Methanoplanus limicola DSM 2279

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanomicrobiaceae; Methanoplanus; Methanoplanus limicola

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H1Z156|H1Z156_9EURY Putative nickel-responsive regulator OS=Methanoplanus limicola DSM 2279 OX=937775 GN=Metlim_1213 PE=3 SV=1
MM1 pKa = 7.76LFFTPVTASPSSGDD15 pKa = 3.58LTDD18 pKa = 3.74AGYY21 pKa = 10.85VALEE25 pKa = 4.33SGDD28 pKa = 3.34NRR30 pKa = 11.84MAKK33 pKa = 9.02TLFTRR38 pKa = 11.84ALDD41 pKa = 3.59TGGQNSPLAWAGLGKK56 pKa = 10.38ALWNDD61 pKa = 3.11SSSLNEE67 pKa = 3.85ASYY70 pKa = 9.08TAFNRR75 pKa = 11.84SLEE78 pKa = 4.25CVNASSEE85 pKa = 3.92DD86 pKa = 3.44WNAVFMVFFNEE97 pKa = 4.06PVNDD101 pKa = 3.8FEE103 pKa = 4.77LAYY106 pKa = 10.75SAGLKK111 pKa = 10.34ALEE114 pKa = 5.0ADD116 pKa = 4.34PEE118 pKa = 5.07DD119 pKa = 4.1DD120 pKa = 4.24VAWNYY125 pKa = 8.63FGCVIDD131 pKa = 5.71GLAHH135 pKa = 6.18NNSDD139 pKa = 3.81ADD141 pKa = 3.97LNEE144 pKa = 4.37PFDD147 pKa = 4.11AFKK150 pKa = 11.04KK151 pKa = 10.68AMSLNPTALYY161 pKa = 9.9SGNVAYY167 pKa = 10.38YY168 pKa = 10.69AIVTGNYY175 pKa = 9.43SYY177 pKa = 11.36AVEE180 pKa = 4.18ITDD183 pKa = 3.79QAVEE187 pKa = 4.34YY188 pKa = 9.69YY189 pKa = 10.86SPDD192 pKa = 3.12EE193 pKa = 4.1WSKK196 pKa = 10.28GTLFFLKK203 pKa = 10.46ALALAEE209 pKa = 4.31TGSSEE214 pKa = 3.95EE215 pKa = 3.72ALEE218 pKa = 4.37NIEE221 pKa = 5.0LSRR224 pKa = 11.84EE225 pKa = 4.13AYY227 pKa = 10.51NSDD230 pKa = 4.27EE231 pKa = 4.18SFEE234 pKa = 4.07WDD236 pKa = 3.42EE237 pKa = 4.0YY238 pKa = 11.28FYY240 pKa = 10.95ATDD243 pKa = 3.6GMVSAIALNDD253 pKa = 3.66LGRR256 pKa = 11.84SDD258 pKa = 4.03EE259 pKa = 4.25SAEE262 pKa = 3.51ILARR266 pKa = 11.84IDD268 pKa = 3.29TDD270 pKa = 3.84KK271 pKa = 11.44LDD273 pKa = 5.78DD274 pKa = 3.97YY275 pKa = 11.08MQGLLFTPEE284 pKa = 4.52RR285 pKa = 11.84YY286 pKa = 8.79MDD288 pKa = 4.42LYY290 pKa = 11.32GVALSGLGRR299 pKa = 11.84TDD301 pKa = 3.69DD302 pKa = 3.63AATAFEE308 pKa = 5.41KK309 pKa = 10.85SLEE312 pKa = 3.96YY313 pKa = 10.98NIDD316 pKa = 3.72YY317 pKa = 10.73IPAKK321 pKa = 10.48NHH323 pKa = 6.82LDD325 pKa = 3.49NLNQKK330 pKa = 9.5ISQQ333 pKa = 3.47

Molecular weight: 36.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H1YWU4|H1YWU4_9EURY 1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase OS=Methanoplanus limicola DSM 2279 OX=937775 GN=Metlim_2800 PE=4 SV=1
MM1 pKa = 7.47AAKK4 pKa = 9.99KK5 pKa = 10.2SSKK8 pKa = 9.27TAFKK12 pKa = 9.93KK13 pKa = 10.23IAMEE17 pKa = 5.05RR18 pKa = 11.84IDD20 pKa = 4.96ILFKK24 pKa = 10.08NAGRR28 pKa = 11.84EE29 pKa = 3.96FSNNPEE35 pKa = 3.76LSNRR39 pKa = 11.84YY40 pKa = 8.72VDD42 pKa = 3.23IARR45 pKa = 11.84RR46 pKa = 11.84IAMRR50 pKa = 11.84QRR52 pKa = 11.84VRR54 pKa = 11.84IPTEE58 pKa = 3.84YY59 pKa = 10.17KK60 pKa = 10.19RR61 pKa = 11.84KK62 pKa = 9.34FCRR65 pKa = 11.84NCHH68 pKa = 5.71SYY70 pKa = 10.87AVPGEE75 pKa = 4.05NMSVRR80 pKa = 11.84IHH82 pKa = 6.53RR83 pKa = 11.84GKK85 pKa = 10.71VIYY88 pKa = 10.15RR89 pKa = 11.84CSQCGYY95 pKa = 9.86IRR97 pKa = 11.84RR98 pKa = 11.84IPINNGDD105 pKa = 3.52RR106 pKa = 11.84KK107 pKa = 10.63

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2928

0

2928

906721

32

3686

309.7

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.868 ± 0.048

1.32 ± 0.022

6.119 ± 0.038

7.74 ± 0.056

4.17 ± 0.036

7.49 ± 0.044

1.609 ± 0.018

8.532 ± 0.054

6.311 ± 0.053

8.562 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.026

4.562 ± 0.051

3.948 ± 0.03

2.08 ± 0.022

4.711 ± 0.045

7.164 ± 0.05

5.106 ± 0.055

6.508 ± 0.042

0.906 ± 0.018

3.767 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski