Paracoccus sp. BM15

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus; unclassified Paracoccus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3740 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9EEP5|A0A2K9EEP5_9RHOB 3-mercaptopyruvate sulfurtransferase OS=Paracoccus sp. BM15 OX=1529068 GN=CUV01_08485 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.19KK3 pKa = 10.41VLFATTALVMTAGVAAAEE21 pKa = 4.39VSLSGDD27 pKa = 2.74GRR29 pKa = 11.84MGMVYY34 pKa = 10.52DD35 pKa = 4.8GDD37 pKa = 4.47DD38 pKa = 3.41LQFTSRR44 pKa = 11.84ARR46 pKa = 11.84VKK48 pKa = 9.79FTLTGEE54 pKa = 4.24SDD56 pKa = 3.24SGLSFGGEE64 pKa = 3.52FRR66 pKa = 11.84VDD68 pKa = 4.06HH69 pKa = 6.7EE70 pKa = 5.26DD71 pKa = 3.41EE72 pKa = 5.52DD73 pKa = 4.69GSSASRR79 pKa = 11.84GGAGHH84 pKa = 6.52VYY86 pKa = 10.41ISGAYY91 pKa = 9.13GKK93 pKa = 10.77LSMGDD98 pKa = 2.88IDD100 pKa = 4.85SASEE104 pKa = 3.88KK105 pKa = 11.15ANGDD109 pKa = 3.42LHH111 pKa = 7.27GVGLTGLGDD120 pKa = 3.41INEE123 pKa = 4.33FVYY126 pKa = 9.41LTSDD130 pKa = 4.42FEE132 pKa = 5.75SNDD135 pKa = 3.44NPGALYY141 pKa = 10.2EE142 pKa = 4.27YY143 pKa = 7.67TTGAFTAYY151 pKa = 10.53ASMMDD156 pKa = 3.63AGDD159 pKa = 4.62DD160 pKa = 3.64YY161 pKa = 11.89GFDD164 pKa = 4.05GDD166 pKa = 5.63NDD168 pKa = 4.66TVWSIGGKK176 pKa = 9.46YY177 pKa = 9.37AASNYY182 pKa = 10.29AFGLGYY188 pKa = 10.34EE189 pKa = 4.3AVDD192 pKa = 3.81VDD194 pKa = 5.75GFDD197 pKa = 3.93LNQWTISGEE206 pKa = 4.33GTFGQATVKK215 pKa = 10.62AIYY218 pKa = 10.5SDD220 pKa = 3.63MDD222 pKa = 3.59NDD224 pKa = 4.81GVFDD228 pKa = 4.56DD229 pKa = 4.1VKK231 pKa = 11.02QYY233 pKa = 11.11GLSVMYY239 pKa = 10.34DD240 pKa = 3.25AGATDD245 pKa = 3.53VSAFYY250 pKa = 10.95KK251 pKa = 10.13KK252 pKa = 10.37DD253 pKa = 3.29EE254 pKa = 4.08YY255 pKa = 11.66SFAGVDD261 pKa = 3.32TGDD264 pKa = 3.47ADD266 pKa = 3.46TWGIGAAYY274 pKa = 10.02DD275 pKa = 3.78LGGGATLKK283 pKa = 11.01GGIVDD288 pKa = 3.52SDD290 pKa = 4.1YY291 pKa = 11.64NADD294 pKa = 3.9TIADD298 pKa = 3.94FGVAFTFF305 pKa = 4.36

Molecular weight: 31.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9ECQ1|A0A2K9ECQ1_9RHOB GNAT family N-acetyltransferase OS=Paracoccus sp. BM15 OX=1529068 GN=CUV01_04280 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41AGRR28 pKa = 11.84LVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3740

0

3740

1166477

30

2290

311.9

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.552 ± 0.062

0.823 ± 0.011

6.454 ± 0.039

5.547 ± 0.037

3.569 ± 0.024

8.87 ± 0.041

2.0 ± 0.019

5.326 ± 0.029

2.779 ± 0.035

9.968 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.022

2.59 ± 0.025

5.228 ± 0.031

3.567 ± 0.024

6.956 ± 0.039

5.103 ± 0.024

5.299 ± 0.029

6.901 ± 0.033

1.476 ± 0.018

2.115 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski