Streptomyces corynorhini
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6445 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A370BDE7|A0A370BDE7_9ACTN Roadblock/LC7 domain-containing protein OS=Streptomyces corynorhini OX=2282652 GN=DVH02_02670 PE=4 SV=1
MM1 pKa = 7.96 SYY3 pKa = 10.02 PQQLRR8 pKa = 11.84 YY9 pKa = 10.24 SKK11 pKa = 9.49 EE12 pKa = 4.17 HH13 pKa = 6.0 EE14 pKa = 4.24 WLSAADD20 pKa = 3.83 NGVSTVGITEE30 pKa = 4.0 YY31 pKa = 10.99 AANALGDD38 pKa = 3.79 VVFAQLPEE46 pKa = 4.02 VGEE49 pKa = 4.32 TVTAGEE55 pKa = 4.43 TCGEE59 pKa = 4.13 LEE61 pKa = 4.31 STKK64 pKa = 10.61 SVSDD68 pKa = 4.05 LYY70 pKa = 11.58 SPVTGEE76 pKa = 4.04 VTEE79 pKa = 4.4 TNQDD83 pKa = 3.48 VVDD86 pKa = 5.17 DD87 pKa = 4.35 PSLVNSAPYY96 pKa = 9.91 EE97 pKa = 4.36 GGWLFKK103 pKa = 11.07 VRR105 pKa = 11.84 VEE107 pKa = 5.11 AEE109 pKa = 4.34 DD110 pKa = 3.9 EE111 pKa = 4.12 LLSADD116 pKa = 4.0 EE117 pKa = 4.21 YY118 pKa = 11.7 KK119 pKa = 10.84 EE120 pKa = 3.9 FSGNN124 pKa = 3.2
Molecular weight: 13.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.91
IPC_protein 3.821
Toseland 3.656
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.668
Grimsley 3.567
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.024
Thurlkill 3.681
EMBOSS 3.681
Sillero 3.935
Patrickios 1.85
IPC_peptide 3.77
IPC2_peptide 3.923
IPC2.peptide.svr19 3.853
Protein with the highest isoelectric point:
>tr|A0A370B5A8|A0A370B5A8_9ACTN TetR/AcrR family transcriptional regulator OS=Streptomyces corynorhini OX=2282652 GN=DVH02_18375 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILATRR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.48 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6445
0
6445
2146243
18
3943
333.0
35.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.814 ± 0.043
0.764 ± 0.007
5.938 ± 0.025
5.642 ± 0.034
2.717 ± 0.017
9.769 ± 0.031
2.216 ± 0.013
3.118 ± 0.022
2.086 ± 0.027
10.274 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.683 ± 0.013
1.704 ± 0.017
6.162 ± 0.028
2.575 ± 0.015
8.229 ± 0.035
5.236 ± 0.026
6.182 ± 0.024
8.357 ± 0.025
1.474 ± 0.013
2.057 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here