Aeromonas phage 4_L372D

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Plateaulakevirus; Aeromonas virus 4L372D

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 223 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N944|A0A5B9N944_9CAUD Uncharacterized protein OS=Aeromonas phage 4_L372D OX=2588518 GN=4L372D_076 PE=4 SV=1
MM1 pKa = 7.65PVTITFHH8 pKa = 6.49KK9 pKa = 10.58VSEE12 pKa = 4.35KK13 pKa = 10.61KK14 pKa = 9.95PEE16 pKa = 4.34HH17 pKa = 5.66GQSIIWLQNTSSFGYY32 pKa = 9.93QGFNPRR38 pKa = 11.84EE39 pKa = 3.82VDD41 pKa = 4.59VEE43 pKa = 4.44YY44 pKa = 11.05QWTEE48 pKa = 3.46YY49 pKa = 10.8FLADD53 pKa = 4.47KK54 pKa = 10.71VATGNAICYY63 pKa = 9.62DD64 pKa = 3.93HH65 pKa = 7.36GDD67 pKa = 3.69EE68 pKa = 4.71TPEE71 pKa = 4.06PEE73 pKa = 3.77NGVFYY78 pKa = 10.26EE79 pKa = 4.17ISILVDD85 pKa = 3.28GWEE88 pKa = 4.85LEE90 pKa = 4.52PNDD93 pKa = 3.5LWIDD97 pKa = 3.47VEE99 pKa = 4.86EE100 pKa = 4.16YY101 pKa = 9.24WKK103 pKa = 11.25CFDD106 pKa = 4.41TDD108 pKa = 2.99NDD110 pKa = 3.7AA111 pKa = 5.3

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N3X0|A0A5B9N3X0_9CAUD Terminase OS=Aeromonas phage 4_L372D OX=2588518 GN=4L372D_130 PE=4 SV=1
MM1 pKa = 7.15QNKK4 pKa = 9.0KK5 pKa = 10.23VNIEE9 pKa = 4.02KK10 pKa = 10.55QLTVYY15 pKa = 10.06YY16 pKa = 9.58KK17 pKa = 10.29QEE19 pKa = 3.85STFYY23 pKa = 11.32VPDD26 pKa = 4.52ADD28 pKa = 4.52PRR30 pKa = 11.84RR31 pKa = 11.84LLAGRR36 pKa = 11.84LLRR39 pKa = 11.84RR40 pKa = 11.84YY41 pKa = 9.23KK42 pKa = 10.6KK43 pKa = 10.33KK44 pKa = 10.81NKK46 pKa = 9.9LKK48 pKa = 10.84

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

223

0

223

36422

35

858

163.3

18.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.898 ± 0.214

1.765 ± 0.099

6.2 ± 0.145

7.641 ± 0.236

4.558 ± 0.132

5.889 ± 0.238

1.894 ± 0.088

6.71 ± 0.14

8.742 ± 0.261

8.171 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.094

5.741 ± 0.14

2.858 ± 0.107

4.058 ± 0.122

3.819 ± 0.13

6.307 ± 0.18

5.307 ± 0.185

6.617 ± 0.158

1.436 ± 0.08

4.857 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski