Stipagrostis associaed virus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345BKA4|A0A345BKA4_9VIRU Putative capsid protein OS=Stipagrostis associaed virus OX=2282646 PE=4 SV=1
MM1 pKa = 7.0YY2 pKa = 9.99RR3 pKa = 11.84IRR5 pKa = 11.84GQALLLTYY13 pKa = 10.42SQTSLDD19 pKa = 4.05QNDD22 pKa = 3.85LAAFHH27 pKa = 7.07HH28 pKa = 6.15EE29 pKa = 4.05QLPISYY35 pKa = 10.05YY36 pKa = 10.51LLANEE41 pKa = 4.18THH43 pKa = 6.25QDD45 pKa = 3.12NGRR48 pKa = 11.84HH49 pKa = 3.76YY50 pKa = 10.28HH51 pKa = 6.39VYY53 pKa = 10.94LEE55 pKa = 4.25TEE57 pKa = 3.83KK58 pKa = 11.0RR59 pKa = 11.84FDD61 pKa = 3.18ITNVRR66 pKa = 11.84RR67 pKa = 11.84FDD69 pKa = 3.95FGGEE73 pKa = 4.1HH74 pKa = 7.56PNTQVPRR81 pKa = 11.84NRR83 pKa = 11.84EE84 pKa = 3.56ATINYY89 pKa = 7.36CKK91 pKa = 10.48KK92 pKa = 10.7EE93 pKa = 3.61GDD95 pKa = 3.48YY96 pKa = 10.98WEE98 pKa = 4.92LGRR101 pKa = 11.84PATNASRR108 pKa = 11.84QQHH111 pKa = 5.6LCEE114 pKa = 4.07EE115 pKa = 4.83TTFSTGDD122 pKa = 3.17CRR124 pKa = 11.84NFEE127 pKa = 5.06KK128 pKa = 10.79EE129 pKa = 3.83IDD131 pKa = 4.04WIDD134 pKa = 3.19HH135 pKa = 6.68CITNKK140 pKa = 9.87IQFGYY145 pKa = 9.59CARR148 pKa = 11.84IWALHH153 pKa = 5.69NIKK156 pKa = 9.64PLKK159 pKa = 10.54YY160 pKa = 8.53ITNDD164 pKa = 3.43SIFNGVVTHH173 pKa = 6.16PTLMLPIQPDD183 pKa = 3.59PAKK186 pKa = 8.83ATVVIGPTGVGKK198 pKa = 7.48TVWAKK203 pKa = 10.55RR204 pKa = 11.84NSIKK208 pKa = 10.25PALLIRR214 pKa = 11.84HH215 pKa = 6.52LEE217 pKa = 4.04DD218 pKa = 4.02LKK220 pKa = 10.59TVDD223 pKa = 2.77WSYY226 pKa = 11.83LKK228 pKa = 10.07TIIFDD233 pKa = 3.93DD234 pKa = 3.46MLFRR238 pKa = 11.84HH239 pKa = 6.38WPLQTQIHH247 pKa = 6.15LLDD250 pKa = 3.62WDD252 pKa = 4.05EE253 pKa = 5.19DD254 pKa = 4.26SSIHH258 pKa = 6.37ARR260 pKa = 11.84YY261 pKa = 10.29GNITIPANTHH271 pKa = 6.93KK272 pKa = 10.57IFTCNEE278 pKa = 3.75DD279 pKa = 3.33PFIDD283 pKa = 3.55HH284 pKa = 6.48EE285 pKa = 4.88AIFRR289 pKa = 11.84RR290 pKa = 11.84IHH292 pKa = 5.84VYY294 pKa = 10.2RR295 pKa = 11.84LSVTDD300 pKa = 3.55

Molecular weight: 35.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BKA4|A0A345BKA4_9VIRU Putative capsid protein OS=Stipagrostis associaed virus OX=2282646 PE=4 SV=1
MM1 pKa = 7.97PSTITRR7 pKa = 11.84RR8 pKa = 11.84NGVSSYY14 pKa = 10.47RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84MFGPIGLRR26 pKa = 11.84AMAHH30 pKa = 5.77MGDD33 pKa = 3.72MALKK37 pKa = 9.96RR38 pKa = 11.84ASRR41 pKa = 11.84SYY43 pKa = 11.08KK44 pKa = 9.82SFGRR48 pKa = 11.84RR49 pKa = 11.84GKK51 pKa = 10.07GSSMGKK57 pKa = 9.93SNSKK61 pKa = 9.47MDD63 pKa = 3.63SAPLYY68 pKa = 8.05GTTYY72 pKa = 8.78TSKK75 pKa = 9.84VSYY78 pKa = 10.02RR79 pKa = 11.84KK80 pKa = 9.87RR81 pKa = 11.84GVSRR85 pKa = 11.84SRR87 pKa = 11.84SKK89 pKa = 10.43FGKK92 pKa = 9.82RR93 pKa = 11.84KK94 pKa = 9.21FGKK97 pKa = 9.85FLHH100 pKa = 6.33QSMKK104 pKa = 10.52LQNAQNTLAGNAFGLTTTAGSQAWSSLDD132 pKa = 3.22LCNGVEE138 pKa = 4.7LYY140 pKa = 10.7QILRR144 pKa = 11.84TQLPPSASTATMRR157 pKa = 11.84DD158 pKa = 3.0FTMALKK164 pKa = 10.65NFNLKK169 pKa = 9.65WYY171 pKa = 6.91ITNVGTTNVEE181 pKa = 3.72VEE183 pKa = 4.79IYY185 pKa = 10.23HH186 pKa = 6.06IMPRR190 pKa = 11.84RR191 pKa = 11.84DD192 pKa = 3.43VTCKK196 pKa = 10.06EE197 pKa = 3.92MSIATPSPSNGNIFANYY214 pKa = 9.57VDD216 pKa = 3.71SQYY219 pKa = 11.36PSDD222 pKa = 4.61ALGDD226 pKa = 3.91PDD228 pKa = 4.59GDD230 pKa = 3.65AVPTINSLGWTPFLNRR246 pKa = 11.84NLMRR250 pKa = 11.84QFIVKK255 pKa = 10.16KK256 pKa = 9.9VRR258 pKa = 11.84TVKK261 pKa = 9.56MAPADD266 pKa = 3.58TYY268 pKa = 9.7MEE270 pKa = 4.87RR271 pKa = 11.84DD272 pKa = 3.5SVGQKK277 pKa = 9.01MLNASRR283 pKa = 11.84LGLEE287 pKa = 4.08IFSNGATTSYY297 pKa = 11.48NDD299 pKa = 3.21VQKK302 pKa = 10.48WYY304 pKa = 11.03LKK306 pKa = 10.47GLSQIIFIKK315 pKa = 10.67LRR317 pKa = 11.84GFPNTTSGSPVSSINIAWEE336 pKa = 3.96VTTTSKK342 pKa = 10.91VIQTRR347 pKa = 11.84TGSDD351 pKa = 3.08ALIGNAA357 pKa = 4.43

Molecular weight: 39.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

657

300

357

328.5

37.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.088 ± 0.454

1.218 ± 0.471

5.327 ± 1.007

3.501 ± 1.103

4.11 ± 0.135

6.393 ± 1.239

3.044 ± 1.578

6.697 ± 1.185

5.632 ± 0.581

7.61 ± 0.435

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.892 ± 1.138

5.936 ± 0.162

4.566 ± 0.06

3.653 ± 0.209

6.849 ± 0.31

7.61 ± 2.373

8.524 ± 0.114

4.566 ± 0.341

1.826 ± 0.305

3.957 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski