Streptococcus phage Javan383
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A906|A0A4D6A906_9CAUD Uncharacterized protein OS=Streptococcus phage Javan383 OX=2548139 GN=Javan383_0016 PE=4 SV=1
MM1 pKa = 7.79 IIDD4 pKa = 3.31 IVDD7 pKa = 3.66 YY8 pKa = 10.63 KK9 pKa = 10.91 DD10 pKa = 3.23 EE11 pKa = 4.12 RR12 pKa = 11.84 TKK14 pKa = 11.09 VDD16 pKa = 3.24 IGDD19 pKa = 4.13 IEE21 pKa = 4.9 NIDD24 pKa = 4.16 YY25 pKa = 10.82 IHH27 pKa = 6.76 VLILSGDD34 pKa = 3.86 EE35 pKa = 5.9 VITAWYY41 pKa = 10.1 KK42 pKa = 11.1 DD43 pKa = 3.37 GTYY46 pKa = 10.8 DD47 pKa = 3.81 DD48 pKa = 4.74 WDD50 pKa = 3.78 SSVYY54 pKa = 10.54 RR55 pKa = 11.84 GTDD58 pKa = 3.41 YY59 pKa = 11.64 YY60 pKa = 11.4 DD61 pKa = 3.6 GDD63 pKa = 4.15 YY64 pKa = 10.62 EE65 pKa = 4.54 VPSEE69 pKa = 4.81 QIEE72 pKa = 4.52 KK73 pKa = 8.66 WSSMPGSSYY82 pKa = 11.34 DD83 pKa = 3.14 RR84 pKa = 11.84 MEE86 pKa = 3.83 IFIEE90 pKa = 4.2 SEE92 pKa = 3.92 EE93 pKa = 4.29 VEE95 pKa = 4.22
Molecular weight: 11.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.516
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.567
EMBOSS 3.668
Sillero 3.846
Patrickios 0.947
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A4D6AA47|A0A4D6AA47_9CAUD Uncharacterized protein OS=Streptococcus phage Javan383 OX=2548139 GN=Javan383_0045 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 9.86 IDD4 pKa = 3.76 VNGLPVLEE12 pKa = 4.17 ARR14 pKa = 11.84 FRR16 pKa = 11.84 AMNEE20 pKa = 4.0 IQWEE24 pKa = 4.14 AVVNKK29 pKa = 10.36 NLTTIFNRR37 pKa = 11.84 ASRR40 pKa = 11.84 PPGTPIGKK48 pKa = 9.11 QSKK51 pKa = 8.74 GHH53 pKa = 5.84 SAGEE57 pKa = 3.92 LMKK60 pKa = 10.55 SRR62 pKa = 11.84 RR63 pKa = 11.84 QIEE66 pKa = 4.44 LNSGAGVAIGMFYY79 pKa = 10.6 YY80 pKa = 10.78 SKK82 pKa = 10.82 DD83 pKa = 3.58 YY84 pKa = 10.74 GPHH87 pKa = 5.64 VEE89 pKa = 4.06 YY90 pKa = 10.46 GHH92 pKa = 6.8 RR93 pKa = 11.84 IVRR96 pKa = 11.84 GGRR99 pKa = 11.84 QVGYY103 pKa = 10.86 VSGQRR108 pKa = 11.84 FLFNNVQKK116 pKa = 11.01 QRR118 pKa = 11.84 MTYY121 pKa = 10.13 RR122 pKa = 11.84 NDD124 pKa = 3.13 MLNEE128 pKa = 3.85 LRR130 pKa = 11.84 KK131 pKa = 10.04 KK132 pKa = 10.45 RR133 pKa = 3.7
Molecular weight: 15.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.897
IPC_protein 10.657
Toseland 10.701
ProMoST 10.57
Dawson 10.818
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.096
Grimsley 10.877
Solomon 10.906
Lehninger 10.877
Nozaki 10.672
DTASelect 10.526
Thurlkill 10.716
EMBOSS 11.096
Sillero 10.745
Patrickios 10.818
IPC_peptide 10.921
IPC2_peptide 9.311
IPC2.peptide.svr19 8.552
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58
0
58
10550
40
984
181.9
20.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.332 ± 0.401
0.483 ± 0.083
6.313 ± 0.331
7.185 ± 0.453
4.237 ± 0.244
6.749 ± 0.474
1.261 ± 0.132
7.82 ± 0.353
8.616 ± 0.442
8.057 ± 0.252
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.217
6.104 ± 0.219
2.72 ± 0.148
3.735 ± 0.242
3.498 ± 0.302
6.142 ± 0.418
6.36 ± 0.474
6.256 ± 0.187
1.242 ± 0.157
4.322 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here