Sulfodiicoccus acidiphilus
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2410 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A348B5D5|A0A348B5D5_9CREN Uncharacterized protein OS=Sulfodiicoccus acidiphilus OX=1670455 GN=HS1genome_1776 PE=4 SV=1
MM1 pKa = 8.09 AMIGGVLFYY10 pKa = 11.31 VDD12 pKa = 3.5 VVLNAPHH19 pKa = 6.47 TVQVHH24 pKa = 5.57 TSTVPPPTSGNGQPSSVSVDD44 pKa = 3.44 SLVHH48 pKa = 4.64 STYY51 pKa = 10.68 FLTPEE56 pKa = 4.1 ASLSEE61 pKa = 4.17 VDD63 pKa = 4.62 GLLAVQSSASVQGVVFPVRR82 pKa = 11.84 PSGNEE87 pKa = 3.52 LTVTLVAYY95 pKa = 10.18 VDD97 pKa = 3.92 NSTLPIAYY105 pKa = 9.84 NPGISLLTGDD115 pKa = 3.81 VSAYY119 pKa = 7.94 NTLDD123 pKa = 3.35 PQGSVWITMWGQGGGANARR142 pKa = 11.84 VFVNGNSNPLVQEE155 pKa = 4.23 WSAGGGEE162 pKa = 4.21 FTFQFVNDD170 pKa = 3.5 NGYY173 pKa = 9.96 VEE175 pKa = 4.63 LAQVVVNGAAYY186 pKa = 10.23 SPHH189 pKa = 7.88 LDD191 pKa = 3.46 TGIPWASVDD200 pKa = 3.52 GVGIVADD207 pKa = 3.76 NGVLLVQSLVVNGQVLVSS225 pKa = 3.66
Molecular weight: 23.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.85
IPC2_protein 3.884
IPC_protein 3.834
Toseland 3.63
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.541
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.961
Patrickios 0.248
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|A0A348B2P2|A0A348B2P2_9CREN Plasmid stabilization protein OS=Sulfodiicoccus acidiphilus OX=1670455 GN=HS1genome_0833 PE=4 SV=1
MM1 pKa = 8.04 DD2 pKa = 5.79 LFNSPSRR9 pKa = 11.84 KK10 pKa = 9.2 LRR12 pKa = 11.84 EE13 pKa = 3.61 IGVRR17 pKa = 11.84 GVAEE21 pKa = 4.38 ACALMAAGPGSRR33 pKa = 11.84 LLMRR37 pKa = 11.84 KK38 pKa = 9.11 IPYY41 pKa = 9.0 RR42 pKa = 11.84 GQMTVAAAAYY52 pKa = 9.2 GG53 pKa = 3.54
Molecular weight: 5.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.736
IPC_protein 10.613
Toseland 10.496
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.804
Grimsley 10.73
Solomon 10.774
Lehninger 10.745
Nozaki 10.511
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.906
Sillero 10.584
Patrickios 10.657
IPC_peptide 10.774
IPC2_peptide 9.575
IPC2.peptide.svr19 8.362
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2410
0
2410
625351
39
1376
259.5
28.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.287 ± 0.052
0.617 ± 0.016
4.551 ± 0.046
7.164 ± 0.063
3.997 ± 0.036
7.921 ± 0.043
1.437 ± 0.019
5.036 ± 0.046
5.305 ± 0.043
10.949 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.406 ± 0.023
3.09 ± 0.03
4.556 ± 0.033
2.276 ± 0.029
6.463 ± 0.062
7.084 ± 0.05
5.101 ± 0.049
9.955 ± 0.05
1.19 ± 0.02
3.616 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here