Dickeya phage RC-2014

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Aglimvirinae; Limestonevirus; Dickeya virus RC2014

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075E121|A0A075E121_9CAUD DNA polymerase domain protein OS=Dickeya phage RC-2014 OX=1477406 GN=DA66_0143 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.23LNKK5 pKa = 9.49ILLVCALAFSTTACSTLLDD24 pKa = 3.75VASTVDD30 pKa = 3.92LDD32 pKa = 4.44APSFTNDD39 pKa = 2.92QAVSKK44 pKa = 10.76MEE46 pKa = 4.04DD47 pKa = 3.38TIKK50 pKa = 11.01AHH52 pKa = 6.89AALEE56 pKa = 4.16NSTPGPLQTVCNYY69 pKa = 10.58DD70 pKa = 3.97DD71 pKa = 5.54SIQEE75 pKa = 4.24DD76 pKa = 3.93EE77 pKa = 4.95TYY79 pKa = 10.8HH80 pKa = 5.42CTTYY84 pKa = 10.89VKK86 pKa = 10.16QASVVLYY93 pKa = 10.37ADD95 pKa = 3.83CTEE98 pKa = 4.36TQCVATGYY106 pKa = 11.21DD107 pKa = 3.23KK108 pKa = 11.7VEE110 pKa = 3.94EE111 pKa = 4.53DD112 pKa = 3.61KK113 pKa = 11.58

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075E0D8|A0A075E0D8_9CAUD PH domain protein OS=Dickeya phage RC-2014 OX=1477406 GN=DA66_0188 PE=4 SV=1
MM1 pKa = 7.53ANSIPILEE9 pKa = 4.55AVAKK13 pKa = 8.57EE14 pKa = 3.94NPRR17 pKa = 11.84ARR19 pKa = 11.84KK20 pKa = 8.14PRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84LTTKK28 pKa = 10.79LDD30 pKa = 3.08ILKK33 pKa = 10.26QKK35 pKa = 10.8FGGHH39 pKa = 5.04SVFTRR44 pKa = 11.84IRR46 pKa = 11.84NALKK50 pKa = 10.19EE51 pKa = 3.98GRR53 pKa = 11.84TEE55 pKa = 4.12LEE57 pKa = 3.91LYY59 pKa = 10.03RR60 pKa = 11.84PNGSTRR66 pKa = 11.84AYY68 pKa = 8.21QTTDD72 pKa = 2.69GLLEE76 pKa = 4.85LIRR79 pKa = 11.84LSGMTIEE86 pKa = 4.9PRR88 pKa = 11.84SSGTPLCSLYY98 pKa = 11.17VIGNLGALL106 pKa = 3.84

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

196

0

196

46405

42

1612

236.8

26.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.068 ± 0.194

1.11 ± 0.083

6.424 ± 0.122

6.588 ± 0.174

4.28 ± 0.106

6.719 ± 0.184

1.888 ± 0.103

5.958 ± 0.108

6.51 ± 0.193

8.049 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.926 ± 0.098

5.025 ± 0.1

4.017 ± 0.102

3.771 ± 0.126

4.993 ± 0.143

6.114 ± 0.156

6.126 ± 0.21

7.284 ± 0.15

1.42 ± 0.077

3.73 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski