Amino acid dipepetide frequency for Paenibacillus sp. 7541

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.401AlaAla: 9.401 ± 0.12
0.724AlaCys: 0.724 ± 0.022
4.518AlaAsp: 4.518 ± 0.065
6.077AlaGlu: 6.077 ± 0.08
3.342AlaPhe: 3.342 ± 0.045
7.093AlaGly: 7.093 ± 0.074
1.515AlaHis: 1.515 ± 0.033
5.136AlaIle: 5.136 ± 0.059
3.749AlaLys: 3.749 ± 0.057
8.615AlaLeu: 8.615 ± 0.097
2.445AlaMet: 2.445 ± 0.044
2.439AlaAsn: 2.439 ± 0.043
2.863AlaPro: 2.863 ± 0.045
2.781AlaGln: 2.781 ± 0.04
3.956AlaArg: 3.956 ± 0.051
5.318AlaSer: 5.318 ± 0.072
3.414AlaThr: 3.414 ± 0.053
6.839AlaVal: 6.839 ± 0.075
1.096AlaTrp: 1.096 ± 0.031
2.821AlaTyr: 2.821 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.536CysAla: 0.536 ± 0.02
0.097CysCys: 0.097 ± 0.007
0.374CysAsp: 0.374 ± 0.016
0.403CysGlu: 0.403 ± 0.014
0.271CysPhe: 0.271 ± 0.013
0.768CysGly: 0.768 ± 0.023
0.178CysHis: 0.178 ± 0.011
0.491CysIle: 0.491 ± 0.02
0.27CysLys: 0.27 ± 0.014
0.723CysLeu: 0.723 ± 0.023
0.208CysMet: 0.208 ± 0.011
0.225CysAsn: 0.225 ± 0.013
0.359CysPro: 0.359 ± 0.015
0.229CysGln: 0.229 ± 0.012
0.479CysArg: 0.479 ± 0.019
0.526CysSer: 0.526 ± 0.017
0.401CysThr: 0.401 ± 0.018
0.42CysVal: 0.42 ± 0.02
0.085CysTrp: 0.085 ± 0.008
0.23CysTyr: 0.23 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
3.942AspAla: 3.942 ± 0.052
0.359AspCys: 0.359 ± 0.014
2.451AspAsp: 2.451 ± 0.046
3.903AspGlu: 3.903 ± 0.053
2.03AspPhe: 2.03 ± 0.038
4.142AspGly: 4.142 ± 0.072
1.257AspHis: 1.257 ± 0.035
3.685AspIle: 3.685 ± 0.054
2.374AspLys: 2.374 ± 0.044
4.669AspLeu: 4.669 ± 0.052
1.54AspMet: 1.54 ± 0.035
1.787AspAsn: 1.787 ± 0.035
2.565AspPro: 2.565 ± 0.046
2.017AspGln: 2.017 ± 0.039
3.057AspArg: 3.057 ± 0.048
2.705AspSer: 2.705 ± 0.042
2.534AspThr: 2.534 ± 0.044
3.681AspVal: 3.681 ± 0.049
0.797AspTrp: 0.797 ± 0.024
2.145AspTyr: 2.145 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
6.683GluAla: 6.683 ± 0.085
0.374GluCys: 0.374 ± 0.017
3.45GluAsp: 3.45 ± 0.053
5.87GluGlu: 5.87 ± 0.083
2.166GluPhe: 2.166 ± 0.041
4.941GluGly: 4.941 ± 0.063
1.717GluHis: 1.717 ± 0.033
4.222GluIle: 4.222 ± 0.06
3.498GluLys: 3.498 ± 0.053
7.473GluLeu: 7.473 ± 0.078
2.179GluMet: 2.179 ± 0.043
2.23GluAsn: 2.23 ± 0.039
2.605GluPro: 2.605 ± 0.048
3.94GluGln: 3.94 ± 0.057
4.366GluArg: 4.366 ± 0.062
3.441GluSer: 3.441 ± 0.051
3.267GluThr: 3.267 ± 0.046
4.694GluVal: 4.694 ± 0.055
0.968GluTrp: 0.968 ± 0.026
2.034GluTyr: 2.034 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.072PheAla: 3.072 ± 0.041
0.326PheCys: 0.326 ± 0.017
2.092PheAsp: 2.092 ± 0.036
2.343PheGlu: 2.343 ± 0.046
1.73PhePhe: 1.73 ± 0.04
3.123PheGly: 3.123 ± 0.046
0.923PheHis: 0.923 ± 0.028
2.845PheIle: 2.845 ± 0.059
1.747PheLys: 1.747 ± 0.034
3.714PheLeu: 3.714 ± 0.059
1.227PheMet: 1.227 ± 0.032
1.494PheAsn: 1.494 ± 0.036
1.553PhePro: 1.553 ± 0.032
1.4PheGln: 1.4 ± 0.03
2.172PheArg: 2.172 ± 0.039
2.643PheSer: 2.643 ± 0.04
2.398PheThr: 2.398 ± 0.041
2.766PheVal: 2.766 ± 0.05
0.531PheTrp: 0.531 ± 0.02
1.437PheTyr: 1.437 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
5.979GlyAla: 5.979 ± 0.077
0.685GlyCys: 0.685 ± 0.021
3.564GlyAsp: 3.564 ± 0.06
4.888GlyGlu: 4.888 ± 0.068
3.131GlyPhe: 3.131 ± 0.044
5.859GlyGly: 5.859 ± 0.069
1.608GlyHis: 1.608 ± 0.034
5.53GlyIle: 5.53 ± 0.064
4.027GlyLys: 4.027 ± 0.054
7.403GlyLeu: 7.403 ± 0.074
2.555GlyMet: 2.555 ± 0.04
2.615GlyAsn: 2.615 ± 0.051
2.195GlyPro: 2.195 ± 0.04
2.933GlyGln: 2.933 ± 0.042
3.947GlyArg: 3.947 ± 0.052
5.0GlySer: 5.0 ± 0.071
4.476GlyThr: 4.476 ± 0.061
5.338GlyVal: 5.338 ± 0.053
1.131GlyTrp: 1.131 ± 0.029
3.115GlyTyr: 3.115 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.761HisAla: 1.761 ± 0.038
0.197HisCys: 0.197 ± 0.011
1.093HisAsp: 1.093 ± 0.026
1.43HisGlu: 1.43 ± 0.032
0.977HisPhe: 0.977 ± 0.029
1.613HisGly: 1.613 ± 0.037
0.67HisHis: 0.67 ± 0.026
1.496HisIle: 1.496 ± 0.032
0.848HisLys: 0.848 ± 0.022
2.1HisLeu: 2.1 ± 0.041
0.641HisMet: 0.641 ± 0.019
0.74HisAsn: 0.74 ± 0.021
1.336HisPro: 1.336 ± 0.03
0.893HisGln: 0.893 ± 0.025
1.271HisArg: 1.271 ± 0.031
1.192HisSer: 1.192 ± 0.029
1.13HisThr: 1.13 ± 0.028
1.535HisVal: 1.535 ± 0.032
0.327HisTrp: 0.327 ± 0.014
0.896HisTyr: 0.896 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.774IleAla: 5.774 ± 0.074
0.538IleCys: 0.538 ± 0.019
3.45IleAsp: 3.45 ± 0.044
4.282IleGlu: 4.282 ± 0.063
2.313IlePhe: 2.313 ± 0.045
5.254IleGly: 5.254 ± 0.06
1.583IleHis: 1.583 ± 0.032
4.078IleIle: 4.078 ± 0.067
2.667IleLys: 2.667 ± 0.04
5.631IleLeu: 5.631 ± 0.075
1.751IleMet: 1.751 ± 0.036
2.135IleAsn: 2.135 ± 0.038
3.275IlePro: 3.275 ± 0.048
2.579IleGln: 2.579 ± 0.038
3.972IleArg: 3.972 ± 0.056
4.318IleSer: 4.318 ± 0.056
3.785IleThr: 3.785 ± 0.059
5.066IleVal: 5.066 ± 0.065
0.732IleTrp: 0.732 ± 0.024
2.095IleTyr: 2.095 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
3.904LysAla: 3.904 ± 0.05
0.21LysCys: 0.21 ± 0.011
2.641LysAsp: 2.641 ± 0.043
3.979LysGlu: 3.979 ± 0.057
1.38LysPhe: 1.38 ± 0.032
3.309LysGly: 3.309 ± 0.048
1.061LysHis: 1.061 ± 0.028
2.637LysIle: 2.637 ± 0.048
2.619LysLys: 2.619 ± 0.049
5.005LysLeu: 5.005 ± 0.061
1.377LysMet: 1.377 ± 0.029
1.648LysAsn: 1.648 ± 0.036
2.163LysPro: 2.163 ± 0.036
2.271LysGln: 2.271 ± 0.042
2.814LysArg: 2.814 ± 0.043
2.645LysSer: 2.645 ± 0.043
2.377LysThr: 2.377 ± 0.045
3.279LysVal: 3.279 ± 0.052
0.677LysTrp: 0.677 ± 0.024
1.589LysTyr: 1.589 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
8.576LeuAla: 8.576 ± 0.095
0.753LeuCys: 0.753 ± 0.022
5.249LeuAsp: 5.249 ± 0.057
6.5LeuGlu: 6.5 ± 0.065
4.289LeuPhe: 4.289 ± 0.077
7.064LeuGly: 7.064 ± 0.077
2.2LeuHis: 2.2 ± 0.039
6.511LeuIle: 6.511 ± 0.085
4.961LeuLys: 4.961 ± 0.056
11.271LeuLeu: 11.271 ± 0.151
2.797LeuMet: 2.797 ± 0.045
3.694LeuAsn: 3.694 ± 0.047
4.617LeuPro: 4.617 ± 0.061
3.968LeuGln: 3.968 ± 0.053
5.15LeuArg: 5.15 ± 0.062
7.045LeuSer: 7.045 ± 0.082
5.416LeuThr: 5.416 ± 0.064
6.516LeuVal: 6.516 ± 0.069
1.085LeuTrp: 1.085 ± 0.028
3.346LeuTyr: 3.346 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.382MetAla: 2.382 ± 0.043
0.16MetCys: 0.16 ± 0.01
1.598MetAsp: 1.598 ± 0.031
2.094MetGlu: 2.094 ± 0.039
1.086MetPhe: 1.086 ± 0.03
1.803MetGly: 1.803 ± 0.034
0.506MetHis: 0.506 ± 0.019
2.015MetIle: 2.015 ± 0.038
2.015MetLys: 2.015 ± 0.04
3.224MetLeu: 3.224 ± 0.052
1.026MetMet: 1.026 ± 0.028
1.557MetAsn: 1.557 ± 0.029
1.208MetPro: 1.208 ± 0.029
1.046MetGln: 1.046 ± 0.026
1.419MetArg: 1.419 ± 0.033
1.954MetSer: 1.954 ± 0.041
1.788MetThr: 1.788 ± 0.031
1.923MetVal: 1.923 ± 0.033
0.256MetTrp: 0.256 ± 0.013
0.802MetTyr: 0.802 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
2.695AsnAla: 2.695 ± 0.05
0.223AsnCys: 0.223 ± 0.013
1.719AsnAsp: 1.719 ± 0.035
2.422AsnGlu: 2.422 ± 0.044
1.204AsnPhe: 1.204 ± 0.031
3.006AsnGly: 3.006 ± 0.052
0.798AsnHis: 0.798 ± 0.024
2.197AsnIle: 2.197 ± 0.037
1.696AsnLys: 1.696 ± 0.04
3.104AsnLeu: 3.104 ± 0.046
1.051AsnMet: 1.051 ± 0.028
1.404AsnAsn: 1.404 ± 0.038
2.06AsnPro: 2.06 ± 0.041
1.397AsnGln: 1.397 ± 0.032
2.07AsnArg: 2.07 ± 0.034
1.947AsnSer: 1.947 ± 0.036
1.898AsnThr: 1.898 ± 0.037
2.463AsnVal: 2.463 ± 0.042
0.542AsnTrp: 0.542 ± 0.02
1.285AsnTyr: 1.285 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
3.623ProAla: 3.623 ± 0.058
0.239ProCys: 0.239 ± 0.014
2.873ProAsp: 2.873 ± 0.049
3.904ProGlu: 3.904 ± 0.057
1.843ProPhe: 1.843 ± 0.034
3.446ProGly: 3.446 ± 0.056
0.992ProHis: 0.992 ± 0.025
2.4ProIle: 2.4 ± 0.045
1.717ProLys: 1.717 ± 0.034
4.032ProLeu: 4.032 ± 0.058
1.025ProMet: 1.025 ± 0.024
1.479ProAsn: 1.479 ± 0.033
1.492ProPro: 1.492 ± 0.038
1.438ProGln: 1.438 ± 0.029
1.622ProArg: 1.622 ± 0.03
2.763ProSer: 2.763 ± 0.04
1.768ProThr: 1.768 ± 0.036
3.555ProVal: 3.555 ± 0.048
0.573ProTrp: 0.573 ± 0.023
1.601ProTyr: 1.601 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.661GlnAla: 3.661 ± 0.048
0.227GlnCys: 0.227 ± 0.012
1.882GlnAsp: 1.882 ± 0.038
2.956GlnGlu: 2.956 ± 0.047
1.428GlnPhe: 1.428 ± 0.032
2.827GlnGly: 2.827 ± 0.037
0.925GlnHis: 0.925 ± 0.027
2.355GlnIle: 2.355 ± 0.039
1.7GlnLys: 1.7 ± 0.034
4.373GlnLeu: 4.373 ± 0.064
1.19GlnMet: 1.19 ± 0.027
1.214GlnAsn: 1.214 ± 0.028
1.687GlnPro: 1.687 ± 0.032
2.024GlnGln: 2.024 ± 0.039
2.152GlnArg: 2.152 ± 0.039
2.218GlnSer: 2.218 ± 0.036
1.933GlnThr: 1.933 ± 0.038
2.597GlnVal: 2.597 ± 0.042
0.553GlnTrp: 0.553 ± 0.018
1.389GlnTyr: 1.389 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
3.576ArgAla: 3.576 ± 0.055
0.404ArgCys: 0.404 ± 0.017
2.641ArgAsp: 2.641 ± 0.042
4.164ArgGlu: 4.164 ± 0.067
2.264ArgPhe: 2.264 ± 0.038
3.244ArgGly: 3.244 ± 0.043
1.264ArgHis: 1.264 ± 0.031
3.853ArgIle: 3.853 ± 0.051
3.077ArgLys: 3.077 ± 0.049
5.841ArgLeu: 5.841 ± 0.085
1.873ArgMet: 1.873 ± 0.038
2.035ArgAsn: 2.035 ± 0.039
1.952ArgPro: 1.952 ± 0.041
2.373ArgGln: 2.373 ± 0.04
3.188ArgArg: 3.188 ± 0.057
3.425ArgSer: 3.425 ± 0.042
2.856ArgThr: 2.856 ± 0.042
3.392ArgVal: 3.392 ± 0.044
0.783ArgTrp: 0.783 ± 0.025
2.149ArgTyr: 2.149 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
4.936SerAla: 4.936 ± 0.061
0.402SerCys: 0.402 ± 0.017
3.013SerAsp: 3.013 ± 0.055
3.908SerGlu: 3.908 ± 0.048
2.913SerPhe: 2.913 ± 0.044
5.737SerGly: 5.737 ± 0.067
1.233SerHis: 1.233 ± 0.032
4.091SerIle: 4.091 ± 0.055
2.721SerLys: 2.721 ± 0.043
6.286SerLeu: 6.286 ± 0.07
1.938SerMet: 1.938 ± 0.036
2.017SerAsn: 2.017 ± 0.042
2.706SerPro: 2.706 ± 0.039
2.02SerGln: 2.02 ± 0.039
3.525SerArg: 3.525 ± 0.052
4.283SerSer: 4.283 ± 0.061
3.025SerThr: 3.025 ± 0.051
4.432SerVal: 4.432 ± 0.061
0.846SerTrp: 0.846 ± 0.026
2.245SerTyr: 2.245 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
4.748ThrAla: 4.748 ± 0.059
0.338ThrCys: 0.338 ± 0.014
2.772ThrAsp: 2.772 ± 0.048
3.283ThrGlu: 3.283 ± 0.044
2.171ThrPhe: 2.171 ± 0.034
4.615ThrGly: 4.615 ± 0.061
1.023ThrHis: 1.023 ± 0.025
3.607ThrIle: 3.607 ± 0.051
2.086ThrLys: 2.086 ± 0.036
5.238ThrLeu: 5.238 ± 0.054
1.398ThrMet: 1.398 ± 0.035
1.646ThrAsn: 1.646 ± 0.039
2.507ThrPro: 2.507 ± 0.044
1.502ThrGln: 1.502 ± 0.033
2.44ThrArg: 2.44 ± 0.039
3.214ThrSer: 3.214 ± 0.054
2.616ThrThr: 2.616 ± 0.048
4.374ThrVal: 4.374 ± 0.06
0.644ThrTrp: 0.644 ± 0.019
1.867ThrTyr: 1.867 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
5.245ValAla: 5.245 ± 0.058
0.608ValCys: 0.608 ± 0.018
3.643ValAsp: 3.643 ± 0.055
4.436ValGlu: 4.436 ± 0.056
2.886ValPhe: 2.886 ± 0.051
4.498ValGly: 4.498 ± 0.061
1.56ValHis: 1.56 ± 0.033
4.992ValIle: 4.992 ± 0.068
3.619ValLys: 3.619 ± 0.056
7.494ValLeu: 7.494 ± 0.074
2.186ValMet: 2.186 ± 0.039
2.797ValAsn: 2.797 ± 0.05
3.223ValPro: 3.223 ± 0.049
2.788ValGln: 2.788 ± 0.042
3.774ValArg: 3.774 ± 0.055
4.7ValSer: 4.7 ± 0.057
4.33ValThr: 4.33 ± 0.066
5.195ValVal: 5.195 ± 0.068
0.871ValTrp: 0.871 ± 0.024
2.516ValTyr: 2.516 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.869TrpAla: 0.869 ± 0.025
0.105TrpCys: 0.105 ± 0.008
0.674TrpAsp: 0.674 ± 0.019
0.818TrpGlu: 0.818 ± 0.025
0.557TrpPhe: 0.557 ± 0.021
0.9TrpGly: 0.9 ± 0.029
0.292TrpHis: 0.292 ± 0.014
0.938TrpIle: 0.938 ± 0.027
0.68TrpLys: 0.68 ± 0.021
1.507TrpLeu: 1.507 ± 0.037
0.478TrpMet: 0.478 ± 0.018
0.701TrpAsn: 0.701 ± 0.021
0.408TrpPro: 0.408 ± 0.017
0.476TrpGln: 0.476 ± 0.018
0.687TrpArg: 0.687 ± 0.02
0.872TrpSer: 0.872 ± 0.025
0.723TrpThr: 0.723 ± 0.027
0.853TrpVal: 0.853 ± 0.025
0.176TrpTrp: 0.176 ± 0.01
0.407TrpTyr: 0.407 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.776TyrAla: 2.776 ± 0.043
0.305TyrCys: 0.305 ± 0.014
1.944TyrAsp: 1.944 ± 0.035
2.454TyrGlu: 2.454 ± 0.043
1.556TyrPhe: 1.556 ± 0.031
2.739TyrGly: 2.739 ± 0.044
0.84TyrHis: 0.84 ± 0.025
2.199TyrIle: 2.199 ± 0.039
1.514TyrLys: 1.514 ± 0.033
3.302TyrLeu: 3.302 ± 0.056
0.977TyrMet: 0.977 ± 0.024
1.322TyrAsn: 1.322 ± 0.031
1.612TyrPro: 1.612 ± 0.033
1.215TyrGln: 1.215 ± 0.029
2.293TyrArg: 2.293 ± 0.037
2.07TyrSer: 2.07 ± 0.039
1.954TyrThr: 1.954 ± 0.039
2.445TyrVal: 2.445 ± 0.04
0.45TyrTrp: 0.45 ± 0.018
1.408TyrTyr: 1.408 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4741 proteins (1530362 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski