Vulcanibacillus modesticaldus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Vulcanibacillus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D2YU79|A0A1D2YU79_9BACI Lipid kinase OS=Vulcanibacillus modesticaldus OX=337097 GN=BHF71_09440 PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.33KK3 pKa = 10.43LLIGVLVLGLAFGGAAYY20 pKa = 8.42VTYY23 pKa = 10.99ARR25 pKa = 11.84GDD27 pKa = 3.75DD28 pKa = 3.8NDD30 pKa = 3.71NLNNQFWMQPQNFDD44 pKa = 3.49DD45 pKa = 5.75AYY47 pKa = 9.16MGQYY51 pKa = 9.39MYY53 pKa = 11.02GIDD56 pKa = 4.15EE57 pKa = 4.19EE58 pKa = 4.59QMYY61 pKa = 11.02QLMEE65 pKa = 3.95QQGVDD70 pKa = 3.7VEE72 pKa = 4.34QMDD75 pKa = 3.98RR76 pKa = 11.84FMEE79 pKa = 4.26EE80 pKa = 2.98QGLDD84 pKa = 3.36VDD86 pKa = 5.33EE87 pKa = 4.82MFEE90 pKa = 4.12MMNSGADD97 pKa = 3.84FNEE100 pKa = 3.83MWQYY104 pKa = 8.87MQEE107 pKa = 4.14SNIDD111 pKa = 3.66FNEE114 pKa = 3.9MGRR117 pKa = 11.84FMEE120 pKa = 4.95DD121 pKa = 2.93QNIDD125 pKa = 3.51FGQMQIFMQQMHH137 pKa = 5.8GTRR140 pKa = 3.77

Molecular weight: 16.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D2YTI1|A0A1D2YTI1_9BACI Methylated-DNA--protein-cysteine methyltransferase OS=Vulcanibacillus modesticaldus OX=337097 GN=BHF71_09865 PE=3 SV=1
MM1 pKa = 7.25NRR3 pKa = 11.84LFQSIIAGGVVVTFAGFMLRR23 pKa = 11.84RR24 pKa = 11.84ISSAKK29 pKa = 9.63RR30 pKa = 11.84MNRR33 pKa = 11.84INKK36 pKa = 7.08MINMALHH43 pKa = 5.03MLGRR47 pKa = 11.84LGFLRR52 pKa = 11.84MMGRR56 pKa = 11.84RR57 pKa = 11.84NFVRR61 pKa = 11.84NILRR65 pKa = 11.84FRR67 pKa = 3.97

Molecular weight: 7.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2107

0

2107

627377

27

1682

297.8

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.974 ± 0.054

0.49 ± 0.017

5.328 ± 0.045

7.444 ± 0.056

4.311 ± 0.045

6.552 ± 0.048

1.71 ± 0.023

9.745 ± 0.055

8.05 ± 0.049

9.83 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.444 ± 0.021

5.167 ± 0.046

3.409 ± 0.034

3.392 ± 0.032

4.049 ± 0.039

5.705 ± 0.04

4.98 ± 0.039

6.77 ± 0.044

0.872 ± 0.019

3.779 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski