Asian prunus virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3DVM2|A0A0U3DVM2_9VIRU Movement protein TGB2 OS=Asian prunus virus 2 OX=351426 PE=3 SV=1
MM1 pKa = 7.84DD2 pKa = 4.44FVYY5 pKa = 10.81DD6 pKa = 3.72KK7 pKa = 11.13LIDD10 pKa = 3.62AGYY13 pKa = 10.13IRR15 pKa = 11.84TRR17 pKa = 11.84LPISFPIIVHH27 pKa = 5.93CVAGAGKK34 pKa = 8.42STLIRR39 pKa = 11.84EE40 pKa = 4.55IIEE43 pKa = 3.86VDD45 pKa = 3.33HH46 pKa = 6.84KK47 pKa = 11.46FEE49 pKa = 4.59AFTYY53 pKa = 9.3GVPDD57 pKa = 5.46PINLSGVRR65 pKa = 11.84IKK67 pKa = 11.05SNSDD71 pKa = 2.88IPSARR76 pKa = 11.84ADD78 pKa = 3.38SLKK81 pKa = 10.72IIDD84 pKa = 4.5EE85 pKa = 4.67YY86 pKa = 10.82IGQDD90 pKa = 3.55LPEE93 pKa = 4.32GTTFCFADD101 pKa = 3.95PNQFPYY107 pKa = 9.88TCPDD111 pKa = 2.85AHH113 pKa = 5.76FTCYY117 pKa = 6.35QTKK120 pKa = 10.17RR121 pKa = 11.84FGDD124 pKa = 3.56QTCALLGKK132 pKa = 9.4IDD134 pKa = 4.21CAAFSYY140 pKa = 10.61KK141 pKa = 9.61QDD143 pKa = 3.39QIIFDD148 pKa = 4.15KK149 pKa = 11.18LFAGDD154 pKa = 3.57IEE156 pKa = 4.46GQIVCYY162 pKa = 9.81EE163 pKa = 4.04KK164 pKa = 10.88EE165 pKa = 4.03IFDD168 pKa = 4.38LLDD171 pKa = 3.15RR172 pKa = 11.84HH173 pKa = 5.81GADD176 pKa = 3.17YY177 pKa = 11.03KK178 pKa = 10.82KK179 pKa = 9.7YY180 pKa = 8.56CQIRR184 pKa = 11.84GSTFDD189 pKa = 2.89IVTFITASDD198 pKa = 3.53TFEE201 pKa = 5.1PEE203 pKa = 3.57DD204 pKa = 3.71RR205 pKa = 11.84YY206 pKa = 10.97KK207 pKa = 11.12VYY209 pKa = 11.09LCLTRR214 pKa = 11.84HH215 pKa = 5.76RR216 pKa = 11.84SILRR220 pKa = 11.84ILSPEE225 pKa = 4.06GKK227 pKa = 9.86FLRR230 pKa = 11.84EE231 pKa = 3.72DD232 pKa = 3.35AKK234 pKa = 11.16FDD236 pKa = 3.53TTTT239 pKa = 3.14

Molecular weight: 27.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2I7H9|Q2I7H9_9VIRU Movement protein TGBp3 OS=Asian prunus virus 2 OX=351426 GN=TGBp3 PE=3 SV=1
MM1 pKa = 7.86PSLTPPPDD9 pKa = 3.3NTRR12 pKa = 11.84VLLPIAIGLGVGVVIWCLTRR32 pKa = 11.84STLPQVGDD40 pKa = 4.14NIHH43 pKa = 6.29SLPHH47 pKa = 6.02GGNYY51 pKa = 10.08LDD53 pKa = 3.48GTKK56 pKa = 10.09RR57 pKa = 11.84ISYY60 pKa = 9.1CGPKK64 pKa = 10.3NSFPSSNLFKK74 pKa = 11.01GGAFSAICVVVLLVFAIHH92 pKa = 6.53VSEE95 pKa = 4.86LFNRR99 pKa = 11.84PNRR102 pKa = 11.84RR103 pKa = 11.84SCGCGSASHH112 pKa = 6.65AA113 pKa = 4.27

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2822

65

2005

564.4

63.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.989 ± 0.734

2.303 ± 0.556

4.961 ± 0.907

6.343 ± 0.756

6.237 ± 0.775

6.201 ± 0.453

2.516 ± 0.332

5.386 ± 1.327

6.308 ± 0.408

9.639 ± 1.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.343

4.89 ± 0.898

4.5 ± 0.694

2.941 ± 0.191

5.245 ± 0.288

9.107 ± 1.032

4.996 ± 1.082

6.875 ± 0.592

0.85 ± 0.162

2.87 ± 0.472
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski