Streptomyces sp. GP55

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N3UI34|A0A2N3UI34_9ACTN Putative ATP-grasp superfamily ATP-dependent carboligase OS=Streptomyces sp. GP55 OX=1798190 GN=AXZ45_1652 PE=4 SV=1
MM1 pKa = 7.39SVTSGADD8 pKa = 2.88AAEE11 pKa = 4.27PLEE14 pKa = 4.07VWIDD18 pKa = 3.44QDD20 pKa = 3.99LCTGDD25 pKa = 4.65GICVQYY31 pKa = 10.96APEE34 pKa = 4.18VFEE37 pKa = 5.9LDD39 pKa = 3.05IDD41 pKa = 3.65GLAYY45 pKa = 10.69VKK47 pKa = 11.17GEE49 pKa = 4.07DD50 pKa = 4.75DD51 pKa = 4.81EE52 pKa = 6.03LRR54 pKa = 11.84QQPGEE59 pKa = 4.1TAPVPLTLLQDD70 pKa = 3.53IVDD73 pKa = 4.32SARR76 pKa = 11.84EE77 pKa = 3.97CPGDD81 pKa = 4.2CIHH84 pKa = 6.36VRR86 pKa = 11.84RR87 pKa = 11.84VSDD90 pKa = 3.3RR91 pKa = 11.84VEE93 pKa = 4.23VYY95 pKa = 10.89GPDD98 pKa = 3.33ADD100 pKa = 3.69

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N3ULY8|A0A2N3ULY8_9ACTN Uncharacterized protein (DUF1330 family) OS=Streptomyces sp. GP55 OX=1798190 GN=AXZ45_3166 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7642

0

7642

2550680

29

8002

333.8

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.205 ± 0.047

0.812 ± 0.008

5.642 ± 0.023

5.406 ± 0.031

2.668 ± 0.016

9.354 ± 0.026

2.319 ± 0.015

3.123 ± 0.02

1.776 ± 0.019

10.988 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.624 ± 0.012

1.822 ± 0.015

6.376 ± 0.03

3.31 ± 0.02

7.756 ± 0.034

5.085 ± 0.022

6.053 ± 0.026

8.151 ± 0.028

1.54 ± 0.011

1.991 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski