Propionibacterium phage PAS50

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pahexavirus; Propionibacterium virus PAS50

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4MIL6|F4MIL6_9CAUD Gp26 OS=Propionibacterium phage PAS50 OX=504553 GN=PaP-PAS50_gp26 PE=4 SV=1
MM1 pKa = 6.8VTTILDD7 pKa = 4.19DD8 pKa = 3.98GTQTTVLQTVGTTTTAIITDD28 pKa = 3.88TEE30 pKa = 4.14NPEE33 pKa = 4.29TITAKK38 pKa = 9.63YY39 pKa = 8.23TISKK43 pKa = 10.27DD44 pKa = 3.17GTATYY49 pKa = 10.14SISGNTYY56 pKa = 10.46LGDD59 pKa = 3.57HH60 pKa = 5.01QHH62 pKa = 6.76IIKK65 pKa = 10.6LMYY68 pKa = 9.96DD69 pKa = 3.27YY70 pKa = 9.55CHH72 pKa = 6.45CVGRR76 pKa = 11.84FDD78 pKa = 3.87TTNTSNPDD86 pKa = 3.23NLDD89 pKa = 3.23NLFRR93 pKa = 11.84GG94 pKa = 3.91

Molecular weight: 10.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4MIK6|F4MIK6_9CAUD Gp15 OS=Propionibacterium phage PAS50 OX=504553 GN=PaP-PAS50_gp15 PE=4 SV=1
MM1 pKa = 7.85SGEE4 pKa = 3.79IASAYY9 pKa = 9.42VSLYY13 pKa = 10.38TKK15 pKa = 10.48IPGLKK20 pKa = 10.41ANVGKK25 pKa = 9.99QLSGVMPAEE34 pKa = 4.62GQRR37 pKa = 11.84SGSPFAKK44 pKa = 9.94GMKK47 pKa = 9.39LALGGAAMVGAINVAKK63 pKa = 10.41KK64 pKa = 10.12GLKK67 pKa = 9.86SIYY70 pKa = 10.1DD71 pKa = 3.46VTIGGGIARR80 pKa = 11.84AMAIDD85 pKa = 3.74EE86 pKa = 4.58AQAKK90 pKa = 8.36LTGLGHH96 pKa = 6.51TSSDD100 pKa = 3.06TSSIMNSAIEE110 pKa = 4.15AVTGTSYY117 pKa = 11.95ALGDD121 pKa = 3.87AASTAAALSASGVKK135 pKa = 10.18SGGQMTDD142 pKa = 2.69VLKK145 pKa = 10.01TVADD149 pKa = 3.5VSYY152 pKa = 10.98ISGKK156 pKa = 9.96SFQDD160 pKa = 2.85TGAIFTSVMARR171 pKa = 11.84GKK173 pKa = 10.44LQGDD177 pKa = 4.11DD178 pKa = 3.62MLQLTMAGVPVLSLLARR195 pKa = 11.84QTGKK199 pKa = 9.04TSAEE203 pKa = 4.02VSQMVSKK210 pKa = 10.7GQIDD214 pKa = 4.25FATFAAAMKK223 pKa = 10.35LGMGGAAQASGKK235 pKa = 7.48TFEE238 pKa = 4.62GAMKK242 pKa = 10.13NVKK245 pKa = 9.81GALGYY250 pKa = 10.89LGATAMAPFLNGLRR264 pKa = 11.84QIFVALNPVIKK275 pKa = 10.61SITDD279 pKa = 3.3SVKK282 pKa = 10.57PMFAAVDD289 pKa = 3.54AGIQRR294 pKa = 11.84MMPSILAWINRR305 pKa = 11.84MPAMITRR312 pKa = 11.84MNAQMRR318 pKa = 11.84AKK320 pKa = 10.11VEE322 pKa = 3.81QLKK325 pKa = 10.88GVFARR330 pKa = 11.84LHH332 pKa = 5.88LPVPKK337 pKa = 10.54VNLGAMFAGGTAVFGIVAAGVGKK360 pKa = 10.13LVAGFAPLAVSLKK373 pKa = 10.55NLLPSFGALKK383 pKa = 10.35GAAGGLGGVFRR394 pKa = 11.84ALGGPVGIVIGLFAAMFATNAQFRR418 pKa = 11.84AAVMQLVGVVGRR430 pKa = 11.84ALGQIMVAIQPLFGIVAGVVARR452 pKa = 11.84LAPVFGQIIGMVAGLAAQLVPVIGMLIARR481 pKa = 11.84LVPVITQIIGMVTQVAAMLLPMLMPVIQAVVAVIRR516 pKa = 11.84QVIGVIMQLVPVLMPVVQQILGAVMSVLPPIVGLIRR552 pKa = 11.84SLIPVIMSVMRR563 pKa = 11.84VVVQVVGAVLQVVARR578 pKa = 11.84IIPVVMPIYY587 pKa = 10.29VSVIGFIAKK596 pKa = 9.72IYY598 pKa = 9.49AAVIVFEE605 pKa = 4.58AKK607 pKa = 10.52VIGAILRR614 pKa = 11.84TITWIVNHH622 pKa = 6.49LVSGVRR628 pKa = 11.84SMGTAIQNGWNHH640 pKa = 5.97IKK642 pKa = 10.71SFTSAFINGFKK653 pKa = 10.57SVISGGVNAVVGFFTRR669 pKa = 11.84LGLSVASHH677 pKa = 5.49VRR679 pKa = 11.84SGFNAARR686 pKa = 11.84GAVSSAMNAIRR697 pKa = 11.84SVVSSVASAVGGFFSSMASRR717 pKa = 11.84VRR719 pKa = 11.84SGVVRR724 pKa = 11.84GFNGARR730 pKa = 11.84NAASSAMHH738 pKa = 6.4AMGSAVSSGVHH749 pKa = 5.04SVLGFFRR756 pKa = 11.84NLPGNIRR763 pKa = 11.84HH764 pKa = 6.5ALGNMGSLLVSAGRR778 pKa = 11.84DD779 pKa = 3.53VVAGLGNGIKK789 pKa = 10.29NALSGLLDD797 pKa = 3.49TVRR800 pKa = 11.84NMGSQVANAAKK811 pKa = 10.09SVLGIHH817 pKa = 5.93SPSRR821 pKa = 11.84VFRR824 pKa = 11.84DD825 pKa = 3.43EE826 pKa = 3.91VGRR829 pKa = 11.84QVVAGLAEE837 pKa = 5.4GITGNAGLALDD848 pKa = 4.45AMSGVAGRR856 pKa = 11.84LPDD859 pKa = 3.56VVDD862 pKa = 3.18ARR864 pKa = 11.84FGVRR868 pKa = 11.84SSVGSFTPYY877 pKa = 10.41DD878 pKa = 3.53RR879 pKa = 11.84YY880 pKa = 10.73RR881 pKa = 11.84RR882 pKa = 11.84ANEE885 pKa = 3.49KK886 pKa = 10.61SVVVNVNGPTYY897 pKa = 10.88GDD899 pKa = 3.46PNEE902 pKa = 3.83FAKK905 pKa = 10.67RR906 pKa = 11.84IEE908 pKa = 4.16RR909 pKa = 11.84QQRR912 pKa = 11.84DD913 pKa = 3.73ALNALAYY920 pKa = 9.98VV921 pKa = 4.0

Molecular weight: 94.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

8821

44

921

191.8

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.545 ± 0.572

1.372 ± 0.208

6.473 ± 0.517

4.637 ± 0.435

2.811 ± 0.205

8.65 ± 0.53

2.743 ± 0.284

4.682 ± 0.391

4.183 ± 0.321

7.346 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.562 ± 0.299

3.65 ± 0.25

5.113 ± 0.364

4.127 ± 0.269

6.224 ± 0.445

6.836 ± 0.308

6.428 ± 0.527

7.879 ± 0.699

2.029 ± 0.242

2.709 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski