Rickettsiaceae bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiaceae; unclassified Rickettsiaceae

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q3DDD1|A0A4Q3DDD1_9RICK RNA-directed DNA polymerase (Fragment) OS=Rickettsiaceae bacterium OX=2023360 GN=EOP33_09545 PE=4 SV=1
MM1 pKa = 7.09QLFAGFVEE9 pKa = 5.48DD10 pKa = 4.38YY11 pKa = 10.82PMSYY15 pKa = 10.57VFNDD19 pKa = 3.48DD20 pKa = 4.21VEE22 pKa = 5.36DD23 pKa = 3.93EE24 pKa = 4.31EE25 pKa = 6.15HH26 pKa = 6.58FFEE29 pKa = 5.31SLGDD33 pKa = 4.03PIYY36 pKa = 10.91DD37 pKa = 3.57EE38 pKa = 5.02EE39 pKa = 6.49LDD41 pKa = 4.1DD42 pKa = 5.78EE43 pKa = 4.59EE44 pKa = 6.08FNDD47 pKa = 3.46EE48 pKa = 6.12SIFQKK53 pKa = 10.98YY54 pKa = 9.74NIRR57 pKa = 11.84EE58 pKa = 4.23PIFDD62 pKa = 3.6YY63 pKa = 10.64PIFEE67 pKa = 4.37EE68 pKa = 5.51LIFDD72 pKa = 4.94KK73 pKa = 11.28DD74 pKa = 3.73EE75 pKa = 4.48EE76 pKa = 4.25DD77 pKa = 3.56TKK79 pKa = 11.12ISFSPIINQSDD90 pKa = 3.5GGGTIFVDD98 pKa = 3.63VSYY101 pKa = 10.87LFEE104 pKa = 4.98VEE106 pKa = 4.68LSILEE111 pKa = 4.39FIKK114 pKa = 10.61IVILYY119 pKa = 8.97SHH121 pKa = 6.85HH122 pKa = 5.65TT123 pKa = 3.54

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q3DHK2|A0A4Q3DHK2_9RICK Uncharacterized protein OS=Rickettsiaceae bacterium OX=2023360 GN=EOP33_06405 PE=4 SV=1
MM1 pKa = 7.37TPPARR6 pKa = 11.84RR7 pKa = 11.84VRR9 pKa = 11.84AGRR12 pKa = 11.84AGSVAAAPAFGKK24 pKa = 8.3TAARR28 pKa = 11.84AAALPAAAPAPARR41 pKa = 11.84PAAPPAAPPAPARR54 pKa = 11.84WRR56 pKa = 11.84PANLRR61 pKa = 11.84FPPMSCAAPAGRR73 pKa = 11.84AGPGRR78 pKa = 11.84ARR80 pKa = 11.84WWW82 pKa = 3.33

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1803

0

1803

516923

18

2734

286.7

32.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.938 ± 0.077

1.136 ± 0.023

4.85 ± 0.05

5.412 ± 0.073

4.872 ± 0.061

5.746 ± 0.071

2.091 ± 0.028

8.944 ± 0.084

7.082 ± 0.08

10.278 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.027

5.782 ± 0.066

3.526 ± 0.046

3.993 ± 0.052

4.033 ± 0.058

7.287 ± 0.052

5.253 ± 0.043

5.994 ± 0.05

0.973 ± 0.023

3.562 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski