Oceanimonas sp. (strain GK1 / IBRC-M 10197)
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3190 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H2FWV9|H2FWV9_OCESG Lipopolysaccharide assembly protein B OS=Oceanimonas sp. (strain GK1 / IBRC-M 10197) OX=511062 GN=lapB PE=3 SV=1
MM1 pKa = 7.68 KK2 pKa = 9.38 KK3 pKa = 9.08 TILALTIPALFATSASAVTVYY24 pKa = 10.62 SDD26 pKa = 3.61 EE27 pKa = 4.27 GAQVDD32 pKa = 3.41 IYY34 pKa = 11.54 GRR36 pKa = 11.84 VQFDD40 pKa = 3.28 GGEE43 pKa = 4.17 LDD45 pKa = 4.27 HH46 pKa = 7.83 QNDD49 pKa = 4.13 SNGKK53 pKa = 6.77 EE54 pKa = 4.12 VKK56 pKa = 10.19 SEE58 pKa = 4.1 SFGTDD63 pKa = 2.56 GSARR67 pKa = 11.84 LGVNMSYY74 pKa = 11.5 ALNNDD79 pKa = 2.44 VDD81 pKa = 5.52 LIGKK85 pKa = 8.14 LEE87 pKa = 3.96 WQVAAEE93 pKa = 4.49 ASDD96 pKa = 3.79 DD97 pKa = 4.05 SKK99 pKa = 11.67 FDD101 pKa = 4.46 ARR103 pKa = 11.84 YY104 pKa = 8.28 AWAGFRR110 pKa = 11.84 FMDD113 pKa = 3.7 TTEE116 pKa = 4.0 LTFGRR121 pKa = 11.84 SVDD124 pKa = 3.76 PLAQAIYY131 pKa = 8.87 LTDD134 pKa = 3.23 VFNIFGAGITYY145 pKa = 10.21 GSEE148 pKa = 3.78 FSISDD153 pKa = 3.6 KK154 pKa = 11.43 ADD156 pKa = 3.48 DD157 pKa = 4.77 QIMATYY163 pKa = 9.53 AANGVDD169 pKa = 4.62 LRR171 pKa = 11.84 AAYY174 pKa = 10.09 AFADD178 pKa = 4.12 DD179 pKa = 5.3 DD180 pKa = 3.88 RR181 pKa = 11.84 TDD183 pKa = 3.91 FANQANGDD191 pKa = 3.68 VAEE194 pKa = 4.37 NQWAVSAGYY203 pKa = 7.48 TFPFGLGLVAAYY215 pKa = 7.42 EE216 pKa = 4.06 QQNGYY221 pKa = 9.75 EE222 pKa = 4.33 KK223 pKa = 10.37 TGLTSQNIDD232 pKa = 2.94 QDD234 pKa = 3.02 VWYY237 pKa = 10.45 AGVHH241 pKa = 4.1 YY242 pKa = 9.04 TLDD245 pKa = 3.35 GFYY248 pKa = 10.51 FAALYY253 pKa = 10.55 SDD255 pKa = 4.03 RR256 pKa = 11.84 QRR258 pKa = 11.84 DD259 pKa = 3.45 TGTAAGDD266 pKa = 4.03 DD267 pKa = 3.68 EE268 pKa = 4.45 GTAYY272 pKa = 9.95 EE273 pKa = 4.8 LHH275 pKa = 6.15 AQYY278 pKa = 11.21 NVDD281 pKa = 3.51 AWTLMAQYY289 pKa = 10.69 SKK291 pKa = 11.59 EE292 pKa = 4.09 EE293 pKa = 3.8 FDD295 pKa = 3.62 AAGATEE301 pKa = 4.96 EE302 pKa = 4.58 YY303 pKa = 11.0 DD304 pKa = 3.95 SIDD307 pKa = 4.97 DD308 pKa = 3.53 ITLGVQYY315 pKa = 11.16 DD316 pKa = 4.09 LTSKK320 pKa = 9.03 TKK322 pKa = 10.55 LYY324 pKa = 10.83 AEE326 pKa = 4.29 YY327 pKa = 10.29 VISDD331 pKa = 4.25 SEE333 pKa = 4.35 VDD335 pKa = 3.16 TSGAKK340 pKa = 9.8 KK341 pKa = 10.35 DD342 pKa = 3.65 DD343 pKa = 4.02 LYY345 pKa = 11.73 GVGIQYY351 pKa = 10.53 NFF353 pKa = 3.35
Molecular weight: 38.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.668
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.846
Sillero 4.012
Patrickios 1.38
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|H2FYZ9|H2FYZ9_OCESG RNA-binding protein Hfq OS=Oceanimonas sp. (strain GK1 / IBRC-M 10197) OX=511062 GN=hfq PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.29 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3190
0
3190
1032523
32
2846
323.7
35.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.771 ± 0.055
1.021 ± 0.014
5.226 ± 0.032
6.145 ± 0.039
3.587 ± 0.031
8.029 ± 0.042
2.477 ± 0.022
4.727 ± 0.036
3.293 ± 0.038
12.149 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.022
3.041 ± 0.02
4.713 ± 0.028
4.668 ± 0.04
6.547 ± 0.048
5.263 ± 0.033
4.849 ± 0.034
7.009 ± 0.04
1.418 ± 0.018
2.515 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here