Mobiluncus curtisii (strain ATCC 43063 / DSM 2711 / V125) (Falcivibrio vaginalis)
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1904 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6ZFU9|D6ZFU9_MOBCV Transcriptional regulator IclR family C-terminal domain protein OS=Mobiluncus curtisii (strain ATCC 43063 / DSM 2711 / V125) OX=548479 GN=HMPREF0573_11188 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 9.75 AQNPALFGKK11 pKa = 10.16 QPATLLTVKK20 pKa = 10.25 NGKK23 pKa = 8.79 DD24 pKa = 2.84 RR25 pKa = 11.84 HH26 pKa = 5.31 MSEE29 pKa = 5.01 AAPQLLTSEE38 pKa = 5.64 DD39 pKa = 3.29 IFSAQFPATKK49 pKa = 10.13 FRR51 pKa = 11.84 DD52 pKa = 4.43 GYY54 pKa = 10.67 DD55 pKa = 3.03 QNQIDD60 pKa = 4.67 DD61 pKa = 4.11 YY62 pKa = 10.39 LDD64 pKa = 3.29 EE65 pKa = 4.38 VVRR68 pKa = 11.84 VLSYY72 pKa = 11.35 YY73 pKa = 10.02 EE74 pKa = 4.37 ALNASPEE81 pKa = 4.15 AEE83 pKa = 3.87 VDD85 pKa = 3.38 LAYY88 pKa = 9.33 ITVRR92 pKa = 11.84 GRR94 pKa = 11.84 DD95 pKa = 3.45 VRR97 pKa = 11.84 EE98 pKa = 3.31 VDD100 pKa = 3.83 FDD102 pKa = 3.77 YY103 pKa = 11.3 TRR105 pKa = 11.84 MRR107 pKa = 11.84 VGYY110 pKa = 9.8 DD111 pKa = 2.92 QDD113 pKa = 4.8 AVDD116 pKa = 5.52 DD117 pKa = 4.27 YY118 pKa = 11.24 LDD120 pKa = 3.46 QVAATLEE127 pKa = 4.44 AYY129 pKa = 7.77 EE130 pKa = 4.45 KK131 pKa = 10.77 LYY133 pKa = 10.72 GIPPSDD139 pKa = 3.23 QRR141 pKa = 11.84 YY142 pKa = 8.28 QVLQVDD148 pKa = 5.16 GAALEE153 pKa = 4.51 AEE155 pKa = 4.48 AAEE158 pKa = 4.15 QAAASGEE165 pKa = 4.36 LPSSSAPNEE174 pKa = 4.15 DD175 pKa = 3.61 AAVTPIEE182 pKa = 4.49 IAAEE186 pKa = 4.26 TPPSQPAEE194 pKa = 3.95 QTVASAAPLPVTPAATPSEE213 pKa = 4.14 DD214 pKa = 3.65 LGVSSSILGTPTGSIPVTQPVPFEE238 pKa = 3.84 QAVLTNHH245 pKa = 6.78 EE246 pKa = 4.79 DD247 pKa = 3.58 GSPAGHH253 pKa = 6.48 EE254 pKa = 4.22 FDD256 pKa = 3.9 ATSTASSPLFPPLNAAMSPQSQMPEE281 pKa = 3.8 TGTNASPVEE290 pKa = 4.56 SIPEE294 pKa = 3.76 QNFASAALPEE304 pKa = 4.68 TEE306 pKa = 4.36 TPIAPSVAASGEE318 pKa = 4.16 TAPAVPLPEE327 pKa = 5.67 PDD329 pKa = 4.6 DD330 pKa = 3.62 EE331 pKa = 4.5 WGEE334 pKa = 4.29 VPQYY338 pKa = 10.56 PVDD341 pKa = 3.74 APEE344 pKa = 4.43 PQTPDD349 pKa = 2.48 ATAAQTYY356 pKa = 9.51 SEE358 pKa = 4.81 PGYY361 pKa = 10.82 DD362 pKa = 3.2 PYY364 pKa = 11.36 QPYY367 pKa = 10.39 EE368 pKa = 4.03 YY369 pKa = 10.27 DD370 pKa = 4.8 AYY372 pKa = 10.1 DD373 pKa = 3.59 QPMSTAPAEE382 pKa = 4.29 YY383 pKa = 9.62 QGSSEE388 pKa = 4.92 PYY390 pKa = 9.31 PEE392 pKa = 4.96 APTEE396 pKa = 3.95 AGYY399 pKa = 9.57 PSPYY403 pKa = 10.26 GAATPEE409 pKa = 4.82 GYY411 pKa = 10.09 DD412 pKa = 3.55 YY413 pKa = 10.88 PPQAEE418 pKa = 4.54 GEE420 pKa = 4.22 NQAFIPEE427 pKa = 4.42 DD428 pKa = 3.41 TTMVIPVTPAAPVDD442 pKa = 3.71 TAPPTEE448 pKa = 4.37 VPSEE452 pKa = 3.97 YY453 pKa = 10.88 AQDD456 pKa = 4.07 YY457 pKa = 10.52 EE458 pKa = 4.19 PGEE461 pKa = 4.15 YY462 pKa = 9.95 PEE464 pKa = 5.28 GDD466 pKa = 3.14 EE467 pKa = 4.54 SSSEE471 pKa = 3.97 EE472 pKa = 4.16 TPDD475 pKa = 3.45 YY476 pKa = 11.39 DD477 pKa = 3.58 STFAVDD483 pKa = 3.71 QEE485 pKa = 4.27 EE486 pKa = 4.89 PYY488 pKa = 11.07 DD489 pKa = 4.16 LEE491 pKa = 4.37 EE492 pKa = 5.4 SLDD495 pKa = 3.62 DD496 pKa = 5.47 DD497 pKa = 4.17 YY498 pKa = 11.9 PEE500 pKa = 5.78 EE501 pKa = 4.17 ITLDD505 pKa = 3.98 DD506 pKa = 4.94 APDD509 pKa = 3.93 LPVEE513 pKa = 4.43 TTDD516 pKa = 3.4 VQADD520 pKa = 3.69 STALQEE526 pKa = 4.96 DD527 pKa = 5.88 FYY529 pKa = 11.93 APATPEE535 pKa = 4.17 EE536 pKa = 4.63 TPVAPVAAEE545 pKa = 3.66 PAEE548 pKa = 4.34 YY549 pKa = 9.93 YY550 pKa = 10.82 APEE553 pKa = 4.25 SVPEE557 pKa = 4.7 SISQPYY563 pKa = 8.71 VEE565 pKa = 5.19 QPQPPYY571 pKa = 9.73 ATPEE575 pKa = 4.1 SPTIDD580 pKa = 3.86 PLTYY584 pKa = 9.39 ATPEE588 pKa = 3.87 QPAATDD594 pKa = 2.93 SWQTPNPYY602 pKa = 9.38 QAEE605 pKa = 4.2 GLTPSEE611 pKa = 4.07 WDD613 pKa = 3.62 GTPQPPNYY621 pKa = 9.64 EE622 pKa = 4.04 QFDD625 pKa = 4.21 EE626 pKa = 4.52 QPSVQPDD633 pKa = 3.92 LQPTDD638 pKa = 3.56 QYY640 pKa = 11.69 SVPDD644 pKa = 4.89 FSANEE649 pKa = 3.82 PAYY652 pKa = 9.61 ATAAPEE658 pKa = 4.17 MPEE661 pKa = 3.95 EE662 pKa = 4.11 LAVEE666 pKa = 4.27 QPEE669 pKa = 4.47 PTSEE673 pKa = 4.08 TLDD676 pKa = 3.71 APTEE680 pKa = 3.84 LSEE683 pKa = 4.14 PVEE686 pKa = 4.18 AAEE689 pKa = 4.05 PAEE692 pKa = 4.3 PSFSTFQMPQLEE704 pKa = 4.09 AVEE707 pKa = 4.3 LGKK710 pKa = 10.69 DD711 pKa = 3.25 EE712 pKa = 4.26 VAPVEE717 pKa = 4.33 VNDD720 pKa = 5.34 AEE722 pKa = 4.41 FAPADD727 pKa = 3.63 YY728 pKa = 8.4 STPTEE733 pKa = 4.22 TPKK736 pKa = 11.14 APLTDD741 pKa = 3.72 VVAPPLPTPGVGLDD755 pKa = 3.53 LGQIRR760 pKa = 11.84 EE761 pKa = 4.21 KK762 pKa = 10.65 LAPEE766 pKa = 4.01 QADD769 pKa = 3.34 SSAEE773 pKa = 4.09 SEE775 pKa = 4.29 QKK777 pKa = 10.78 LADD780 pKa = 3.66 VPGVLMTPTRR790 pKa = 11.84 SATDD794 pKa = 3.78 FPPTEE799 pKa = 4.61 SPTEE803 pKa = 4.16 SPAAGALTEE812 pKa = 4.29 PEE814 pKa = 3.93 PTGTVTEE821 pKa = 4.49 HH822 pKa = 6.93 LSATRR827 pKa = 11.84 LEE829 pKa = 4.25 EE830 pKa = 4.6 LAVQNAASVPEE841 pKa = 4.11 TAADD845 pKa = 3.18 IPMIDD850 pKa = 3.53 TQSEE854 pKa = 4.38 NNEE857 pKa = 4.22 STKK860 pKa = 10.73 GQPTPIFPDD869 pKa = 3.7 DD870 pKa = 4.07 SNPRR874 pKa = 11.84 PMLQGEE880 pKa = 4.36 EE881 pKa = 4.17 PLAEE885 pKa = 4.07 VDD887 pKa = 3.05 PDD889 pKa = 3.53 GRR891 pKa = 11.84 FIPHH895 pKa = 6.66 FLAGYY900 pKa = 9.72 RR901 pKa = 11.84 SNLDD905 pKa = 3.21 TFTSVYY911 pKa = 10.72 GSLDD915 pKa = 3.42 EE916 pKa = 4.54 SGSRR920 pKa = 11.84 VKK922 pKa = 10.19 PASIAKK928 pKa = 10.29 LEE930 pKa = 4.23 AQQHH934 pKa = 5.49 RR935 pKa = 11.84 KK936 pKa = 10.16 SITTGYY942 pKa = 9.76 LVTVATSRR950 pKa = 11.84 PLGADD955 pKa = 3.0 DD956 pKa = 3.73 QVFVRR961 pKa = 11.84 LPDD964 pKa = 3.57 GRR966 pKa = 11.84 EE967 pKa = 3.93 VPVTSASSDD976 pKa = 3.28 FDD978 pKa = 4.05 GVHH981 pKa = 5.48 LTIPKK986 pKa = 9.7 II987 pKa = 3.71
Molecular weight: 105.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.579
IPC_protein 3.605
Toseland 3.389
ProMoST 3.732
Dawson 3.567
Bjellqvist 3.719
Wikipedia 3.465
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.528
Nozaki 3.681
DTASelect 3.859
Thurlkill 3.427
EMBOSS 3.478
Sillero 3.719
Patrickios 0.617
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.662
Protein with the highest isoelectric point:
>tr|D6ZJH7|D6ZJH7_MOBCV Uncharacterized protein OS=Mobiluncus curtisii (strain ATCC 43063 / DSM 2711 / V125) OX=548479 GN=HMPREF0573_10557 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1904
0
1904
641598
32
5040
337.0
36.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.114 ± 0.072
0.861 ± 0.017
5.521 ± 0.04
5.892 ± 0.056
3.329 ± 0.033
8.113 ± 0.046
1.97 ± 0.026
4.995 ± 0.044
3.988 ± 0.058
9.9 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.315 ± 0.026
3.214 ± 0.038
5.246 ± 0.053
3.854 ± 0.032
5.774 ± 0.056
6.034 ± 0.039
6.183 ± 0.057
7.908 ± 0.045
1.404 ± 0.024
2.385 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here