Amino acid dipepetide frequency for Puniceispirillum phage HMO-2011

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.742AlaAla: 9.742 ± 1.427
0.646AlaCys: 0.646 ± 0.188
5.986AlaAsp: 5.986 ± 0.655
6.455AlaGlu: 6.455 ± 0.873
2.7AlaPhe: 2.7 ± 0.342
7.864AlaGly: 7.864 ± 0.616
0.939AlaHis: 0.939 ± 0.268
5.34AlaIle: 5.34 ± 0.606
6.69AlaLys: 6.69 ± 0.954
5.869AlaLeu: 5.869 ± 0.454
3.345AlaMet: 3.345 ± 0.53
5.223AlaAsn: 5.223 ± 0.659
2.817AlaPro: 2.817 ± 0.435
4.577AlaGln: 4.577 ± 0.873
4.93AlaArg: 4.93 ± 0.633
5.575AlaSer: 5.575 ± 0.68
7.688AlaThr: 7.688 ± 0.874
5.223AlaVal: 5.223 ± 0.531
1.056AlaTrp: 1.056 ± 0.24
2.7AlaTyr: 2.7 ± 0.349
0.0AlaXaa: 0.0 ± 0.0
Cys
0.763CysAla: 0.763 ± 0.236
0.059CysCys: 0.059 ± 0.052
0.352CysAsp: 0.352 ± 0.199
0.411CysGlu: 0.411 ± 0.165
0.117CysPhe: 0.117 ± 0.087
0.235CysGly: 0.235 ± 0.113
0.411CysHis: 0.411 ± 0.181
0.646CysIle: 0.646 ± 0.192
0.528CysLys: 0.528 ± 0.17
0.352CysLeu: 0.352 ± 0.15
0.117CysMet: 0.117 ± 0.074
0.528CysAsn: 0.528 ± 0.173
0.352CysPro: 0.352 ± 0.12
0.352CysGln: 0.352 ± 0.14
0.352CysArg: 0.352 ± 0.129
0.528CysSer: 0.528 ± 0.188
0.411CysThr: 0.411 ± 0.129
0.822CysVal: 0.822 ± 0.226
0.293CysTrp: 0.293 ± 0.121
0.176CysTyr: 0.176 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
6.573AspAla: 6.573 ± 0.681
0.469AspCys: 0.469 ± 0.153
4.519AspAsp: 4.519 ± 0.563
4.049AspGlu: 4.049 ± 0.595
2.817AspPhe: 2.817 ± 0.464
4.695AspGly: 4.695 ± 0.564
0.704AspHis: 0.704 ± 0.215
4.108AspIle: 4.108 ± 0.456
3.932AspLys: 3.932 ± 0.565
5.223AspLeu: 5.223 ± 0.588
2.23AspMet: 2.23 ± 0.388
2.934AspAsn: 2.934 ± 0.389
2.523AspPro: 2.523 ± 0.508
0.88AspGln: 0.88 ± 0.269
2.465AspArg: 2.465 ± 0.316
4.812AspSer: 4.812 ± 0.595
5.223AspThr: 5.223 ± 0.519
5.399AspVal: 5.399 ± 0.483
0.88AspTrp: 0.88 ± 0.215
3.169AspTyr: 3.169 ± 0.378
0.0AspXaa: 0.0 ± 0.0
Glu
6.749GluAla: 6.749 ± 0.747
0.352GluCys: 0.352 ± 0.153
3.873GluAsp: 3.873 ± 0.473
4.988GluGlu: 4.988 ± 0.897
1.937GluPhe: 1.937 ± 0.321
3.638GluGly: 3.638 ± 0.564
0.528GluHis: 0.528 ± 0.212
2.7GluIle: 2.7 ± 0.388
3.286GluLys: 3.286 ± 0.599
6.514GluLeu: 6.514 ± 0.489
1.995GluMet: 1.995 ± 0.42
1.702GluAsn: 1.702 ± 0.325
2.113GluPro: 2.113 ± 0.368
3.697GluGln: 3.697 ± 0.538
3.169GluArg: 3.169 ± 0.418
2.876GluSer: 2.876 ± 0.366
3.169GluThr: 3.169 ± 0.584
4.343GluVal: 4.343 ± 0.542
1.115GluTrp: 1.115 ± 0.285
2.347GluTyr: 2.347 ± 0.383
0.0GluXaa: 0.0 ± 0.0
Phe
3.345PheAla: 3.345 ± 0.51
0.352PheCys: 0.352 ± 0.142
3.11PheAsp: 3.11 ± 0.502
2.171PheGlu: 2.171 ± 0.338
0.763PhePhe: 0.763 ± 0.257
1.995PheGly: 1.995 ± 0.34
0.528PheHis: 0.528 ± 0.194
1.702PheIle: 1.702 ± 0.261
2.7PheLys: 2.7 ± 0.34
1.467PheLeu: 1.467 ± 0.246
1.291PheMet: 1.291 ± 0.264
2.465PheAsn: 2.465 ± 0.398
1.056PhePro: 1.056 ± 0.211
1.643PheGln: 1.643 ± 0.329
2.054PheArg: 2.054 ± 0.339
2.171PheSer: 2.171 ± 0.355
2.406PheThr: 2.406 ± 0.411
1.878PheVal: 1.878 ± 0.39
0.176PheTrp: 0.176 ± 0.098
1.056PheTyr: 1.056 ± 0.288
0.0PheXaa: 0.0 ± 0.0
Gly
4.871GlyAla: 4.871 ± 0.531
0.352GlyCys: 0.352 ± 0.136
5.692GlyAsp: 5.692 ± 0.612
3.756GlyGlu: 3.756 ± 0.456
3.052GlyPhe: 3.052 ± 0.389
6.221GlyGly: 6.221 ± 0.588
0.998GlyHis: 0.998 ± 0.262
4.519GlyIle: 4.519 ± 0.512
5.223GlyLys: 5.223 ± 0.666
5.047GlyLeu: 5.047 ± 0.52
2.876GlyMet: 2.876 ± 0.307
3.169GlyAsn: 3.169 ± 0.403
1.643GlyPro: 1.643 ± 0.31
2.934GlyGln: 2.934 ± 0.534
2.993GlyArg: 2.993 ± 0.413
5.458GlySer: 5.458 ± 0.748
6.749GlyThr: 6.749 ± 0.895
4.343GlyVal: 4.343 ± 0.442
0.822GlyTrp: 0.822 ± 0.229
2.347GlyTyr: 2.347 ± 0.316
0.0GlyXaa: 0.0 ± 0.0
His
1.291HisAla: 1.291 ± 0.315
0.176HisCys: 0.176 ± 0.107
1.291HisAsp: 1.291 ± 0.347
0.88HisGlu: 0.88 ± 0.322
0.235HisPhe: 0.235 ± 0.127
1.174HisGly: 1.174 ± 0.275
0.293HisHis: 0.293 ± 0.121
0.646HisIle: 0.646 ± 0.236
1.056HisLys: 1.056 ± 0.321
1.115HisLeu: 1.115 ± 0.233
0.411HisMet: 0.411 ± 0.132
0.88HisAsn: 0.88 ± 0.288
0.822HisPro: 0.822 ± 0.244
0.235HisGln: 0.235 ± 0.126
0.88HisArg: 0.88 ± 0.246
0.88HisSer: 0.88 ± 0.263
1.056HisThr: 1.056 ± 0.272
0.88HisVal: 0.88 ± 0.257
0.176HisTrp: 0.176 ± 0.083
0.528HisTyr: 0.528 ± 0.256
0.0HisXaa: 0.0 ± 0.0
Ile
4.754IleAla: 4.754 ± 0.514
0.469IleCys: 0.469 ± 0.158
4.754IleAsp: 4.754 ± 0.727
4.108IleGlu: 4.108 ± 0.525
0.704IlePhe: 0.704 ± 0.2
3.638IleGly: 3.638 ± 0.437
0.704IleHis: 0.704 ± 0.203
1.526IleIle: 1.526 ± 0.299
3.58IleLys: 3.58 ± 0.508
2.582IleLeu: 2.582 ± 0.383
1.702IleMet: 1.702 ± 0.332
2.876IleAsn: 2.876 ± 0.47
2.817IlePro: 2.817 ± 0.471
1.115IleGln: 1.115 ± 0.195
3.11IleArg: 3.11 ± 0.464
2.758IleSer: 2.758 ± 0.324
4.108IleThr: 4.108 ± 0.511
3.345IleVal: 3.345 ± 0.457
0.646IleTrp: 0.646 ± 0.19
1.174IleTyr: 1.174 ± 0.256
0.0IleXaa: 0.0 ± 0.0
Lys
7.277LysAla: 7.277 ± 1.208
0.411LysCys: 0.411 ± 0.155
3.169LysAsp: 3.169 ± 0.437
4.284LysGlu: 4.284 ± 0.462
2.347LysPhe: 2.347 ± 0.286
3.756LysGly: 3.756 ± 0.698
0.998LysHis: 0.998 ± 0.227
2.817LysIle: 2.817 ± 0.363
4.695LysLys: 4.695 ± 0.933
4.401LysLeu: 4.401 ± 0.502
2.113LysMet: 2.113 ± 0.432
2.406LysAsn: 2.406 ± 0.421
2.934LysPro: 2.934 ± 0.467
2.406LysGln: 2.406 ± 0.398
2.758LysArg: 2.758 ± 0.473
4.343LysSer: 4.343 ± 0.606
4.049LysThr: 4.049 ± 0.475
4.401LysVal: 4.401 ± 0.508
0.998LysTrp: 0.998 ± 0.223
1.819LysTyr: 1.819 ± 0.242
0.0LysXaa: 0.0 ± 0.0
Leu
6.573LeuAla: 6.573 ± 0.606
0.646LeuCys: 0.646 ± 0.174
6.221LeuAsp: 6.221 ± 0.551
4.754LeuGlu: 4.754 ± 0.485
2.289LeuPhe: 2.289 ± 0.352
4.049LeuGly: 4.049 ± 0.552
1.291LeuHis: 1.291 ± 0.4
3.345LeuIle: 3.345 ± 0.42
4.225LeuLys: 4.225 ± 0.5
4.695LeuLeu: 4.695 ± 0.644
2.171LeuMet: 2.171 ± 0.341
3.991LeuAsn: 3.991 ± 0.397
2.817LeuPro: 2.817 ± 0.425
2.7LeuGln: 2.7 ± 0.39
4.049LeuArg: 4.049 ± 0.531
5.164LeuSer: 5.164 ± 0.608
5.458LeuThr: 5.458 ± 0.775
3.638LeuVal: 3.638 ± 0.51
0.998LeuTrp: 0.998 ± 0.29
2.465LeuTyr: 2.465 ± 0.317
0.0LeuXaa: 0.0 ± 0.0
Met
4.225MetAla: 4.225 ± 0.576
0.293MetCys: 0.293 ± 0.128
2.23MetAsp: 2.23 ± 0.386
1.937MetGlu: 1.937 ± 0.402
0.469MetPhe: 0.469 ± 0.191
1.995MetGly: 1.995 ± 0.266
0.822MetHis: 0.822 ± 0.255
1.408MetIle: 1.408 ± 0.239
2.113MetLys: 2.113 ± 0.37
1.937MetLeu: 1.937 ± 0.371
0.704MetMet: 0.704 ± 0.207
1.585MetAsn: 1.585 ± 0.351
1.467MetPro: 1.467 ± 0.322
1.937MetGln: 1.937 ± 0.333
1.761MetArg: 1.761 ± 0.33
2.523MetSer: 2.523 ± 0.416
1.526MetThr: 1.526 ± 0.405
1.819MetVal: 1.819 ± 0.396
0.176MetTrp: 0.176 ± 0.119
0.704MetTyr: 0.704 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
4.284AsnAla: 4.284 ± 0.744
0.704AsnCys: 0.704 ± 0.269
2.113AsnAsp: 2.113 ± 0.339
2.758AsnGlu: 2.758 ± 0.407
1.467AsnPhe: 1.467 ± 0.288
3.697AsnGly: 3.697 ± 0.508
0.704AsnHis: 0.704 ± 0.197
2.641AsnIle: 2.641 ± 0.4
2.934AsnLys: 2.934 ± 0.505
3.991AsnLeu: 3.991 ± 0.539
1.761AsnMet: 1.761 ± 0.417
2.23AsnAsn: 2.23 ± 0.361
1.937AsnPro: 1.937 ± 0.38
1.702AsnGln: 1.702 ± 0.33
1.819AsnArg: 1.819 ± 0.387
3.404AsnSer: 3.404 ± 0.381
4.46AsnThr: 4.46 ± 0.568
2.876AsnVal: 2.876 ± 0.382
0.763AsnTrp: 0.763 ± 0.206
2.171AsnTyr: 2.171 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
3.345ProAla: 3.345 ± 0.532
0.293ProCys: 0.293 ± 0.124
2.817ProAsp: 2.817 ± 0.386
2.758ProGlu: 2.758 ± 0.357
1.995ProPhe: 1.995 ± 0.283
1.937ProGly: 1.937 ± 0.409
0.411ProHis: 0.411 ± 0.131
1.643ProIle: 1.643 ± 0.239
2.523ProLys: 2.523 ± 0.531
2.113ProLeu: 2.113 ± 0.295
1.115ProMet: 1.115 ± 0.275
1.819ProAsn: 1.819 ± 0.341
0.88ProPro: 0.88 ± 0.251
1.761ProGln: 1.761 ± 0.395
1.408ProArg: 1.408 ± 0.246
2.7ProSer: 2.7 ± 0.42
3.286ProThr: 3.286 ± 0.486
3.169ProVal: 3.169 ± 0.379
0.059ProTrp: 0.059 ± 0.056
1.643ProTyr: 1.643 ± 0.423
0.0ProXaa: 0.0 ± 0.0
Gln
3.638GlnAla: 3.638 ± 0.8
0.176GlnCys: 0.176 ± 0.102
2.582GlnAsp: 2.582 ± 0.403
2.7GlnGlu: 2.7 ± 0.425
1.878GlnPhe: 1.878 ± 0.355
2.054GlnGly: 2.054 ± 0.367
0.587GlnHis: 0.587 ± 0.211
1.819GlnIle: 1.819 ± 0.267
1.232GlnLys: 1.232 ± 0.342
3.873GlnLeu: 3.873 ± 0.594
0.998GlnMet: 0.998 ± 0.231
1.702GlnAsn: 1.702 ± 0.287
2.054GlnPro: 2.054 ± 0.388
2.523GlnGln: 2.523 ± 0.589
2.347GlnArg: 2.347 ± 0.391
3.462GlnSer: 3.462 ± 0.485
2.7GlnThr: 2.7 ± 0.48
2.876GlnVal: 2.876 ± 0.431
0.88GlnTrp: 0.88 ± 0.26
1.702GlnTyr: 1.702 ± 0.42
0.0GlnXaa: 0.0 ± 0.0
Arg
3.286ArgAla: 3.286 ± 0.465
0.293ArgCys: 0.293 ± 0.133
2.347ArgAsp: 2.347 ± 0.37
2.641ArgGlu: 2.641 ± 0.394
2.054ArgPhe: 2.054 ± 0.341
3.991ArgGly: 3.991 ± 0.481
1.115ArgHis: 1.115 ± 0.257
1.819ArgIle: 1.819 ± 0.337
3.873ArgLys: 3.873 ± 0.622
3.638ArgLeu: 3.638 ± 0.518
1.585ArgMet: 1.585 ± 0.283
2.641ArgAsn: 2.641 ± 0.415
1.643ArgPro: 1.643 ± 0.34
2.465ArgGln: 2.465 ± 0.405
3.169ArgArg: 3.169 ± 0.45
3.052ArgSer: 3.052 ± 0.442
2.993ArgThr: 2.993 ± 0.406
2.758ArgVal: 2.758 ± 0.385
0.646ArgTrp: 0.646 ± 0.155
2.523ArgTyr: 2.523 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
6.866SerAla: 6.866 ± 0.672
0.528SerCys: 0.528 ± 0.201
4.401SerAsp: 4.401 ± 0.51
2.582SerGlu: 2.582 ± 0.447
2.523SerPhe: 2.523 ± 0.371
6.984SerGly: 6.984 ± 0.674
0.939SerHis: 0.939 ± 0.282
3.521SerIle: 3.521 ± 0.471
3.756SerLys: 3.756 ± 0.573
5.282SerLeu: 5.282 ± 0.432
1.291SerMet: 1.291 ± 0.235
3.052SerAsn: 3.052 ± 0.518
2.406SerPro: 2.406 ± 0.405
3.462SerGln: 3.462 ± 0.54
3.228SerArg: 3.228 ± 0.467
4.636SerSer: 4.636 ± 0.71
5.223SerThr: 5.223 ± 0.591
4.636SerVal: 4.636 ± 0.478
0.411SerTrp: 0.411 ± 0.171
1.819SerTyr: 1.819 ± 0.353
0.0SerXaa: 0.0 ± 0.0
Thr
7.101ThrAla: 7.101 ± 0.911
0.528ThrCys: 0.528 ± 0.203
4.225ThrAsp: 4.225 ± 0.448
3.345ThrGlu: 3.345 ± 0.356
2.876ThrPhe: 2.876 ± 0.335
7.57ThrGly: 7.57 ± 0.818
0.528ThrHis: 0.528 ± 0.161
4.108ThrIle: 4.108 ± 0.531
3.991ThrLys: 3.991 ± 0.43
5.692ThrLeu: 5.692 ± 0.707
1.702ThrMet: 1.702 ± 0.369
3.815ThrAsn: 3.815 ± 0.516
3.052ThrPro: 3.052 ± 0.465
2.171ThrGln: 2.171 ± 0.298
2.289ThrArg: 2.289 ± 0.464
5.634ThrSer: 5.634 ± 0.63
4.519ThrThr: 4.519 ± 0.712
6.103ThrVal: 6.103 ± 0.658
0.822ThrTrp: 0.822 ± 0.241
2.817ThrTyr: 2.817 ± 0.439
0.0ThrXaa: 0.0 ± 0.0
Val
6.162ValAla: 6.162 ± 0.648
0.469ValCys: 0.469 ± 0.156
4.871ValAsp: 4.871 ± 0.597
4.049ValGlu: 4.049 ± 0.498
2.7ValPhe: 2.7 ± 0.459
3.991ValGly: 3.991 ± 0.507
1.291ValHis: 1.291 ± 0.367
3.932ValIle: 3.932 ± 0.466
3.228ValLys: 3.228 ± 0.493
4.049ValLeu: 4.049 ± 0.487
2.582ValMet: 2.582 ± 0.376
3.286ValAsn: 3.286 ± 0.455
2.582ValPro: 2.582 ± 0.37
2.993ValGln: 2.993 ± 0.527
3.169ValArg: 3.169 ± 0.461
4.812ValSer: 4.812 ± 0.556
5.164ValThr: 5.164 ± 0.556
4.401ValVal: 4.401 ± 0.554
0.939ValTrp: 0.939 ± 0.228
1.35ValTyr: 1.35 ± 0.243
0.0ValXaa: 0.0 ± 0.0
Trp
1.232TrpAla: 1.232 ± 0.255
0.352TrpCys: 0.352 ± 0.142
0.646TrpAsp: 0.646 ± 0.166
0.469TrpGlu: 0.469 ± 0.145
0.587TrpPhe: 0.587 ± 0.163
0.646TrpGly: 0.646 ± 0.186
0.352TrpHis: 0.352 ± 0.14
0.352TrpIle: 0.352 ± 0.151
0.411TrpLys: 0.411 ± 0.158
1.291TrpLeu: 1.291 ± 0.279
0.411TrpMet: 0.411 ± 0.141
0.587TrpAsn: 0.587 ± 0.182
0.235TrpPro: 0.235 ± 0.112
0.469TrpGln: 0.469 ± 0.151
0.763TrpArg: 0.763 ± 0.189
0.939TrpSer: 0.939 ± 0.233
0.763TrpThr: 0.763 ± 0.18
1.115TrpVal: 1.115 ± 0.21
0.176TrpTrp: 0.176 ± 0.105
0.763TrpTyr: 0.763 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.286TyrAla: 3.286 ± 0.43
0.235TyrCys: 0.235 ± 0.114
2.406TyrAsp: 2.406 ± 0.318
1.995TyrGlu: 1.995 ± 0.331
0.822TyrPhe: 0.822 ± 0.225
2.934TyrGly: 2.934 ± 0.446
0.763TyrHis: 0.763 ± 0.299
1.878TyrIle: 1.878 ± 0.368
2.406TyrLys: 2.406 ± 0.384
2.406TyrLeu: 2.406 ± 0.348
1.174TyrMet: 1.174 ± 0.231
1.526TyrAsn: 1.526 ± 0.283
1.526TyrPro: 1.526 ± 0.318
1.761TyrGln: 1.761 ± 0.398
1.819TyrArg: 1.819 ± 0.362
1.819TyrSer: 1.819 ± 0.29
1.937TyrThr: 1.937 ± 0.333
1.995TyrVal: 1.995 ± 0.292
0.587TyrTrp: 0.587 ± 0.187
1.115TyrTyr: 1.115 ± 0.26
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (17041 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski