Loktanella sp. S4079
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3296 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F4RL21|A0A0F4RL21_9RHOB Chemotaxis protein CheY OS=Loktanella sp. S4079 OX=579483 GN=TW80_11820 PE=4 SV=1
MM1 pKa = 7.33 LRR3 pKa = 11.84 WWVNADD9 pKa = 3.48 RR10 pKa = 11.84 KK11 pKa = 8.69 VTIVTITQKK20 pKa = 9.93 TIFPKK25 pKa = 10.61 RR26 pKa = 11.84 FDD28 pKa = 3.55 RR29 pKa = 11.84 KK30 pKa = 10.42 AIAVLAALSLSSALVTPDD48 pKa = 3.77 RR49 pKa = 11.84 LDD51 pKa = 4.05 AGDD54 pKa = 4.68 FTFDD58 pKa = 3.09 WSVIDD63 pKa = 3.73 WPEE66 pKa = 3.7 GSLGPNTYY74 pKa = 8.71 TLTDD78 pKa = 3.23 QYY80 pKa = 11.59 GFQIDD85 pKa = 3.74 SRR87 pKa = 11.84 IQMSGIFEE95 pKa = 4.65 SYY97 pKa = 11.04 GPANSPNDD105 pKa = 3.58 DD106 pKa = 3.85 VIFGGNVEE114 pKa = 4.22 SLILVSDD121 pKa = 4.21 APVGSANVGDD131 pKa = 4.08 ATVSTTLSFSSGGIVFPVDD150 pKa = 3.27 GLEE153 pKa = 4.83 LRR155 pKa = 11.84 VLDD158 pKa = 5.72 IDD160 pKa = 4.12 ATDD163 pKa = 3.84 NNNASDD169 pKa = 3.5 RR170 pKa = 11.84 CDD172 pKa = 3.32 FVTLTGNNGNPLLTVASATPTVLVGPGPGSGGTGALAANQAQCIYY217 pKa = 10.56 VDD219 pKa = 3.96 GFGASPTSNNDD230 pKa = 3.08 DD231 pKa = 3.64 TGTIIATYY239 pKa = 10.27 PNDD242 pKa = 3.77 TSSTTIFYY250 pKa = 10.66 DD251 pKa = 3.58 EE252 pKa = 4.96 SIGNVRR258 pKa = 11.84 PLFLGEE264 pKa = 4.32 DD265 pKa = 3.51 PAARR269 pKa = 11.84 GIGVLGDD276 pKa = 3.07 IAFNVDD282 pKa = 3.13 QSISLSRR289 pKa = 11.84 SVTPTTGLAGDD300 pKa = 4.07 SVTYY304 pKa = 10.12 EE305 pKa = 3.67 YY306 pKa = 11.49 VVTNNGALPFNTGQDD321 pKa = 4.05 LIIEE325 pKa = 4.81 DD326 pKa = 4.66 DD327 pKa = 4.13 LLGTVTCPAITAPVAPGGTVTCTAPYY353 pKa = 9.78 TITPADD359 pKa = 3.81 ALTGSVNSNATAGIGAIGQPFATRR383 pKa = 11.84 LQSNTANLNVVTITPPGEE401 pKa = 4.49 PGPQTCSPEE410 pKa = 4.35 SVFSKK415 pKa = 9.73 TRR417 pKa = 11.84 TQLAGPGSAAAPTTSDD433 pKa = 2.4 IFLYY437 pKa = 10.88 DD438 pKa = 4.3 DD439 pKa = 3.57 VTFDD443 pKa = 4.0 TNGNPIDD450 pKa = 4.32 IVMQLQEE457 pKa = 4.14 ISNAINVEE465 pKa = 3.81 ISSGLEE471 pKa = 3.58 ARR473 pKa = 11.84 MVPADD478 pKa = 3.85 NGYY481 pKa = 8.17 VTYY484 pKa = 10.67 SLRR487 pKa = 11.84 LVQDD491 pKa = 4.19 GSATPSNPQGTPIEE505 pKa = 4.05 LSRR508 pKa = 11.84 INGVIVQQTDD518 pKa = 2.64 VDD520 pKa = 4.03 SRR522 pKa = 11.84 GAGDD526 pKa = 4.18 DD527 pKa = 3.56 SSDD530 pKa = 3.68 VVGVSLTPTTLTHH543 pKa = 7.21 FNTAPLASFPAAGTAIAMDD562 pKa = 4.17 PAKK565 pKa = 10.34 TGDD568 pKa = 3.53 PTNWTDD574 pKa = 4.04 EE575 pKa = 4.37 PNEE578 pKa = 4.16 TAFDD582 pKa = 3.69 NYY584 pKa = 8.13 VTYY587 pKa = 10.61 EE588 pKa = 3.8 FDD590 pKa = 3.46 SFQEE594 pKa = 4.26 GTFIHH599 pKa = 7.43 GYY601 pKa = 7.93 TGTSINPATRR611 pKa = 11.84 GSGILLCAIANTSPTVVAEE630 pKa = 4.42 DD631 pKa = 4.31 DD632 pKa = 5.23 DD633 pKa = 4.53 YY634 pKa = 11.79 TATPINSVGGGTTGEE649 pKa = 4.08 VMANDD654 pKa = 5.05 TINGLPATFLNATISVISEE673 pKa = 4.0 ATPVTTGALVPTLVTSGVDD692 pKa = 3.12 EE693 pKa = 4.59 GRR695 pKa = 11.84 VVVPDD700 pKa = 3.92 GVPAGNYY707 pKa = 9.05 FIEE710 pKa = 4.42 YY711 pKa = 9.27 LLCDD715 pKa = 3.5 ATDD718 pKa = 3.46 TTDD721 pKa = 3.34 CDD723 pKa = 3.61 RR724 pKa = 11.84 AKK726 pKa = 10.14 VTISVYY732 pKa = 10.64 DD733 pKa = 3.51 GNGFDD738 pKa = 5.84 FGDD741 pKa = 4.35 APPSYY746 pKa = 9.23 LTASHH751 pKa = 6.73 GVPDD755 pKa = 3.98 TPVIYY760 pKa = 10.48 LGVVPPDD767 pKa = 3.61 AEE769 pKa = 4.77 LVAQSDD775 pKa = 3.93 ATATADD781 pKa = 4.17 DD782 pKa = 4.4 VFNIDD787 pKa = 5.34 DD788 pKa = 3.98 EE789 pKa = 4.91 DD790 pKa = 4.0 AVSFPILTQGTISTLDD806 pKa = 3.17 VGVTGNGTLQAWIDD820 pKa = 3.87 FNGDD824 pKa = 3.3 GLFEE828 pKa = 4.05 QTLGEE833 pKa = 4.8 RR834 pKa = 11.84 IATDD838 pKa = 3.51 LRR840 pKa = 11.84 DD841 pKa = 4.26 DD842 pKa = 4.36 GSAFDD847 pKa = 4.63 NVAGDD852 pKa = 3.85 GVIQIDD858 pKa = 3.87 VAVPTDD864 pKa = 3.4 ATTDD868 pKa = 3.2 TTFARR873 pKa = 11.84 FRR875 pKa = 11.84 YY876 pKa = 7.75 STSPGLSPTDD886 pKa = 3.77 FAPDD890 pKa = 3.92 GEE892 pKa = 4.53 VEE894 pKa = 4.61 DD895 pKa = 4.39 YY896 pKa = 11.45 SLVIAAADD904 pKa = 3.6 LVDD907 pKa = 5.34 RR908 pKa = 11.84 GDD910 pKa = 3.89 APASYY915 pKa = 10.6 GDD917 pKa = 3.68 PRR919 pKa = 11.84 HH920 pKa = 6.61 IIVDD924 pKa = 4.47 DD925 pKa = 3.73 IFLGAGIPDD934 pKa = 4.54 SEE936 pKa = 4.42 TDD938 pKa = 3.5 PQNSVDD944 pKa = 4.55 ADD946 pKa = 3.73 ADD948 pKa = 3.99 DD949 pKa = 4.19 FAGADD954 pKa = 4.15 DD955 pKa = 4.43 EE956 pKa = 5.92 DD957 pKa = 4.53 SVAVFPVFEE966 pKa = 4.35 VGTTVSVTVQTHH978 pKa = 4.6 EE979 pKa = 4.31 TLSLQYY985 pKa = 11.49 ALGIPVTSGVTNLQAWIDD1003 pKa = 3.7 FDD1005 pKa = 4.34 QNGTFEE1011 pKa = 4.48 PSEE1014 pKa = 4.02 QVATNYY1020 pKa = 10.17 RR1021 pKa = 11.84 DD1022 pKa = 3.49 GGIGDD1027 pKa = 3.69 TDD1029 pKa = 4.73 GIFNNQISFDD1039 pKa = 3.68 VTVPNTISSGYY1050 pKa = 8.49 TYY1052 pKa = 11.42 ARR1054 pKa = 11.84 LRR1056 pKa = 11.84 WSTSSALVSDD1066 pKa = 4.82 PFDD1069 pKa = 3.53 GLNFDD1074 pKa = 5.32 GEE1076 pKa = 4.54 VEE1078 pKa = 4.22 DD1079 pKa = 4.61 YY1080 pKa = 11.11 EE1081 pKa = 4.75 VVLSAGAVPFEE1092 pKa = 4.74 CDD1094 pKa = 2.74 GTLYY1098 pKa = 10.33 RR1099 pKa = 11.84 VARR1102 pKa = 11.84 VDD1104 pKa = 3.36 SQLQRR1109 pKa = 11.84 LVFSEE1114 pKa = 4.78 DD1115 pKa = 2.93 GSGGYY1120 pKa = 8.7 TIDD1123 pKa = 3.62 ATNVGAPAGVAYY1135 pKa = 10.79 NGGWGYY1141 pKa = 10.77 NAVDD1145 pKa = 3.37 GLFYY1149 pKa = 10.69 GVRR1152 pKa = 11.84 EE1153 pKa = 4.1 FEE1155 pKa = 4.22 RR1156 pKa = 11.84 DD1157 pKa = 3.38 LVQLDD1162 pKa = 3.61 STGKK1166 pKa = 8.01 FTVVSTLPPTAATGYY1181 pKa = 10.28 NSGDD1185 pKa = 3.33 ILSNGIMIYY1194 pKa = 10.11 RR1195 pKa = 11.84 VQGTNDD1201 pKa = 3.36 FQLLDD1206 pKa = 3.57 ITDD1209 pKa = 3.77 PTNPIDD1215 pKa = 4.21 AGLVTLTSAVDD1226 pKa = 3.57 PFDD1229 pKa = 4.03 IAFNPNDD1236 pKa = 3.21 GMIYY1240 pKa = 10.21 GVNHH1244 pKa = 4.57 VTDD1247 pKa = 3.56 RR1248 pKa = 11.84 LFYY1251 pKa = 10.55 FDD1253 pKa = 5.09 PADD1256 pKa = 3.98 GAPGTRR1262 pKa = 11.84 TPVEE1266 pKa = 4.27 FGPAIWTEE1274 pKa = 3.9 AYY1276 pKa = 9.86 GAIWFDD1282 pKa = 3.72 FYY1284 pKa = 11.75 GRR1286 pKa = 11.84 MYY1288 pKa = 11.16 VNQNTSNEE1296 pKa = 4.14 MYY1298 pKa = 9.96 EE1299 pKa = 4.04 VDD1301 pKa = 5.34 VGIQGNGTGNRR1312 pKa = 11.84 EE1313 pKa = 4.64 LISVITISEE1322 pKa = 4.07 EE1323 pKa = 3.7 FRR1325 pKa = 11.84 NDD1327 pKa = 3.26 GAGCPSRR1334 pKa = 11.84 LGPLPPEE1341 pKa = 4.25 GALAGTVYY1349 pKa = 10.87 EE1350 pKa = 4.56 DD1351 pKa = 3.9 TDD1353 pKa = 3.91 GSGAFDD1359 pKa = 3.57 NGEE1362 pKa = 3.98 TGIPNISVDD1371 pKa = 3.23 IYY1373 pKa = 11.2 NDD1375 pKa = 2.99 NGTPGTIVDD1384 pKa = 4.32 DD1385 pKa = 4.08 VLVTSVVTAADD1396 pKa = 3.52 GSYY1399 pKa = 10.62 LVEE1402 pKa = 4.65 NLSALNTYY1410 pKa = 10.23 RR1411 pKa = 11.84 IEE1413 pKa = 4.6 VEE1415 pKa = 4.61 DD1416 pKa = 4.96 ADD1418 pKa = 4.41 ADD1420 pKa = 3.99 MPAGYY1425 pKa = 10.18 QSVTPNPLTAIRR1437 pKa = 11.84 VTAGSTRR1444 pKa = 11.84 GGLDD1448 pKa = 3.13 FGFAPGEE1455 pKa = 4.01 ADD1457 pKa = 3.97 LSITKK1462 pKa = 8.87 TVEE1465 pKa = 3.87 RR1466 pKa = 11.84 TSDD1469 pKa = 3.29 GATVTSAAEE1478 pKa = 4.1 GTEE1481 pKa = 4.07 LEE1483 pKa = 4.41 FVISVTNSGPASVASVLVNDD1503 pKa = 4.68 ILPSGFAYY1511 pKa = 10.76 VSDD1514 pKa = 4.36 DD1515 pKa = 3.92 AASQGNTYY1523 pKa = 10.3 NASTGRR1529 pKa = 11.84 WDD1531 pKa = 3.56 VGGLAIGEE1539 pKa = 4.47 TKK1541 pKa = 9.38 TLRR1544 pKa = 11.84 ITVTMLGSGNHH1555 pKa = 4.91 TNIAEE1560 pKa = 4.48 IIFSSVDD1567 pKa = 3.83 DD1568 pKa = 4.76 PDD1570 pKa = 4.33 SDD1572 pKa = 4.14 PSVGTATDD1580 pKa = 4.13 DD1581 pKa = 4.14 LSDD1584 pKa = 5.47 GIPDD1588 pKa = 4.14 DD1589 pKa = 5.58 DD1590 pKa = 4.07 EE1591 pKa = 7.09 ASVTVALDD1599 pKa = 3.16 NGEE1602 pKa = 4.22 RR1603 pKa = 11.84 LLSGRR1608 pKa = 11.84 VFIDD1612 pKa = 2.9 NGVNSGTAHH1621 pKa = 6.74 DD1622 pKa = 5.25 AIVNGSEE1629 pKa = 4.19 TGTQSATLTILDD1641 pKa = 3.89 GSGSVIATPTIAADD1655 pKa = 4.13 GSWAYY1660 pKa = 10.58 ALDD1663 pKa = 3.98 GSYY1666 pKa = 10.49 SGPLSVSVAPLPGFITVSEE1685 pKa = 4.23 NDD1687 pKa = 3.22 SGLPALANSDD1697 pKa = 3.45 PHH1699 pKa = 7.71 DD1700 pKa = 4.32 GNFTFTPDD1708 pKa = 2.93 ASSNYY1713 pKa = 9.45 SGLNIGIVRR1722 pKa = 11.84 APTLTRR1728 pKa = 11.84 DD1729 pKa = 3.32 QEE1731 pKa = 4.22 ASVEE1735 pKa = 4.25 SGQVATLQHH1744 pKa = 7.28 EE1745 pKa = 4.69 YY1746 pKa = 9.84 TAFTSGTVTFSYY1758 pKa = 10.82 ANPNMAPPNSFSATLYY1774 pKa = 10.72 RR1775 pKa = 11.84 DD1776 pKa = 3.75 TDD1778 pKa = 3.92 CDD1780 pKa = 3.91 TTPDD1784 pKa = 3.66 TPINAPITVSAGDD1797 pKa = 3.8 RR1798 pKa = 11.84 LCLVSRR1804 pKa = 11.84 VTANSGIGPGSSYY1817 pKa = 10.78 TYY1819 pKa = 11.15 DD1820 pKa = 3.52 LVASTALSATTVVSTSQNTDD1840 pKa = 3.46 RR1841 pKa = 11.84 IVAGGGAGRR1850 pKa = 11.84 LEE1852 pKa = 3.87 LRR1854 pKa = 11.84 KK1855 pKa = 7.74 TVRR1858 pKa = 11.84 NITQNTPEE1866 pKa = 4.26 GASNGGAIGDD1876 pKa = 3.65 VLRR1879 pKa = 11.84 YY1880 pKa = 9.56 QIYY1883 pKa = 10.51 LRR1885 pKa = 11.84 NPSSAPATNVIIYY1898 pKa = 9.45 DD1899 pKa = 3.59 KK1900 pKa = 9.93 TPSYY1904 pKa = 9.52 TALASPIASPTTLGNNLICVVSQPTNNVAGYY1935 pKa = 9.46 VGPLRR1940 pKa = 11.84 WDD1942 pKa = 3.32 CTGTYY1947 pKa = 10.35 EE1948 pKa = 4.92 PGEE1951 pKa = 4.45 DD1952 pKa = 3.26 DD1953 pKa = 4.73 SVFFDD1958 pKa = 4.36 VVISPP1963 pKa = 4.35
Molecular weight: 204.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.683
IPC2_protein 3.617
IPC_protein 3.681
Toseland 3.439
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.338
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.088
Thurlkill 3.49
EMBOSS 3.643
Sillero 3.808
Patrickios 1.392
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.723
Protein with the highest isoelectric point:
>tr|A0A0F4RL47|A0A0F4RL47_9RHOB Uncharacterized protein OS=Loktanella sp. S4079 OX=579483 GN=TW80_05690 PE=4 SV=1
MM1 pKa = 6.48 TQRR4 pKa = 11.84 VDD6 pKa = 3.31 VAANRR11 pKa = 11.84 AARR14 pKa = 11.84 KK15 pKa = 9.54 YY16 pKa = 10.61 DD17 pKa = 3.11 ALTQVKK23 pKa = 9.31 RR24 pKa = 11.84 VLWALCWPLFRR35 pKa = 11.84 LSPRR39 pKa = 11.84 PMWGWRR45 pKa = 11.84 VWILRR50 pKa = 11.84 AFGAQVGRR58 pKa = 11.84 GVHH61 pKa = 6.0 VYY63 pKa = 9.09 PSARR67 pKa = 11.84 ITMPWHH73 pKa = 6.31 LRR75 pKa = 11.84 LADD78 pKa = 3.7 EE79 pKa = 4.38 VAIGDD84 pKa = 3.93 RR85 pKa = 11.84 AILYY89 pKa = 9.96 ALDD92 pKa = 4.22 VITIGARR99 pKa = 11.84 ATVSQYY105 pKa = 11.68 AHH107 pKa = 7.41 LCTGSHH113 pKa = 7.3 DD114 pKa = 4.21 LGDD117 pKa = 3.68 PARR120 pKa = 11.84 ALKK123 pKa = 8.46 TAPIMIGADD132 pKa = 2.78 AWVCADD138 pKa = 3.66 AFVGPGVQIGKK149 pKa = 9.28 SAVLGARR156 pKa = 11.84 AVAFKK161 pKa = 10.58 ALPDD165 pKa = 3.46 RR166 pKa = 11.84 CVGIGNPMQIRR177 pKa = 11.84 SRR179 pKa = 11.84 HH180 pKa = 5.08 DD181 pKa = 2.95
Molecular weight: 19.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.502
IPC_protein 10.292
Toseland 10.35
ProMoST 10.116
Dawson 10.526
Bjellqvist 10.277
Wikipedia 10.745
Rodwell 10.657
Grimsley 10.584
Solomon 10.613
Lehninger 10.57
Nozaki 10.409
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.452
Patrickios 10.35
IPC_peptide 10.613
IPC2_peptide 9.545
IPC2.peptide.svr19 8.459
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3296
0
3296
1023294
33
1963
310.5
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.625 ± 0.051
0.911 ± 0.013
6.269 ± 0.037
5.726 ± 0.042
3.859 ± 0.031
8.338 ± 0.047
2.074 ± 0.022
5.788 ± 0.031
3.447 ± 0.038
9.6 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.827 ± 0.02
3.067 ± 0.021
4.765 ± 0.028
3.558 ± 0.03
5.943 ± 0.041
5.376 ± 0.027
5.77 ± 0.032
7.33 ± 0.039
1.365 ± 0.018
2.363 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here