Amino acid dipepetide frequency for Prevotellaceae bacterium KH2P17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.785AlaAla: 7.785 ± 0.113	1.134AlaCys: 1.134 ± 0.039	5.48AlaAsp: 5.48 ± 0.085	5.16AlaGlu: 5.16 ± 0.084	3.22AlaPhe: 3.22 ± 0.055	6.152AlaGly: 6.152 ± 0.09	1.549AlaHis: 1.549 ± 0.046	4.485AlaIle: 4.485 ± 0.087	4.137AlaLys: 4.137 ± 0.068	7.241AlaLeu: 7.241 ± 0.102	2.195AlaMet: 2.195 ± 0.056	3.501AlaAsn: 3.501 ± 0.063	2.631AlaPro: 2.631 ± 0.059	3.203AlaGln: 3.203 ± 0.059	4.289AlaArg: 4.289 ± 0.08	4.508AlaSer: 4.508 ± 0.082	4.428AlaThr: 4.428 ± 0.07	5.747AlaVal: 5.747 ± 0.084	0.955AlaTrp: 0.955 ± 0.038	3.229AlaTyr: 3.229 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.837CysAla: 0.837 ± 0.03	0.206CysCys: 0.206 ± 0.015	0.674CysAsp: 0.674 ± 0.027	0.58CysGlu: 0.58 ± 0.024	0.534CysPhe: 0.534 ± 0.023	1.043CysGly: 1.043 ± 0.039	0.341CysHis: 0.341 ± 0.02	0.796CysIle: 0.796 ± 0.03	0.61CysLys: 0.61 ± 0.025	1.12CysLeu: 1.12 ± 0.041	0.332CysMet: 0.332 ± 0.02	0.576CysAsn: 0.576 ± 0.025	0.505CysPro: 0.505 ± 0.026	0.38CysGln: 0.38 ± 0.02	0.792CysArg: 0.792 ± 0.032	0.8CysSer: 0.8 ± 0.03	0.681CysThr: 0.681 ± 0.03	0.784CysVal: 0.784 ± 0.031	0.181CysTrp: 0.181 ± 0.012	0.499CysTyr: 0.499 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	4.699AspAla: 4.699 ± 0.074	0.691AspCys: 0.691 ± 0.027	3.109AspAsp: 3.109 ± 0.064	4.126AspGlu: 4.126 ± 0.084	2.952AspPhe: 2.952 ± 0.061	4.61AspGly: 4.61 ± 0.085	0.958AspHis: 0.958 ± 0.03	3.975AspIle: 3.975 ± 0.068	3.638AspLys: 3.638 ± 0.076	4.379AspLeu: 4.379 ± 0.065	1.678AspMet: 1.678 ± 0.043	2.826AspAsn: 2.826 ± 0.05	1.855AspPro: 1.855 ± 0.046	1.303AspGln: 1.303 ± 0.035	2.922AspArg: 2.922 ± 0.058	3.134AspSer: 3.134 ± 0.067	2.78AspThr: 2.78 ± 0.059	3.806AspVal: 3.806 ± 0.066	0.966AspTrp: 0.966 ± 0.034	2.987AspTyr: 2.987 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	5.175GluAla: 5.175 ± 0.09	0.578GluCys: 0.578 ± 0.023	2.965GluAsp: 2.965 ± 0.065	3.989GluGlu: 3.989 ± 0.094	2.221GluPhe: 2.221 ± 0.047	3.91GluGly: 3.91 ± 0.068	1.377GluHis: 1.377 ± 0.043	3.659GluIle: 3.659 ± 0.069	3.846GluLys: 3.846 ± 0.089	5.639GluLeu: 5.639 ± 0.095	1.8GluMet: 1.8 ± 0.048	2.795GluAsn: 2.795 ± 0.061	1.984GluPro: 1.984 ± 0.05	2.87GluGln: 2.87 ± 0.062	3.427GluArg: 3.427 ± 0.066	2.699GluSer: 2.699 ± 0.049	3.192GluThr: 3.192 ± 0.063	3.822GluVal: 3.822 ± 0.062	0.778GluTrp: 0.778 ± 0.031	2.372GluTyr: 2.372 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.233PheAla: 3.233 ± 0.061	0.684PheCys: 0.684 ± 0.025	2.791PheAsp: 2.791 ± 0.054	2.165PheGlu: 2.165 ± 0.048	1.936PhePhe: 1.936 ± 0.053	3.434PheGly: 3.434 ± 0.066	0.862PheHis: 0.862 ± 0.034	2.463PheIle: 2.463 ± 0.052	1.978PheLys: 1.978 ± 0.049	3.548PheLeu: 3.548 ± 0.069	1.257PheMet: 1.257 ± 0.039	2.168PheAsn: 2.168 ± 0.051	1.566PhePro: 1.566 ± 0.041	1.097PheGln: 1.097 ± 0.029	2.399PheArg: 2.399 ± 0.052	3.181PheSer: 3.181 ± 0.06	2.748PheThr: 2.748 ± 0.057	2.85PheVal: 2.85 ± 0.065	0.546PheTrp: 0.546 ± 0.027	1.878PheTyr: 1.878 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.969GlyAla: 4.969 ± 0.086	0.934GlyCys: 0.934 ± 0.033	3.638GlyAsp: 3.638 ± 0.06	3.934GlyGlu: 3.934 ± 0.071	3.19GlyPhe: 3.19 ± 0.063	5.165GlyGly: 5.165 ± 0.094	1.569GlyHis: 1.569 ± 0.042	4.88GlyIle: 4.88 ± 0.082	4.701GlyLys: 4.701 ± 0.071	6.126GlyLeu: 6.126 ± 0.092	2.124GlyMet: 2.124 ± 0.053	3.682GlyAsn: 3.682 ± 0.065	1.46GlyPro: 1.46 ± 0.046	2.729GlyGln: 2.729 ± 0.051	4.146GlyArg: 4.146 ± 0.072	4.6GlySer: 4.6 ± 0.084	4.565GlyThr: 4.565 ± 0.084	4.989GlyVal: 4.989 ± 0.088	1.118GlyTrp: 1.118 ± 0.042	3.406GlyTyr: 3.406 ± 0.075	0.0GlyXaa: 0.0 ± 0.0
His	1.51HisAla: 1.51 ± 0.046	0.336HisCys: 0.336 ± 0.02	1.148HisAsp: 1.148 ± 0.036	1.178HisGlu: 1.178 ± 0.03	0.999HisPhe: 0.999 ± 0.034	1.517HisGly: 1.517 ± 0.043	0.615HisHis: 0.615 ± 0.029	1.38HisIle: 1.38 ± 0.042	0.995HisLys: 0.995 ± 0.03	1.914HisLeu: 1.914 ± 0.045	0.421HisMet: 0.421 ± 0.019	1.011HisAsn: 1.011 ± 0.037	1.183HisPro: 1.183 ± 0.039	0.726HisGln: 0.726 ± 0.025	1.168HisArg: 1.168 ± 0.034	1.144HisSer: 1.144 ± 0.038	1.123HisThr: 1.123 ± 0.035	1.386HisVal: 1.386 ± 0.038	0.299HisTrp: 0.299 ± 0.018	0.938HisTyr: 0.938 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	4.985IleAla: 4.985 ± 0.088	0.78IleCys: 0.78 ± 0.032	4.116IleAsp: 4.116 ± 0.076	3.6IleGlu: 3.6 ± 0.071	2.227IlePhe: 2.227 ± 0.055	4.373IleGly: 4.373 ± 0.075	1.196IleHis: 1.196 ± 0.035	3.722IleIle: 3.722 ± 0.074	3.162IleLys: 3.162 ± 0.062	4.781IleLeu: 4.781 ± 0.081	1.388IleMet: 1.388 ± 0.043	2.951IleAsn: 2.951 ± 0.054	2.663IlePro: 2.663 ± 0.049	1.756IleGln: 1.756 ± 0.045	3.362IleArg: 3.362 ± 0.069	3.857IleSer: 3.857 ± 0.071	3.698IleThr: 3.698 ± 0.067	4.046IleVal: 4.046 ± 0.07	0.56IleTrp: 0.56 ± 0.025	2.378IleTyr: 2.378 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.924LysAla: 4.924 ± 0.089	0.492LysCys: 0.492 ± 0.023	3.447LysAsp: 3.447 ± 0.066	4.001LysGlu: 4.001 ± 0.087	2.001LysPhe: 2.001 ± 0.042	3.927LysGly: 3.927 ± 0.065	1.137LysHis: 1.137 ± 0.037	3.222LysIle: 3.222 ± 0.071	3.703LysLys: 3.703 ± 0.077	4.96LysLeu: 4.96 ± 0.07	1.779LysMet: 1.779 ± 0.046	2.673LysAsn: 2.673 ± 0.072	2.29LysPro: 2.29 ± 0.048	2.445LysGln: 2.445 ± 0.06	2.903LysArg: 2.903 ± 0.068	2.937LysSer: 2.937 ± 0.059	3.31LysThr: 3.31 ± 0.066	3.836LysVal: 3.836 ± 0.067	0.76LysTrp: 0.76 ± 0.029	2.503LysTyr: 2.503 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	7.183LeuAla: 7.183 ± 0.103	1.391LeuCys: 1.391 ± 0.04	4.793LeuAsp: 4.793 ± 0.078	4.295LeuGlu: 4.295 ± 0.084	3.979LeuPhe: 3.979 ± 0.085	5.745LeuGly: 5.745 ± 0.1	2.046LeuHis: 2.046 ± 0.049	4.647LeuIle: 4.647 ± 0.077	5.38LeuLys: 5.38 ± 0.079	9.056LeuLeu: 9.056 ± 0.139	2.737LeuMet: 2.737 ± 0.059	4.306LeuAsn: 4.306 ± 0.079	4.185LeuPro: 4.185 ± 0.067	3.842LeuGln: 3.842 ± 0.075	5.248LeuArg: 5.248 ± 0.076	6.187LeuSer: 6.187 ± 0.083	5.585LeuThr: 5.585 ± 0.075	5.225LeuVal: 5.225 ± 0.089	1.046LeuTrp: 1.046 ± 0.034	3.571LeuTyr: 3.571 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.565MetAla: 2.565 ± 0.055	0.21MetCys: 0.21 ± 0.016	1.589MetAsp: 1.589 ± 0.046	1.793MetGlu: 1.793 ± 0.044	0.925MetPhe: 0.925 ± 0.036	1.914MetGly: 1.914 ± 0.05	0.497MetHis: 0.497 ± 0.024	1.325MetIle: 1.325 ± 0.038	2.355MetLys: 2.355 ± 0.051	2.641MetLeu: 2.641 ± 0.064	0.931MetMet: 0.931 ± 0.035	1.509MetAsn: 1.509 ± 0.039	1.259MetPro: 1.259 ± 0.039	1.234MetGln: 1.234 ± 0.038	1.425MetArg: 1.425 ± 0.042	1.609MetSer: 1.609 ± 0.043	1.564MetThr: 1.564 ± 0.037	1.629MetVal: 1.629 ± 0.042	0.251MetTrp: 0.251 ± 0.016	0.819MetTyr: 0.819 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.702AsnAla: 3.702 ± 0.069	0.506AsnCys: 0.506 ± 0.023	2.667AsnAsp: 2.667 ± 0.057	2.614AsnGlu: 2.614 ± 0.054	2.022AsnPhe: 2.022 ± 0.048	3.979AsnGly: 3.979 ± 0.081	0.976AsnHis: 0.976 ± 0.03	3.283AsnIle: 3.283 ± 0.062	2.666AsnLys: 2.666 ± 0.065	4.134AsnLeu: 4.134 ± 0.07	1.236AsnMet: 1.236 ± 0.037	2.275AsnAsn: 2.275 ± 0.061	2.364AsnPro: 2.364 ± 0.054	1.567AsnGln: 1.567 ± 0.048	2.59AsnArg: 2.59 ± 0.056	2.647AsnSer: 2.647 ± 0.063	2.586AsnThr: 2.586 ± 0.06	3.245AsnVal: 3.245 ± 0.061	0.663AsnTrp: 0.663 ± 0.029	2.231AsnTyr: 2.231 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	3.542ProAla: 3.542 ± 0.064	0.366ProCys: 0.366 ± 0.021	2.563ProAsp: 2.563 ± 0.058	2.981ProGlu: 2.981 ± 0.062	1.801ProPhe: 1.801 ± 0.041	2.584ProGly: 2.584 ± 0.054	0.727ProHis: 0.727 ± 0.026	1.998ProIle: 1.998 ± 0.046	1.884ProLys: 1.884 ± 0.042	3.461ProLeu: 3.461 ± 0.054	1.04ProMet: 1.04 ± 0.033	1.632ProAsn: 1.632 ± 0.039	0.832ProPro: 0.832 ± 0.029	1.742ProGln: 1.742 ± 0.049	1.674ProArg: 1.674 ± 0.047	2.124ProSer: 2.124 ± 0.044	2.169ProThr: 2.169 ± 0.053	3.039ProVal: 3.039 ± 0.059	0.509ProTrp: 0.509 ± 0.024	1.761ProTyr: 1.761 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.211GlnAla: 3.211 ± 0.058	0.355GlnCys: 0.355 ± 0.018	1.735GlnAsp: 1.735 ± 0.047	2.311GlnGlu: 2.311 ± 0.064	1.471GlnPhe: 1.471 ± 0.04	2.499GlnGly: 2.499 ± 0.061	0.854GlnHis: 0.854 ± 0.032	2.149GlnIle: 2.149 ± 0.046	2.306GlnLys: 2.306 ± 0.051	3.913GlnLeu: 3.913 ± 0.08	1.133GlnMet: 1.133 ± 0.035	1.778GlnAsn: 1.778 ± 0.046	1.661GlnPro: 1.661 ± 0.043	2.265GlnGln: 2.265 ± 0.062	2.054GlnArg: 2.054 ± 0.049	2.096GlnSer: 2.096 ± 0.05	2.261GlnThr: 2.261 ± 0.049	2.371GlnVal: 2.371 ± 0.048	0.528GlnTrp: 0.528 ± 0.024	1.591GlnTyr: 1.591 ± 0.044	0.0GlnXaa: 0.0 ± 0.0
Arg	3.458ArgAla: 3.458 ± 0.068	0.582ArgCys: 0.582 ± 0.027	2.593ArgAsp: 2.593 ± 0.058	3.374ArgGlu: 3.374 ± 0.073	2.409ArgPhe: 2.409 ± 0.052	3.073ArgGly: 3.073 ± 0.052	1.468ArgHis: 1.468 ± 0.047	3.447ArgIle: 3.447 ± 0.06	3.457ArgLys: 3.457 ± 0.064	5.371ArgLeu: 5.371 ± 0.078	1.812ArgMet: 1.812 ± 0.045	2.752ArgAsn: 2.752 ± 0.067	2.009ArgPro: 2.009 ± 0.053	2.91ArgGln: 2.91 ± 0.059	3.566ArgArg: 3.566 ± 0.065	3.0ArgSer: 3.0 ± 0.063	2.838ArgThr: 2.838 ± 0.056	3.02ArgVal: 3.02 ± 0.062	0.768ArgTrp: 0.768 ± 0.028	2.62ArgTyr: 2.62 ± 0.06	0.0ArgXaa: 0.0 ± 0.0
Ser	4.733SerAla: 4.733 ± 0.076	0.773SerCys: 0.773 ± 0.033	3.071SerAsp: 3.071 ± 0.055	3.155SerGlu: 3.155 ± 0.061	2.934SerPhe: 2.934 ± 0.059	4.519SerGly: 4.519 ± 0.084	1.342SerHis: 1.342 ± 0.041	3.575SerIle: 3.575 ± 0.064	3.009SerLys: 3.009 ± 0.068	5.631SerLeu: 5.631 ± 0.084	1.506SerMet: 1.506 ± 0.042	2.661SerAsn: 2.661 ± 0.059	2.364SerPro: 2.364 ± 0.044	2.019SerGln: 2.019 ± 0.048	3.151SerArg: 3.151 ± 0.056	3.543SerSer: 3.543 ± 0.072	3.12SerThr: 3.12 ± 0.065	4.213SerVal: 4.213 ± 0.072	0.873SerTrp: 0.873 ± 0.032	2.925SerTyr: 2.925 ± 0.07	0.0SerXaa: 0.0 ± 0.0
Thr	5.083ThrAla: 5.083 ± 0.076	0.615ThrCys: 0.615 ± 0.029	3.778ThrAsp: 3.778 ± 0.069	3.11ThrGlu: 3.11 ± 0.059	2.578ThrPhe: 2.578 ± 0.056	4.563ThrGly: 4.563 ± 0.083	1.101ThrHis: 1.101 ± 0.038	3.441ThrIle: 3.441 ± 0.056	2.589ThrLys: 2.589 ± 0.053	5.6ThrLeu: 5.6 ± 0.09	1.322ThrMet: 1.322 ± 0.036	2.441ThrAsn: 2.441 ± 0.059	2.774ThrPro: 2.774 ± 0.062	1.886ThrGln: 1.886 ± 0.038	2.571ThrArg: 2.571 ± 0.061	3.117ThrSer: 3.117 ± 0.063	3.405ThrThr: 3.405 ± 0.073	4.305ThrVal: 4.305 ± 0.078	0.69ThrTrp: 0.69 ± 0.027	2.505ThrTyr: 2.505 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	5.296ValAla: 5.296 ± 0.105	0.951ValCys: 0.951 ± 0.03	3.847ValAsp: 3.847 ± 0.07	3.733ValGlu: 3.733 ± 0.079	2.854ValPhe: 2.854 ± 0.069	4.586ValGly: 4.586 ± 0.079	1.114ValHis: 1.114 ± 0.037	3.93ValIle: 3.93 ± 0.068	3.787ValLys: 3.787 ± 0.07	5.843ValLeu: 5.843 ± 0.097	1.814ValMet: 1.814 ± 0.043	3.296ValAsn: 3.296 ± 0.065	2.715ValPro: 2.715 ± 0.059	2.205ValGln: 2.205 ± 0.049	3.521ValArg: 3.521 ± 0.066	4.611ValSer: 4.611 ± 0.072	3.99ValThr: 3.99 ± 0.079	4.891ValVal: 4.891 ± 0.083	0.807ValTrp: 0.807 ± 0.031	2.712ValTyr: 2.712 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.861TrpAla: 0.861 ± 0.031	0.176TrpCys: 0.176 ± 0.013	0.709TrpAsp: 0.709 ± 0.028	0.665TrpGlu: 0.665 ± 0.028	0.575TrpPhe: 0.575 ± 0.027	0.957TrpGly: 0.957 ± 0.035	0.321TrpHis: 0.321 ± 0.019	0.711TrpIle: 0.711 ± 0.027	0.808TrpLys: 0.808 ± 0.03	1.305TrpLeu: 1.305 ± 0.043	0.427TrpMet: 0.427 ± 0.022	0.803TrpAsn: 0.803 ± 0.031	0.369TrpPro: 0.369 ± 0.019	0.717TrpGln: 0.717 ± 0.031	0.711TrpArg: 0.711 ± 0.029	0.726TrpSer: 0.726 ± 0.027	0.782TrpThr: 0.782 ± 0.03	0.702TrpVal: 0.702 ± 0.025	0.226TrpTrp: 0.226 ± 0.016	0.511TrpTyr: 0.511 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.276TyrAla: 3.276 ± 0.065	0.511TyrCys: 0.511 ± 0.023	2.678TyrAsp: 2.678 ± 0.064	2.332TyrGlu: 2.332 ± 0.058	1.957TyrPhe: 1.957 ± 0.043	3.375TyrGly: 3.375 ± 0.075	0.943TyrHis: 0.943 ± 0.035	2.514TyrIle: 2.514 ± 0.05	2.236TyrLys: 2.236 ± 0.053	3.783TyrLeu: 3.783 ± 0.06	1.112TyrMet: 1.112 ± 0.039	2.32TyrAsn: 2.32 ± 0.062	1.757TyrPro: 1.757 ± 0.046	1.648TyrGln: 1.648 ± 0.049	2.609TyrArg: 2.609 ± 0.056	2.597TyrSer: 2.597 ± 0.06	2.648TyrThr: 2.648 ± 0.065	2.6TyrVal: 2.6 ± 0.061	0.54TyrTrp: 0.54 ± 0.023	2.124TyrTyr: 2.124 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2620 proteins (960076 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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