Ceratitis capitata sigmavirus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140D8P3|A0A140D8P3_9RHAB Polymerase-associated protein OS=Ceratitis capitata sigmavirus OX=1802949 GN=P PE=4 SV=1
MM1 pKa = 6.33 TTNGKK6 pKa = 10.18 KK7 pKa = 9.62 IVNINSKK14 pKa = 10.01 KK15 pKa = 10.36 SYY17 pKa = 10.29 VVPCVYY23 pKa = 10.72 AEE25 pKa = 4.31 RR26 pKa = 11.84 SPEE29 pKa = 4.25 FPIDD33 pKa = 3.25 WFDD36 pKa = 3.44 TTRR39 pKa = 11.84 DD40 pKa = 3.63 KK41 pKa = 11.14 PILNIQIFKK50 pKa = 10.83 EE51 pKa = 3.95 CDD53 pKa = 2.99 LDD55 pKa = 4.24 KK56 pKa = 11.26 ARR58 pKa = 11.84 QYY60 pKa = 12.09 ADD62 pKa = 3.7 AFLKK66 pKa = 9.57 GTIHH70 pKa = 5.64 GTIPVTTYY78 pKa = 10.9 LYY80 pKa = 11.22 YY81 pKa = 11.2 LFLTAKK87 pKa = 7.73 EE88 pKa = 4.16 TLTRR92 pKa = 11.84 DD93 pKa = 2.87 WTSYY97 pKa = 10.35 RR98 pKa = 11.84 MNLKK102 pKa = 10.31 ASEE105 pKa = 4.18 QVTPLSLLTVNKK117 pKa = 10.3 EE118 pKa = 4.3 EE119 pKa = 4.31 VDD121 pKa = 3.54 QTPLTNPTTLDD132 pKa = 3.51 NNSDD136 pKa = 3.5 KK137 pKa = 11.18 SILLALVGIYY147 pKa = 9.92 RR148 pKa = 11.84 LHH150 pKa = 5.45 TTHH153 pKa = 7.45 PALVDD158 pKa = 3.32 IVTDD162 pKa = 4.94 RR163 pKa = 11.84 INLLIQQATPSDD175 pKa = 3.53 KK176 pKa = 10.74 VQYY179 pKa = 10.84 SVDD182 pKa = 3.66 LAKK185 pKa = 10.34 TNSGYY190 pKa = 11.09 LSGNDD195 pKa = 3.21 SVEE198 pKa = 4.13 ILLSALDD205 pKa = 3.64 MFADD209 pKa = 4.18 KK210 pKa = 10.85 FPANKK215 pKa = 9.85 YY216 pKa = 7.67 SQARR220 pKa = 11.84 IGTIILRR227 pKa = 11.84 YY228 pKa = 9.21 AGCSALLDD236 pKa = 3.8 LTYY239 pKa = 7.27 MTKK242 pKa = 9.92 MIACDD247 pKa = 3.46 GVLDD251 pKa = 3.74 VLQWVFLPRR260 pKa = 11.84 VGQEE264 pKa = 3.64 LDD266 pKa = 3.24 AMLSKK271 pKa = 10.67 EE272 pKa = 3.92 DD273 pKa = 4.04 SEE275 pKa = 4.36 ITKK278 pKa = 10.07 EE279 pKa = 4.1 DD280 pKa = 3.75 SYY282 pKa = 11.77 FPYY285 pKa = 11.05 LLGLRR290 pKa = 11.84 LSSKK294 pKa = 10.5 SPYY297 pKa = 9.26 AASSAPQLHH306 pKa = 6.5 HH307 pKa = 6.87 LVHH310 pKa = 6.7 AVGSLMGLSRR320 pKa = 11.84 SINALLIDD328 pKa = 4.46 PGTPNMVANNAALIFLANKK347 pKa = 9.5 RR348 pKa = 11.84 LSGLKK353 pKa = 9.29 VVYY356 pKa = 10.05 MNEE359 pKa = 3.8 DD360 pKa = 3.54 DD361 pKa = 5.14 AKK363 pKa = 11.26 VNQTQQEE370 pKa = 4.48 KK371 pKa = 10.68 ASQTRR376 pKa = 11.84 QQNISEE382 pKa = 4.09 EE383 pKa = 4.4 DD384 pKa = 3.46 ALSSRR389 pKa = 11.84 DD390 pKa = 4.4 LDD392 pKa = 4.19 DD393 pKa = 4.84 EE394 pKa = 4.44 QPKK397 pKa = 9.0 TPRR400 pKa = 11.84 DD401 pKa = 3.3 WFNWYY406 pKa = 9.61 CDD408 pKa = 4.05 KK409 pKa = 11.04 DD410 pKa = 3.45 WKK412 pKa = 9.21 FTKK415 pKa = 10.14 KK416 pKa = 10.15 EE417 pKa = 3.76 YY418 pKa = 11.11 LEE420 pKa = 4.2 IRR422 pKa = 11.84 DD423 pKa = 3.54 AVMSIKK429 pKa = 10.4 NPRR432 pKa = 11.84 SGTVGAWTVEE442 pKa = 4.27 TFLSLINADD451 pKa = 3.46 IYY453 pKa = 11.7
Molecular weight: 51.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.303
IPC2_protein 5.385
IPC_protein 5.359
Toseland 5.436
ProMoST 5.575
Dawson 5.448
Bjellqvist 5.537
Wikipedia 5.372
Rodwell 5.359
Grimsley 5.41
Solomon 5.448
Lehninger 5.41
Nozaki 5.601
DTASelect 5.817
Thurlkill 5.512
EMBOSS 5.512
Sillero 5.677
Patrickios 4.075
IPC_peptide 5.448
IPC2_peptide 5.664
IPC2.peptide.svr19 5.7
Protein with the highest isoelectric point:
>tr|A0A140D8P6|A0A140D8P6_9RHAB Glycoprotein OS=Ceratitis capitata sigmavirus OX=1802949 GN=G PE=4 SV=1
MM1 pKa = 7.39 NGKK4 pKa = 8.81 KK5 pKa = 9.91 QLVRR9 pKa = 11.84 FKK11 pKa = 10.16 EE12 pKa = 4.08 QTPTTSKK19 pKa = 10.88 NLSKK23 pKa = 10.45 PKK25 pKa = 10.47 EE26 pKa = 4.26 EE27 pKa = 3.89 ISSPFKK33 pKa = 10.52 PIYY36 pKa = 7.59 TTTLPSAPTLTPKK49 pKa = 9.91 VIKK52 pKa = 9.96 WDD54 pKa = 3.6 VTSSLRR60 pKa = 11.84 LTTNRR65 pKa = 11.84 RR66 pKa = 11.84 PKK68 pKa = 8.78 EE69 pKa = 3.96 WKK71 pKa = 10.1 SIQCILAVLIDD82 pKa = 4.62 EE83 pKa = 4.6 YY84 pKa = 11.17 AGPEE88 pKa = 3.53 MWRR91 pKa = 11.84 SVFEE95 pKa = 3.88 LFYY98 pKa = 10.99 WMLSRR103 pKa = 11.84 NIAEE107 pKa = 4.9 LPSSSDD113 pKa = 3.13 RR114 pKa = 11.84 YY115 pKa = 11.02 VYY117 pKa = 10.7 GADD120 pKa = 3.61 FNEE123 pKa = 4.36 LTLVHH128 pKa = 6.6 HH129 pKa = 6.95 NIYY132 pKa = 10.84 YY133 pKa = 10.78 LIDD136 pKa = 3.35 QGQDD140 pKa = 3.13 YY141 pKa = 10.09 HH142 pKa = 6.44 FHH144 pKa = 5.34 YY145 pKa = 9.49 TGKK148 pKa = 9.79 IRR150 pKa = 11.84 DD151 pKa = 3.76 MKK153 pKa = 10.2 YY154 pKa = 10.58 SIVFSCSLKK163 pKa = 8.58 PTKK166 pKa = 9.98 RR167 pKa = 11.84 DD168 pKa = 3.41 CYY170 pKa = 10.83 SIARR174 pKa = 11.84 LQEE177 pKa = 4.01 YY178 pKa = 10.45 KK179 pKa = 10.84 SIIYY183 pKa = 10.24 EE184 pKa = 4.71 DD185 pKa = 4.0 IPDD188 pKa = 3.93 LTTVLTLGQVKK199 pKa = 10.42 YY200 pKa = 10.78 MMLNQTIVLHH210 pKa = 5.35 MM211 pKa = 4.68
Molecular weight: 24.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.492
IPC2_protein 8.478
IPC_protein 8.404
Toseland 8.843
ProMoST 8.799
Dawson 9.209
Bjellqvist 9.077
Wikipedia 9.385
Rodwell 9.326
Grimsley 9.253
Solomon 9.297
Lehninger 9.253
Nozaki 9.092
DTASelect 9.004
Thurlkill 9.063
EMBOSS 9.326
Sillero 9.238
Patrickios 4.202
IPC_peptide 9.282
IPC2_peptide 7.805
IPC2.peptide.svr19 7.692
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3981
211
2152
663.5
76.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.391 ± 0.596
1.658 ± 0.151
5.526 ± 0.587
4.999 ± 0.223
4.295 ± 0.421
4.446 ± 0.539
2.914 ± 0.28
8.415 ± 0.481
6.33 ± 0.23
10.952 ± 0.249
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.035 ± 0.226
6.556 ± 0.35
4.496 ± 0.308
3.542 ± 0.273
4.521 ± 0.192
8.314 ± 0.274
6.883 ± 0.279
4.974 ± 0.402
1.557 ± 0.267
4.195 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here