Sulfitobacter phage pCB2047-C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4NUR9|M4NUR9_9CAUD Hemolysin Xh1A-like protein OS=Sulfitobacter phage pCB2047-C OX=754043 GN=SUBG_00018 PE=4 SV=1
MM1 pKa = 8.08LYY3 pKa = 10.24RR4 pKa = 11.84IQSTALGQSGNTLLILPNATITVRR28 pKa = 11.84DD29 pKa = 3.78ANGDD33 pKa = 3.89LARR36 pKa = 11.84LYY38 pKa = 10.94ADD40 pKa = 3.99PDD42 pKa = 4.21EE43 pKa = 4.53ITEE46 pKa = 4.31IPNPTLSDD54 pKa = 3.21AAGGYY59 pKa = 10.27DD60 pKa = 3.83FYY62 pKa = 11.91VEE64 pKa = 3.98YY65 pKa = 11.01GDD67 pKa = 4.66YY68 pKa = 11.35YY69 pKa = 11.18DD70 pKa = 4.17ITVALNGQSVDD81 pKa = 3.47DD82 pKa = 3.89RR83 pKa = 11.84VYY85 pKa = 10.64PIDD88 pKa = 3.62MGLGARR94 pKa = 11.84VDD96 pKa = 3.63AAAATATEE104 pKa = 4.13QAGIATTKK112 pKa = 10.44AGEE115 pKa = 4.38SNASAVTSQAWAEE128 pKa = 4.19SDD130 pKa = 3.76TAPGDD135 pKa = 3.89PGTKK139 pKa = 9.41SAKK142 pKa = 8.04TWAGEE147 pKa = 3.95AAGDD151 pKa = 3.59ATAAAGSAAKK161 pKa = 10.54AEE163 pKa = 4.54LFDD166 pKa = 4.09GPKK169 pKa = 10.24FNTIALMALYY179 pKa = 9.86EE180 pKa = 4.15DD181 pKa = 4.65AKK183 pKa = 10.97AGDD186 pKa = 3.72VATVLSAFNGGVEE199 pKa = 3.95HH200 pKa = 7.07FDD202 pKa = 3.64WIEE205 pKa = 3.86GVAA208 pKa = 3.72

Molecular weight: 21.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4NNI6|M4NNI6_9CAUD Uncharacterized protein OS=Sulfitobacter phage pCB2047-C OX=754043 GN=SUBG_00046 PE=4 SV=1
MM1 pKa = 7.1MHH3 pKa = 7.12DD4 pKa = 3.26QAQFKK9 pKa = 10.66RR10 pKa = 11.84PTGYY14 pKa = 10.25RR15 pKa = 11.84GMALTQLMDD24 pKa = 3.33RR25 pKa = 11.84RR26 pKa = 11.84PFKK29 pKa = 10.6RR30 pKa = 11.84GSADD34 pKa = 2.72WEE36 pKa = 4.31YY37 pKa = 10.24RR38 pKa = 11.84TIAAWVYY45 pKa = 10.01LQMAMGRR52 pKa = 11.84ASCDD56 pKa = 2.77WTRR59 pKa = 11.84TPPRR63 pKa = 11.84VTVGAAQQ70 pKa = 3.14

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

12774

31

719

175.0

19.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.361 ± 0.461

0.955 ± 0.14

6.764 ± 0.283

5.762 ± 0.336

3.108 ± 0.152

8.807 ± 0.557

1.996 ± 0.209

4.47 ± 0.217

4.47 ± 0.287

7.374 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.193

3.648 ± 0.207

4.407 ± 0.218

4.274 ± 0.395

6.537 ± 0.374

5.879 ± 0.276

5.801 ± 0.328

6.294 ± 0.316

1.738 ± 0.207

2.529 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski