18 KK161 pKa = 10.37 DD162 pKa = 2.82 WGEE165 pKa = 3.75 RR166 pKa = 11.84 FQVLKK171 pKa = 10.82 DD172 pKa = 3.56 LTFHH176 pKa = 7.1 LVGANGLSFNEE187 pKa = 4.44 DD188 pKa = 3.04 VVEE191 pKa = 4.55 EE192 pKa = 4.12 YY193 pKa = 10.88 FKK195 pKa = 11.28 FKK197 pKa = 10.84 GYY199 pKa = 10.67 VLYY202 pKa = 10.79 NHH204 pKa = 7.07 EE205 pKa = 5.1 DD206 pKa = 3.52 SGSLANVLEE215 pKa = 4.23 NGIFLYY221 pKa = 10.7 AATSHH226 pKa = 6.48 PSEE229 pKa = 4.32 NVTLTANCRR238 pKa = 11.84 VYY240 pKa = 10.61 FYY242 pKa = 10.91 DD243 pKa = 5.07 AEE245 pKa = 4.19
Molecular weight: 28.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.78
IPC_protein 10.657
Toseland 10.628
ProMoST 10.394
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.921
Grimsley 10.818
Solomon 10.877
Lehninger 10.833
Nozaki 10.613
DTASelect 10.496
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.687
Patrickios 10.599
IPC_peptide 10.877
IPC2_peptide 9.428
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1097
105
334
219.4
25.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.108 ± 0.329
2.005 ± 0.599
5.287 ± 0.464
5.834 ± 0.541
4.74 ± 0.516
5.287 ± 0.724
3.373 ± 0.345
5.378 ± 0.494
5.287 ± 0.541
7.475 ± 0.225
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.097 ± 0.311
4.74 ± 0.652
6.016 ± 0.655
3.464 ± 0.819
7.84 ± 0.869
8.204 ± 0.358
5.014 ± 0.533
5.925 ± 0.206
2.005 ± 0.314
3.92 ± 0.621
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here