Amino acid dipepetide frequency for Tetrasphaera sp. HKS02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.125AlaAla: 21.125 ± 0.245	0.994AlaCys: 0.994 ± 0.034	7.919AlaAsp: 7.919 ± 0.103	7.075AlaGlu: 7.075 ± 0.106	3.474AlaPhe: 3.474 ± 0.063	12.73AlaGly: 12.73 ± 0.136	2.782AlaHis: 2.782 ± 0.063	4.449AlaIle: 4.449 ± 0.075	2.928AlaLys: 2.928 ± 0.078	14.134AlaLeu: 14.134 ± 0.161	2.875AlaMet: 2.875 ± 0.055	2.282AlaAsn: 2.282 ± 0.054	6.539AlaPro: 6.539 ± 0.106	4.445AlaGln: 4.445 ± 0.08	9.803AlaArg: 9.803 ± 0.122	7.164AlaSer: 7.164 ± 0.118	8.473AlaThr: 8.473 ± 0.123	12.31AlaVal: 12.31 ± 0.149	1.983AlaTrp: 1.983 ± 0.056	2.767AlaTyr: 2.767 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.048CysAla: 1.048 ± 0.037	0.117CysCys: 0.117 ± 0.011	0.448CysAsp: 0.448 ± 0.026	0.335CysGlu: 0.335 ± 0.017	0.199CysPhe: 0.199 ± 0.015	0.842CysGly: 0.842 ± 0.03	0.173CysHis: 0.173 ± 0.014	0.2CysIle: 0.2 ± 0.015	0.101CysLys: 0.101 ± 0.01	0.642CysLeu: 0.642 ± 0.027	0.107CysMet: 0.107 ± 0.01	0.126CysAsn: 0.126 ± 0.013	0.499CysPro: 0.499 ± 0.027	0.187CysGln: 0.187 ± 0.013	0.576CysArg: 0.576 ± 0.024	0.475CysSer: 0.475 ± 0.025	0.509CysThr: 0.509 ± 0.027	0.645CysVal: 0.645 ± 0.032	0.098CysTrp: 0.098 ± 0.01	0.123CysTyr: 0.123 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.655AspAla: 7.655 ± 0.104	0.369AspCys: 0.369 ± 0.018	3.579AspAsp: 3.579 ± 0.069	3.745AspGlu: 3.745 ± 0.068	1.554AspPhe: 1.554 ± 0.043	5.892AspGly: 5.892 ± 0.086	1.584AspHis: 1.584 ± 0.045	1.952AspIle: 1.952 ± 0.045	1.094AspLys: 1.094 ± 0.044	6.805AspLeu: 6.805 ± 0.103	0.719AspMet: 0.719 ± 0.026	0.954AspAsn: 0.954 ± 0.035	4.285AspPro: 4.285 ± 0.069	1.896AspGln: 1.896 ± 0.045	4.559AspArg: 4.559 ± 0.078	2.583AspSer: 2.583 ± 0.053	2.956AspThr: 2.956 ± 0.056	5.435AspVal: 5.435 ± 0.084	0.89AspTrp: 0.89 ± 0.033	1.112AspTyr: 1.112 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	6.64GluAla: 6.64 ± 0.107	0.326GluCys: 0.326 ± 0.019	2.556GluAsp: 2.556 ± 0.051	2.501GluGlu: 2.501 ± 0.063	1.462GluPhe: 1.462 ± 0.045	3.898GluGly: 3.898 ± 0.069	1.508GluHis: 1.508 ± 0.043	2.02GluIle: 2.02 ± 0.051	1.018GluLys: 1.018 ± 0.045	6.007GluLeu: 6.007 ± 0.097	0.814GluMet: 0.814 ± 0.03	0.817GluAsn: 0.817 ± 0.029	2.891GluPro: 2.891 ± 0.06	2.11GluGln: 2.11 ± 0.056	4.822GluArg: 4.822 ± 0.087	2.582GluSer: 2.582 ± 0.055	2.456GluThr: 2.456 ± 0.056	5.201GluVal: 5.201 ± 0.084	0.635GluTrp: 0.635 ± 0.025	0.869GluTyr: 0.869 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.67PheAla: 3.67 ± 0.073	0.217PheCys: 0.217 ± 0.016	2.006PheAsp: 2.006 ± 0.051	1.497PheGlu: 1.497 ± 0.042	0.854PhePhe: 0.854 ± 0.033	2.962PheGly: 2.962 ± 0.059	0.583PheHis: 0.583 ± 0.026	0.859PheIle: 0.859 ± 0.033	0.474PheLys: 0.474 ± 0.023	2.432PheLeu: 2.432 ± 0.062	0.428PheMet: 0.428 ± 0.022	0.602PheAsn: 0.602 ± 0.029	1.271PhePro: 1.271 ± 0.037	0.646PheGln: 0.646 ± 0.026	1.586PheArg: 1.586 ± 0.04	1.562PheSer: 1.562 ± 0.044	1.885PheThr: 1.885 ± 0.045	2.593PheVal: 2.593 ± 0.059	0.41PheTrp: 0.41 ± 0.021	0.585PheTyr: 0.585 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	11.134GlyAla: 11.134 ± 0.118	0.785GlyCys: 0.785 ± 0.031	5.031GlyAsp: 5.031 ± 0.079	4.473GlyGlu: 4.473 ± 0.074	2.85GlyPhe: 2.85 ± 0.054	8.254GlyGly: 8.254 ± 0.12	2.324GlyHis: 2.324 ± 0.053	3.773GlyIle: 3.773 ± 0.068	2.193GlyLys: 2.193 ± 0.065	9.732GlyLeu: 9.732 ± 0.123	2.003GlyMet: 2.003 ± 0.045	1.68GlyAsn: 1.68 ± 0.053	4.726GlyPro: 4.726 ± 0.071	3.025GlyGln: 3.025 ± 0.055	6.994GlyArg: 6.994 ± 0.098	5.885GlySer: 5.885 ± 0.106	5.836GlyThr: 5.836 ± 0.117	8.251GlyVal: 8.251 ± 0.084	1.763GlyTrp: 1.763 ± 0.041	2.157GlyTyr: 2.157 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.718HisAla: 2.718 ± 0.055	0.201HisCys: 0.201 ± 0.015	1.509HisAsp: 1.509 ± 0.039	1.32HisGlu: 1.32 ± 0.042	0.548HisPhe: 0.548 ± 0.022	2.354HisGly: 2.354 ± 0.058	0.731HisHis: 0.731 ± 0.032	0.627HisIle: 0.627 ± 0.027	0.304HisLys: 0.304 ± 0.018	2.468HisLeu: 2.468 ± 0.055	0.295HisMet: 0.295 ± 0.018	0.358HisAsn: 0.358 ± 0.021	1.701HisPro: 1.701 ± 0.044	0.703HisGln: 0.703 ± 0.025	1.902HisArg: 1.902 ± 0.058	0.992HisSer: 0.992 ± 0.032	1.314HisThr: 1.314 ± 0.046	1.946HisVal: 1.946 ± 0.053	0.35HisTrp: 0.35 ± 0.02	0.437HisTyr: 0.437 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	4.937IleAla: 4.937 ± 0.081	0.303IleCys: 0.303 ± 0.018	2.602IleAsp: 2.602 ± 0.052	2.106IleGlu: 2.106 ± 0.056	0.82IlePhe: 0.82 ± 0.036	3.568IleGly: 3.568 ± 0.072	0.715IleHis: 0.715 ± 0.027	1.108IleIle: 1.108 ± 0.041	0.719IleLys: 0.719 ± 0.031	2.706IleLeu: 2.706 ± 0.061	0.461IleMet: 0.461 ± 0.023	0.741IleAsn: 0.741 ± 0.027	1.928IlePro: 1.928 ± 0.051	0.795IleGln: 0.795 ± 0.032	2.218IleArg: 2.218 ± 0.057	1.893IleSer: 1.893 ± 0.044	2.39IleThr: 2.39 ± 0.058	3.162IleVal: 3.162 ± 0.064	0.379IleTrp: 0.379 ± 0.021	0.598IleTyr: 0.598 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	2.903LysAla: 2.903 ± 0.074	0.096LysCys: 0.096 ± 0.012	1.264LysAsp: 1.264 ± 0.044	0.923LysGlu: 0.923 ± 0.04	0.475LysPhe: 0.475 ± 0.023	1.759LysGly: 1.759 ± 0.048	0.38LysHis: 0.38 ± 0.021	0.733LysIle: 0.733 ± 0.028	0.633LysLys: 0.633 ± 0.039	1.631LysLeu: 1.631 ± 0.047	0.332LysMet: 0.332 ± 0.017	0.471LysAsn: 0.471 ± 0.022	1.178LysPro: 1.178 ± 0.043	0.63LysGln: 0.63 ± 0.025	1.3LysArg: 1.3 ± 0.042	1.183LysSer: 1.183 ± 0.042	1.301LysThr: 1.301 ± 0.051	1.895LysVal: 1.895 ± 0.054	0.219LysTrp: 0.219 ± 0.015	0.419LysTyr: 0.419 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	15.168LeuAla: 15.168 ± 0.156	0.74LeuCys: 0.74 ± 0.033	6.649LeuAsp: 6.649 ± 0.102	5.102LeuGlu: 5.102 ± 0.089	2.349LeuPhe: 2.349 ± 0.066	9.779LeuGly: 9.779 ± 0.103	2.077LeuHis: 2.077 ± 0.052	2.94LeuIle: 2.94 ± 0.069	1.794LeuLys: 1.794 ± 0.052	10.063LeuLeu: 10.063 ± 0.15	1.673LeuMet: 1.673 ± 0.046	1.596LeuAsn: 1.596 ± 0.04	5.751LeuPro: 5.751 ± 0.08	2.462LeuGln: 2.462 ± 0.055	8.037LeuArg: 8.037 ± 0.115	5.539LeuSer: 5.539 ± 0.08	6.577LeuThr: 6.577 ± 0.086	10.325LeuVal: 10.325 ± 0.136	1.228LeuTrp: 1.228 ± 0.035	1.508LeuTyr: 1.508 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.518MetAla: 2.518 ± 0.054	0.134MetCys: 0.134 ± 0.014	0.886MetAsp: 0.886 ± 0.034	0.679MetGlu: 0.679 ± 0.031	0.474MetPhe: 0.474 ± 0.023	1.413MetGly: 1.413 ± 0.037	0.34MetHis: 0.34 ± 0.02	0.607MetIle: 0.607 ± 0.026	0.406MetLys: 0.406 ± 0.022	1.749MetLeu: 1.749 ± 0.05	0.302MetMet: 0.302 ± 0.017	0.383MetAsn: 0.383 ± 0.021	1.205MetPro: 1.205 ± 0.033	0.515MetGln: 0.515 ± 0.023	1.387MetArg: 1.387 ± 0.04	1.448MetSer: 1.448 ± 0.042	1.635MetThr: 1.635 ± 0.04	1.625MetVal: 1.625 ± 0.047	0.196MetTrp: 0.196 ± 0.014	0.285MetTyr: 0.285 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.325AsnAla: 2.325 ± 0.048	0.138AsnCys: 0.138 ± 0.012	0.991AsnAsp: 0.991 ± 0.036	0.739AsnGlu: 0.739 ± 0.026	0.479AsnPhe: 0.479 ± 0.022	1.752AsnGly: 1.752 ± 0.063	0.429AsnHis: 0.429 ± 0.021	0.632AsnIle: 0.632 ± 0.027	0.334AsnLys: 0.334 ± 0.021	1.982AsnLeu: 1.982 ± 0.052	0.226AsnMet: 0.226 ± 0.017	0.446AsnAsn: 0.446 ± 0.027	1.476AsnPro: 1.476 ± 0.042	0.544AsnGln: 0.544 ± 0.027	1.208AsnArg: 1.208 ± 0.039	0.91AsnSer: 0.91 ± 0.034	1.062AsnThr: 1.062 ± 0.043	1.494AsnVal: 1.494 ± 0.038	0.274AsnTrp: 0.274 ± 0.018	0.377AsnTyr: 0.377 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.671ProAla: 7.671 ± 0.109	0.353ProCys: 0.353 ± 0.02	4.019ProAsp: 4.019 ± 0.066	3.323ProGlu: 3.323 ± 0.067	1.569ProPhe: 1.569 ± 0.042	6.01ProGly: 6.01 ± 0.087	1.294ProHis: 1.294 ± 0.035	1.589ProIle: 1.589 ± 0.048	1.08ProLys: 1.08 ± 0.039	4.83ProLeu: 4.83 ± 0.063	1.088ProMet: 1.088 ± 0.036	0.954ProAsn: 0.954 ± 0.032	3.014ProPro: 3.014 ± 0.085	1.917ProGln: 1.917 ± 0.053	3.888ProArg: 3.888 ± 0.066	3.534ProSer: 3.534 ± 0.07	3.915ProThr: 3.915 ± 0.079	5.368ProVal: 5.368 ± 0.079	0.959ProTrp: 0.959 ± 0.031	1.037ProTyr: 1.037 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.102GlnAla: 4.102 ± 0.075	0.185GlnCys: 0.185 ± 0.015	1.442GlnAsp: 1.442 ± 0.038	1.318GlnGlu: 1.318 ± 0.04	0.82GlnPhe: 0.82 ± 0.032	2.451GlnGly: 2.451 ± 0.056	0.706GlnHis: 0.706 ± 0.025	1.194GlnIle: 1.194 ± 0.036	0.6GlnLys: 0.6 ± 0.027	3.364GlnLeu: 3.364 ± 0.067	0.56GlnMet: 0.56 ± 0.024	0.534GlnAsn: 0.534 ± 0.026	1.814GlnPro: 1.814 ± 0.051	1.267GlnGln: 1.267 ± 0.043	2.682GlnArg: 2.682 ± 0.059	1.506GlnSer: 1.506 ± 0.041	1.687GlnThr: 1.687 ± 0.041	3.036GlnVal: 3.036 ± 0.051	0.532GlnTrp: 0.532 ± 0.024	0.567GlnTyr: 0.567 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	9.395ArgAla: 9.395 ± 0.124	0.527ArgCys: 0.527 ± 0.025	4.373ArgAsp: 4.373 ± 0.079	4.177ArgGlu: 4.177 ± 0.074	2.149ArgPhe: 2.149 ± 0.051	5.872ArgGly: 5.872 ± 0.09	1.987ArgHis: 1.987 ± 0.049	2.896ArgIle: 2.896 ± 0.063	1.272ArgLys: 1.272 ± 0.038	7.926ArgLeu: 7.926 ± 0.099	1.718ArgMet: 1.718 ± 0.047	1.145ArgAsn: 1.145 ± 0.038	4.52ArgPro: 4.52 ± 0.082	2.356ArgGln: 2.356 ± 0.052	7.147ArgArg: 7.147 ± 0.128	4.256ArgSer: 4.256 ± 0.081	4.517ArgThr: 4.517 ± 0.073	6.331ArgVal: 6.331 ± 0.083	1.384ArgTrp: 1.384 ± 0.04	1.584ArgTyr: 1.584 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	7.512SerAla: 7.512 ± 0.134	0.43SerCys: 0.43 ± 0.024	2.949SerAsp: 2.949 ± 0.058	2.367SerGlu: 2.367 ± 0.044	1.623SerPhe: 1.623 ± 0.041	6.091SerGly: 6.091 ± 0.098	1.141SerHis: 1.141 ± 0.038	1.883SerIle: 1.883 ± 0.05	1.014SerLys: 1.014 ± 0.034	5.31SerLeu: 5.31 ± 0.065	1.309SerMet: 1.309 ± 0.042	0.973SerAsn: 0.973 ± 0.035	3.492SerPro: 3.492 ± 0.064	1.596SerGln: 1.596 ± 0.04	3.899SerArg: 3.899 ± 0.072	3.669SerSer: 3.669 ± 0.073	3.908SerThr: 3.908 ± 0.078	4.942SerVal: 4.942 ± 0.075	0.977SerTrp: 0.977 ± 0.033	1.114SerTyr: 1.114 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	8.236ThrAla: 8.236 ± 0.141	0.484ThrCys: 0.484 ± 0.034	3.449ThrAsp: 3.449 ± 0.071	2.795ThrGlu: 2.795 ± 0.06	1.839ThrPhe: 1.839 ± 0.05	6.212ThrGly: 6.212 ± 0.102	1.28ThrHis: 1.28 ± 0.036	2.356ThrIle: 2.356 ± 0.053	1.354ThrLys: 1.354 ± 0.051	5.94ThrLeu: 5.94 ± 0.088	1.15ThrMet: 1.15 ± 0.037	1.23ThrAsn: 1.23 ± 0.056	4.197ThrPro: 4.197 ± 0.109	1.695ThrGln: 1.695 ± 0.051	4.05ThrArg: 4.05 ± 0.073	3.892ThrSer: 3.892 ± 0.073	4.93ThrThr: 4.93 ± 0.13	6.288ThrVal: 6.288 ± 0.095	0.989ThrTrp: 0.989 ± 0.036	1.296ThrTyr: 1.296 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	12.803ValAla: 12.803 ± 0.14	0.738ValCys: 0.738 ± 0.027	5.815ValAsp: 5.815 ± 0.079	5.124ValGlu: 5.124 ± 0.083	2.508ValPhe: 2.508 ± 0.056	8.074ValGly: 8.074 ± 0.113	1.988ValHis: 1.988 ± 0.049	3.346ValIle: 3.346 ± 0.066	1.8ValLys: 1.8 ± 0.05	9.79ValLeu: 9.79 ± 0.117	1.647ValMet: 1.647 ± 0.048	1.772ValAsn: 1.772 ± 0.045	5.09ValPro: 5.09 ± 0.081	2.446ValGln: 2.446 ± 0.046	6.734ValArg: 6.734 ± 0.087	5.112ValSer: 5.112 ± 0.088	6.278ValThr: 6.278 ± 0.119	10.442ValVal: 10.442 ± 0.149	1.174ValTrp: 1.174 ± 0.035	1.506ValTyr: 1.506 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.717TrpAla: 1.717 ± 0.048	0.16TrpCys: 0.16 ± 0.014	0.745TrpAsp: 0.745 ± 0.027	0.595TrpGlu: 0.595 ± 0.025	0.494TrpPhe: 0.494 ± 0.024	1.141TrpGly: 1.141 ± 0.035	0.398TrpHis: 0.398 ± 0.021	0.482TrpIle: 0.482 ± 0.025	0.294TrpLys: 0.294 ± 0.019	1.752TrpLeu: 1.752 ± 0.053	0.307TrpMet: 0.307 ± 0.018	0.368TrpAsn: 0.368 ± 0.019	0.87TrpPro: 0.87 ± 0.035	0.553TrpGln: 0.553 ± 0.025	1.303TrpArg: 1.303 ± 0.044	1.027TrpSer: 1.027 ± 0.033	0.957TrpThr: 0.957 ± 0.039	1.282TrpVal: 1.282 ± 0.038	0.373TrpTrp: 0.373 ± 0.021	0.287TrpTyr: 0.287 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.974TyrAla: 2.974 ± 0.059	0.156TyrCys: 0.156 ± 0.014	1.389TyrAsp: 1.389 ± 0.04	0.94TyrGlu: 0.94 ± 0.033	0.583TyrPhe: 0.583 ± 0.027	1.815TyrGly: 1.815 ± 0.05	0.354TyrHis: 0.354 ± 0.019	0.503TyrIle: 0.503 ± 0.021	0.313TyrLys: 0.313 ± 0.02	2.091TyrLeu: 2.091 ± 0.047	0.173TyrMet: 0.173 ± 0.015	0.386TyrAsn: 0.386 ± 0.023	0.965TyrPro: 0.965 ± 0.033	0.581TyrGln: 0.581 ± 0.029	1.439TyrArg: 1.439 ± 0.041	0.986TyrSer: 0.986 ± 0.032	1.076TyrThr: 1.076 ± 0.043	1.613TyrVal: 1.613 ± 0.045	0.289TyrTrp: 0.289 ± 0.02	0.451TyrTyr: 0.451 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3054 proteins (957057 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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