Amino acid dipepetide frequency for Prochlorococcus phage P-TIM68

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.386AlaAla: 4.386 ± 0.308
0.686AlaCys: 0.686 ± 0.119
3.126AlaAsp: 3.126 ± 0.212
3.381AlaGlu: 3.381 ± 0.247
2.536AlaPhe: 2.536 ± 0.225
5.566AlaGly: 5.566 ± 0.41
1.18AlaHis: 1.18 ± 0.176
3.923AlaIle: 3.923 ± 0.3
3.78AlaLys: 3.78 ± 0.431
4.386AlaLeu: 4.386 ± 0.28
1.388AlaMet: 1.388 ± 0.158
3.764AlaAsn: 3.764 ± 0.275
2.297AlaPro: 2.297 ± 0.235
2.376AlaGln: 2.376 ± 0.21
2.488AlaArg: 2.488 ± 0.236
4.864AlaSer: 4.864 ± 0.298
4.721AlaThr: 4.721 ± 0.415
3.907AlaVal: 3.907 ± 0.31
0.574AlaTrp: 0.574 ± 0.124
1.946AlaTyr: 1.946 ± 0.182
0.0AlaXaa: 0.0 ± 0.0
Cys
0.718CysAla: 0.718 ± 0.111
0.08CysCys: 0.08 ± 0.037
0.638CysAsp: 0.638 ± 0.114
0.766CysGlu: 0.766 ± 0.136
0.415CysPhe: 0.415 ± 0.09
0.558CysGly: 0.558 ± 0.111
0.191CysHis: 0.191 ± 0.065
0.67CysIle: 0.67 ± 0.11
0.59CysLys: 0.59 ± 0.091
0.67CysLeu: 0.67 ± 0.116
0.335CysMet: 0.335 ± 0.084
0.319CysAsn: 0.319 ± 0.075
0.335CysPro: 0.335 ± 0.103
0.287CysGln: 0.287 ± 0.067
0.558CysArg: 0.558 ± 0.098
0.526CysSer: 0.526 ± 0.097
0.542CysThr: 0.542 ± 0.084
0.718CysVal: 0.718 ± 0.083
0.096CysTrp: 0.096 ± 0.039
0.335CysTyr: 0.335 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
3.732AspAla: 3.732 ± 0.225
0.59AspCys: 0.59 ± 0.105
4.322AspAsp: 4.322 ± 0.333
3.955AspGlu: 3.955 ± 0.291
3.381AspPhe: 3.381 ± 0.249
5.47AspGly: 5.47 ± 0.328
1.069AspHis: 1.069 ± 0.169
4.785AspIle: 4.785 ± 0.296
4.099AspLys: 4.099 ± 0.373
5.614AspLeu: 5.614 ± 0.302
1.292AspMet: 1.292 ± 0.171
3.557AspAsn: 3.557 ± 0.235
2.52AspPro: 2.52 ± 0.191
1.77AspGln: 1.77 ± 0.177
2.887AspArg: 2.887 ± 0.238
4.067AspSer: 4.067 ± 0.244
3.812AspThr: 3.812 ± 0.267
4.498AspVal: 4.498 ± 0.267
0.973AspTrp: 0.973 ± 0.157
3.27AspTyr: 3.27 ± 0.274
0.0AspXaa: 0.0 ± 0.0
Glu
3.27GluAla: 3.27 ± 0.254
0.606GluCys: 0.606 ± 0.114
4.051GluAsp: 4.051 ± 0.344
5.008GluGlu: 5.008 ± 0.49
2.791GluPhe: 2.791 ± 0.231
3.429GluGly: 3.429 ± 0.225
0.925GluHis: 0.925 ± 0.127
4.641GluIle: 4.641 ± 0.368
4.753GluLys: 4.753 ± 0.517
4.833GluLeu: 4.833 ± 0.372
1.435GluMet: 1.435 ± 0.185
3.62GluAsn: 3.62 ± 0.274
1.93GluPro: 1.93 ± 0.23
2.121GluGln: 2.121 ± 0.246
2.632GluArg: 2.632 ± 0.23
3.62GluSer: 3.62 ± 0.228
3.557GluThr: 3.557 ± 0.233
4.498GluVal: 4.498 ± 0.304
0.718GluTrp: 0.718 ± 0.128
2.504GluTyr: 2.504 ± 0.248
0.0GluXaa: 0.0 ± 0.0
Phe
2.775PheAla: 2.775 ± 0.197
0.542PheCys: 0.542 ± 0.095
3.955PheAsp: 3.955 ± 0.272
2.775PheGlu: 2.775 ± 0.206
2.026PhePhe: 2.026 ± 0.184
2.998PheGly: 2.998 ± 0.255
0.797PheHis: 0.797 ± 0.126
3.062PheIle: 3.062 ± 0.201
3.11PheLys: 3.11 ± 0.257
3.684PheLeu: 3.684 ± 0.302
0.973PheMet: 0.973 ± 0.125
2.903PheAsn: 2.903 ± 0.3
1.643PhePro: 1.643 ± 0.153
1.595PheGln: 1.595 ± 0.177
1.531PheArg: 1.531 ± 0.158
3.301PheSer: 3.301 ± 0.229
3.381PheThr: 3.381 ± 0.284
3.11PheVal: 3.11 ± 0.212
0.383PheTrp: 0.383 ± 0.08
1.738PheTyr: 1.738 ± 0.161
0.0PheXaa: 0.0 ± 0.0
Gly
5.407GlyAla: 5.407 ± 0.419
0.702GlyCys: 0.702 ± 0.115
4.545GlyAsp: 4.545 ± 0.331
3.477GlyGlu: 3.477 ± 0.263
3.541GlyPhe: 3.541 ± 0.27
7.097GlyGly: 7.097 ± 0.596
1.404GlyHis: 1.404 ± 0.166
6.172GlyIle: 6.172 ± 0.491
4.29GlyLys: 4.29 ± 0.405
5.455GlyLeu: 5.455 ± 0.331
1.499GlyMet: 1.499 ± 0.169
4.737GlyAsn: 4.737 ± 0.426
1.659GlyPro: 1.659 ± 0.152
2.887GlyGln: 2.887 ± 0.225
3.062GlyArg: 3.062 ± 0.233
6.427GlySer: 6.427 ± 0.524
6.3GlyThr: 6.3 ± 0.553
5.885GlyVal: 5.885 ± 0.453
0.989GlyTrp: 0.989 ± 0.145
2.935GlyTyr: 2.935 ± 0.226
0.0GlyXaa: 0.0 ± 0.0
His
0.845HisAla: 0.845 ± 0.122
0.271HisCys: 0.271 ± 0.072
1.085HisAsp: 1.085 ± 0.155
0.877HisGlu: 0.877 ± 0.142
0.973HisPhe: 0.973 ± 0.147
1.069HisGly: 1.069 ± 0.131
0.781HisHis: 0.781 ± 0.162
1.26HisIle: 1.26 ± 0.131
1.148HisLys: 1.148 ± 0.15
1.515HisLeu: 1.515 ± 0.204
0.51HisMet: 0.51 ± 0.086
0.989HisAsn: 0.989 ± 0.121
0.829HisPro: 0.829 ± 0.107
0.574HisGln: 0.574 ± 0.087
0.526HisArg: 0.526 ± 0.101
1.292HisSer: 1.292 ± 0.164
1.164HisThr: 1.164 ± 0.164
1.021HisVal: 1.021 ± 0.13
0.287HisTrp: 0.287 ± 0.074
0.766HisTyr: 0.766 ± 0.122
0.0HisXaa: 0.0 ± 0.0
Ile
4.115IleAla: 4.115 ± 0.307
0.67IleCys: 0.67 ± 0.107
4.801IleAsp: 4.801 ± 0.309
4.721IleGlu: 4.721 ± 0.327
2.695IlePhe: 2.695 ± 0.194
5.215IleGly: 5.215 ± 0.328
1.324IleHis: 1.324 ± 0.151
4.514IleIle: 4.514 ± 0.294
4.737IleLys: 4.737 ± 0.31
4.657IleLeu: 4.657 ± 0.28
1.244IleMet: 1.244 ± 0.173
4.514IleAsn: 4.514 ± 0.253
2.568IlePro: 2.568 ± 0.203
2.711IleGln: 2.711 ± 0.226
2.839IleArg: 2.839 ± 0.216
4.498IleSer: 4.498 ± 0.312
5.518IleThr: 5.518 ± 0.434
4.147IleVal: 4.147 ± 0.295
0.367IleTrp: 0.367 ± 0.07
2.169IleTyr: 2.169 ± 0.192
0.0IleXaa: 0.0 ± 0.0
Lys
3.397LysAla: 3.397 ± 0.306
0.478LysCys: 0.478 ± 0.094
4.163LysAsp: 4.163 ± 0.366
5.056LysGlu: 5.056 ± 0.535
2.935LysPhe: 2.935 ± 0.217
3.971LysGly: 3.971 ± 0.377
0.957LysHis: 0.957 ± 0.171
5.359LysIle: 5.359 ± 0.313
6.316LysLys: 6.316 ± 0.836
5.726LysLeu: 5.726 ± 0.428
1.802LysMet: 1.802 ± 0.238
3.445LysAsn: 3.445 ± 0.268
2.568LysPro: 2.568 ± 0.205
2.424LysGln: 2.424 ± 0.252
2.807LysArg: 2.807 ± 0.325
4.386LysSer: 4.386 ± 0.349
3.812LysThr: 3.812 ± 0.309
4.498LysVal: 4.498 ± 0.316
0.558LysTrp: 0.558 ± 0.095
3.19LysTyr: 3.19 ± 0.305
0.0LysXaa: 0.0 ± 0.0
Leu
4.386LeuAla: 4.386 ± 0.269
0.67LeuCys: 0.67 ± 0.125
5.455LeuAsp: 5.455 ± 0.354
5.072LeuGlu: 5.072 ± 0.297
3.19LeuPhe: 3.19 ± 0.251
5.47LeuGly: 5.47 ± 0.342
1.451LeuHis: 1.451 ± 0.211
4.115LeuIle: 4.115 ± 0.339
5.47LeuLys: 5.47 ± 0.339
5.04LeuLeu: 5.04 ± 0.41
1.643LeuMet: 1.643 ± 0.188
4.593LeuAsn: 4.593 ± 0.265
3.014LeuPro: 3.014 ± 0.232
2.584LeuGln: 2.584 ± 0.216
3.27LeuArg: 3.27 ± 0.296
5.215LeuSer: 5.215 ± 0.247
5.152LeuThr: 5.152 ± 0.328
4.003LeuVal: 4.003 ± 0.214
0.59LeuTrp: 0.59 ± 0.099
3.142LeuTyr: 3.142 ± 0.227
0.0LeuXaa: 0.0 ± 0.0
Met
1.483MetAla: 1.483 ± 0.166
0.191MetCys: 0.191 ± 0.059
1.132MetAsp: 1.132 ± 0.141
1.196MetGlu: 1.196 ± 0.171
0.925MetPhe: 0.925 ± 0.139
1.324MetGly: 1.324 ± 0.149
0.415MetHis: 0.415 ± 0.099
1.627MetIle: 1.627 ± 0.189
1.754MetLys: 1.754 ± 0.244
1.372MetLeu: 1.372 ± 0.172
0.702MetMet: 0.702 ± 0.137
1.34MetAsn: 1.34 ± 0.164
0.797MetPro: 0.797 ± 0.102
0.75MetGln: 0.75 ± 0.12
1.1MetArg: 1.1 ± 0.151
1.324MetSer: 1.324 ± 0.175
1.547MetThr: 1.547 ± 0.144
1.276MetVal: 1.276 ± 0.152
0.223MetTrp: 0.223 ± 0.063
0.781MetTyr: 0.781 ± 0.115
0.0MetXaa: 0.0 ± 0.0
Asn
3.987AsnAla: 3.987 ± 0.346
0.415AsnCys: 0.415 ± 0.081
3.349AsnAsp: 3.349 ± 0.242
3.285AsnGlu: 3.285 ± 0.242
3.301AsnPhe: 3.301 ± 0.255
4.434AsnGly: 4.434 ± 0.376
1.196AsnHis: 1.196 ± 0.123
4.45AsnIle: 4.45 ± 0.302
3.493AsnLys: 3.493 ± 0.29
4.402AsnLeu: 4.402 ± 0.268
0.973AsnMet: 0.973 ± 0.12
3.397AsnAsn: 3.397 ± 0.316
3.014AsnPro: 3.014 ± 0.256
1.994AsnGln: 1.994 ± 0.161
2.089AsnArg: 2.089 ± 0.166
3.923AsnSer: 3.923 ± 0.257
4.099AsnThr: 4.099 ± 0.316
4.466AsnVal: 4.466 ± 0.316
0.67AsnTrp: 0.67 ± 0.119
2.52AsnTyr: 2.52 ± 0.205
0.0AsnXaa: 0.0 ± 0.0
Pro
1.675ProAla: 1.675 ± 0.174
0.367ProCys: 0.367 ± 0.081
2.982ProAsp: 2.982 ± 0.233
2.504ProGlu: 2.504 ± 0.247
1.994ProPhe: 1.994 ± 0.15
2.313ProGly: 2.313 ± 0.184
0.67ProHis: 0.67 ± 0.093
2.249ProIle: 2.249 ± 0.195
2.536ProLys: 2.536 ± 0.255
2.201ProLeu: 2.201 ± 0.181
0.463ProMet: 0.463 ± 0.09
1.93ProAsn: 1.93 ± 0.216
1.467ProPro: 1.467 ± 0.204
1.372ProGln: 1.372 ± 0.146
1.292ProArg: 1.292 ± 0.139
3.03ProSer: 3.03 ± 0.265
2.967ProThr: 2.967 ± 0.254
2.568ProVal: 2.568 ± 0.244
0.431ProTrp: 0.431 ± 0.083
1.292ProTyr: 1.292 ± 0.165
0.0ProXaa: 0.0 ± 0.0
Gln
1.595GlnAla: 1.595 ± 0.182
0.287GlnCys: 0.287 ± 0.073
2.281GlnAsp: 2.281 ± 0.186
2.392GlnGlu: 2.392 ± 0.242
1.754GlnPhe: 1.754 ± 0.158
2.711GlnGly: 2.711 ± 0.234
0.558GlnHis: 0.558 ± 0.095
2.424GlnIle: 2.424 ± 0.206
2.169GlnLys: 2.169 ± 0.242
2.998GlnLeu: 2.998 ± 0.192
0.941GlnMet: 0.941 ± 0.164
2.026GlnAsn: 2.026 ± 0.174
1.164GlnPro: 1.164 ± 0.131
1.324GlnGln: 1.324 ± 0.166
1.34GlnArg: 1.34 ± 0.145
2.185GlnSer: 2.185 ± 0.192
2.217GlnThr: 2.217 ± 0.215
2.36GlnVal: 2.36 ± 0.163
0.287GlnTrp: 0.287 ± 0.077
1.419GlnTyr: 1.419 ± 0.158
0.0GlnXaa: 0.0 ± 0.0
Arg
2.217ArgAla: 2.217 ± 0.189
0.303ArgCys: 0.303 ± 0.075
2.44ArgAsp: 2.44 ± 0.224
2.217ArgGlu: 2.217 ± 0.213
1.866ArgPhe: 1.866 ± 0.142
2.982ArgGly: 2.982 ± 0.199
0.686ArgHis: 0.686 ± 0.108
3.158ArgIle: 3.158 ± 0.273
3.094ArgLys: 3.094 ± 0.278
3.525ArgLeu: 3.525 ± 0.22
1.148ArgMet: 1.148 ± 0.156
2.073ArgAsn: 2.073 ± 0.177
1.116ArgPro: 1.116 ± 0.167
1.308ArgGln: 1.308 ± 0.172
1.802ArgArg: 1.802 ± 0.245
2.775ArgSer: 2.775 ± 0.189
2.057ArgThr: 2.057 ± 0.203
2.568ArgVal: 2.568 ± 0.223
0.335ArgTrp: 0.335 ± 0.071
2.026ArgTyr: 2.026 ± 0.204
0.0ArgXaa: 0.0 ± 0.0
Ser
4.721SerAla: 4.721 ± 0.368
0.59SerCys: 0.59 ± 0.109
4.625SerAsp: 4.625 ± 0.237
3.557SerGlu: 3.557 ± 0.285
3.509SerPhe: 3.509 ± 0.304
7.448SerGly: 7.448 ± 0.574
1.34SerHis: 1.34 ± 0.127
4.785SerIle: 4.785 ± 0.302
4.45SerLys: 4.45 ± 0.357
4.817SerLeu: 4.817 ± 0.262
1.611SerMet: 1.611 ± 0.189
4.514SerAsn: 4.514 ± 0.354
2.121SerPro: 2.121 ± 0.179
2.249SerGln: 2.249 ± 0.172
2.408SerArg: 2.408 ± 0.187
5.821SerSer: 5.821 ± 0.483
5.104SerThr: 5.104 ± 0.371
4.418SerVal: 4.418 ± 0.317
0.702SerTrp: 0.702 ± 0.112
2.807SerTyr: 2.807 ± 0.241
0.0SerXaa: 0.0 ± 0.0
Thr
5.199ThrAla: 5.199 ± 0.467
0.367ThrCys: 0.367 ± 0.067
4.147ThrAsp: 4.147 ± 0.258
3.62ThrGlu: 3.62 ± 0.263
3.589ThrPhe: 3.589 ± 0.298
7.145ThrGly: 7.145 ± 0.701
0.781ThrHis: 0.781 ± 0.119
4.019ThrIle: 4.019 ± 0.391
4.274ThrLys: 4.274 ± 0.266
5.024ThrLeu: 5.024 ± 0.309
0.893ThrMet: 0.893 ± 0.105
4.466ThrAsn: 4.466 ± 0.387
3.014ThrPro: 3.014 ± 0.209
2.249ThrGln: 2.249 ± 0.183
2.408ThrArg: 2.408 ± 0.183
5.662ThrSer: 5.662 ± 0.462
6.459ThrThr: 6.459 ± 0.581
4.593ThrVal: 4.593 ± 0.392
0.654ThrTrp: 0.654 ± 0.109
2.552ThrTyr: 2.552 ± 0.207
0.0ThrXaa: 0.0 ± 0.0
Val
4.386ValAla: 4.386 ± 0.284
0.686ValCys: 0.686 ± 0.103
4.769ValAsp: 4.769 ± 0.291
3.716ValGlu: 3.716 ± 0.293
2.648ValPhe: 2.648 ± 0.256
5.997ValGly: 5.997 ± 0.577
0.829ValHis: 0.829 ± 0.119
3.541ValIle: 3.541 ± 0.243
4.45ValLys: 4.45 ± 0.373
4.115ValLeu: 4.115 ± 0.247
1.18ValMet: 1.18 ± 0.171
4.019ValAsn: 4.019 ± 0.343
2.408ValPro: 2.408 ± 0.237
2.041ValGln: 2.041 ± 0.166
2.456ValArg: 2.456 ± 0.195
5.455ValSer: 5.455 ± 0.383
5.805ValThr: 5.805 ± 0.465
4.482ValVal: 4.482 ± 0.344
0.861ValTrp: 0.861 ± 0.135
2.392ValTyr: 2.392 ± 0.167
0.0ValXaa: 0.0 ± 0.0
Trp
0.622TrpAla: 0.622 ± 0.136
0.191TrpCys: 0.191 ± 0.065
0.845TrpAsp: 0.845 ± 0.116
0.558TrpGlu: 0.558 ± 0.094
0.431TrpPhe: 0.431 ± 0.088
0.797TrpGly: 0.797 ± 0.124
0.223TrpHis: 0.223 ± 0.057
0.638TrpIle: 0.638 ± 0.095
0.781TrpLys: 0.781 ± 0.117
0.686TrpLeu: 0.686 ± 0.128
0.447TrpMet: 0.447 ± 0.085
0.702TrpAsn: 0.702 ± 0.13
0.207TrpPro: 0.207 ± 0.051
0.383TrpGln: 0.383 ± 0.084
0.51TrpArg: 0.51 ± 0.099
0.686TrpSer: 0.686 ± 0.122
0.526TrpThr: 0.526 ± 0.095
0.67TrpVal: 0.67 ± 0.107
0.096TrpTrp: 0.096 ± 0.035
0.367TrpTyr: 0.367 ± 0.076
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.36TyrAla: 2.36 ± 0.172
0.654TyrCys: 0.654 ± 0.121
2.967TyrAsp: 2.967 ± 0.226
2.807TyrGlu: 2.807 ± 0.251
1.786TyrPhe: 1.786 ± 0.18
2.472TyrGly: 2.472 ± 0.201
1.005TyrHis: 1.005 ± 0.136
2.632TyrIle: 2.632 ± 0.242
2.552TyrLys: 2.552 ± 0.22
2.807TyrLeu: 2.807 ± 0.254
0.766TyrMet: 0.766 ± 0.12
2.552TyrAsn: 2.552 ± 0.189
1.627TyrPro: 1.627 ± 0.178
1.435TyrGln: 1.435 ± 0.146
1.691TyrArg: 1.691 ± 0.175
2.536TyrSer: 2.536 ± 0.178
2.376TyrThr: 2.376 ± 0.228
2.552TyrVal: 2.552 ± 0.173
0.574TyrTrp: 0.574 ± 0.118
2.233TyrTyr: 2.233 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 246 proteins (62701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski