Escherichia phage vB_EcoP_F

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; Escherichia virus F

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6KVI2|A0A1S6KVI2_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_F OX=1933110 GN=F_31 PE=4 SV=1
MM1 pKa = 6.43VTVILVLTLISGSGQTISQVPITAPTLQDD30 pKa = 3.28AFTICDD36 pKa = 3.33QSGSKK41 pKa = 9.17WADD44 pKa = 2.98KK45 pKa = 10.92FGDD48 pKa = 3.73VSNVAAGYY56 pKa = 10.06EE57 pKa = 4.52CII59 pKa = 4.8

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6KVG0|A0A1S6KVG0_9CAUD DNA mimic ocr OS=Escherichia phage vB_EcoP_F OX=1933110 GN=F_10 PE=4 SV=1
MM1 pKa = 7.53SSAILIAIGYY11 pKa = 8.51GLIAYY16 pKa = 8.1VLVRR20 pKa = 11.84DD21 pKa = 3.27IDD23 pKa = 3.79KK24 pKa = 10.87ARR26 pKa = 11.84KK27 pKa = 8.48VYY29 pKa = 10.05KK30 pKa = 10.33FNYY33 pKa = 8.37VRR35 pKa = 11.84LGRR38 pKa = 11.84WTVRR42 pKa = 11.84QPNGRR47 pKa = 11.84FMRR50 pKa = 11.84NLANVWDD57 pKa = 3.9IATIGSKK64 pKa = 10.35LL65 pKa = 3.44

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

10797

30

1064

224.9

25.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.558 ± 0.466

0.926 ± 0.133

6.631 ± 0.244

6.863 ± 0.412

3.668 ± 0.201

7.669 ± 0.317

1.871 ± 0.222

5.048 ± 0.188

6.391 ± 0.432

7.715 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.834 ± 0.214

4.288 ± 0.267

3.77 ± 0.186

3.89 ± 0.449

5.27 ± 0.262

6.437 ± 0.362

5.603 ± 0.288

7.326 ± 0.279

1.528 ± 0.16

3.714 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski