Clostridium phage CDMH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Yongloolinvirus; Clostridioides virus CDMH1

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5JAK3|X5JAK3_9CAUD Uncharacterized protein OS=Clostridium phage CDMH1 OX=1411095 GN=CDHM1_gp74 PE=4 SV=1
MM1 pKa = 7.42SKK3 pKa = 10.56VIQCDD8 pKa = 3.5FCKK11 pKa = 10.69SIFEE15 pKa = 4.45EE16 pKa = 4.31NNLEE20 pKa = 4.84CIEE23 pKa = 4.36LYY25 pKa = 10.33KK26 pKa = 11.01KK27 pKa = 10.54NVEE30 pKa = 4.04NEE32 pKa = 3.95MINIDD37 pKa = 3.33KK38 pKa = 10.79HH39 pKa = 5.16MCPDD43 pKa = 3.78CYY45 pKa = 10.91EE46 pKa = 4.27KK47 pKa = 10.75FVGEE51 pKa = 4.1KK52 pKa = 10.14VEE54 pKa = 4.3KK55 pKa = 10.59KK56 pKa = 8.53ITNFEE61 pKa = 4.29KK62 pKa = 9.59ITRR65 pKa = 11.84DD66 pKa = 3.35KK67 pKa = 11.41EE68 pKa = 4.25SLKK71 pKa = 11.09DD72 pKa = 4.23FLFEE76 pKa = 5.06CDD78 pKa = 5.1AEE80 pKa = 4.63CSCCIYY86 pKa = 11.0ANKK89 pKa = 9.71DD90 pKa = 2.97DD91 pKa = 5.54CYY93 pKa = 10.04PTSCVTGCEE102 pKa = 3.66KK103 pKa = 10.28WLDD106 pKa = 3.79MEE108 pKa = 4.82VEE110 pKa = 4.21LL111 pKa = 5.83

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5JAI9|X5JAI9_9CAUD Putative minor head protein OS=Clostridium phage CDMH1 OX=1411095 GN=CDHM1_gp4 PE=4 SV=1
MM1 pKa = 7.22YY2 pKa = 9.29PPLKK6 pKa = 10.21RR7 pKa = 11.84LRR9 pKa = 11.84LTSRR13 pKa = 11.84SFLIQKK19 pKa = 8.22FIKK22 pKa = 9.76NARR25 pKa = 11.84HH26 pKa = 5.13ALFRR30 pKa = 11.84YY31 pKa = 9.34ACHH34 pKa = 6.78ACLKK38 pKa = 10.92SNII41 pKa = 3.98

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

15882

37

780

189.1

21.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.647 ± 0.419

1.467 ± 0.156

5.95 ± 0.225

8.437 ± 0.479

4.332 ± 0.234

5.15 ± 0.406

1.159 ± 0.097

9.023 ± 0.335

10.779 ± 0.411

8.569 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.132

7.253 ± 0.191

1.996 ± 0.132

2.789 ± 0.172

3.835 ± 0.224

6.114 ± 0.245

5.39 ± 0.339

5.465 ± 0.23

0.976 ± 0.106

4.319 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski