Amino acid dipepetide frequency for Enterocytozoon bieneusi (strain H348) (Microsporidian parasite)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.23AlaAla: 2.23 ± 0.096	0.723AlaCys: 0.723 ± 0.029	2.004AlaAsp: 2.004 ± 0.052	2.212AlaGlu: 2.212 ± 0.061	2.082AlaPhe: 2.082 ± 0.053	1.862AlaGly: 1.862 ± 0.055	0.823AlaHis: 0.823 ± 0.031	3.794AlaIle: 3.794 ± 0.071	3.055AlaLys: 3.055 ± 0.081	3.874AlaLeu: 3.874 ± 0.085	0.902AlaMet: 0.902 ± 0.031	2.273AlaAsn: 2.273 ± 0.051	1.174AlaPro: 1.174 ± 0.048	1.303AlaGln: 1.303 ± 0.051	1.537AlaArg: 1.537 ± 0.052	2.371AlaSer: 2.371 ± 0.072	2.074AlaThr: 2.074 ± 0.053	2.257AlaVal: 2.257 ± 0.057	0.36AlaTrp: 0.36 ± 0.023	1.454AlaTyr: 1.454 ± 0.049	0.007AlaXaa: 0.007 ± 0.003
Cys	0.743CysAla: 0.743 ± 0.032	0.402CysCys: 0.402 ± 0.023	1.029CysAsp: 1.029 ± 0.041	1.225CysGlu: 1.225 ± 0.043	0.971CysPhe: 0.971 ± 0.033	1.154CysGly: 1.154 ± 0.043	0.411CysHis: 0.411 ± 0.021	2.041CysIle: 2.041 ± 0.048	1.785CysLys: 1.785 ± 0.048	1.784CysLeu: 1.784 ± 0.042	0.392CysMet: 0.392 ± 0.024	1.376CysAsn: 1.376 ± 0.045	0.648CysPro: 0.648 ± 0.029	0.486CysGln: 0.486 ± 0.025	0.704CysArg: 0.704 ± 0.029	1.308CysSer: 1.308 ± 0.037	1.017CysThr: 1.017 ± 0.033	1.02CysVal: 1.02 ± 0.034	0.117CysTrp: 0.117 ± 0.011	0.78CysTyr: 0.78 ± 0.032	0.004CysXaa: 0.004 ± 0.002
Asp	2.143AspAla: 2.143 ± 0.059	0.872AspCys: 0.872 ± 0.037	3.174AspAsp: 3.174 ± 0.076	4.322AspGlu: 4.322 ± 0.089	2.914AspPhe: 2.914 ± 0.063	2.279AspGly: 2.279 ± 0.065	0.887AspHis: 0.887 ± 0.035	5.817AspIle: 5.817 ± 0.1	4.192AspLys: 4.192 ± 0.077	4.796AspLeu: 4.796 ± 0.085	1.207AspMet: 1.207 ± 0.039	3.69AspAsn: 3.69 ± 0.073	1.471AspPro: 1.471 ± 0.041	1.467AspGln: 1.467 ± 0.043	1.592AspArg: 1.592 ± 0.053	2.901AspSer: 2.901 ± 0.069	2.663AspThr: 2.663 ± 0.056	2.885AspVal: 2.885 ± 0.063	0.33AspTrp: 0.33 ± 0.021	2.222AspTyr: 2.222 ± 0.059	0.001AspXaa: 0.001 ± 0.001
Glu	2.419GluAla: 2.419 ± 0.058	1.305GluCys: 1.305 ± 0.047	3.077GluAsp: 3.077 ± 0.066	4.717GluGlu: 4.717 ± 0.1	3.511GluPhe: 3.511 ± 0.071	2.204GluGly: 2.204 ± 0.055	1.283GluHis: 1.283 ± 0.043	7.366GluIle: 7.366 ± 0.107	6.955GluLys: 6.955 ± 0.102	6.347GluLeu: 6.347 ± 0.102	1.748GluMet: 1.748 ± 0.048	5.32GluAsn: 5.32 ± 0.087	1.327GluPro: 1.327 ± 0.043	2.093GluGln: 2.093 ± 0.06	2.325GluArg: 2.325 ± 0.065	3.388GluSer: 3.388 ± 0.067	3.427GluThr: 3.427 ± 0.065	3.222GluVal: 3.222 ± 0.066	0.513GluTrp: 0.513 ± 0.025	3.444GluTyr: 3.444 ± 0.064	0.007GluXaa: 0.007 ± 0.003
Phe	1.73PheAla: 1.73 ± 0.046	1.143PheCys: 1.143 ± 0.042	3.066PheAsp: 3.066 ± 0.059	3.123PheGlu: 3.123 ± 0.068	3.407PhePhe: 3.407 ± 0.115	2.227PheGly: 2.227 ± 0.062	1.067PheHis: 1.067 ± 0.033	5.683PheIle: 5.683 ± 0.103	4.589PheLys: 4.589 ± 0.076	5.196PheLeu: 5.196 ± 0.089	1.295PheMet: 1.295 ± 0.045	4.094PheAsn: 4.094 ± 0.075	1.603PhePro: 1.603 ± 0.047	1.63PheGln: 1.63 ± 0.043	1.626PheArg: 1.626 ± 0.044	3.713PheSer: 3.713 ± 0.07	2.69PheThr: 2.69 ± 0.057	2.919PheVal: 2.919 ± 0.056	0.399PheTrp: 0.399 ± 0.023	3.063PheTyr: 3.063 ± 0.058	0.011PheXaa: 0.011 ± 0.004
Gly	1.728GlyAla: 1.728 ± 0.053	0.962GlyCys: 0.962 ± 0.033	1.939GlyAsp: 1.939 ± 0.051	2.668GlyGlu: 2.668 ± 0.065	2.287GlyPhe: 2.287 ± 0.06	2.44GlyGly: 2.44 ± 0.091	0.911GlyHis: 0.911 ± 0.034	4.703GlyIle: 4.703 ± 0.084	3.952GlyLys: 3.952 ± 0.076	3.663GlyLeu: 3.663 ± 0.067	1.211GlyMet: 1.211 ± 0.039	2.516GlyAsn: 2.516 ± 0.054	0.901GlyPro: 0.901 ± 0.031	1.253GlyGln: 1.253 ± 0.037	1.742GlyArg: 1.742 ± 0.05	2.632GlySer: 2.632 ± 0.073	2.28GlyThr: 2.28 ± 0.058	2.595GlyVal: 2.595 ± 0.066	0.301GlyTrp: 0.301 ± 0.02	1.805GlyTyr: 1.805 ± 0.053	0.007GlyXaa: 0.007 ± 0.003
His	0.78HisAla: 0.78 ± 0.032	0.408HisCys: 0.408 ± 0.025	0.849HisAsp: 0.849 ± 0.036	1.225HisGlu: 1.225 ± 0.043	1.249HisPhe: 1.249 ± 0.038	1.054HisGly: 1.054 ± 0.04	0.516HisHis: 0.516 ± 0.026	1.969HisIle: 1.969 ± 0.054	1.58HisLys: 1.58 ± 0.05	1.751HisLeu: 1.751 ± 0.05	0.462HisMet: 0.462 ± 0.026	1.386HisAsn: 1.386 ± 0.044	0.925HisPro: 0.925 ± 0.033	0.744HisGln: 0.744 ± 0.027	0.868HisArg: 0.868 ± 0.034	1.488HisSer: 1.488 ± 0.048	1.127HisThr: 1.127 ± 0.045	0.908HisVal: 0.908 ± 0.036	0.123HisTrp: 0.123 ± 0.013	0.841HisTyr: 0.841 ± 0.033	0.001HisXaa: 0.001 ± 0.001
Ile	3.35IleAla: 3.35 ± 0.072	2.071IleCys: 2.071 ± 0.055	5.807IleAsp: 5.807 ± 0.109	7.006IleGlu: 7.006 ± 0.116	5.689IlePhe: 5.689 ± 0.087	3.909IleGly: 3.909 ± 0.071	1.935IleHis: 1.935 ± 0.049	10.259IleIle: 10.259 ± 0.159	9.562IleLys: 9.562 ± 0.142	9.69IleLeu: 9.69 ± 0.135	1.998IleMet: 1.998 ± 0.051	8.106IleAsn: 8.106 ± 0.135	3.357IlePro: 3.357 ± 0.063	4.001IleGln: 4.001 ± 0.075	3.503IleArg: 3.503 ± 0.069	6.828IleSer: 6.828 ± 0.089	5.288IleThr: 5.288 ± 0.086	5.646IleVal: 5.646 ± 0.097	0.599IleTrp: 0.599 ± 0.025	4.679IleTyr: 4.679 ± 0.085	0.008IleXaa: 0.008 ± 0.003
Lys	2.962LysAla: 2.962 ± 0.063	1.687LysCys: 1.687 ± 0.047	4.281LysAsp: 4.281 ± 0.071	6.309LysGlu: 6.309 ± 0.092	3.952LysPhe: 3.952 ± 0.073	3.053LysGly: 3.053 ± 0.066	1.935LysHis: 1.935 ± 0.058	10.204LysIle: 10.204 ± 0.148	10.205LysLys: 10.205 ± 0.165	8.285LysLeu: 8.285 ± 0.112	2.498LysMet: 2.498 ± 0.055	8.005LysAsn: 8.005 ± 0.117	2.299LysPro: 2.299 ± 0.069	3.067LysGln: 3.067 ± 0.068	2.844LysArg: 2.844 ± 0.066	5.241LysSer: 5.241 ± 0.082	5.666LysThr: 5.666 ± 0.082	3.81LysVal: 3.81 ± 0.069	0.611LysTrp: 0.611 ± 0.025	4.994LysTyr: 4.994 ± 0.085	0.002LysXaa: 0.002 ± 0.002
Leu	3.63LeuAla: 3.63 ± 0.082	1.87LeuCys: 1.87 ± 0.04	4.706LeuAsp: 4.706 ± 0.076	6.218LeuGlu: 6.218 ± 0.091	5.652LeuPhe: 5.652 ± 0.11	3.915LeuGly: 3.915 ± 0.071	1.952LeuHis: 1.952 ± 0.051	8.544LeuIle: 8.544 ± 0.133	9.073LeuLys: 9.073 ± 0.1	8.948LeuLeu: 8.948 ± 0.118	2.027LeuMet: 2.027 ± 0.049	7.227LeuAsn: 7.227 ± 0.107	2.657LeuPro: 2.657 ± 0.063	3.317LeuGln: 3.317 ± 0.07	3.214LeuArg: 3.214 ± 0.068	6.025LeuSer: 6.025 ± 0.095	4.322LeuThr: 4.322 ± 0.082	4.402LeuVal: 4.402 ± 0.075	0.758LeuTrp: 0.758 ± 0.025	4.265LeuTyr: 4.265 ± 0.076	0.012LeuXaa: 0.012 ± 0.004
Met	1.153MetAla: 1.153 ± 0.039	0.553MetCys: 0.553 ± 0.027	1.242MetAsp: 1.242 ± 0.039	1.653MetGlu: 1.653 ± 0.047	1.266MetPhe: 1.266 ± 0.038	1.101MetGly: 1.101 ± 0.036	0.605MetHis: 0.605 ± 0.03	2.149MetIle: 2.149 ± 0.052	2.062MetLys: 2.062 ± 0.054	2.064MetLeu: 2.064 ± 0.048	0.506MetMet: 0.506 ± 0.026	1.583MetAsn: 1.583 ± 0.043	0.634MetPro: 0.634 ± 0.025	0.801MetGln: 0.801 ± 0.028	0.813MetArg: 0.813 ± 0.035	1.499MetSer: 1.499 ± 0.042	1.119MetThr: 1.119 ± 0.035	1.38MetVal: 1.38 ± 0.043	0.175MetTrp: 0.175 ± 0.013	1.106MetTyr: 1.106 ± 0.041	0.002MetXaa: 0.002 ± 0.001
Asn	2.662AsnAla: 2.662 ± 0.049	1.323AsnCys: 1.323 ± 0.047	4.223AsnAsp: 4.223 ± 0.079	5.022AsnGlu: 5.022 ± 0.091	3.551AsnPhe: 3.551 ± 0.075	2.755AsnGly: 2.755 ± 0.059	1.347AsnHis: 1.347 ± 0.041	8.891AsnIle: 8.891 ± 0.149	7.511AsnLys: 7.511 ± 0.133	6.372AsnLeu: 6.372 ± 0.099	1.855AsnMet: 1.855 ± 0.044	7.162AsnAsn: 7.162 ± 0.158	2.296AsnPro: 2.296 ± 0.053	2.314AsnGln: 2.314 ± 0.052	2.216AsnArg: 2.216 ± 0.053	4.512AsnSer: 4.512 ± 0.084	4.71AsnThr: 4.71 ± 0.104	3.412AsnVal: 3.412 ± 0.068	0.443AsnTrp: 0.443 ± 0.026	3.71AsnTyr: 3.71 ± 0.071	0.005AsnXaa: 0.005 ± 0.002
Pro	1.115ProAla: 1.115 ± 0.057	0.538ProCys: 0.538 ± 0.025	1.493ProAsp: 1.493 ± 0.049	2.028ProGlu: 2.028 ± 0.053	1.645ProPhe: 1.645 ± 0.047	1.518ProGly: 1.518 ± 0.05	0.665ProHis: 0.665 ± 0.028	2.819ProIle: 2.819 ± 0.062	2.116ProLys: 2.116 ± 0.06	2.62ProLeu: 2.62 ± 0.057	0.676ProMet: 0.676 ± 0.029	1.869ProAsn: 1.869 ± 0.046	1.323ProPro: 1.323 ± 0.078	1.047ProGln: 1.047 ± 0.035	1.148ProArg: 1.148 ± 0.039	2.146ProSer: 2.146 ± 0.056	1.77ProThr: 1.77 ± 0.054	1.575ProVal: 1.575 ± 0.052	0.229ProTrp: 0.229 ± 0.015	1.108ProTyr: 1.108 ± 0.038	0.007ProXaa: 0.007 ± 0.003
Gln	1.298GlnAla: 1.298 ± 0.044	0.639GlnCys: 0.639 ± 0.028	1.395GlnAsp: 1.395 ± 0.047	2.027GlnGlu: 2.027 ± 0.048	1.944GlnPhe: 1.944 ± 0.045	1.187GlnGly: 1.187 ± 0.038	0.791GlnHis: 0.791 ± 0.033	3.446GlnIle: 3.446 ± 0.072	3.117GlnLys: 3.117 ± 0.064	3.517GlnLeu: 3.517 ± 0.07	0.842GlnMet: 0.842 ± 0.034	2.503GlnAsn: 2.503 ± 0.057	0.823GlnPro: 0.823 ± 0.034	1.254GlnGln: 1.254 ± 0.044	1.32GlnArg: 1.32 ± 0.046	2.06GlnSer: 2.06 ± 0.045	1.866GlnThr: 1.866 ± 0.049	1.433GlnVal: 1.433 ± 0.046	0.283GlnTrp: 0.283 ± 0.02	1.565GlnTyr: 1.565 ± 0.045	0.002GlnXaa: 0.002 ± 0.001
Arg	1.528ArgAla: 1.528 ± 0.047	0.67ArgCys: 0.67 ± 0.028	1.655ArgAsp: 1.655 ± 0.053	2.351ArgGlu: 2.351 ± 0.06	1.862ArgPhe: 1.862 ± 0.051	1.767ArgGly: 1.767 ± 0.058	0.743ArgHis: 0.743 ± 0.034	3.39ArgIle: 3.39 ± 0.069	3.069ArgLys: 3.069 ± 0.067	3.221ArgLeu: 3.221 ± 0.06	0.958ArgMet: 0.958 ± 0.033	2.351ArgAsn: 2.351 ± 0.063	0.907ArgPro: 0.907 ± 0.034	1.226ArgGln: 1.226 ± 0.043	1.689ArgArg: 1.689 ± 0.055	2.103ArgSer: 2.103 ± 0.053	1.586ArgThr: 1.586 ± 0.052	1.869ArgVal: 1.869 ± 0.049	0.365ArgTrp: 0.365 ± 0.021	1.572ArgTyr: 1.572 ± 0.038	0.006ArgXaa: 0.006 ± 0.003
Ser	2.518SerAla: 2.518 ± 0.069	1.043SerCys: 1.043 ± 0.036	3.498SerAsp: 3.498 ± 0.068	4.068SerGlu: 4.068 ± 0.07	3.133SerPhe: 3.133 ± 0.055	3.048SerGly: 3.048 ± 0.075	1.196SerHis: 1.196 ± 0.036	6.499SerIle: 6.499 ± 0.098	5.623SerLys: 5.623 ± 0.088	5.951SerLeu: 5.951 ± 0.102	1.439SerMet: 1.439 ± 0.044	4.703SerAsn: 4.703 ± 0.093	2.036SerPro: 2.036 ± 0.052	2.188SerGln: 2.188 ± 0.053	2.301SerArg: 2.301 ± 0.06	4.405SerSer: 4.405 ± 0.084	3.752SerThr: 3.752 ± 0.085	3.21SerVal: 3.21 ± 0.061	0.387SerTrp: 0.387 ± 0.022	2.337SerTyr: 2.337 ± 0.052	0.007SerXaa: 0.007 ± 0.003
Thr	2.057ThrAla: 2.057 ± 0.057	0.934ThrCys: 0.934 ± 0.038	2.562ThrAsp: 2.562 ± 0.058	3.105ThrGlu: 3.105 ± 0.058	2.755ThrPhe: 2.755 ± 0.055	2.373ThrGly: 2.373 ± 0.06	1.145ThrHis: 1.145 ± 0.037	5.998ThrIle: 5.998 ± 0.097	4.391ThrLys: 4.391 ± 0.075	4.899ThrLeu: 4.899 ± 0.073	1.164ThrMet: 1.164 ± 0.035	4.148ThrAsn: 4.148 ± 0.07	2.124ThrPro: 2.124 ± 0.057	1.92ThrGln: 1.92 ± 0.053	1.635ThrArg: 1.635 ± 0.048	3.664ThrSer: 3.664 ± 0.073	3.676ThrThr: 3.676 ± 0.079	2.881ThrVal: 2.881 ± 0.065	0.405ThrTrp: 0.405 ± 0.019	2.184ThrTyr: 2.184 ± 0.058	0.004ThrXaa: 0.004 ± 0.002
Val	2.233ValAla: 2.233 ± 0.058	1.099ValCys: 1.099 ± 0.038	2.92ValAsp: 2.92 ± 0.066	3.603ValGlu: 3.603 ± 0.069	3.28ValPhe: 3.28 ± 0.074	2.326ValGly: 2.326 ± 0.061	1.079ValHis: 1.079 ± 0.036	4.53ValIle: 4.53 ± 0.075	4.099ValLys: 4.099 ± 0.086	4.82ValLeu: 4.82 ± 0.086	1.072ValMet: 1.072 ± 0.036	3.507ValAsn: 3.507 ± 0.065	1.528ValPro: 1.528 ± 0.046	1.581ValGln: 1.581 ± 0.042	1.831ValArg: 1.831 ± 0.054	3.411ValSer: 3.411 ± 0.066	2.354ValThr: 2.354 ± 0.051	3.111ValVal: 3.111 ± 0.074	0.494ValTrp: 0.494 ± 0.027	2.303ValTyr: 2.303 ± 0.055	0.004ValXaa: 0.004 ± 0.002
Trp	0.346TrpAla: 0.346 ± 0.024	0.239TrpCys: 0.239 ± 0.021	0.377TrpAsp: 0.377 ± 0.021	0.431TrpGlu: 0.431 ± 0.025	0.359TrpPhe: 0.359 ± 0.02	0.504TrpGly: 0.504 ± 0.023	0.148TrpHis: 0.148 ± 0.014	0.716TrpIle: 0.716 ± 0.029	0.611TrpLys: 0.611 ± 0.03	0.575TrpLeu: 0.575 ± 0.027	0.236TrpMet: 0.236 ± 0.016	0.584TrpAsn: 0.584 ± 0.027	0.212TrpPro: 0.212 ± 0.017	0.139TrpGln: 0.139 ± 0.014	0.354TrpArg: 0.354 ± 0.017	0.448TrpSer: 0.448 ± 0.024	0.267TrpThr: 0.267 ± 0.02	0.337TrpVal: 0.337 ± 0.022	0.062TrpTrp: 0.062 ± 0.008	0.352TrpTyr: 0.352 ± 0.023	0.001TrpXaa: 0.001 ± 0.001
Tyr	1.739TyrAla: 1.739 ± 0.043	0.916TyrCys: 0.916 ± 0.04	2.524TyrAsp: 2.524 ± 0.056	2.672TyrGlu: 2.672 ± 0.067	2.76TyrPhe: 2.76 ± 0.06	1.812TyrGly: 1.812 ± 0.045	0.812TyrHis: 0.812 ± 0.033	4.466TyrIle: 4.466 ± 0.084	4.354TyrLys: 4.354 ± 0.077	4.353TyrLeu: 4.353 ± 0.074	0.972TyrMet: 0.972 ± 0.031	3.879TyrAsn: 3.879 ± 0.08	1.21TyrPro: 1.21 ± 0.038	1.477TyrGln: 1.477 ± 0.044	1.622TyrArg: 1.622 ± 0.051	3.194TyrSer: 3.194 ± 0.068	2.362TyrThr: 2.362 ± 0.056	2.305TyrVal: 2.305 ± 0.053	0.344TyrTrp: 0.344 ± 0.021	2.267TyrTyr: 2.267 ± 0.052	0.01TyrXaa: 0.01 ± 0.003
Xaa	0.007XaaAla: 0.007 ± 0.003	0.001XaaCys: 0.001 ± 0.001	0.004XaaAsp: 0.004 ± 0.002	0.01XaaGlu: 0.01 ± 0.003	0.004XaaPhe: 0.004 ± 0.002	0.007XaaGly: 0.007 ± 0.003	0.005XaaHis: 0.005 ± 0.002	0.006XaaIle: 0.006 ± 0.003	0.01XaaLys: 0.01 ± 0.005	0.01XaaLeu: 0.01 ± 0.004	0.004XaaMet: 0.004 ± 0.002	0.011XaaAsn: 0.011 ± 0.004	0.001XaaPro: 0.001 ± 0.001	0.002XaaGln: 0.002 ± 0.002	0.007XaaArg: 0.007 ± 0.003	0.007XaaSer: 0.007 ± 0.003	0.007XaaThr: 0.007 ± 0.003	0.006XaaVal: 0.006 ± 0.003	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.022XaaXaa: 0.022 ± 0.01

Statistics based on 3311 proteins (826748 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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