Enterobacter phage phiKDA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Koutsourovirus; Enterobacter virus KDA1

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A6Z5D2|A0A0A6Z5D2_9CAUD Putative DNA endonuclease VII OS=Enterobacter phage phiKDA1 OX=1147139 GN=phiKDA1_33 PE=4 SV=1
MM1 pKa = 7.76TDD3 pKa = 2.98TMIQEE8 pKa = 4.39ATPAIQFQNLSYY20 pKa = 10.25EE21 pKa = 4.12VSRR24 pKa = 11.84FIGDD28 pKa = 4.9LYY30 pKa = 11.0TDD32 pKa = 4.38MEE34 pKa = 4.54PMVDD38 pKa = 4.54GIKK41 pKa = 10.36SDD43 pKa = 3.91LAQLAKK49 pKa = 10.75LYY51 pKa = 10.77GADD54 pKa = 3.38LALVEE59 pKa = 4.8EE60 pKa = 5.21QMQQHH65 pKa = 6.23NDD67 pKa = 2.89IPAKK71 pKa = 10.43AEE73 pKa = 3.97KK74 pKa = 10.46LLEE77 pKa = 4.72DD78 pKa = 3.78PLVYY82 pKa = 10.53LRR84 pKa = 11.84IVGSLSAHH92 pKa = 7.08LGNIAAVLYY101 pKa = 10.56DD102 pKa = 3.81PEE104 pKa = 4.37QGLVAVEE111 pKa = 4.12VMGEE115 pKa = 3.92LLGTVLVLEE124 pKa = 4.46AAGYY128 pKa = 9.66

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A6Z5D3|A0A0A6Z5D3_9CAUD Putative DNA methylase OS=Enterobacter phage phiKDA1 OX=1147139 GN=phiKDA1_38 PE=4 SV=1
MM1 pKa = 6.95TLRR4 pKa = 11.84RR5 pKa = 11.84ITRR8 pKa = 11.84AQIRR12 pKa = 11.84SVAMKK17 pKa = 10.01LAKK20 pKa = 9.87EE21 pKa = 3.91QGGACLLCGGALDD34 pKa = 4.68FSIKK38 pKa = 9.88GVKK41 pKa = 9.6GDD43 pKa = 3.8SVVVDD48 pKa = 3.75HH49 pKa = 6.3CHH51 pKa = 4.28ITGRR55 pKa = 11.84IRR57 pKa = 11.84GALHH61 pKa = 6.7RR62 pKa = 11.84SCNGGEE68 pKa = 4.21GKK70 pKa = 10.14VASAAGRR77 pKa = 11.84WIVGSMQSSAAIAAALRR94 pKa = 11.84RR95 pKa = 11.84VADD98 pKa = 3.68YY99 pKa = 11.23LDD101 pKa = 4.43RR102 pKa = 11.84EE103 pKa = 4.58PTQLLYY109 pKa = 9.2YY110 pKa = 8.18TWKK113 pKa = 9.39TEE115 pKa = 4.08EE116 pKa = 3.98EE117 pKa = 3.93AKK119 pKa = 10.45AAQNAKK125 pKa = 9.33RR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84VRR130 pKa = 11.84AQRR133 pKa = 11.84KK134 pKa = 7.6ARR136 pKa = 11.84EE137 pKa = 4.12VIKK140 pKa = 11.06NGG142 pKa = 3.32

Molecular weight: 15.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13675

38

1236

220.6

24.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.801 ± 0.457

1.397 ± 0.219

5.821 ± 0.248

5.777 ± 0.319

2.815 ± 0.207

7.422 ± 0.288

1.623 ± 0.145

4.278 ± 0.236

5.177 ± 0.264

9.024 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.984 ± 0.219

3.912 ± 0.222

4.037 ± 0.243

5.148 ± 0.355

5.426 ± 0.284

6.274 ± 0.283

5.909 ± 0.431

6.633 ± 0.206

1.448 ± 0.103

4.095 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski