Ensete ventricosum (Abyssinian banana) (Musa ensete)
Average proteome isoelectric point is 7.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 58382 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A444F8W1|A0A444F8W1_ENSVE Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=GW17_00016912 PE=4 SV=1
MM1 pKa = 7.77 ASLLLSPPSLADD13 pKa = 3.73 PNATTMPTPSGAIQRR28 pKa = 11.84 EE29 pKa = 3.85 MDD31 pKa = 3.5 QQILATVLPDD41 pKa = 3.53 SSSNTQPFLTSPTGKK56 pKa = 8.5 YY57 pKa = 8.13 TGYY60 pKa = 11.03 LLRR63 pKa = 11.84 HH64 pKa = 6.4 KK65 pKa = 8.72 STPDD69 pKa = 2.9 SGGYY73 pKa = 10.54 GSDD76 pKa = 3.02 FCYY79 pKa = 10.39 IQIQDD84 pKa = 3.96 TVSGDD89 pKa = 4.05 SVWEE93 pKa = 4.32 SEE95 pKa = 4.39 CEE97 pKa = 4.06 PVSSANACTLVFSDD111 pKa = 4.88 AGLAVLDD118 pKa = 4.92 GSQSVWDD125 pKa = 4.0 TGASNGNNFPATLEE139 pKa = 4.03 LADD142 pKa = 4.86 LGDD145 pKa = 3.57 MTVADD150 pKa = 4.49 KK151 pKa = 11.25 DD152 pKa = 4.08 GEE154 pKa = 4.63 LVWKK158 pKa = 10.38 ASNDD162 pKa = 3.04 PRR164 pKa = 11.84 VNQGCVSPDD173 pKa = 3.45 LGSGSPTFVGGDD185 pKa = 3.58 DD186 pKa = 4.14 NSPLGQQLPPPPPYY200 pKa = 10.23 LSEE203 pKa = 4.26 GALPFAPASPPVVGDD218 pKa = 3.94 DD219 pKa = 5.71 DD220 pKa = 4.01 ITSGQAPLLAPATAPFAAPTSGDD243 pKa = 3.17 EE244 pKa = 4.25 NIPYY248 pKa = 9.12 NQQPTSYY255 pKa = 9.45 PSNGAFGQQQEE266 pKa = 4.35 QQQSAFHH273 pKa = 6.45 GLHH276 pKa = 6.19 GVNQPLLDD284 pKa = 3.87 NNAYY288 pKa = 10.48 DD289 pKa = 3.73 SGCSRR294 pKa = 11.84 KK295 pKa = 9.7 EE296 pKa = 3.7 GLVGILLAVVGHH308 pKa = 6.3 LVLRR312 pKa = 11.84 GLL314 pKa = 4.08
Molecular weight: 32.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.63
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.681
Grimsley 3.541
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 0.871
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A444DM74|A0A444DM74_ENSVE Uncharacterized protein (Fragment) OS=Ensete ventricosum OX=4639 GN=GW17_00037851 PE=4 SV=1
MM1 pKa = 7.59 GISFRR6 pKa = 11.84 VQRR9 pKa = 11.84 VPISNAALPPPFSLLGSSRR28 pKa = 11.84 GQRR31 pKa = 11.84 RR32 pKa = 11.84 FKK34 pKa = 10.85 HH35 pKa = 5.26 FVPSIVLSSLSPLLRR50 pKa = 11.84 LSLARR55 pKa = 11.84 SHH57 pKa = 6.18 IRR59 pKa = 11.84 PRR61 pKa = 11.84 HH62 pKa = 5.05 RR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 LWMASRR71 pKa = 11.84 TGII74 pKa = 4.04
Molecular weight: 8.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.618
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.34
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.251
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58382
0
58382
13105813
49
3236
224.5
24.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.07 ± 0.012
2.112 ± 0.006
4.941 ± 0.008
6.016 ± 0.013
3.733 ± 0.009
7.319 ± 0.012
2.563 ± 0.006
4.283 ± 0.009
4.885 ± 0.011
9.576 ± 0.015
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.006
3.211 ± 0.008
5.899 ± 0.014
3.306 ± 0.009
7.386 ± 0.012
9.161 ± 0.014
4.817 ± 0.007
6.633 ± 0.01
1.354 ± 0.004
2.376 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here