Streptococcus satellite phage Javan27

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHI8|A0A4D5ZHI8_9VIRU Bro-N domain-containing protein OS=Streptococcus satellite phage Javan27 OX=2558599 GN=JavanS27_0018 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.67TPKK9 pKa = 10.21TFTFHH14 pKa = 4.61YY15 pKa = 8.5TFRR18 pKa = 11.84DD19 pKa = 3.22FDD21 pKa = 4.07TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.77MTGTYY38 pKa = 7.11EE39 pKa = 4.76QPVIDD44 pKa = 3.97ATYY47 pKa = 10.82HH48 pKa = 6.24NDD50 pKa = 3.59DD51 pKa = 3.72QGGHH55 pKa = 6.63ANQLVLKK62 pKa = 9.62YY63 pKa = 10.97VEE65 pKa = 4.33DD66 pKa = 3.91RR67 pKa = 11.84KK68 pKa = 10.54LSKK71 pKa = 10.3VFKK74 pKa = 10.15RR75 pKa = 11.84ICDD78 pKa = 3.69SFKK81 pKa = 10.93DD82 pKa = 4.21YY83 pKa = 11.52YY84 pKa = 10.51NQPEE88 pKa = 4.31DD89 pKa = 3.62MTDD92 pKa = 3.31EE93 pKa = 4.68EE94 pKa = 5.58LDD96 pKa = 4.63DD97 pKa = 3.7MAQEE101 pKa = 4.07NEE103 pKa = 4.4LIKK106 pKa = 10.19EE107 pKa = 4.19VKK109 pKa = 8.91MCNDD113 pKa = 2.85NQLFTNEE120 pKa = 4.11YY121 pKa = 7.23IAKK124 pKa = 8.66RR125 pKa = 11.84TTQLEE130 pKa = 4.71DD131 pKa = 3.03IDD133 pKa = 4.01KK134 pKa = 10.12TILIRR139 pKa = 11.84DD140 pKa = 4.19IIAYY144 pKa = 9.18EE145 pKa = 4.47LEE147 pKa = 4.08LLDD150 pKa = 3.89YY151 pKa = 11.08ADD153 pKa = 5.25RR154 pKa = 11.84LLSDD158 pKa = 3.43KK159 pKa = 10.57SIRR162 pKa = 11.84MDD164 pKa = 3.36SEE166 pKa = 4.29TAQGTIEE173 pKa = 5.02LMDD176 pKa = 5.05DD177 pKa = 3.42NVINLVKK184 pKa = 10.53EE185 pKa = 3.94LDD187 pKa = 3.67TEE189 pKa = 4.21RR190 pKa = 11.84EE191 pKa = 4.1YY192 pKa = 11.12QGLHH196 pKa = 6.21NYY198 pKa = 9.05VISS201 pKa = 3.68

Molecular weight: 23.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZMT9|A0A4D5ZMT9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan27 OX=2558599 GN=JavanS27_0023 PE=4 SV=1
MM1 pKa = 6.48MTVAKK6 pKa = 9.8QIPNVSTLKK15 pKa = 10.86YY16 pKa = 9.99LGATAKK22 pKa = 10.23HH23 pKa = 5.49VNGVAKK29 pKa = 10.43RR30 pKa = 11.84KK31 pKa = 9.72QNFLSQISLIPTNPQLPHH49 pKa = 4.22QTIINTYY56 pKa = 10.21LYY58 pKa = 7.75WQPP61 pKa = 3.46

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

3826

41

390

136.6

15.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.94 ± 0.329

0.653 ± 0.187

5.959 ± 0.58

7.893 ± 0.528

4.705 ± 0.333

3.738 ± 0.236

1.986 ± 0.321

8.181 ± 0.498

10.037 ± 0.49

10.01 ± 0.439

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.181

6.43 ± 0.393

2.509 ± 0.322

4.391 ± 0.38

4.679 ± 0.375

5.881 ± 0.392

5.541 ± 0.451

4.548 ± 0.263

0.81 ± 0.125

4.861 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski