Amino acid dipepetide frequency for Anoxybacillus flavithermus (strain DSM 21510 / WK1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.499AlaAla: 5.499 ± 0.119
0.755AlaCys: 0.755 ± 0.03
3.228AlaAsp: 3.228 ± 0.06
4.598AlaGlu: 4.598 ± 0.079
3.385AlaPhe: 3.385 ± 0.08
4.771AlaGly: 4.771 ± 0.092
1.87AlaHis: 1.87 ± 0.05
6.2AlaIle: 6.2 ± 0.099
5.535AlaLys: 5.535 ± 0.091
8.073AlaLeu: 8.073 ± 0.109
2.385AlaMet: 2.385 ± 0.056
2.828AlaAsn: 2.828 ± 0.066
2.277AlaPro: 2.277 ± 0.06
2.748AlaGln: 2.748 ± 0.06
3.394AlaArg: 3.394 ± 0.062
3.743AlaSer: 3.743 ± 0.068
4.185AlaThr: 4.185 ± 0.072
5.61AlaVal: 5.61 ± 0.09
0.719AlaTrp: 0.719 ± 0.034
2.755AlaTyr: 2.755 ± 0.06
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.03
0.154CysCys: 0.154 ± 0.015
0.465CysAsp: 0.465 ± 0.024
0.647CysGlu: 0.647 ± 0.03
0.389CysPhe: 0.389 ± 0.025
0.762CysGly: 0.762 ± 0.03
0.277CysHis: 0.277 ± 0.02
0.604CysIle: 0.604 ± 0.029
0.38CysLys: 0.38 ± 0.02
0.769CysLeu: 0.769 ± 0.033
0.266CysMet: 0.266 ± 0.017
0.309CysAsn: 0.309 ± 0.019
0.437CysPro: 0.437 ± 0.024
0.289CysGln: 0.289 ± 0.021
0.423CysArg: 0.423 ± 0.024
0.505CysSer: 0.505 ± 0.023
0.511CysThr: 0.511 ± 0.025
0.582CysVal: 0.582 ± 0.027
0.069CysTrp: 0.069 ± 0.01
0.329CysTyr: 0.329 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.457AspAla: 3.457 ± 0.059
0.391AspCys: 0.391 ± 0.023
2.613AspAsp: 2.613 ± 0.069
4.907AspGlu: 4.907 ± 0.086
1.912AspPhe: 1.912 ± 0.049
3.462AspGly: 3.462 ± 0.082
1.158AspHis: 1.158 ± 0.032
3.951AspIle: 3.951 ± 0.069
2.063AspLys: 2.063 ± 0.053
4.106AspLeu: 4.106 ± 0.072
1.361AspMet: 1.361 ± 0.038
1.173AspAsn: 1.173 ± 0.037
1.925AspPro: 1.925 ± 0.047
1.542AspGln: 1.542 ± 0.04
2.675AspArg: 2.675 ± 0.057
1.623AspSer: 1.623 ± 0.048
2.031AspThr: 2.031 ± 0.051
4.94AspVal: 4.94 ± 0.079
0.571AspTrp: 0.571 ± 0.029
1.887AspTyr: 1.887 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
6.19GluAla: 6.19 ± 0.105
0.39GluCys: 0.39 ± 0.021
2.856GluAsp: 2.856 ± 0.063
6.547GluGlu: 6.547 ± 0.108
2.063GluPhe: 2.063 ± 0.052
4.258GluGly: 4.258 ± 0.075
2.11GluHis: 2.11 ± 0.059
4.358GluIle: 4.358 ± 0.073
6.526GluLys: 6.526 ± 0.098
6.527GluLeu: 6.527 ± 0.102
2.385GluMet: 2.385 ± 0.056
2.718GluAsn: 2.718 ± 0.061
1.949GluPro: 1.949 ± 0.053
5.605GluGln: 5.605 ± 0.126
5.18GluArg: 5.18 ± 0.094
2.466GluSer: 2.466 ± 0.053
4.156GluThr: 4.156 ± 0.067
4.873GluVal: 4.873 ± 0.078
0.962GluTrp: 0.962 ± 0.039
2.095GluTyr: 2.095 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.486PheAla: 3.486 ± 0.07
0.425PheCys: 0.425 ± 0.022
2.188PheAsp: 2.188 ± 0.056
2.66PheGlu: 2.66 ± 0.053
2.615PhePhe: 2.615 ± 0.058
3.135PheGly: 3.135 ± 0.068
1.334PheHis: 1.334 ± 0.041
3.737PheIle: 3.737 ± 0.08
1.695PheLys: 1.695 ± 0.054
4.565PheLeu: 4.565 ± 0.095
1.226PheMet: 1.226 ± 0.04
1.259PheAsn: 1.259 ± 0.046
1.818PhePro: 1.818 ± 0.044
1.693PheGln: 1.693 ± 0.048
1.96PheArg: 1.96 ± 0.051
3.005PheSer: 3.005 ± 0.059
2.556PheThr: 2.556 ± 0.057
3.712PheVal: 3.712 ± 0.064
0.426PheTrp: 0.426 ± 0.025
1.683PheTyr: 1.683 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
4.963GlyAla: 4.963 ± 0.091
0.666GlyCys: 0.666 ± 0.032
3.074GlyAsp: 3.074 ± 0.071
4.462GlyGlu: 4.462 ± 0.082
2.864GlyPhe: 2.864 ± 0.068
4.683GlyGly: 4.683 ± 0.103
1.492GlyHis: 1.492 ± 0.04
5.125GlyIle: 5.125 ± 0.092
5.062GlyLys: 5.062 ± 0.079
5.707GlyLeu: 5.707 ± 0.083
2.189GlyMet: 2.189 ± 0.054
2.211GlyAsn: 2.211 ± 0.054
1.548GlyPro: 1.548 ± 0.049
2.091GlyGln: 2.091 ± 0.049
2.945GlyArg: 2.945 ± 0.065
3.019GlySer: 3.019 ± 0.07
3.982GlyThr: 3.982 ± 0.088
5.333GlyVal: 5.333 ± 0.087
0.802GlyTrp: 0.802 ± 0.034
2.615GlyTyr: 2.615 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.831HisAla: 1.831 ± 0.045
0.285HisCys: 0.285 ± 0.018
1.311HisAsp: 1.311 ± 0.038
1.913HisGlu: 1.913 ± 0.046
1.188HisPhe: 1.188 ± 0.039
1.604HisGly: 1.604 ± 0.044
0.907HisHis: 0.907 ± 0.041
2.324HisIle: 2.324 ± 0.058
0.973HisLys: 0.973 ± 0.038
2.453HisLeu: 2.453 ± 0.063
0.94HisMet: 0.94 ± 0.032
0.65HisAsn: 0.65 ± 0.027
1.338HisPro: 1.338 ± 0.038
0.848HisGln: 0.848 ± 0.033
1.281HisArg: 1.281 ± 0.04
1.078HisSer: 1.078 ± 0.038
1.338HisThr: 1.338 ± 0.038
2.454HisVal: 2.454 ± 0.064
0.266HisTrp: 0.266 ± 0.02
0.972HisTyr: 0.972 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
6.606IleAla: 6.606 ± 0.106
0.731IleCys: 0.731 ± 0.032
4.527IleAsp: 4.527 ± 0.078
6.253IleGlu: 6.253 ± 0.095
3.135IlePhe: 3.135 ± 0.075
5.749IleGly: 5.749 ± 0.117
1.908IleHis: 1.908 ± 0.052
4.905IleIle: 4.905 ± 0.091
3.349IleLys: 3.349 ± 0.071
5.522IleLeu: 5.522 ± 0.102
1.47IleMet: 1.47 ± 0.041
2.24IleAsn: 2.24 ± 0.056
3.175IlePro: 3.175 ± 0.063
3.052IleGln: 3.052 ± 0.066
3.696IleArg: 3.696 ± 0.071
4.005IleSer: 4.005 ± 0.079
3.523IleThr: 3.523 ± 0.071
6.792IleVal: 6.792 ± 0.089
0.669IleTrp: 0.669 ± 0.029
2.386IleTyr: 2.386 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
4.484LysAla: 4.484 ± 0.083
0.39LysCys: 0.39 ± 0.021
2.877LysAsp: 2.877 ± 0.069
6.461LysGlu: 6.461 ± 0.103
1.575LysPhe: 1.575 ± 0.044
4.41LysGly: 4.41 ± 0.081
1.619LysHis: 1.619 ± 0.045
3.76LysIle: 3.76 ± 0.067
5.983LysLys: 5.983 ± 0.095
5.1LysLeu: 5.1 ± 0.086
2.226LysMet: 2.226 ± 0.053
2.571LysAsn: 2.571 ± 0.061
2.108LysPro: 2.108 ± 0.062
4.731LysGln: 4.731 ± 0.092
4.312LysArg: 4.312 ± 0.071
2.483LysSer: 2.483 ± 0.049
3.709LysThr: 3.709 ± 0.071
4.274LysVal: 4.274 ± 0.079
0.904LysTrp: 0.904 ± 0.036
1.961LysTyr: 1.961 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
7.851LeuAla: 7.851 ± 0.098
0.821LeuCys: 0.821 ± 0.034
4.233LeuAsp: 4.233 ± 0.083
5.759LeuGlu: 5.759 ± 0.091
5.073LeuPhe: 5.073 ± 0.106
5.631LeuGly: 5.631 ± 0.089
2.595LeuHis: 2.595 ± 0.059
5.997LeuIle: 5.997 ± 0.094
5.963LeuLys: 5.963 ± 0.09
9.932LeuLeu: 9.932 ± 0.147
2.301LeuMet: 2.301 ± 0.056
3.282LeuAsn: 3.282 ± 0.06
3.922LeuPro: 3.922 ± 0.067
4.525LeuGln: 4.525 ± 0.087
4.59LeuArg: 4.59 ± 0.084
5.981LeuSer: 5.981 ± 0.088
5.306LeuThr: 5.306 ± 0.075
6.388LeuVal: 6.388 ± 0.097
0.796LeuTrp: 0.796 ± 0.031
3.314LeuTyr: 3.314 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.172MetAla: 2.172 ± 0.059
0.235MetCys: 0.235 ± 0.017
1.269MetAsp: 1.269 ± 0.041
2.013MetGlu: 2.013 ± 0.05
1.271MetPhe: 1.271 ± 0.037
1.556MetGly: 1.556 ± 0.041
0.657MetHis: 0.657 ± 0.029
2.385MetIle: 2.385 ± 0.059
2.78MetLys: 2.78 ± 0.06
2.725MetLeu: 2.725 ± 0.061
1.234MetMet: 1.234 ± 0.047
1.686MetAsn: 1.686 ± 0.038
1.085MetPro: 1.085 ± 0.049
1.082MetGln: 1.082 ± 0.039
1.522MetArg: 1.522 ± 0.042
1.535MetSer: 1.535 ± 0.042
1.919MetThr: 1.919 ± 0.053
1.618MetVal: 1.618 ± 0.04
0.246MetTrp: 0.246 ± 0.017
1.008MetTyr: 1.008 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
2.396AsnAla: 2.396 ± 0.056
0.279AsnCys: 0.279 ± 0.017
2.166AsnAsp: 2.166 ± 0.06
3.675AsnGlu: 3.675 ± 0.068
1.175AsnPhe: 1.175 ± 0.039
2.992AsnGly: 2.992 ± 0.076
0.814AsnHis: 0.814 ± 0.033
2.992AsnIle: 2.992 ± 0.074
2.29AsnLys: 2.29 ± 0.058
2.35AsnLeu: 2.35 ± 0.055
1.223AsnMet: 1.223 ± 0.037
1.344AsnAsn: 1.344 ± 0.05
1.625AsnPro: 1.625 ± 0.046
1.187AsnGln: 1.187 ± 0.039
1.907AsnArg: 1.907 ± 0.051
1.349AsnSer: 1.349 ± 0.039
1.521AsnThr: 1.521 ± 0.046
3.216AsnVal: 3.216 ± 0.063
0.367AsnTrp: 0.367 ± 0.019
1.169AsnTyr: 1.169 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
2.112ProAla: 2.112 ± 0.053
0.27ProCys: 0.27 ± 0.018
1.718ProAsp: 1.718 ± 0.044
2.379ProGlu: 2.379 ± 0.057
2.232ProPhe: 2.232 ± 0.058
1.89ProGly: 1.89 ± 0.057
1.138ProHis: 1.138 ± 0.046
2.985ProIle: 2.985 ± 0.062
2.376ProLys: 2.376 ± 0.059
3.794ProLeu: 3.794 ± 0.072
0.911ProMet: 0.911 ± 0.039
1.884ProAsn: 1.884 ± 0.052
1.172ProPro: 1.172 ± 0.043
1.126ProGln: 1.126 ± 0.042
1.287ProArg: 1.287 ± 0.045
2.324ProSer: 2.324 ± 0.053
2.277ProThr: 2.277 ± 0.056
2.502ProVal: 2.502 ± 0.065
0.388ProTrp: 0.388 ± 0.021
1.641ProTyr: 1.641 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
3.468GlnAla: 3.468 ± 0.063
0.318GlnCys: 0.318 ± 0.019
1.284GlnAsp: 1.284 ± 0.039
2.616GlnGlu: 2.616 ± 0.067
1.978GlnPhe: 1.978 ± 0.049
2.112GlnGly: 2.112 ± 0.047
1.285GlnHis: 1.285 ± 0.047
2.696GlnIle: 2.696 ± 0.059
3.016GlnLys: 3.016 ± 0.063
5.558GlnLeu: 5.558 ± 0.099
1.542GlnMet: 1.542 ± 0.047
1.234GlnAsn: 1.234 ± 0.046
1.412GlnPro: 1.412 ± 0.048
3.172GlnGln: 3.172 ± 0.074
2.075GlnArg: 2.075 ± 0.054
2.063GlnSer: 2.063 ± 0.056
2.807GlnThr: 2.807 ± 0.062
2.498GlnVal: 2.498 ± 0.062
0.637GlnTrp: 0.637 ± 0.031
1.652GlnTyr: 1.652 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
3.432ArgAla: 3.432 ± 0.067
0.435ArgCys: 0.435 ± 0.022
2.421ArgAsp: 2.421 ± 0.059
3.811ArgGlu: 3.811 ± 0.075
2.386ArgPhe: 2.386 ± 0.054
2.897ArgGly: 2.897 ± 0.057
1.257ArgHis: 1.257 ± 0.042
3.617ArgIle: 3.617 ± 0.061
3.645ArgLys: 3.645 ± 0.075
5.112ArgLeu: 5.112 ± 0.084
1.801ArgMet: 1.801 ± 0.048
1.74ArgAsn: 1.74 ± 0.044
1.691ArgPro: 1.691 ± 0.055
2.202ArgGln: 2.202 ± 0.056
2.561ArgArg: 2.561 ± 0.062
2.413ArgSer: 2.413 ± 0.053
2.634ArgThr: 2.634 ± 0.056
3.441ArgVal: 3.441 ± 0.061
0.644ArgTrp: 0.644 ± 0.031
2.057ArgTyr: 2.057 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
3.153SerAla: 3.153 ± 0.065
0.455SerCys: 0.455 ± 0.021
2.291SerAsp: 2.291 ± 0.049
3.172SerGlu: 3.172 ± 0.066
3.509SerPhe: 3.509 ± 0.071
3.344SerGly: 3.344 ± 0.06
1.145SerHis: 1.145 ± 0.035
3.804SerIle: 3.804 ± 0.076
2.761SerLys: 2.761 ± 0.06
5.73SerLeu: 5.73 ± 0.079
1.369SerMet: 1.369 ± 0.04
1.688SerAsn: 1.688 ± 0.047
1.989SerPro: 1.989 ± 0.053
1.571SerGln: 1.571 ± 0.05
2.112SerArg: 2.112 ± 0.062
2.931SerSer: 2.931 ± 0.063
2.443SerThr: 2.443 ± 0.058
3.76SerVal: 3.76 ± 0.075
0.555SerTrp: 0.555 ± 0.025
2.007SerTyr: 2.007 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
3.594ThrAla: 3.594 ± 0.072
0.488ThrCys: 0.488 ± 0.026
2.453ThrAsp: 2.453 ± 0.055
3.245ThrGlu: 3.245 ± 0.068
3.034ThrPhe: 3.034 ± 0.062
3.653ThrGly: 3.653 ± 0.073
1.222ThrHis: 1.222 ± 0.033
4.907ThrIle: 4.907 ± 0.075
3.768ThrLys: 3.768 ± 0.076
5.288ThrLeu: 5.288 ± 0.085
1.672ThrMet: 1.672 ± 0.047
2.6ThrAsn: 2.6 ± 0.055
2.264ThrPro: 2.264 ± 0.06
1.296ThrGln: 1.296 ± 0.046
1.947ThrArg: 1.947 ± 0.051
2.777ThrSer: 2.777 ± 0.064
3.109ThrThr: 3.109 ± 0.075
4.572ThrVal: 4.572 ± 0.083
0.601ThrTrp: 0.601 ± 0.027
2.456ThrTyr: 2.456 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
6.006ValAla: 6.006 ± 0.092
0.818ValCys: 0.818 ± 0.036
3.947ValAsp: 3.947 ± 0.069
5.17ValGlu: 5.17 ± 0.087
3.163ValPhe: 3.163 ± 0.064
4.746ValGly: 4.746 ± 0.087
1.998ValHis: 1.998 ± 0.053
5.836ValIle: 5.836 ± 0.087
4.935ValLys: 4.935 ± 0.082
6.875ValLeu: 6.875 ± 0.091
2.026ValMet: 2.026 ± 0.053
2.827ValAsn: 2.827 ± 0.059
3.055ValPro: 3.055 ± 0.063
3.096ValGln: 3.096 ± 0.061
3.76ValArg: 3.76 ± 0.072
4.307ValSer: 4.307 ± 0.078
4.324ValThr: 4.324 ± 0.082
6.347ValVal: 6.347 ± 0.111
0.759ValTrp: 0.759 ± 0.036
2.707ValTyr: 2.707 ± 0.065
0.0ValXaa: 0.0 ± 0.0
Trp
0.663TrpAla: 0.663 ± 0.027
0.089TrpCys: 0.089 ± 0.011
0.48TrpAsp: 0.48 ± 0.026
0.718TrpGlu: 0.718 ± 0.033
0.498TrpPhe: 0.498 ± 0.025
0.623TrpGly: 0.623 ± 0.027
0.314TrpHis: 0.314 ± 0.019
0.893TrpIle: 0.893 ± 0.037
0.842TrpLys: 0.842 ± 0.031
1.356TrpLeu: 1.356 ± 0.036
0.401TrpMet: 0.401 ± 0.021
0.518TrpAsn: 0.518 ± 0.029
0.269TrpPro: 0.269 ± 0.021
0.397TrpGln: 0.397 ± 0.024
0.541TrpArg: 0.541 ± 0.026
0.515TrpSer: 0.515 ± 0.026
0.659TrpThr: 0.659 ± 0.027
0.626TrpVal: 0.626 ± 0.027
0.155TrpTrp: 0.155 ± 0.014
0.332TrpTyr: 0.332 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.608TyrAla: 2.608 ± 0.063
0.359TyrCys: 0.359 ± 0.022
2.346TyrAsp: 2.346 ± 0.064
3.208TyrGlu: 3.208 ± 0.071
1.733TyrPhe: 1.733 ± 0.047
2.555TyrGly: 2.555 ± 0.061
0.854TyrHis: 0.854 ± 0.041
2.62TyrIle: 2.62 ± 0.061
1.999TyrLys: 1.999 ± 0.06
2.715TyrLeu: 2.715 ± 0.059
1.067TyrMet: 1.067 ± 0.034
1.278TyrAsn: 1.278 ± 0.043
1.347TyrPro: 1.347 ± 0.041
1.169TyrGln: 1.169 ± 0.04
2.029TyrArg: 2.029 ± 0.053
1.67TyrSer: 1.67 ± 0.045
1.954TyrThr: 1.954 ± 0.051
3.163TyrVal: 3.163 ± 0.058
0.354TyrTrp: 0.354 ± 0.022
1.453TyrTyr: 1.453 ± 0.046
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2819 proteins (830520 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski