Bermuda grass latent virus
Average proteome isoelectric point is 8.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L6C7T1|A0A1L6C7T1_9TOMB Capsid protein OS=Bermuda grass latent virus OX=1930269 PE=3 SV=1
MM1 pKa = 7.45 ATGKK5 pKa = 8.66 CHH7 pKa = 6.99 CPDD10 pKa = 3.96 SLWTPGLLAICIVLALCLVARR31 pKa = 11.84 STSEE35 pKa = 3.8 PPIISPPVFHH45 pKa = 6.48 TVYY48 pKa = 10.5 HH49 pKa = 5.88 CEE51 pKa = 3.8 KK52 pKa = 8.61 YY53 pKa = 10.76 QNIEE57 pKa = 3.89 VQKK60 pKa = 11.04
Molecular weight: 6.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.442
IPC2_protein 6.262
IPC_protein 6.478
Toseland 6.427
ProMoST 7.059
Dawson 6.985
Bjellqvist 6.795
Wikipedia 6.956
Rodwell 6.971
Grimsley 6.491
Solomon 7.029
Lehninger 7.044
Nozaki 7.176
DTASelect 7.263
Thurlkill 7.307
EMBOSS 7.337
Sillero 7.41
Patrickios 0.604
IPC_peptide 7.044
IPC2_peptide 7.176
IPC2.peptide.svr19 7.093
Protein with the highest isoelectric point:
>tr|A0A1L6C7S8|A0A1L6C7S8_9TOMB Putative movement protein 1 OS=Bermuda grass latent virus OX=1930269 PE=4 SV=1
MM1 pKa = 7.36 APVVVVNNLPPPGTVAARR19 pKa = 11.84 EE20 pKa = 4.15 IKK22 pKa = 10.46 VLGARR27 pKa = 11.84 VVEE30 pKa = 3.74 VDD32 pKa = 4.28 LWNEE36 pKa = 3.93 QCQLPPRR43 pKa = 11.84 TLWVRR48 pKa = 11.84 RR49 pKa = 11.84 DD50 pKa = 3.36 PRR52 pKa = 11.84 AWGVDD57 pKa = 2.74 RR58 pKa = 11.84 DD59 pKa = 4.13 GQVWLTRR66 pKa = 11.84 KK67 pKa = 10.11 SYY69 pKa = 10.44 FKK71 pKa = 10.87 SLLVPAVTPYY81 pKa = 10.76 SPSPSFQTCSIRR93 pKa = 11.84 QTVRR97 pKa = 11.84 LIPDD101 pKa = 3.25 VRR103 pKa = 11.84 NSSQGTPHH111 pKa = 6.56 CTPNTNGTCWHH122 pKa = 6.63 SNGHH126 pKa = 4.86 QVVQRR131 pKa = 11.84 LHH133 pKa = 6.99 LEE135 pKa = 3.8 TWFF138 pKa = 3.91
Molecular weight: 15.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 8.858
IPC_protein 9.092
Toseland 9.472
ProMoST 9.37
Dawson 9.765
Bjellqvist 9.692
Wikipedia 9.94
Rodwell 9.867
Grimsley 9.78
Solomon 9.911
Lehninger 9.882
Nozaki 9.897
DTASelect 9.545
Thurlkill 9.663
EMBOSS 9.926
Sillero 9.838
Patrickios 7.6
IPC_peptide 9.897
IPC2_peptide 9.136
IPC2.peptide.svr19 7.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1755
60
912
292.5
33.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.578 ± 0.734
2.279 ± 0.386
4.387 ± 0.395
5.356 ± 1.052
3.818 ± 0.469
5.47 ± 0.541
2.564 ± 0.228
4.558 ± 0.533
6.04 ± 0.935
8.547 ± 0.834
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.652 ± 0.261
3.248 ± 0.556
5.527 ± 1.218
4.843 ± 0.616
7.293 ± 0.409
7.35 ± 0.657
7.009 ± 0.71
6.895 ± 1.022
1.481 ± 0.384
4.046 ± 0.578
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here