Amino acid dipepetide frequency for bacterium D16-54

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.185AlaAla: 8.185 ± 0.125
1.129AlaCys: 1.129 ± 0.029
4.265AlaAsp: 4.265 ± 0.066
5.724AlaGlu: 5.724 ± 0.076
2.997AlaPhe: 2.997 ± 0.053
6.745AlaGly: 6.745 ± 0.095
1.017AlaHis: 1.017 ± 0.025
4.305AlaIle: 4.305 ± 0.062
4.009AlaLys: 4.009 ± 0.053
6.647AlaLeu: 6.647 ± 0.075
2.516AlaMet: 2.516 ± 0.043
2.292AlaAsn: 2.292 ± 0.047
2.094AlaPro: 2.094 ± 0.05
2.403AlaGln: 2.403 ± 0.051
3.489AlaArg: 3.489 ± 0.048
3.939AlaSer: 3.939 ± 0.056
2.547AlaThr: 2.547 ± 0.047
6.411AlaVal: 6.411 ± 0.071
0.778AlaTrp: 0.778 ± 0.021
2.77AlaTyr: 2.77 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.947CysAla: 0.947 ± 0.024
0.331CysCys: 0.331 ± 0.016
0.777CysAsp: 0.777 ± 0.025
0.874CysGlu: 0.874 ± 0.023
0.76CysPhe: 0.76 ± 0.026
1.555CysGly: 1.555 ± 0.033
0.339CysHis: 0.339 ± 0.018
1.011CysIle: 1.011 ± 0.028
0.784CysLys: 0.784 ± 0.022
1.372CysLeu: 1.372 ± 0.032
0.469CysMet: 0.469 ± 0.017
0.529CysAsn: 0.529 ± 0.021
0.67CysPro: 0.67 ± 0.023
0.572CysGln: 0.572 ± 0.02
1.097CysArg: 1.097 ± 0.034
1.015CysSer: 1.015 ± 0.025
0.706CysThr: 0.706 ± 0.021
1.014CysVal: 1.014 ± 0.029
0.148CysTrp: 0.148 ± 0.009
0.621CysTyr: 0.621 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.54AspAla: 3.54 ± 0.058
0.89AspCys: 0.89 ± 0.025
2.59AspAsp: 2.59 ± 0.046
4.185AspGlu: 4.185 ± 0.065
2.764AspPhe: 2.764 ± 0.048
4.884AspGly: 4.884 ± 0.077
0.94AspHis: 0.94 ± 0.025
3.99AspIle: 3.99 ± 0.057
3.271AspLys: 3.271 ± 0.052
4.548AspLeu: 4.548 ± 0.052
1.834AspMet: 1.834 ± 0.037
2.048AspAsn: 2.048 ± 0.043
1.778AspPro: 1.778 ± 0.041
1.702AspGln: 1.702 ± 0.038
2.93AspArg: 2.93 ± 0.049
3.324AspSer: 3.324 ± 0.053
2.86AspThr: 2.86 ± 0.046
3.403AspVal: 3.403 ± 0.048
0.714AspTrp: 0.714 ± 0.022
2.758AspTyr: 2.758 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
5.713GluAla: 5.713 ± 0.078
0.881GluCys: 0.881 ± 0.025
4.789GluAsp: 4.789 ± 0.068
7.143GluGlu: 7.143 ± 0.087
2.541GluPhe: 2.541 ± 0.038
4.864GluGly: 4.864 ± 0.068
1.348GluHis: 1.348 ± 0.028
5.338GluIle: 5.338 ± 0.072
6.503GluLys: 6.503 ± 0.083
6.978GluLeu: 6.978 ± 0.076
2.391GluMet: 2.391 ± 0.039
3.997GluAsn: 3.997 ± 0.054
2.181GluPro: 2.181 ± 0.048
3.312GluGln: 3.312 ± 0.058
4.453GluArg: 4.453 ± 0.066
3.518GluSer: 3.518 ± 0.064
3.766GluThr: 3.766 ± 0.06
3.921GluVal: 3.921 ± 0.056
0.818GluTrp: 0.818 ± 0.025
3.177GluTyr: 3.177 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
2.87PheAla: 2.87 ± 0.049
0.906PheCys: 0.906 ± 0.029
2.42PheAsp: 2.42 ± 0.036
2.545PheGlu: 2.545 ± 0.048
1.976PhePhe: 1.976 ± 0.045
3.115PheGly: 3.115 ± 0.055
0.901PheHis: 0.901 ± 0.026
2.443PheIle: 2.443 ± 0.044
1.744PheLys: 1.744 ± 0.038
4.367PheLeu: 4.367 ± 0.07
1.212PheMet: 1.212 ± 0.03
1.334PheAsn: 1.334 ± 0.033
1.523PhePro: 1.523 ± 0.037
1.557PheGln: 1.557 ± 0.031
1.99PheArg: 1.99 ± 0.038
3.045PheSer: 3.045 ± 0.047
2.107PheThr: 2.107 ± 0.046
2.734PheVal: 2.734 ± 0.045
0.519PheTrp: 0.519 ± 0.02
1.833PheTyr: 1.833 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
5.169GlyAla: 5.169 ± 0.08
1.297GlyCys: 1.297 ± 0.031
3.634GlyAsp: 3.634 ± 0.051
5.386GlyGlu: 5.386 ± 0.066
3.198GlyPhe: 3.198 ± 0.048
5.377GlyGly: 5.377 ± 0.097
1.297GlyHis: 1.297 ± 0.032
6.242GlyIle: 6.242 ± 0.089
5.521GlyLys: 5.521 ± 0.059
6.019GlyLeu: 6.019 ± 0.067
2.774GlyMet: 2.774 ± 0.048
3.332GlyAsn: 3.332 ± 0.057
1.321GlyPro: 1.321 ± 0.031
2.658GlyGln: 2.658 ± 0.047
4.11GlyArg: 4.11 ± 0.06
4.415GlySer: 4.415 ± 0.071
4.053GlyThr: 4.053 ± 0.067
4.905GlyVal: 4.905 ± 0.06
0.936GlyTrp: 0.936 ± 0.028
3.317GlyTyr: 3.317 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.108HisAla: 1.108 ± 0.028
0.323HisCys: 0.323 ± 0.015
0.882HisAsp: 0.882 ± 0.025
1.082HisGlu: 1.082 ± 0.029
0.88HisPhe: 0.88 ± 0.028
1.37HisGly: 1.37 ± 0.035
0.406HisHis: 0.406 ± 0.019
1.378HisIle: 1.378 ± 0.032
0.931HisLys: 0.931 ± 0.024
1.523HisLeu: 1.523 ± 0.037
0.563HisMet: 0.563 ± 0.021
0.682HisAsn: 0.682 ± 0.022
0.862HisPro: 0.862 ± 0.024
0.621HisGln: 0.621 ± 0.021
0.931HisArg: 0.931 ± 0.025
1.055HisSer: 1.055 ± 0.027
0.96HisThr: 0.96 ± 0.023
1.123HisVal: 1.123 ± 0.024
0.216HisTrp: 0.216 ± 0.012
0.825HisTyr: 0.825 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
4.807IleAla: 4.807 ± 0.058
1.308IleCys: 1.308 ± 0.034
3.469IleAsp: 3.469 ± 0.055
4.361IleGlu: 4.361 ± 0.055
2.789IlePhe: 2.789 ± 0.051
4.741IleGly: 4.741 ± 0.062
1.507IleHis: 1.507 ± 0.03
4.282IleIle: 4.282 ± 0.068
3.53IleLys: 3.53 ± 0.058
6.917IleLeu: 6.917 ± 0.086
1.826IleMet: 1.826 ± 0.043
2.535IleAsn: 2.535 ± 0.045
3.074IlePro: 3.074 ± 0.038
2.624IleGln: 2.624 ± 0.042
4.211IleArg: 4.211 ± 0.06
4.697IleSer: 4.697 ± 0.061
3.694IleThr: 3.694 ± 0.053
4.275IleVal: 4.275 ± 0.055
0.703IleTrp: 0.703 ± 0.022
2.757IleTyr: 2.757 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
4.807LysAla: 4.807 ± 0.063
0.68LysCys: 0.68 ± 0.022
3.461LysAsp: 3.461 ± 0.05
6.223LysGlu: 6.223 ± 0.077
1.553LysPhe: 1.553 ± 0.035
4.337LysGly: 4.337 ± 0.062
0.948LysHis: 0.948 ± 0.025
4.222LysIle: 4.222 ± 0.058
5.533LysLys: 5.533 ± 0.072
4.95LysLeu: 4.95 ± 0.062
1.861LysMet: 1.861 ± 0.034
3.203LysAsn: 3.203 ± 0.053
2.0LysPro: 2.0 ± 0.037
2.333LysGln: 2.333 ± 0.044
3.612LysArg: 3.612 ± 0.052
3.154LysSer: 3.154 ± 0.05
3.266LysThr: 3.266 ± 0.044
3.656LysVal: 3.656 ± 0.055
0.682LysTrp: 0.682 ± 0.022
2.527LysTyr: 2.527 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
7.038LeuAla: 7.038 ± 0.079
1.614LeuCys: 1.614 ± 0.036
5.047LeuAsp: 5.047 ± 0.058
6.944LeuGlu: 6.944 ± 0.071
3.903LeuPhe: 3.903 ± 0.063
5.908LeuGly: 5.908 ± 0.068
1.561LeuHis: 1.561 ± 0.037
5.505LeuIle: 5.505 ± 0.068
5.906LeuLys: 5.906 ± 0.061
8.725LeuLeu: 8.725 ± 0.107
2.745LeuMet: 2.745 ± 0.044
3.569LeuAsn: 3.569 ± 0.053
3.529LeuPro: 3.529 ± 0.052
3.013LeuGln: 3.013 ± 0.045
4.205LeuArg: 4.205 ± 0.055
6.248LeuSer: 6.248 ± 0.064
4.876LeuThr: 4.876 ± 0.057
5.507LeuVal: 5.507 ± 0.069
0.963LeuTrp: 0.963 ± 0.031
3.603LeuTyr: 3.603 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.761MetAla: 2.761 ± 0.045
0.367MetCys: 0.367 ± 0.015
1.996MetAsp: 1.996 ± 0.041
2.87MetGlu: 2.87 ± 0.046
1.008MetPhe: 1.008 ± 0.027
2.334MetGly: 2.334 ± 0.04
0.37MetHis: 0.37 ± 0.016
2.109MetIle: 2.109 ± 0.04
2.392MetLys: 2.392 ± 0.043
2.7MetLeu: 2.7 ± 0.044
0.988MetMet: 0.988 ± 0.028
1.463MetAsn: 1.463 ± 0.033
1.17MetPro: 1.17 ± 0.027
0.984MetGln: 0.984 ± 0.025
1.515MetArg: 1.515 ± 0.034
1.699MetSer: 1.699 ± 0.041
1.676MetThr: 1.676 ± 0.035
2.14MetVal: 2.14 ± 0.038
0.241MetTrp: 0.241 ± 0.013
0.863MetTyr: 0.863 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.717AsnAla: 2.717 ± 0.045
0.597AsnCys: 0.597 ± 0.02
1.829AsnAsp: 1.829 ± 0.034
2.485AsnGlu: 2.485 ± 0.04
1.481AsnPhe: 1.481 ± 0.03
3.51AsnGly: 3.51 ± 0.055
0.901AsnHis: 0.901 ± 0.025
2.933AsnIle: 2.933 ± 0.047
2.056AsnLys: 2.056 ± 0.043
3.692AsnLeu: 3.692 ± 0.054
1.244AsnMet: 1.244 ± 0.027
1.648AsnAsn: 1.648 ± 0.037
2.036AsnPro: 2.036 ± 0.038
1.753AsnGln: 1.753 ± 0.041
2.386AsnArg: 2.386 ± 0.044
2.172AsnSer: 2.172 ± 0.04
2.166AsnThr: 2.166 ± 0.039
2.591AsnVal: 2.591 ± 0.047
0.423AsnTrp: 0.423 ± 0.016
1.703AsnTyr: 1.703 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
2.66ProAla: 2.66 ± 0.054
0.461ProCys: 0.461 ± 0.018
2.505ProAsp: 2.505 ± 0.042
3.511ProGlu: 3.511 ± 0.059
1.574ProPhe: 1.574 ± 0.035
2.67ProGly: 2.67 ± 0.052
0.567ProHis: 0.567 ± 0.018
1.971ProIle: 1.971 ± 0.037
1.897ProLys: 1.897 ± 0.037
2.736ProLeu: 2.736 ± 0.047
0.965ProMet: 0.965 ± 0.025
1.113ProAsn: 1.113 ± 0.031
0.92ProPro: 0.92 ± 0.027
1.123ProGln: 1.123 ± 0.026
1.161ProArg: 1.161 ± 0.031
1.879ProSer: 1.879 ± 0.036
1.317ProThr: 1.317 ± 0.03
3.133ProVal: 3.133 ± 0.06
0.374ProTrp: 0.374 ± 0.016
1.459ProTyr: 1.459 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.847GlnAla: 2.847 ± 0.044
0.426GlnCys: 0.426 ± 0.017
1.906GlnAsp: 1.906 ± 0.037
3.567GlnGlu: 3.567 ± 0.053
1.203GlnPhe: 1.203 ± 0.03
2.467GlnGly: 2.467 ± 0.046
0.48GlnHis: 0.48 ± 0.017
2.841GlnIle: 2.841 ± 0.039
2.68GlnLys: 2.68 ± 0.047
2.689GlnLeu: 2.689 ± 0.045
1.322GlnMet: 1.322 ± 0.03
1.607GlnAsn: 1.607 ± 0.029
1.059GlnPro: 1.059 ± 0.033
1.349GlnGln: 1.349 ± 0.029
1.77GlnArg: 1.77 ± 0.035
1.8GlnSer: 1.8 ± 0.037
1.808GlnThr: 1.808 ± 0.038
2.647GlnVal: 2.647 ± 0.049
0.424GlnTrp: 0.424 ± 0.017
1.63GlnTyr: 1.63 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
3.1ArgAla: 3.1 ± 0.04
0.695ArgCys: 0.695 ± 0.025
2.696ArgAsp: 2.696 ± 0.043
4.675ArgGlu: 4.675 ± 0.069
2.284ArgPhe: 2.284 ± 0.035
2.894ArgGly: 2.894 ± 0.045
0.993ArgHis: 0.993 ± 0.03
4.193ArgIle: 4.193 ± 0.061
3.749ArgLys: 3.749 ± 0.054
5.225ArgLeu: 5.225 ± 0.068
1.918ArgMet: 1.918 ± 0.039
2.268ArgAsn: 2.268 ± 0.039
1.513ArgPro: 1.513 ± 0.035
2.471ArgGln: 2.471 ± 0.046
3.252ArgArg: 3.252 ± 0.055
2.591ArgSer: 2.591 ± 0.045
2.583ArgThr: 2.583 ± 0.04
3.019ArgVal: 3.019 ± 0.05
0.514ArgTrp: 0.514 ± 0.018
2.323ArgTyr: 2.323 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
4.183SerAla: 4.183 ± 0.061
0.91SerCys: 0.91 ± 0.023
3.125SerAsp: 3.125 ± 0.047
3.816SerGlu: 3.816 ± 0.055
2.745SerPhe: 2.745 ± 0.041
5.35SerGly: 5.35 ± 0.077
1.159SerHis: 1.159 ± 0.028
3.793SerIle: 3.793 ± 0.049
2.984SerLys: 2.984 ± 0.052
5.382SerLeu: 5.382 ± 0.064
1.842SerMet: 1.842 ± 0.037
2.07SerAsn: 2.07 ± 0.038
2.026SerPro: 2.026 ± 0.039
2.166SerGln: 2.166 ± 0.049
3.234SerArg: 3.234 ± 0.052
3.697SerSer: 3.697 ± 0.062
2.415SerThr: 2.415 ± 0.039
4.196SerVal: 4.196 ± 0.052
0.65SerTrp: 0.65 ± 0.022
2.531SerTyr: 2.531 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.378ThrAla: 4.378 ± 0.075
0.621ThrCys: 0.621 ± 0.019
2.898ThrAsp: 2.898 ± 0.048
3.728ThrGlu: 3.728 ± 0.06
1.974ThrPhe: 1.974 ± 0.043
4.597ThrGly: 4.597 ± 0.071
0.791ThrHis: 0.791 ± 0.021
3.447ThrIle: 3.447 ± 0.052
2.559ThrLys: 2.559 ± 0.041
4.257ThrLeu: 4.257 ± 0.06
1.402ThrMet: 1.402 ± 0.035
1.721ThrAsn: 1.721 ± 0.039
2.034ThrPro: 2.034 ± 0.037
1.394ThrGln: 1.394 ± 0.029
2.174ThrArg: 2.174 ± 0.036
2.68ThrSer: 2.68 ± 0.044
2.293ThrThr: 2.293 ± 0.044
3.941ThrVal: 3.941 ± 0.059
0.533ThrTrp: 0.533 ± 0.02
1.875ThrTyr: 1.875 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
4.239ValAla: 4.239 ± 0.054
1.23ValCys: 1.23 ± 0.03
3.43ValAsp: 3.43 ± 0.052
4.448ValGlu: 4.448 ± 0.057
3.134ValPhe: 3.134 ± 0.051
4.145ValGly: 4.145 ± 0.054
1.066ValHis: 1.066 ± 0.03
4.767ValIle: 4.767 ± 0.067
4.047ValLys: 4.047 ± 0.06
6.628ValLeu: 6.628 ± 0.08
2.249ValMet: 2.249 ± 0.038
2.639ValAsn: 2.639 ± 0.044
2.458ValPro: 2.458 ± 0.042
2.124ValGln: 2.124 ± 0.038
3.503ValArg: 3.503 ± 0.048
4.49ValSer: 4.49 ± 0.067
3.647ValThr: 3.647 ± 0.059
4.503ValVal: 4.503 ± 0.069
0.771ValTrp: 0.771 ± 0.026
2.738ValTyr: 2.738 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.583TrpAla: 0.583 ± 0.022
0.188TrpCys: 0.188 ± 0.012
0.674TrpAsp: 0.674 ± 0.023
0.852TrpGlu: 0.852 ± 0.023
0.458TrpPhe: 0.458 ± 0.017
0.753TrpGly: 0.753 ± 0.025
0.188TrpHis: 0.188 ± 0.011
0.794TrpIle: 0.794 ± 0.025
0.835TrpLys: 0.835 ± 0.026
1.071TrpLeu: 1.071 ± 0.031
0.405TrpMet: 0.405 ± 0.018
0.595TrpAsn: 0.595 ± 0.02
0.231TrpPro: 0.231 ± 0.013
0.474TrpGln: 0.474 ± 0.019
0.552TrpArg: 0.552 ± 0.02
0.51TrpSer: 0.51 ± 0.018
0.488TrpThr: 0.488 ± 0.017
0.642TrpVal: 0.642 ± 0.022
0.153TrpTrp: 0.153 ± 0.01
0.56TrpTyr: 0.56 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.607TyrAla: 2.607 ± 0.045
0.739TyrCys: 0.739 ± 0.023
2.561TyrAsp: 2.561 ± 0.054
3.214TyrGlu: 3.214 ± 0.051
1.917TyrPhe: 1.917 ± 0.039
3.345TyrGly: 3.345 ± 0.049
0.945TyrHis: 0.945 ± 0.025
2.541TyrIle: 2.541 ± 0.041
2.078TyrLys: 2.078 ± 0.039
3.982TyrLeu: 3.982 ± 0.059
1.121TyrMet: 1.121 ± 0.025
1.676TyrAsn: 1.676 ± 0.04
1.517TyrPro: 1.517 ± 0.03
1.89TyrGln: 1.89 ± 0.031
2.381TyrArg: 2.381 ± 0.039
2.344TyrSer: 2.344 ± 0.047
2.072TyrThr: 2.072 ± 0.042
2.492TyrVal: 2.492 ± 0.039
0.446TyrTrp: 0.446 ± 0.019
2.065TyrTyr: 2.065 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5047 proteins (1530207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski